Effects of nitrogenous materials on translocation and stump exudation in root systems of tomato

1968 ◽  
Vol 46 (4) ◽  
pp. 363-376 ◽  
Author(s):  
Wm. Harold Minshall

An application of urea or of potassium nitrate to potted tomato plants in the 11- to 18-leaf stage of growth increased markedly the quantity of solutes in the stump exudate for the next 48 to 72 hours. This increase of solutes was most pronounced during the maximum part of the diurnal rhythmic cycle. A large proportion of the increased quantity of solutes was composed of asparagine, glutamine, aspartic acid, glutamic acid, nitrate, ammonium, etc. and thus directly related to the metabolism of the applied nitrogen. In addition, however, increased quantities of phosphorus and, at certain times, of potassium were noted.In detopped tomato plants the increased transfer of solutes from the treatment with nitrogenous materials increased the maximum rate of stump exudation from 2 ml/(plant × hour) for water control plants to 8 ml/(plant × hour). Single plants produced 80 ml of stump exudate in 24 hours. The time course effect on rate of exudation by the two forms of nitrogen differed in that potassium nitrate produced its maximum effect in from 6 to 8 hours following an addition at 0830 hours but urea produced its maximum effect in from 28 to 30 hours. By the fourth day the rate of exudation from nitrogen-treated plants was approximately the same as from water control plants.The Q10 for the rate of exudation of plants treated with nitrogenous compounds varied from 1.6 to 5.2 with an average value of 3.0. As the soil temperature was decreased from 18° to 8 °C the osmotic pressure of the stump exudate increased and the difference was statistically significant at the 1% level. Between soil temperatures of 18° and 28 °C, however, the difference in osmotic pressure of the exudates were not statistically significant. A reduced transport of water by physical means is suggested as the cause of the increase in osmotic pressure of the stump exudate at 8 °C.When plants treated with nitrogenous materials were compared to water control plants no support was found for the theory that active mechanisms participated in the transport of the water. In fact the marked increase in the osmotic pressure of the augmented exudates indicated that a lag existed in the movement of the water.It was concluded that the addition of nitrogenous materials to the soil increased by active processes the movement of solutes into the xylem sap of the plants. By osmosis the increased quantity of solutes then increased the quantity of water transported through the roots and collected as stump exudate.

Weed Science ◽  
1969 ◽  
Vol 17 (2) ◽  
pp. 197-201 ◽  
Author(s):  
Wm. Harold Minshall

An application of potassium nitrate or urea to the soil of detopped potted tomato plants [Lycopersicon esculentum Mill.] increased the rate of exudation from the stumps of the plants from 100 to over 300% and increased the concentration of 2-chloro-4-ethylamino-6-isopropylamino-s-triazine [atrazine] in this augmented exudate from 9 to 40%. Atrazine applied to the soil at 2:00 PM was detected in the stump exudate within 10 min and with an application of 3500 μg per pot it was approaching its maximum concentration by the end of 3 hr. The build-up in the concentration of atrazine in the exudate occurred at a faster rate in plants treated with potassium nitrate than in water control plants. Increasing the soil temperature from 10 to 30 C increased the rate of exudation and at each temperature an application of potassium nitrate or of urea increased the concentration of atrazine in the augmented exudate. In addition to atrazine, 2-chloro-4,6-bis(isopropylamino)-s-triazine [propazine], 2-methoxy-4,6-bis(isopropylamino)-s-triazine [prometone], and 2-methoxy-4-ethylamino-6-isopropylamino-s-triazine [atratone] were applied at 1750 and 3500 μg/pot. The concentration of these triazines in the exudate increased directly with their solubility in water. An application of urea increased the concentration of all four triazines in the exudate.


