CHANGES IN PEACH TREES (cv. RED HAVEN) ATTACKED BY TAPHRINA DEFORMANS, WITH PARTICULAR REFERENCE TO NITROGEN METABOLISM IN INFECTED AND NON-INFECTED LEAVES

1967 ◽  
Vol 45 (4) ◽  
pp. 459-477 ◽  
Author(s):  
Vittorio Raggi
Keyword(s):  
Amino Acids ◽  
Nitrogen Metabolism ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dry Weight ◽  
Infected Tissue ◽  
Diseased Plant ◽  
Glycolytic Activity ◽  
Protein Amino Acids ◽  
Peach Trees

Some of the changes induced by Taphrina deformans on metabolism (particularly the nitrogen metabolism) of peach leaves were studied. Observations were as follows. (1) Dry weight diminishes in the infected tissues, especially at the end of the cycle, whereas it remains almost unchanged in the non-infected tissues of the diseased plant. (2) Initially, the amount of total nitrogen, per gram of dry substance is clearly greater in the infected tissues than in the control, then it becomes lower during the differentiation of the asci, especially at the stage of full sporulation. Similar variations occur also, in a more attenuated form, in the non-infected tissues. (3) A considerable increase of the glycolytic activity is observed in the infected tissue especially during the period preceding the differentiation of the asci, and an increase of respiration, even though much more restricted, is observed also in the next period. In non-invaded tissues, an increase of glycolysis is noted along with a diminution of respiration. (4) The non-infected leaves weigh more than those of the control until the differentiation of the asci; after it, they have a clear drop up to the maturity of the asci, and a further revival at the end of the disease. (5) In the infected tissues, the content of free amino acids is always higher than in the control (with a maximum after sporulation), whereas in the non-infected ones it is generally lower. (6) The more important alterations in the pool of the free amino acids in invaded tissues consist in a scarcity, in percentage of the total, of alanine and glutamic acid, especially at the end of the disease, whereas proline, ornithine, glycine, and others increase, especially during the incubation period. In particular the glutamine–asparagines– threonine group reaches the highest value when sporulation has occurred. In the non-invaded tissues, the changes are more restricted. (7) The content of protein amino acids in infected tissues is generally lower than in the control, especially in the last phases of infection whereas in the non-invaded parts it is generally higher, except in differentiation of the asci. (8) In the infected tissue, the alterations of the quantitative relationships between the single protein amino acids (in percentage of the total) are not very great, but some diminutions and increases are recorded (diminution of alanine, glycine, leucine, etc.; increase of lysine, histidine, etc.); in the non-infected tissues, no noteworthy alterations were observed.

Distribution of cellular amino acids, protein, and total organic nitrogen during fruitbody development in Flammulina velutipes. I. Growth on sawdust medium

1982 ◽  
Vol 60 (8) ◽  
pp. 1330-1341 ◽  
Author(s):  
Hans E. Gruen ◽  
William M. Wong
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Organic Nitrogen ◽  
Amino Nitrogen ◽  
Dry Weight ◽  
Flammulina Velutipes ◽  
Total Organic Nitrogen ◽  
Protein Amino Acid ◽  
Protein Amino Acids

Dry weights of stipes and pilei of Flammulina velutipes grown on nutrient-supplemented sawdust increased throughout the growth period. The pilei weighed only slightly less than the stipes. Amounts of total organic nitrogen, α-amino nitrogen, and alkali-soluble protein increased in the whole pileus and stipe as the fruitbodies elongated, but the concentrations on a dry weight basis decreased although they were always highest in the pileus. The concentration of alkali-insoluble nitrogen increased in both structures and was highest in the stipe. Concentrations of total nitrogen and protein in surface mycelium did not change significantly when fruitbodies formed but increased markedly when their growth ceased. An average of 8.3 mg dry weight of spores containing about 0.6 mg of nitrogen was released during the life of the fruitbody. Changes in 18 free and 17 protein amino acids were followed quantitatively in the pileus and stipe. Glutamic and aspartic acids and alanine were always among the four predominant free amino acids. Lysine and arginine concentrations remained low in the stipes but increased considerably in the expanding pilei. Ornithine levels increased strongly in the stipe during the early part of rapid elongation but remained almost constant and low in the pilei. Free proline was detected only in traces. Valine became the most abundant protein amino acid during elongation, especially in the stipe. There was very little bound methionine and cystine. In surface mycelium levels of free amino acids were low before fruiting and close to the end of fruitbody growth. Protein amino acids increased during that interval but their proportions remained virtually unchanged and valine was not predominant. The concentration of urea remained very low in both pilei and stipes during their growth.

scholarly journals Composition and content of free amino acids of sublittoral red algae from Murmansk coast of the Barents Sea.

