A GROWTH INHIBITOR FROM THE ROOTS OF CHRYSANTHEMUM MORIFOLIUM RAMAT. AND ITS INFLUENCE UPON THE GIBBERELLIC ACID RESPONSE

1962 ◽  
Vol 40 (9) ◽  
pp. 1229-1235 ◽  
Author(s):  
D. J. Ballantyne
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Growth Inhibitor ◽  
Chrysanthemum Morifolium ◽  
Naphthalene Acetic Acid ◽  
Root Extract ◽  
Wheat Coleoptile ◽  
Flower Buds ◽  
Inhibiting Factor ◽  
Chrysanthemum Morifolium Ramat

The growth-reducing factor in old chrysanthemum soil previously reported in Ontario was found to inhibit growth by reducing number of internodes and internode length. A further effect was the reduction in number of flower buds. Gibberellic acid applications could offset growth reductions but enhanced reduction in floral number. Factors that could inhibit growth of wheat coleoptile sections could be extracted from chrysanthemum roots and buds. A methanolic root extract, after being subjected to paper chromatography with 80% isopropanol, slightly inhibited the growth of chrysanthemums. This inhibiting factor was in the same region of the chromatogram as the factor which inhibited the growth of wheat coleoptile sections. The response to the growth reducing factor is compared with that to naphthalene acetic acid.

FTIR, GC-MS and HPLC analysis of Baliospermum montanum (Willd.) Muell. Arg.

2020 ◽  
Vol 11 (4) ◽  
pp. 5059-5066
Author(s):  
Sushma B K ◽  
Raveesha H R
Keyword(s):  
Acetic Acid ◽  
Quantitative Estimation ◽  
Chemical Constituents ◽  
Standard Procedure ◽  
Naphthalene Acetic Acid ◽  
Root Extract ◽  
Pentanoic Acid ◽  
Trimethylsilyl Ester ◽  
Isolation And Characterization ◽  
Ms Analysis

The present work is aimed to determine the chemical constituents in Baliospermum montanum methanolic extracts. An in vitro regenerated procedure was developed for the induction of callus from stem explant cultured on Murashige and Skoog (MS) medium fortified with various concentration and permutations of 2, 4-dichloro phenoxy acetic acid, 1-naphthalene acetic acid, 6-benzyl amino purine and gibberellic acid. FTIR & GC-MS analysis was done according to standard procedure. The quantitative estimation of β-sitosterol was done by HPLC method. Maximum fresh and dry weight of callus was estimated in the combination of GA3 (0.5 mg/L) + NAA (2 mg/L) compared to other concentration. The FTIR analysis showed various functional compounds with different characteristic peak values in the extracts. Major bioactive constituents were recognized in the GC-MS analysis. Root extract revealed the existence of 1-hexadecanol, pentanoic acid, 2-(aminooxy)- and 1-hexacosanol. Leaf extract showed the presence of propanoic acid, 2-oxo-, trimethylsilyl ester, 9,12-octadecadienoic acid (z,z)-, trimethylsilyl ester, docosane, 1,22-dibromo- and pentatriacontane. Stem and stem derived callus exhibit the presence of 1,6,3,4-dihydro-2-deoxy-beta-d-lyxo-hexopyranose, n-hexadecanoic acid and pentanoic acid, 2-(aminooxy). The methanolic extract of leaf exhibited 0.2149 % of β-sitosterol content. There were no peaks observed in the root, stem and stem derived callus. Further studies are necessary for the isolation and characterization of bioactive compounds from B. montanum.

scholarly journals Effect of chemical thinning, gibberellic acid and pruning on growth and production of nectarine (Prunus persica (L.) Batsch var. nucipersica) cv. May fire

2017 ◽  
Vol 9 (1) ◽  
pp. 332-337
Author(s):  
Rimpika Rimpika ◽  
N. Sharma ◽  
D.P. Sharma
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Shoot Growth ◽  
Prunus Persica ◽  
Tree Height ◽  
Naphthalene Acetic Acid ◽  
Crop Load ◽  
Fruit Drop ◽  
Alternative Approach ◽  
Old Trees