2013 ◽  
Vol 57 (3) ◽  
pp. 1434-1441 ◽  
Author(s):  
Dong-Gun Lee ◽  
Yoichi Murakami ◽  
David R. Andes ◽  
William A. Craig

ABSTRACTReduced bactericidal efficacy at a high inoculum is known as the inoculum effect (IE). We used neutropenic mice to compare the IEs of ceftobiprole (CFB), daptomycin (DAP), linezolid (LZD), and vancomycin (VAN) against 6 to 9 strains ofStaphylococcus aureusand 4 strains ofStreptococcus pneumoniaeat 2 inocula in opposite thighs of the same mice. Neutropenic mice had 104.5to 105.7CFU/thigh (low inoculum [LI]) in one thigh and 106.4to 107.2CFU/thigh (high inoculum [HI]) in the opposite thigh when treated for 24 h with subcutaneous (s.c.) doses every 12 h of DAP at 0.024 to 100 mg/kg of body weight and LZD at 0.313 to 320 mg/kg and s.c. doses every 6 h of CFB at 0.003 to 160 mg/kg and VAN at 0.049 to 800 mg/kg. Dose-response data were analyzed by a maximum effect (Emax) model using nonlinear regression. Static doses for each drug and at each inoculum were calculated, and the difference between HI and LI (IE index) gave the magnitude of IE for each drug-organism combination. Mean (range) IE indexes ofS. aureuswere 2.9 (1.7 to 4.6) for CFB, 4.1 (2.6 to 9.3) for DAP, 4.6 (1.7 to 7.1) for LZD, and 10.1 (6.3 to 20.3) for VAN. InS. pneumoniae, the IE indexes were 2.5 (1.3 to 3.3) for CFB, 2.0 (1.6 to 2.8) for DAP, 1.9 (1.7 to 2.2) for LZD, and 1.5 (0.8 to 3.2) for VAN; these values were similar for all drugs. InS. aureus, the IE was much larger with VAN than with CFB, DAM, and LZD (P< 0.05). Anin vivotime course of vancomycin activity showed initiation of killing at 4- to 16-fold-higher doses at HI than at LI despite similar initial growth of controls.


1984 ◽  
Vol 11 (5) ◽  
pp. 351 ◽  
Author(s):  
R Munns ◽  
JB Passioura

Barley (salt-tolerant) and white lupin (salt-sensitive) were grown in sand in pots designed to fit within a pressure chamber. The sand was irrigated with a nutrient solution to which increasing amounts of NaCl were added daily in increments of 10-25 mol m-3. For a range of transpiration rates (Q), the hydrostatic pressure of the leaf xylem sap of an intact plant was measured by applying sufficient air pressure (p) to the root system to raise the pressure of this sap to zero. The relation between p and Q was linear, i.e. of the form p = po + rQ. Po, the intercept on the p axis, reflects the difference in osmotic pressure across the root, and it is assumed that r, the slope of this relation, gives the hydraulic resistance of the plant. In NaCl-treated barley, r remained constant as the NaCl in the soil solution was increased to 200 mol m-3 over 10 days, and differed little from that of the controls. Po increased by about the same amount as the increase in osmotic pressure of the soil solution. This indicates near-perfect osmotic behaviour by the roots, and consistent with this, osmotic pressure of sap expressed from the cut shoot base generally changed little with increasing NaCl, for a given transpiration rate. In NaCl-treated lupin, by contrast, r increased continually from 25 to 150 mol m-3 NaCI, when it was four times that of the controls. Beyond 75 mol m-3, po increased less than increases in the osmotic pressure of the soil solution, which suggests that salts were then leaking into the root xylem. However, the osmotic pressure of the xylem sap flowing through the petiole did not start to increase until 3 days later when the external solution was over 120 mol m-3, suggesting that substantial amounts of NaCl were being removed from the xylem sap before it reached the petiole.


Author(s):  
P. Maupin-Szamier ◽  
T. D. Pollard

We have studied the destruction of rabbit muscle actin filaments by osmium tetroxide (OSO4) to develop methods which will preserve the structure of actin filaments during preparation for transmission electron microscopy.Negatively stained F-actin, which appears as smooth, gently curved filaments in control samples (Fig. 1a), acquire an angular, distorted profile and break into progressively shorter pieces after exposure to OSO4 (Fig. 1b,c). We followed the time course of the reaction with viscometry since it is a simple, quantitative method to assess filament integrity. The difference in rates of decay in viscosity of polymerized actin solutions after the addition of four concentrations of OSO4 is illustrated in Fig. 2. Viscometry indicated that the rate of actin filament destruction is also dependent upon temperature, buffer type, buffer concentration, and pH, and requires the continued presence of OSO4. The conditions most favorable to filament preservation are fixation in a low concentration of OSO4 for a short time at 0°C in 100mM sodium phosphate buffer, pH 6.0.