2020 ◽  
Vol 11 (5-2020) ◽  
pp. 92-102
Author(s):  
M.P. Klindukh ◽  
◽  
I.V. Ryzhik ◽  
E.O. Dobychina ◽  
◽  
...  
Keyword(s):  
Amino Acids ◽  
Red Algae ◽  
Free Amino Acids ◽  
Free Amino ◽  
Barents Sea ◽  
Qualitative Composition ◽  
The Barents Sea ◽  
Protein Amino Acids

Data on the content of free amino acids (FAA) in four species of red algae are presented. The content of 20 amino acids was determined in the composition of FAA of the studied species. The largest amounts contained glutamic and aspartic acids, alanine, proline and taurine. The studied algae do not differ in the qualitative composition of protein amino acids, but differ in the presence and content of nonprotein ones. The content of most of the FAA and their summ was the highest for Phycodrysrubens, and in Palmaria palmatathe smallest.

The causes of buoyancy in eggs of marine teleosts

1987 ◽  
Vol 67 (1) ◽  
pp. 169-182 ◽  
Author(s):  
J. C. A. Craik ◽  
S. M. Harvey
Keyword(s):  
Amino Acids ◽  
Water Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Sea Water ◽  
Inorganic Ions ◽  
Dry Weight ◽  
High Water Content ◽  
Demersal Eggs ◽  
Pelagic Eggs

Pelagic eggs and demersal eggs of teleosts both have osmotic concentrations similar to that of the maternal body fluids, less than half that of sea water. Pelagic eggs are buoyant because they contain such large quantities of this dilute aqueous fluid. While the demersal eggs of teleosts usually have a water content of 60–70%, the buoyant pelagic eggs of marine teleosts such as whiting, Norway pout, saithe, cod, haddock, turbot, dab, plaice, witch, long rough dab, halibut and sole typically have a very high water content (ca. 92 %) and a lipid content of 10–17% of egg dry weight. About 90% of the buoyancy of such eggs in sea water is caused by their high aqueous content, only about 10% being caused by lipid. The buoyant eggs of grenadier and ling have large oil globules and higher lipid contents, 27 and 35 % of dry weight respectively. Nevertheless, most of their buoyancy is provided by their high aqueous contents (89 and 81 % water). The high water content of pelagic eggs is brought about by a massive influx of water into the oocytes during meiotic maturation (ripening) after vitellogenesis but before ovulation. In cod and plaice, ripening is accompanied by a four- to five-fold increase in both water content and free amino-acids, and by a large influx of both potassium and sodium. In cod, free amino-acids contribute much more than these inorganic ions to the water influx and to the total osmotic concentration of the mature egg, but in plaice the relative contribution of inorganic ions approaches that of the free amino-acids.

scholarly journals Free amino acids in the panicles of Dactylis glomerata in the course of their development

2015 ◽  
Vol 43 (1) ◽  
pp. 15-25
Author(s):  
T. Dąbrowska
Keyword(s):  
Amino Acids ◽  
Nitrogen Metabolism ◽  
Paper Chromatography ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dactylis Glomerata ◽  
Proline Content ◽  
Proline Level

The composition of free amino acids and proline content in various parts of <i>Dactylis glomerata</i> panicles at different stages of their development were determined by paper chromatography and electrophoresis. The proline level in the spikelets was found to change widely in the course of their development. The results are discussed with reference to the role of proline in the nitrogen metabolism of plants.

scholarly journals Free and Total Amino Acid Composition of Maturing Seed from Six Peanut (Arachis hypogaea L.) Cultivars

1980 ◽  
Vol 7 (1) ◽  
pp. 32-37 ◽  
Author(s):  
S. M. M. Basha ◽  
J. P. Cherry ◽  
C. T. Young
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Arachis Hypogaea ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dry Weight ◽  
Rate Of Change ◽  
Arachis Hypogaea L ◽  
Peanut Cultivars ◽  
Total Amino Acids

Abstract Maturing seeds of six peanut cultivars (Arachis hypogaea L.) varying in protein content at maturity showed differences in rate of change of dry weight, crude protein, and free and total amino acids. Seeds of the high-protein cultivars increased in dry weight and deposited protein at a more rapid rate between immature and low intermediate stages of maturation than did those of the low- and intermediate-protein cultivars. Free amino acid content in seeds classified as low-intermediate maturity from all cultivars was significantly less than was that of those at the immature stage. The greatest change was in seed of the high-protein group. The rate of change of content of selected free amino acids among seed was different for the three groups of cultivars. Similar observations were noted as the content of select total amino acids increased in maturing seeds. Variations in quantities of free amino acids in immature seeds and differences in the rate at which they are incorporated into proteins of seeds from various cultivars suggest that there is genetic variability in the mechanism for synthesis of selected proteins. These differences also indicate the potential for the development of peanut cultivars having seed with nutritionally desirable protein.

