THE INCREASE IN CONCENTRATION OF TWO STRAINS OF POTATO VIRUS X IN FLOATING LEAF DISCS

1963 ◽  
Vol 41 (4) ◽  
pp. 557-568 ◽  
Author(s):  
C. B. Willis ◽  
R. H. Larson ◽  
R. W. Fulton
Keyword(s):  
Potato Virus ◽  
Leaf Tissue ◽  
Potato Virus X ◽  
Close Correlation ◽  
Virus Concentration ◽  
Optimum Temperature ◽  
Nicotiana Tabacum L ◽  
Rate Of Increase ◽  
Yellow Strain ◽  
Floating Leaf

Increases in concentration of two antigenically similar strains (ringspot and yellow) of potato virus X (PVX) in Nicotiana tabacum L. vars. Samsun and Havana 38 and in Nicotiana rustica L. were determined serologically and by local lesion assay. In discs of inoculated leaf tissue floating on Hoagland's solution the rate of increase was greatest at 16° and 20 °C. In Samsun the optimum temperature for the increase in virus concentration was lower for the yellow strain than the ringspot strain. A close correlation between the assay procedures indicated that, as the temperature increased above the optimum for virus increase, either virus synthesis was being inhibited or virus was being denatured. No differences were found where 8- and 16-hour photoperiods were compared to determine their effect on virus increase at 20 °C. Virus concentrations in Samsun and Havana 38 were similar when determined under similar conditions. Concentrations in N. rustica were appreciably higher. In both Nicotiana species the concentration of the yellow strain at any time after inoculation was lower than that of the ringspot strain.

Respiration of tobacco leaves infected with different strains of potato virus X

1969 ◽  
Vol 47 (5) ◽  
pp. 723-730 ◽  
Author(s):  
M. M. Dwurazna ◽  
M. Weintraub
Keyword(s):  
Potato Virus ◽  
Great Increase ◽  
Experimental Period ◽  
Potato Virus X ◽  
Metabolic Inhibitors ◽  
Virus Concentration ◽  
Tobacco Leaves ◽  
Respiration Rates ◽  
Local Lesions ◽  
Different Strains

A study was made of the effects on respiration by four strains of potato virus X varying in the severity of symptoms they produce on tobacco leaves. No effects on respiration rates were observed in either inoculated or systemically infected but symptomless leaves, even though there was a significant increase in virus concentration during the experimental period. However, a significant increase was detected in any leaves on which symptoms developed, this increase being directly correlated with the severity of symptom expression. At the same time a great increase in virus concentration was found. Measurement of respiration patterns of single local lesions showed that an increase in respiration occurred each time a chlorotic and necrotic ring formed in the tissues. The use of metabolic inhibitors indicated that metabolic controls are different in the leaves infected with latent or mild strains, as compared to those infected with the severe, necrotizing strains.

A yellow strain of potato virus X

1959 ◽  
Vol 36 (3) ◽  
pp. 98-104 ◽  
Author(s):  
A. J. Hansen ◽  
R. H. Larson
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Yellow Strain

Detection, spread, and aphid transmission of potato virus S

1974 ◽  
Vol 52 (3) ◽  
pp. 461-465 ◽  
Author(s):  
J. P. MacKinnon
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Aphid Transmission ◽  
Field Trials ◽  
Potato Tubers ◽  
Green Mountain ◽  
Serological Tests ◽  
Potato Virus S ◽  
Nicotiana Tabacum L ◽  
Nicotiana Debneyi