Author(s):  
Yunita Yunita ◽  
Hidayat Hidayat ◽  
Harun Sitompul

This study aims to: (1) investigate the effect of Jigsaw cooperative learning on students learning outcomes; (2) find the difference in learning outcomes between high and low learning motivation and (3) find the interaction between learning approaches and learning motivation towards learning outcomes. The population of the study is students of grade IVa, IVb, IVc at SD Kasih Ibu Patumbak and the sample in this study is grade IVa with 35 students and grade IVb with 35 students. The results show that: (1) the average student learning outcomes of jigsaw cooperative learning is 28.40 while conventional is 24.14. Thus, students learning outcomes that get cooperative learning of jigsaw type are higher than conventional learning, (2) Students who have high motivation get an average value = 30.74, while low motivation is 22.72. Thus, it can be concluded that there are differences in student learning outcomes having high learning motivation and low learning motivation, and (3) students learning outcomes  taught by jigsaw cooperative learning are high learning motivation groups (32.94), and low learning motivation groups (24.58), while students taught with conventional learning are high learning motivation groups (28.40 ), and low motivation groups (20,95). Thus, there is no interaction between learning approaches and learning motivation towards learning outcomes.


2017 ◽  
Vol 1 (1) ◽  
pp. 89
Author(s):  
Indah Purwaningsih ◽  
Supriyanto Supriyanto

Abstract: Chlorine is a green halogen-shaped halogen gas at normal temperature and serves as bleach, stain remover and disinfectant. Chlorine is now widely used for bleaching rice so that less quality rice looks like quality rice. Chlorine is very toxic and causes mucous membrane irritation, highly reactive and very powerful oxidizer. The purpose of this research was to determine the difference of chlorine level in chlorinated rice washed once, twice and 3 times. The sample in this study amounted to 11 samples calculated by replication formula. Each sample was treated 3 times, ie 1 washed once, 2 washed twice and washed 3 times. The samples then examined by iodometric titration method. Based on the results of the study using ANOVA test, 11 samples obtained the average value of chlorine after washed once amount of 0.0176%, after washed twice amount of 0.0111%, and after washed 3 times amount of 0.0052% with the value significance p = 0.03 (p <0.05) at 95% confidence level which means there was a significant difference between chlorine levels in chlorinated rice washed once, twice and 3 times.Abstrak: Klorin merupakan unsur halogen berbentuk gas berwarna kuning kehijauan pada suhu normal danberfungsi sebagai pemutih, penghilang noda maupun desinfektan. Klorin sekarang banyak digunakan untuk bahan pemutih beras agar beras yang kurang berkualitas tampak seperti beras berkualitas. Klorin sangat toksik dan menyebabkan iritasi membran mukosa, sangat reaktif dan merupakan oksidator yang sangat kuat. Tujuan dari penelitian ini ialah untuk mengetahui perbedaan kadar klorin pada beras berklorin yang dicuci sebanyak 1 kali, 2 kali dan 3 kali. Sampel dalam penelitian ini berjumlah 11 sampel yang dihitung dengan rumus replikasi. Setiap sampel diberi perlakuan sebanyak 3 kali, yaitu 1 kali pencucian, 2 kali pencucian dan 3 kali pencucian. Sampel penelitian kemudian diperiksa dengan metode titrasi iodometri. Berdasarkan hasil penelitian menggunakan uji Anova secara komputerisasi terhadap 11 sampel diperoleh nilai rata-rata kadar klorin setelah 1 kali pencucian sebesar 0,0176 %, setelah 2 kali pencucian sebesar 0,0111 %, dan setelah 3 kali pencucian sebesar 0,0052 % dengan nilai signifkansi p = 0,03 (p<0,05) pada tingkat kepercayaan 95% yang artinya ada perbedaan yang bermakna antara kadar klorin pada beras berklorin yang dicuci sebanyak 1 kali, 2 kali dan 3 kali.


