Reductive amination in soybean cotyledons: a survey of aminating dehydrogenases

1972 ◽  
Vol 50 (4) ◽  
pp. 897-900
Author(s):  
W. Yung-Fan Wu ◽  
J. King
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Dehydrogenase Activity ◽  
Reductive Amination ◽  
Nadh Oxidase ◽  
Crude Enzyme ◽  
Nadh Oxidation ◽  
Negative Results ◽  
Glycine Max L

Crude enzyme extracts from green, 11-day-old soybean (Glycine max L. var. Canadian No. 1) cotyledons were examined for aminating dehydrogenase activity. Apparent reductive amination of α-ketoisocaproate, α-ketoisovalerate, pyruvate, and β-hydroxypyruvate observed as an increase of NADH oxidation when either NH4Cl or (NH4)2SO4 was added to reaction mixtures could not be confirmed by paper cochromatography with appropriate authentic amino acids. Increase in NADH oxidation was explained by increases in activity of either non-aminating dehydrogenases or NADH oxidase caused either by a shift in pH when NH4+ was added to assay mixtures or by added Cl− and SO42−. Negative results were also obtained with glyoxylate. Reductive amination was confirmed only with α-ketoglutarate.

Prevalence of the pit and antipit in the genus Glycine

1987 ◽  
Vol 45 ◽  
pp. 986-987
Author(s):  
R. W. Yaklich ◽  
E. L. Vigil ◽  
W. P. Wergin
Keyword(s):  
Amino Acids ◽  
Endoplasmic Reticulum ◽  
Glycine Max ◽  
Seed Coat ◽  
Cell Types ◽  
Abaxial Surface ◽  
Legume Seed ◽  
Initial Report ◽  
Cone Cells ◽  
Glycine Max L

The legume seed coat is the site of sucrose unloading and the metabolism of imported ureides and synthesis of amino acids for the developing embryo. The cell types directly responsible for these functions in the seed coat are not known. We recently described a convex layer of tissue on the inside surface of the soybean (Glycine max L. Merr.) seed coat that was termed “antipit” because it was in direct opposition to the concave pit on the abaxial surface of the cotyledon. Cone cells of the antipit contained numerous hypertrophied Golgi apparatus and laminated rough endoplasmic reticulum common to actively secreting cells. The initial report by Dzikowski (1936) described the morphology of the pit and antipit in G. max and found these structures in only 68 of the 169 seed accessions examined.

Effect of Phosphorus and Sulphur on Nutrient and Amino Acids Content of Soybean (Glycine max L. Merill) under ‘Alfisols’

2017 ◽  
Vol 16 (4) ◽  
pp. 1-7
Author(s):  
Sandeep Kumar ◽  
Javeed Wani ◽  
Bilal Lone ◽  
Asma Fayaz ◽  
Purshotam Singh ◽  
...  
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Glycine Max L

Effect of phosphorus and sulphur on nutrient and amino acids content of soybean [Glycine max (L.) Merill] under ‘Eutrochrepts’

2017 ◽  
Author(s):  
Sandeep Kumar ◽  
Javeed Ahmad Wani ◽  
Narinder Panotra ◽  
Bilal Ahmad Lone ◽  
Sameera Qayoom ◽  
...  
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Block Design ◽  
Soybean Seed ◽  
Omega 3 ◽  
Combined Application ◽  
Glycine Max L ◽  
Four Levels ◽  
Seed Application ◽  
Phosphorus Application

A field experiment was conducted at KVK, Srinagar during two consecutive kharif seasons of 2010 and 2011 to study the “Effect of phosphorus and sulphur on nutrient and amino acids content of soybean [Glycine max (L.) Merill] under Eutrochrepts”. The experiment was laid down under 16 treatment combinations viz four levels of phosphorus (0, 30, 60, 90 kg P2O5 ha-1) and four levels of sulphur (0, 15, 30, 45 kg S ha-1) in randomized complete block design with three replication . At higher levels of phosphorus application, Zn content of seed decreased and it was maximum at 30 kg P2O5 ha-1. With application of 45 kg S ha-1, N, P, K, Ca, Mg and S content in seed was 6.54, 0.555, 1.881, 0.329, 0.434 and 0.501 per cent respectively while as Zn, Fe, Cu, Mn was 109.99, 99.96, 2.82 and 3.73 mg kg-1, respectively. A significant interaction between P and S on macro as well as micronutrient content except Zn in seed was observed. Combined application of phosphorus and sulphur further enhanced the nutrient content of soybean seed. Combined application of phosphorus and sulphur enhanced the crude protein and oil content in soya seed 1. Individual as well as interaction effect of P and S was significant in enhancing the sulphur containing amino acids viz., cystine cystein and methionine content of soybean seed. Combined application of 45 kg S and 90 kg P2O5 ha-1 recorded significantly higher carbohydrate content (23.49%) in soybean seed. Application of increasing levels of phosphorus and sulphur resulted in gradual increase in linoleic (Omega-6) and linolenic acid (Omega-3).

