PHOTOSYNTHESIS AND METABOLISM OF MARINE ALGAE: I. PHOTOSYNTHESIS OF TWO MARINE FLAGELLATES COMPARED WITH CHLORELLA

1957 ◽  
Vol 35 (6) ◽  
pp. 945-950 ◽  
Author(s):  
R. G. S. Bidwell
Keyword(s):  
Amino Acids ◽  
Acid Hydrolysis ◽  
Marine Algae ◽  
Chlorella Pyrenoidosa ◽  
Soluble Product ◽  
Fixed Carbon ◽  
Radioactive Carbon ◽  
Chromatographic Techniques

The products of photosynthesis of two marine flagellates have been examined and compared with those of Chlorella pyrenoidosa with the use of radioactive carbon and chromatographic techniques. The alcohol-soluble products were different in each alga, many of the compounds produced by the marine forms being unidentified. The principal soluble product of photosynthesis of a member of the Heterokontae, Olisthodiscus sp., was identified as d-mannitol. Those of a dinoflagellate, Amphidinium carteri, include glycosides of glucose and maunitol. The main insoluble carbohydrate produced by all three organisms gave rise to glucose on hydrolysis; this only contained about ten per cent of the total fixed carbon. The radioactive compounds released from the alcohol-insoluble residues by acid hydrolysis were similar in the three algae and consisted mainly of amino acids.

D-Mannitol Interferes with Amino Acid Analysis of Marine Organisms

1979 ◽  
Vol 36 (9) ◽  
pp. 1134-1137 ◽  
Author(s):  
W. Fong ◽  
R. K. O'dor
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Hydrolysis ◽  
Key Words ◽  
Amino Acid Analysis ◽  
Chromatographic Analysis ◽  
Marine Algae ◽  
Protein S ◽  
New Compounds ◽  
Hydrolysis Of

Acid hydrolysis of a protein in the presence of D-mannitol, a common constituent of marine algae, can cause significant reductions in the recovery of a number of amino acids. The new compounds formed by the interactions of D-mannitol and these amino acids may interfere in the chromatographic analysis of other amino acids. The recoveries of most of the amino acids appear to be either directly or inversely proportional to the amount of D-mannitol added to a protein sample before acid hydrolysis. These results suggest that it is necessary to determine the effects of contaminants in a sample of protein(s) on the recoveries of amino acids during routine acid hydrolysis. Key words: kelp, amino acids, carbohydrates, D-mannitol

Optimisation of microwave-assisted acid hydrolysis for the determination of seleno-amino acids bound to proteins in powdered milk, lyophilized milk and infant formula

2019 ◽  
Vol 79 ◽  
pp. 128-133
Author(s):  
Romina Lopez ◽  
Luis Escudero ◽  
Roberto D’Amato ◽  
Daniela Businelli ◽  
Massimo Trabalza-Marinucci ◽  
...  
Keyword(s):  
Amino Acids ◽  
Acid Hydrolysis ◽  
Infant Formula ◽  
Powdered Milk ◽  
Microwave Assisted

CHEMICAL COMPOSITION OF HYPHAL AND SCLEROTIAL WALLS OF SCLEROTIUM ROLFSII SACC.

1967 ◽  
Vol 13 (2) ◽  
pp. 137-141 ◽  
Author(s):  
I. Chet ◽  
Y. Henis ◽  
R. Mitchell
Keyword(s):  
Amino Acids ◽  
Chemical Composition ◽  
Acid Hydrolysis ◽  
Sclerotium Rolfsii ◽  
Ash Content ◽  
Chemical Degradation ◽  
Chemical Components

The chemical components of the sclerotial and hyphal walls of the fungus Sclerotium rolfsii Sacc. are compared. Sclerotial walls contain a melanin-like pigment which is absent from hyphal walls. They are also higher in non-hydro-lyzable residue, in lipids, and in ash content. In both walls, glucose, mannose, and glucosamine are found after acid hydrolysis. Acid hydrolysates of sclerotial and hyphal walls contain 10 and 13 amino acids, respectively, L-arginine, L-serine, and L-proline being absent from sclerotial walls. The possible role of some of these components in the resistance of the sclerotia to biological and chemical degradation is discussed.

