In vitro culture of a dwarf mistletoe, Arceuthobium pusillum

1968 ◽  
Vol 46 (2) ◽  
pp. 161-164 ◽  
Author(s):  
J. M. Bonga ◽  
C. Chakraborty
Keyword(s):  
In Vitro Culture ◽  
Casein Hydrolysate ◽  
Coconut Milk ◽  
Dwarf Mistletoe ◽  
Anatomical Characteristics ◽  
Original Length

Embryos, small seedlings, and seeds of Arceuthobium pusillum Peck were cultured on White's medium supplemented with coconut milk and casein hydrolysate. The embryos increased in size up to five times their original length and often produced a holdfast at the tip of the radicle. Holdfasts with haustorial wedges were produced in cultures of small seedlings. In some of the seed cultures the radicles produced branches which showed many of the anatomical characteristics typical of young branches of the natural endophytic system inside the host bark. Occasionally a callus mass was formed at the base of the radicle and on the seed.

scholarly journals Establishment and development of the Catherine’s moss Atrichum undulatum (Hedw.) P. Beauv. (Polytrichaceae) in in vitro conditions

2006 ◽  
Vol 58 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Aneta Sabovljevic ◽  
Tijana Cvetic ◽  
M. Sabovljevic
Keyword(s):  
In Vitro Culture ◽  
Ms Medium ◽  
Mineral Salts ◽  
Anatomical Characteristics

The effect of sucrose and mineral salts on morphogenesis of the Catherine?s moss (Atrichum undulatum) in in vitro culture was tested. In vitro culture of this species was established from disinfected spores on Murashige and Skoog (MS) medium. Apical shoots of gametophytes were used to investigate the influence of sucrose and mineral salts on protonemal and gametophyte growth and multiplication. Paper also treats morpho-anatomical characteristics of plants grown in nature and plants derived from in vitro culture.

The morphology and anatomy of holdfasts and branching radicles of Arceuthobium pusillum cultured in vitro

1969 ◽  
Vol 47 (12) ◽  
pp. 1935-1938 ◽  
Author(s):  
J. M. Bonga
Keyword(s):  
Indoleacetic Acid ◽  
Casein Hydrolysate ◽  
Dichlorophenoxyacetic Acid ◽  
Dwarf Mistletoe ◽  
Flat Structures ◽  
Meristematic Activity ◽  
Milk Casein ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Center Area

Seeds of the eastern dwarf mistletoe, Arceuthobium pusillum Peck, were cultured on White's medium supplemented with coconut milk, casein hydrolysate, and indoleacetic acid (IAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Large holdfasts were obtained with 2,4-D. At first, growth of these holdfasts arose from a centrally located meristem, but later from growth centers near the periphery of the holdfasts. Reticulate thickening of the cell walls was observed in the center area of the holdfasts. With IAA, flat structures with branches or papillae were obtained. If the seeds were cultured with 2,4-D for 3 months and then with IAA, a number of holdfasts formed branches. Centers of meristematic activity developed occasionally where radicles and branches were subjected to pressure.

scholarly journals Rapid in vitro Regeneration and Clonal Propagation of the Fastest Growing Leguminous Tree Albizia falcataria (L.) Fosberg using Leaflet Explant

2010 ◽  
Vol 20 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Nabarun Ghosh ◽  
Don W. Smith ◽  
A. B. Das ◽  
A. Chatterjee
Keyword(s):  
Clonal Propagation ◽  
In Vitro Regeneration ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Coconut Milk ◽  
Shoot Buds ◽  
Adventitious Shoot Buds ◽  
Albizia Falcataria

Young leaflets were used as the explants for in vitro regeneration of Albizia falcataria (L.) Fosberg without callus intervention. The leaf explants produced in vitro adventitious shoot buds directly on culturing with MS supplemented with BA, IBA (4.0/0.05 mg/l) and 10% coconut milk (v/v). Addition of casein hydrolysate and coconut milk increased the production of shoot buds. The buds produced shoots and roots and showed 66% survival in a field trial. This technique offers an effective way by which large number of genetically stable plants can be produced, maintained, multiplied and transported as disease free propagules or regenerants, safely and economically. Key words: Albizia falcataria, Leaf explant, Clonal propagation, Cytology D.O.I. 10.3329/ptcb.v20i1.5966 Plant Tissue Cult. & Biotech. 20(1): 63-72, 2010 (June)

In vitro culture of the flax rust, Melampsora lini

1970 ◽  
Vol 48 (4) ◽  
pp. 773-776 ◽  
Author(s):  
M. D. Coffey ◽  
A. Bose ◽  
Michael Shaw
Keyword(s):  
Ambient Temperature ◽  
In Vitro Culture ◽  
Yeast Extract ◽  
Coconut Milk ◽  
Melampsora Lini ◽  
Different Strains ◽  
Transfer Procedures ◽  
Inorganic Constituents

Two different strains of Melampsora lini (Pers.) Lév. were grown on media similar to those described by Turel (Can. J. Bot. 47: 821–823). Alterations in organic and inorganic constituents did not, in general, prevent growth. Peptone and yeast extract, singly or in combination, supported growth, but coconut milk did not. Maintenance of the ambient temperature below 17 °C during transfer procedures increased the number of colonies which grew. The mycelium was generally binucleate. Spore-like cells of varying shapes were found, some of which resembled uredospores and teliospores.

Isohexenylnaphthazarins from Lithospermum canescens (Michx.) Lehm. and Arnebia euchroma (Royle) Jonst. in vitro culture

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Graikou ◽  
H Damianakos ◽  
K Syklowska-Baranek ◽  
A Pietrosiuk ◽  
M Jeziorek ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Arnebia Euchroma ◽  
Lithospermum Canescens

Effect of antioxidants on in vitro culture of pomegranate cv. Sindhuri

2018 ◽  
Vol 34 (2) ◽  
pp. 311-318
Author(s):  
Ravi Kumar ◽  
◽  
M.L. Jakhar ◽  
Komal Sekhawat ◽  
Swarnlata Kumawat ◽  
...  
Keyword(s):  
In Vitro Culture
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