Regulation of the accumulation of β-Subunit of β-Conglycinin in nitrogen starved soybean (Glycine max L.) seed by amino acids and organic acids

2001 ◽  
pp. 132-133 ◽  
Author(s):  
N. Ohtake ◽  
S. Okano ◽  
T. Kawachi ◽  
H. Fujikake ◽  
K. Sueyoshi ◽  
...  
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Organic Acids ◽  
Β Subunit ◽  
Glycine Max L

Amino acid transport systems of cultured soybean root cells

1975 ◽  
Vol 53 (18) ◽  
pp. 2088-2091 ◽  
Author(s):  
J. King ◽  
Rozina Hirji
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Transport Systems ◽  
Acid Transport ◽  
Root Cells ◽  
Soybean Root ◽  
Neutral Amino Acids ◽  
Glycine Max L

The uptake of 1 μM14C-labelled arginine, glutamate, and alanine by cultured soybean (Glycine max L. cv. Mandarin) root cells was followed for periods up to 4 min at pH 5.5 in the presence of a 10 μM concentration of other amino compounds. From the degree of competition between 14C-labelled and unlabelled amino acids a number of uptake systems for basic, acidic, and neutral amino acids were identified, and a number of problems associated with amino acid transport in soybean cells were uncovered.

Investigations of growth-promoting factors in conditioned soybean root cells and in the liquid medium in which they grow: cytokinin-like compounds

1974 ◽  
Vol 52 (12) ◽  
pp. 2459-2463 ◽  
Author(s):  
P. A. Sargent ◽  
J. King
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Liquid Medium ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Plant Tissues ◽  
Root Cells ◽  
Soybean Cotyledon ◽  
Glycine Max L ◽  
Growth Promoting

By using the soybean cotyledon callus bioassay for cytokinin-like compounds and two methods of extraction of cytokinins from plant tissues, the presence of growth-active compounds of a purine nature has been detected in soybean (Glycine max. L. cv. Mandarin) root cell suspension cultures growing in a medium containing NH4+. These compounds can be added to residual NH4+, glutamine, and an adequate supply of amino acids as conditioning substances in these cultures.

Photosynthetic assimilation and translocation of 3H- and 14C-organic compounds after 3HHO and 14CO2 were simultaneously offered to a primary leaf of soybean

1971 ◽  
Vol 49 (5) ◽  
pp. 757-766 ◽  
Author(s):  
Robert G. Thompson ◽  
C. D. Nelsons
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Organic Acids ◽  
Glutamic Acid ◽  
Organic Compounds ◽  
Primary Leaf ◽  
Main Compound ◽  
Glycine Max L ◽  
Photosynthetic Assimilation

After assimilation of both 3HHO and 14CO2 by soybean (Glycine max L.) in the light, the percentage of nonexchangeable 3H was similar to the percentage of 14C distributed in each of the identified sugars, amino acids, and organic acids with the exceptions of glycine–serine and glutamic acids. Glycine–serine contained proportionally more 14C than expected on the basis of percentage of 3H; and glutamic acid contained proportionally more 3H than expected on the basis of 14C. Both 3H- and 14C-organic compounds were translocated in the same proportions to various parts of the plant and in both cases the main compound translocated was apparently sucrose. The metabolism of dark-assimilated 3H was different from dark-assimilated 14C, but in both cases it did not contribute significantly to the total metabolism of the plant.

Kinetics of free protein amino acids, free non-protein amino acids and trigonelline in soybean (Glycine max L.) and lupin (Lupinus angustifolius L.) sprouts

2006 ◽  
Vol 224 (2) ◽  
pp. 177-186 ◽  
Author(s):  
Cristina Martínez-Villaluenga ◽  
Yu-Haey Kuo ◽  
Fernand Lambein ◽  
Juana Frías ◽  
Concepción Vidal-Valverde
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Lupinus Angustifolius ◽  
Free Protein ◽  
Glycine Max L ◽  
Protein Amino Acids ◽  
Kinetics Of
Sign in / Sign up

Export Citation Format

Share Document