COMPETITION BETWEEN AMINO ACIDS FOR TRANSPORT INTO EHRLICH ASCITES CARCINOMA CELLS

1961 ◽  
Vol 39 (11) ◽  
pp. 1717-1735 ◽  
Author(s):  
P. G. Scholefield
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids ◽  
Amino Acid ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Transport Systems ◽  
Radioactive Carbon ◽  
Ehrlich Ascites ◽  
Competitive Inhibitors ◽  
Amino Acid Analogues

The cumulative entry of amino acids into Ehrlich ascites carcinoma cells is due to the presence of active transport systems, each with its own specific range of substrates. Several amino acids and amino acid analogues may have an affinity for the same transport system and thus may inhibit transport of other amino acids by acting as competitive inhibitors or competitive substrates. Loss of methionine from ascites cells takes place by a diffusion process which obeys Fick's law. Leucine accumulation by ascites cells is small and is increased on addition of certain other amino acids. The increase is not due to inhibition of leucine oxidation as increase in the rate of production of radioactive carbon dioxide from labeled leucine also occurs. Kinetic aspects of these results are discussed.

The influence of environmental features in the content of mycosporine-like amino acids in red marine algae along the Brazilian coast

2018 ◽  
Vol 54 (3) ◽  
pp. 380-390 ◽  
Author(s):  
Bruno Briani ◽  
Marina N. Sissini ◽  
Leidson A. Lucena ◽  
Manuela B. Batista ◽  
Iara O. Costa ◽  
...  
Keyword(s):  
Amino Acids ◽  
Marine Algae ◽  
Brazilian Coast ◽  
Environmental Features

Gas Chromatography in Diagnostic Biochemistry of Abnormal Valine Metabolism

1973 ◽  
Vol 19 (9) ◽  
pp. 1040-1044 ◽  
Author(s):  
Hugh N Tucker ◽  
Samuel V Molinary
Keyword(s):  
Amino Acids ◽  
Gas Chromatography ◽  
Amino Acid ◽  
Biological Fluids ◽  
Sample Sizes ◽  
Diagnostic Aid ◽  
Chromatographic Techniques ◽  
Biochemical Evaluation ◽  
Amino Acid Concentrations ◽  
Chromatographic Evidence

Abstract Amino acids in biological fluids can be rapidly and accurately quantitated by gas chromatography. Modification of existing gas-chromatographic techniques allow as little as 25 µl of biological fluids to be analyzed, compatible with sample sizes easily obtained in pediatric studies. Simplified diagnostic biochemical evaluation of a suspected aminoaciduria can be begun at birth, or shortly after. Normal amino acid patterns are compared with those found in a patient with abnormal amino acid concentrations. Gas chromatographic evidence is presented as a diagnostic aid in the evaluation of a patient with abnormal valine metabolism.

THE INFLUENCE OF NITROGENOUS COMPOUNDS ON GROWTH OF PYTHIUM SPECIES

1967 ◽  
Vol 13 (11) ◽  
pp. 1509-1519 ◽  
Author(s):  
V. P. Agnihotri ◽  
O. Vaartaja
Keyword(s):  
Amino Acids ◽  
Organic Nitrogen ◽  
Aminobutyric Acid ◽  
Pythium Species ◽  
Surface Culture ◽  
Poor Growth ◽  
Nitrogenous Compounds ◽  
Chromatographic Techniques ◽  
Ammonium Compounds ◽  
N Compounds

The utilization of N compounds by P. ultimum Trow (strain I and II), P. rostratum Butler, and P. irregulare Buisman was examined in a chemically denned medium under controlled conditions in surface culture. All species were able to metabolize nitrate, ammonium, and organic nitrogen, and the amount of growth varied with the nitrogen source. In general, yeast extract, peptone, glycine, serine, histidine, cysteine, asparagine, aspartic acid, and glutamic acid supported favorable growth, whereas γ-aminobutyric acid, threonine, and alanine supported poor growth of these fungi. The addition of succinic acid at 0.02 M concentration to ammonium compounds further increased growth of four isolates.Preferential utilization of amino acids from a given mixture was recorded using paper chromatographic techniques. All four isolates gave more vegetative growth on mixtures of amino acids than when they were supplied singly.

Amino Acids from Marine Algae

1980 ◽  
pp. 95-140 ◽  
Author(s):  
ERNESTO FATTORUSSO ◽  
MARIO PIATTELLI
Keyword(s):  
Amino Acids ◽  
Marine Algae
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