ON THE EXTRACTION OF ENZYMES FROM SUCCULENT PLANTS

1957 ◽  
Vol 35 (1) ◽  
pp. 25-30 ◽  
Author(s):  
C. H. Coles ◽  
E. R. Waygood

The extraction of enzymes concerned with malic acid metabolism from succulent plants, notably Bryophyllum calycinum, has been made possible by the introduction of a new technique. The high concentration of organic acids in the vacuoles of leaf cells was neutralized in situ by vacuum infiltration with ammonia solution. Thus, during maceration, the enzyme proteins are neither precipitated nor inactivated and conventional methods of enzyme purification may be applied. Further experiments have shown that whereas B. calycinum plants may lose their ability to fix carbon dioxide actively in the dark following exposure to short days ([Formula: see text] hours) the enzymes involved in malic acid metabolism and carbon dioxide fixation in the dark are not necessarily depleted.

2021 ◽  
pp. 51413
Author(s):  
Rong Yang ◽  
Hongwei Cao ◽  
Chong Li ◽  
Guoxiang Zou ◽  
Xin Zhang ◽  
...  

2021 ◽  
Author(s):  
Ramón Casillas‐Hernández ◽  
Karla Janeth Arévalo‐Sainz ◽  
Jose Reyes Gonzalez‐Galaviz ◽  
María del Carmen Rodríguez‐Jaramillo ◽  
Rafael Apolinar Bórquez‐López ◽  
...  

2021 ◽  
Vol 13 (8) ◽  
pp. 4591
Author(s):  
Shuanglei Huang ◽  
Daishe Wu

The tremendous input of ammonium and rare earth element (REE) ions released by the enormous consumption of (NH4)2SO4 in in situ leaching for ion-adsorption RE mining caused serious ground and surface water contamination. Anaerobic ammonium oxidation (anammox) was a sustainable in situ technology that can reduce this nitrogen pollution. In this research, in situ, semi in situ, and ex situ method of inoculation that included low-concentration (0.02 mg·L−1) and high-concentration (0.10 mg·L−1) lanthanum (La)(III) were adopted to explore effective start-up strategies for starting up anammox reactors seeded with activated sludge and anammox sludge. The reactors were refrigerated for 30 days at 4 °C to investigate the effects of La(III) during a period of low-temperature. The results showed that the in situ and semi in situ enrichment strategies with the addition of La(III) at a low-concentration La(III) addition (0.02 mg·L−1) reduced the length of time required to reactivate the sludge until it reached a state of stable anammox activity and high nitrogen removal efficiency by 60–71 days. The addition of La(III) promoted the formation of sludge floc with a compact structure that enabled it to resist the adverse effects of low temperature and so to maintain a high abundance of AnAOB and microbacterial community diversity of sludge during refrigeration period. The addition of La(III) at a high concentration caused the cellular percentage of AnAOB to decrease from 54.60 ± 6.19% to 17.35 ± 6.69% during the enrichment and reduced nitrogen removal efficiency to an unrecoverable level to post-refrigeration.


2007 ◽  
Vol 22 (2) ◽  
pp. 428-436 ◽  
Author(s):  
S. Jayalakshmi ◽  
J.P. Ahn ◽  
K.B. Kim ◽  
E. Fleury

We report the hydrogenation characteristics and mechanical properties of Ti50Zr25Cu25 in situ composite ribbons, composed of β-Ti crystalline phase dispersed in an amorphous matrix. Upon cathodic charging at room temperature, high hydrogen absorption up to ∼60 at.% (H/M = ∼1.2) is obtained. At such a high concentration, hydrogen-induced amorphization occurs. Mechanical tests conducted on the composite with varying hydrogen concentrations indicate that the Ti50Zr25Cu25 alloy is significantly resistant to hydrogen embrittlement when compared to conventional amorphous alloys. A possible mechanism that would contribute toward hydrogen-induced amorphization and hydrogen embrittlement is discussed.


2007 ◽  
Vol 18 (8) ◽  
pp. 969-972
Author(s):  
Zhao Yang Wang ◽  
Huan Feng Jiang ◽  
Chao Rong Qi ◽  
Yan Xia Shen ◽  
Shao Rong Yang

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