scholarly journals Endogenous nitrogen metabolism and plasma free amino acids in young adults given a ‘protein-free’ diet

1968 ◽  
Vol 22 (1) ◽  
pp. 9-20 ◽  
Author(s):  
V. R. Young ◽  
N. S. Scrimshaw
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Nitrogen Metabolism ◽  
Free Amino Acids ◽  
Free Amino ◽  
Essential Amino Acids ◽  
Whole Body ◽  
Protein Requirements ◽  
Protein Free Diet ◽  
Plasma Free Amino Acids

1. Variation in endogenous nitrogen metabolism was determined by giving eleven healthy men, aged 17–22, a diet supplying daily only 6 mg N/kg body-weight. Eight subjects were given the diet for 7–10 days and three other subjects were given it for 16 days.2. Body cell mass (BCM) was calculated from whole-body 40K in ten subjects and basal metabolism was determined in seven subjects during the ‘protein-free’ period. Urine was analysed daily for N and creatinine, and faecal N was measured in pooled samples. Plasma free amino acids, serum albumin and protein were measured in preprandial morning blood samples at the beginning and end of the study.3. BCM did not change during the ‘protein-free’ period and accounted for 48% of the total body-weight. Basal calorie expenditure amounted to 48 ± 5 kcal/kg BCM per day.4. Mean daily endogenous urinary N excretion in the eight subjects given the ‘protein-free’ diet for 7–10 days was 36·6 ± 3·0 mg N/kg body-weight, 79·4 ± 4·4 mg N/kg BCM and 1·6 ± 0·2 mg N/basal kcal. Endogenous faecal N excretion was 9·9 ± 1·1 mg N/kg body-weight and accounted for 20% of the total endogenous loss. Results obtained with three other subjects given the diet for 16 days were similar.5. Plasma essential amino acids were reduced, glutamic acid, alanine and glycine increased, and the ratio of essential to non-essential amino acids decreased after 7 or 10 days of ‘protein-free’ diet.6. The loss of endogenous N per basal kcal and of faecal N per kg body-weight was lower than the values assumed in the factorial approach to protein requirements by the FAO/WHO (1965) Expert Group on Protein Requirements.

scholarly journals EFFECT OF TWO ORGANIC NUTRIENTS AND NPK ON SOME VEGETATIV GROWTH TRAITS AND ROOT OF GRAPE TRANSPLANTS CV. SUMMER ROYAL

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Abdulraheem & Hadi
Keyword(s):  
Amino Acids ◽  
Root Growth ◽  
Leaf Area ◽  
Surface Area ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dry Weight ◽  
Growth Characteristics ◽  
Mineral Fertilization ◽  
The University

This research was conducted at the university of the University of   Baghdad / Al-Jadireya during the seasons of 2016  to study the effects of applying the root stimulator Essential plus 1-0-1 and spraying   of the Acadian seaweed and  fertilizer (NPK) and their effects on the vegetative and root growth of grape transplants cv. Summer Royal. A factorial experiment was carried out according to the randomized complete block design with three replications .The experiment included 12 treatments, the first treatment (organic nutriment) (A0), included adding the root stimulator (Essential plus 1-0-1) to the soil with 4 ML.L-1 (A1) spraying the substance of the seaweed (Acadian) with of 1 g.L-1 (A2), and interaction (A3) and the second treatment was spraying with mineral fertilizer NPK (TOTAL GRO) with three concentrations (0,1.5,3 g.L-1) and were designated as B0,B1,B2.The organic nutrient treatment A3 significantly influenced all the measured characteristics as compared to the control treatment, as an increase of 84.38 % was achieved and 150 % for the leaf area of the transplant and the free amino acids, and root growth characteristics, as the length of the roots(39.26 %) and  diameter  of the roots (68.26%), as well as the surface area(26.04%) and the dry weight of the roots (48.71%).The mineral fertilization B1 and the B2 significantly impacted all the measured characteristics as compared with control , as the B2 treatment gave the maximum results in increasing the leaf area of the transplant (48.75) and the free amino acids (62.82%) and the root length (31.03%) and the roots diameter (57.05%) and the surface area of the roots (20.07%) and the dry weight of the roots (24.26%). A significant im provment was achieved in the vegetative and root growth characteristics by using organic nutriment with the mineral fertilization fcr grape transplants.  

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