Author(s):  
Vokulova Yu.A. Vokulova ◽  
E.N. Zhulev

This article presents the results of studying the dimensional accuracy of the bases of complete removable prostheses made using a 3D printer and the traditional method. Bases of complete removable prostheses were made using an intraoral laser scanner iTero Cadent (USA) and a 3D printer Asiga Max UV (Australia). To study the dimensional accuracy of the bases of complete removable prostheses, we used the DentalCAD 2.2 Valletta software. The Nonparametric Wilcoxon W-test was used for statistical analysis of the obtained data. We found that the average value of the difference with the standard for bases made using digital technologies is 0.08744±0.0484 mm. The average value of the difference with the standard for bases made by the traditional method is 0.5654±0.1611 mm. Based on these data, we concluded that the bases of complete removable prostheses made using modern digital technologies (intraoral laser scanning and 3D printer) have a higher dimensional accuracy compared to the bases of complete removable prostheses made using the traditional method with a significance level of p<0.05 (Wilcoxon's W-test=0, p=0.031). Keywords: digital technologies in dentistry, digital impressions, intraoral scanner, 3D printing, ExoCAD, complete removable dentures.


Soft Matter ◽  
2021 ◽  
Author(s):  
Reinhard Höhler ◽  
Jordan Seknagi ◽  
Andrew Kraynik

The capillary pressure of foams and emulsions is the difference between the average pressure in the dispersed phase and the pressure in the continuous phase.


2021 ◽  
Vol 9 (6) ◽  
pp. 1184
Author(s):  
Nomfusi Ntsobi ◽  
Morris Fanadzo ◽  
Marilize Le Roes-Hill ◽  
Felix Nchu

Globally, fungal inocula are being explored as agents for the optimization of composting processes. This research primarily evaluates the effects of inoculating organic vegetable heaps with the entomopathogenic fungus Clonostachys rosea f. catenula (Hypocreales) on the biophysicochemical properties of the end-product of composting. Six heaps of fresh cabbage (Brassica oleracea var. capitata) waste were inoculated with C. rosea f. catenula conidia and another six were not exposed to the fungus. The composted materials from the fungus- and control-treated heaps were subsequently used as a medium to cultivate tomatoes (Solanum lycopersicum). The biophysicochemical characteristics of the composted materials were also assessed after composting. In addition, the protective effect of the fungal inoculum against red spider mite (Tetranychus urticae) infestations in the tomatoes was evaluated through the determination of conidial colonization of the plant tissue and the number of plants infested by the insect. Furthermore, phytotoxicity tests were carried out post experiment. There were few significant variations (p < 0.05) in heap temperature or moisture level between treatments based on the weekly data. We found no significant differences in the levels of compost macronutrient and micronutrient constituents. Remarkably, the composted materials, when incorporated into a growth medium from fungus-treated heaps, induced a 100% endophytic tissue colonization in cultivated tomato plants. While fewer red spider mite infestations were observed in tomato plants grown in composted materials from fungus-treated heaps, the difference was not significant (χ2 = 0.96 and p = 0.32). The fungal treatment yielded composted materials that significantly (p < 0.05) enhanced tomato seed germination, and based on the phytotoxicity test, the composted samples from the heaps exposed to the C. rosea f. catenula inoculum were not toxic to tomato seeds and seedlings. In conclusion, this study showed that C. rosea f. catenula improved the quality of composted materials in terms of fungal endophytism and seed germination.


Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 292
Author(s):  
Ilie Bodale ◽  
Gabriela Mihalache ◽  
Vladut Achiţei ◽  
Gabriel-Ciprian Teliban ◽  
Ana Cazacu ◽  
...  

Nutrient consumption by plants depends on the growth stage and environmental conditions. In general, plants take up species of elements at different speeds. We monitored and recorded the electrical charge flow through xylem sap of tomato plants (Brillante F1) using femto/picoammeter equipment (Keysight B2981A). This technique evaluates the nutrient uptake of tomato treated with the most common macronutrients (KNO3; KH2PO4; Ca(NO3)2; KCl) by monitoring the electrical conductivity for 24 h. The electrical conductivity of each treatment correlated with the plant growth and development stages. The results showed that the tomato plants had a high consumption of nutrients in the vegetative stage, while in other stages, they had a specific consumption, like phosphorus for bulb formation, potassium for increasing the number of flowers and water for the ripening of fruits. The quantitative evaluation of the ions absorbed by the plant was based on the magnitude and shape of the electrical conductivity curves. Our technique is an efficient method to determine nutrient consumption and is useful in predicting the deficiency of a certain element in tomato plants.


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