Seventy-two potato tubers of 106 tested from plants exposed 1 year in a field were found infected with potato virus S (PVS) in different tests. Ninety-three percent of these were detected by tuber juice inoculation to Nicotiana debneyi Domin. and 90% by serology of 30-cm plants grown from an eye of such tubers. Sap inoculation to N. debneyi of the same young plants proved to be 96% efficient in detecting the virus, and serological tests at bloom stage were the most efficient of all the tests compared.Tests done on all tubers from 18 plants currently infected with PVS showed that 103 of 116 (89%) were infected, and virtually all eyes from 68 infected tubers produced infected plants.Three years of field trials at Fredericton on the spread of PVS showed that the virus moved into virus-free varieties independently of potato virus X (PVX). In 1970, leaf tests showed that virus-free Netted Gems became 12% infected with PVS; in 1971, spread into Green Mountain, Kennebec, and Sebago was 57, 19, and 9%, respectively; and in 1972, 14% spread occurred in Green Mountain and none in Kennebec or Sebago.Greenhouse experiments on transmission of PVS to potato by Myzus persicae (Sulz.) resulted in 3 of 87 (3.4%) plants becoming infected. Other tests with potato virus Y (PVY) to tobacco, Nicotiana tabacum L. var. Samsun, resulted in 83% transmission.

Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi

2014 ◽  
Vol 58 (4) ◽  
pp. 783-787 ◽  
Author(s):  
N. Cerovska ◽  
H. Plchova ◽  
P. Vaculik ◽  
T. Moravec ◽  
T. Gichner
Keyword(s):  
Dna Damage ◽  
Nicotiana Tabacum ◽  
Host Plant ◽  
Potato Virus ◽  
Potato Virus X ◽  
Nicotiana Tabacum L

scholarly journals Evidence that the Linker between the Methyltransferase and Helicase Domains of Potato Virus X Replicase Is Involved in Homologous RNA Recombination

2009 ◽  
Vol 83 (15) ◽  
pp. 7761-7769 ◽  
Author(s):  
Heidrun-Katharina Draghici ◽  
Mark Varrelmann
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Fluorescent Protein ◽  
Triple Gene Block ◽  
Leaf Tissue ◽  
Potato Virus X ◽  
Wild Type Virus ◽  
Rna Recombination ◽  
Gene Block

ABSTRACT Recombination in RNA viruses, one of the main factors contributing to their genetic variability and evolution, is a widespread phenomenon. In this study, an in vivo assay to characterize RNA recombination in potato virus X (PVX), under high selection pressure, was established. Agrobacterium tumefaciens was used to express in Nicotiana benthamiana leaf tissue both a PVX isolate labeled with green fluorescent protein (GFP) containing a coat protein deletion mutation (ΔCP) and a transcript encoding a functional coat protein +3′-ntr. Coexpression of the constructs led to virus movement and systemic infection; reconstituted recombinants were observed in 92% of inoculated plants. Similar results were obtained using particle bombardment, demonstrating that recombination mediated by A. tumefaciens was not responsible for the occurrence of PXC recombinants. The speed of recombination could be estimated by agroinfection of two PVX mutants lacking the 3′ and 5′ halves of the genome, respectively, with an overlap in the triple gene block 1 gene, allowing GFP expression only in the case of recombination. Ten different pentapeptide insertion scanning replicase mutants with replication abilities comparable to wild-type virus were applied in the different recombination assays. Two neighboring mutants affecting the linker between the methyltransferase and helicase domains were shown to be strongly debilitated in their ability to recombine. The possible functional separation of replication and recombination in the replicase molecule supports the model that RNA recombination represents a distinct function of this protein, although the underlying mechanism still needs to be investigated.

Potato virus X amplicon-mediated silencing of nuclear genes

1999 ◽  
Vol 20 (3) ◽  
pp. 357-362 ◽  
Author(s):  
Susan M. Angell ◽  
David C. Baulcombe
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Nuclear Genes

Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs

Acta Naturae ◽  
2011 ◽  
Vol 3 (3) ◽  
pp. 40-46 ◽  
Author(s):  
M V Arkhipenko ◽  
E K Petrova ◽  
N A Nikitin ◽  
A D Protopopova ◽  
E V Dubrovin ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus X ◽  
Viral Rnas ◽  
Virus Like Particles ◽  
Artificial Virus

STUDY ОF SOME MOLECULAR-GENETIK PROPERTIES OF THE POTATO VIRUS X

2020 ◽  
Vol 4 (2) ◽  
pp. 4-11
Author(s):  
Vokhid Fayziev ◽  
◽  
Dilfuza Javlieva ◽  
Umida Jurayeva ◽  
Zarifa Kadirova ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus X
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