scholarly journals The Influence of 0.03% Carbon Dioxide on Protein Metabolism of Etiolated Avena sativa Coleoptiles

1974 ◽  
Vol 54 (1) ◽  
pp. 19-22 ◽  
Author(s):  
A. W. Bown ◽  
T. Aung
Keyword(s):  
Carbon Dioxide ◽  
Avena Sativa ◽  
Protein Metabolism

The influence of carbon dioxide on protein synthesis in etiolated coleoptiles of Arena sativa

1972 ◽  
Vol 50 (9) ◽  
pp. 1937-1942
Author(s):  
A. W. Bown ◽  
W. W. Lampman
Keyword(s):  
Carbon Dioxide ◽  
Protein Synthesis ◽  
Avena Sativa ◽  
Protein Metabolism ◽  
Protein Fraction ◽  
Carbon Dioxide Concentration ◽  
Electrophoretic Analysis ◽  
Plant Tissues ◽  
Atmospheric Concentration ◽  
Profound Influence

Various concentrations of carbon dioxide have been used to assess the influence of carbon dioxide on protein metabolism in etiolated coleoptiles of Avena sativa. Increases in carbon dioxide concentration from 0% to 0.03% to 3% result in dramatic increases in both the total level of protein and the incorporation of radioactive leucine into protein. In addition an electrophoretic analysis indicates that as the carbon dioxide concentration is raised from 0% to 0.03% there is both an increased synthesis of most proteins plus a pronounced synthesis of one particular protein fraction. These results indicate that the normal atmospheric concentration of carbon dioxide has a profound influence on protein metabolism in Avena sativa coleoptiles and are discussed in connection with the well-documented phenomenon of carbon dioxide stimulated growth in etiolated plant tissues.

Inhibition by carbon dioxide of the phototropic response of the Avena coleoptile to blue light

1987 ◽  
Vol 65 (3) ◽  
pp. 488-490 ◽  
Author(s):  
Gordon I. McIntyre
Keyword(s):  
Carbon Dioxide ◽  
Blue Light ◽  
Avena Sativa ◽  
Water Saturation ◽  
Phototropic Response ◽  
Avena Coleoptile ◽  
Inhibition Of Growth ◽  
Saturated Water ◽  
Coleoptile Elongation

When intact oat coleoptiles (Avena sativa var. Harmon) were submerged in water, saturation of the water with CO2 promoted their elongation but eliminated their phototropic response to blue light. Increasing the pH of the CO2-saturated water prevented the promotion of coleoptile elongation but did not prevent the elimination of the phototropic response. In air, phototropic curvature was significantly reduced by 10% CO2 and was eliminated by 30%, without any reduction in growth. It is postulated that the increase in CO2 concentration may eliminate the phototropic curvature of the coleoptile by preventing the light-induced inhibition of growth on the illuminated side of the organ. Possible mechanisms are briefly discussed.

The presence and role of phosphopyruvate carboxylase in etiolated coleoptiles of Avena sativa

1971 ◽  
Vol 49 (2) ◽  
pp. 321-326 ◽  
Author(s):  
A. W. Bown ◽  
W. W. Lampman
Keyword(s):  
Carbon Dioxide ◽  
Protein Synthesis ◽  
Avena Sativa ◽  
Malic Enzyme ◽  
Carbon Dioxide Fixation

Phosphopyruvate carboxylase and malic enzyme were detected in etiolated coleoptiles of Avena sativa, and it was concluded that the incorporation of 14C-labeled bicarbonate into aspartate and glutamate involved the activity of the former enzyme. IAA stimulated the fixation of labeled bicarbonate, and the incorporation of labeled leucine into protein was increased in the presence of carbon dioxide. It is suggested that the carbon dioxide stimulated growth of Avena coleoptiles is due to an increased rate of protein synthesis which is dependent on carbon dioxide fixation.