The present investigation was carried out on 12-year old trees of nectarine (Prunus persica (L.) Batsch var. nucipersica) cultivar May Fire raised on wild peach seedling rootstocks, Farmer’s orchard at Kotla- Barog in District Sirmour during the years 2014 and 2015. In this experiment, experimental trees were subjected to seventeen different treatments;T1: Pruning to retain 60 fruiting shoots tree-1 (control), T2: Pruning to retain 50 fruiting shoots tree-1, T3: Pruning to retain 40 fruiting shoots tree-1 ,T4: NAA 40 ppm, 2 WAPF (week after petal fall) ,T5: Ethrel 300 ppm, 2 WAPF, T6: GA3 100 ppm, 4 WAPF, T7: GA3 200 ppm, 4WAPF, T8: GA3 100 ppm, 6 WAPF T9: GA3 200 ppm, 6 WAPF, T10: Pruning to retain 50 fruiting shoots tree-1 + NAA 40 ppm (T4), T11: Pruning to retain 50 fruiting shoots tree-1 + Ethrel 300ppm (T5), T12: Pruning to retain 50 fruiting shoots tree-1 + GA3100 ppm (T6), T13: Pruning to retain 50 fruiting shoots tree-1 + GA3 100 ppm (T8),T14: Pruning to retain 40 fruiting shoots tree-1 + NAA 40 ppm (T4), T15: Pruning to retain 40 fruiting shoots tree -1 +Ethrel 300 ppm (T5),T16: Pruning to retain 40 fruiting shoots tree -1 + GA3 100 ppm (T6), T17: Pruning to retain 40 fruiting shoots tree -1 + GA3 100 ppm (T8), pertaining to alternative approach to chemical thinning, chemical thinner were more effective in improving the production of superior grade fruits than reducing the crop load directly by pruning or indirectly by GA 3 (Gibberellic Acid) treatments. Treatments with NAA (naphthalene acetic acid) at 40 ppm when applied two weeks after petal fall reduced the crop load to the greatest extent and improve the yield of superior grade fruits and increase the leaf to fruit ratio and decrease the fruit drop. Pruning to retain 40 fruiting shoots tree-1 + NAA 40 ppm, 2WAPF increased the shoot growth (160.80, 170.20cm), tree height (3.70,4.50 cm), tree spread (2.84,3.60), leaf area (42.71, 40.63 cm) during both the year. However, effect of chemical were less pronounced on trees subjected to severe pruning.

scholarly journals In vitro propagation of the endangered orchid Dendrobium chryseum Rolfefrom protocorms culture

2020 ◽  
Vol 19 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Sabitri Maharjan ◽  
Laxmi Sen Thakuri ◽  
Bir Bahadur Thapa ◽  
Shreeti Pradhan ◽  
Krishna K. Pant ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Mass Propagation ◽  
Endangered Orchid ◽  
Immature Seeds ◽  
Indole 3 Acetic Acid

 The immature seeds of Dendrobiumchryseum, asympodial epiphytic orchid with yellow flowers, were cultured in vitro, and the resultant protocorms were used as explants for seedling development. Protocorms were cultured on½ M.S. medium fortified with Kinetin (Kn), 6-Benzylaminopurine (BAP), and Gibberellic Acid (GA3) in three concentrations (0.5mg/l, 1.0mg/land 2.0 mg/l) both alone and supplemented with 5% and 10% coconut water (C.W.). The highest number of shootsofD. chryseum developed on ½ - M.S. medium fortified with 2.0mg/lofKn and10% C.W. and the longest shootsdeveloped on ½ M.S. media fortified with 1.0mg/lGA3, and 10% C.W. The shoot derived from protocorms were placed in ½ M.S. medium fortified with three different rooting hormones, Indole -3- acetic acid (IAA), Indole -3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA) in different concentrations alone as well as with each 1.0mg/l hormone combined with 10% C.W. The most effective of these media was ½ M.S. medium fortified with 1.5 mg/l IAA for rooting as well as for the production of longest roots. The present study could be useful for standardizing the protocol for mass propagation of the endangered orchid Dendrobiumchryseum.

scholarly journals Some Properties of a Plant Growth Inhibitor Present in Xylem Sap of Woody Species

1965 ◽  
Vol 18 (3) ◽  
pp. 475 ◽  
Author(s):  
RM Da Vison
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Sucrose Solution ◽  
Xylem Sap ◽  
Woody Species ◽  
Growth Inhibitor ◽  
Inhibitory Effect ◽  
Wheat Coleoptile ◽  
Coleoptile Section ◽  
Coleoptile Elongation

An ether-soluble, non-toxic inhibitor of plant growth was shown to be present in varying amounts in xylem sap from shoots of various woody species. Some chromatographic properties of the inhibitor are described. The inhibitor reduced growth of wheat coleoptile sections and lettuce hypocotyls, and inhibited germination of lett,uce and cress seeds. The inhibitor did not interact competitively with either auxin in the wheat coleoptile elongation test or with gibberellic acid in the lettuce hypocotyl test. Gibberellic acid did not overcome the inhibitory effect on seed germination. The inhibition of coleoptile section growth was reversed on transfer from inhibitor solution to sucrose solution.

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