An investigation into the influence of IAA and malate on in vivo and in vitro rates of dark carbon dioxide fixation in coleoptile tissue

1977 ◽  
Vol 55 (12) ◽  
pp. 1641-1645 ◽  
Author(s):  
I. J. Dymock ◽  
B. Hill ◽  
A. W. Bown
Keyword(s):  
Carbon Dioxide ◽  
Avena Sativa ◽  
Indoleacetic Acid ◽  
Enzymic Activity ◽  
Time Period ◽  
Rapid Effect ◽  
Bicarbonate Fixation ◽  
Stimulation Of

Etiolated Avena sativa L. cv. Victory coleoptiles were used to determine the influence of indoleacetic acid (IAA) or malate on in vivo and in vitro rates of CO2 fixation. In addition, the influence of malate on IAA-stimulated growth was investigated. Concentrations of malate which stimulate growth did not influence the in vivo rate of dark [14C]bicarbonate fixation but did inhibit in vitro phosphoenolpyruvate carboxylase (EC 4.1.1.31) activity. IAA did not influence this enzymic activity or reduce the inhibition of the enzyme by malate, and the rate of [14C]bicarbonate fixation was not measurably influenced by 20 μM IAA within the time period required for IAA stimulation of growth to become apparent. In the absence of atmospheric levels of CO2, 1 mM malate and 20 μM IAA stimulate growth in a weakly synergistic manner. These results are discussed in relationship to a suggestion that IAA-stimulated H+ secretion and growth involves a rapid effect on CO2 fixation.

Electron Cytochemical Study of Carbon Dioxide Laser Effect on Rhesus Monkey Cornea

1974 ◽  
Vol 32 ◽  
pp. 224-225
Author(s):  
K. C. Tsou ◽  
J. Morris ◽  
P. Shawaluk ◽  
B. Stuck ◽  
E. Beatrice
Keyword(s):  
Carbon Dioxide ◽  
Electron Microscope ◽  
Rhesus Monkey ◽  
Carbon Dioxide Laser ◽  
Cytochemical Study ◽  
Laser Effect

While much is known regarding the effect of lasers on the retina, little study has been done on the effect of lasers on cornea, because of the limitation of the size of the material. Using a combination of electron microscope and several newly developed cytochemical methods, the effect of laser can now be studied on eye for the purpose of correlating functional and morphological damage. The present paper illustrates such study with CO2 laser on Rhesus monkey.

The Critical Point Drying Method Applied to Scanning Electron Microscopy of L-929 Cells

1970 ◽  
Vol 28 ◽  
pp. 278-279
Author(s):  
Charles TurnbiLL ◽  
Delbert E. Philpott
Keyword(s):  
Electron Microscopy ◽  
Carbon Dioxide ◽  
Surface Tension ◽  
Critical Point ◽  
Freeze Drying ◽  
Attractive Alternative ◽  
Crystal Formation ◽  
Critical Point Drying ◽  
Time Required ◽  
Scanning Electron

The advent of the scanning electron microscope (SCEM) has renewed interest in preparing specimens by avoiding the forces of surface tension. The present method of freeze drying by Boyde and Barger (1969) and Small and Marszalek (1969) does prevent surface tension but ice crystal formation and time required for pumping out the specimen to dryness has discouraged us. We believe an attractive alternative to freeze drying is the critical point method originated by Anderson (1951; for electron microscopy. He avoided surface tension effects during drying by first exchanging the specimen water with alcohol, amy L acetate and then with carbon dioxide. He then selected a specific temperature (36.5°C) and pressure (72 Atm.) at which carbon dioxide would pass from the liquid to the gaseous phase without the effect of surface tension This combination of temperature and, pressure is known as the "critical point" of the Liquid.

SEM Cold Stage Techniques for the Observation of Vegetative and Floral Apices of Oat (Avena sativa L.) Plants

1977 ◽  
Vol 35 ◽  
pp. 590-591
Author(s):  
B. K. Kirchoff ◽  
L.F. Allard ◽  
W.C. Bigelow
Keyword(s):  
Critical Point ◽  
Avena Sativa ◽  
Substantial Improvement ◽  
Fresh Tissue ◽  
Cold Stage ◽  
Critical Point Drying ◽  
Almost All ◽  
Cell Collapse ◽  
Conductive Paint ◽  
Carbon Based

In attempting to use the SEM to investigate the transition from the vegetative to the floral state in oat (Avena sativa L.) it was discovered that the procedures of fixation and critical point drying (CPD), and fresh tissue examination of the specimens gave unsatisfactory results. In most cases, by using these techniques, cells of the tissue were collapsed or otherwise visibly distorted. Figure 1 shows the results of fixation with 4.5% formaldehyde-gluteraldehyde followed by CPD. Almost all cellular detail has been obscured by the resulting shrinkage distortions. The larger cracks seen on the left of the picture may be due to dissection damage, rather than CPD. The results of observation of fresh tissue are seen in Fig. 2. Although there is a substantial improvement over CPD, some cell collapse still occurs.Due to these difficulties, it was decided to experiment with cold stage techniques. The specimens to be observed were dissected out and attached to the sample stub using a carbon based conductive paint in acetone.

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