SEDIMENTARY CHLOROPHYLL DETERMINATION AS A PALEOBOTANICAL METHOD

1955 ◽  
Vol 33 (4) ◽  
pp. 304-313 ◽  
Author(s):  
J. R. Vallentyne
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Light Absorption ◽  
Quantitative Method ◽  
Degradation Products ◽  
Wave Length ◽  
Chlorophyll Degradation ◽  
Plant Tissues ◽  
Sediment Profiles

Chlorophyll degradation products which can be extracted from sediments with 90% acetone and which show maximal light absorption in the red end of the spectrum at a wave length of 667 mμ (90% acetone as the solvent) are here collectively defined as sedimentary chlorophyll. A quantitative method is described for the determination of sedimentary chlorophyll. Results from six different sediment profiles are presented. The nature of sedimentary chlorophyll is unknown, except that it consists of at least three chlorophyll degradation products. Sedimentary chlorophyll has been found in samples aged up to 11,000 years. In any one profile the fluctuations in sedimentary chlorophyll content may be used to infer changes in the abundance of chlorophyll-bearing plant tissues, but only with the greatest caution.

scholarly journals Determination of Chlorophyll Degradation Products in Chlorophyll Color Preparations

1997 ◽  
Vol 38 (3) ◽  
pp. 155-160_1 ◽  
Author(s):  
Keiko HIRATA ◽  
Masako HIROKADO ◽  
Yoko UEMATSU ◽  
Yuki SADAMASU ◽  
Kazuo YASUDA ◽  
...  
Keyword(s):  
Degradation Products ◽  
Chlorophyll Degradation

scholarly journals ANALISIS KANDUNGAN KLOROFIL PADA BERBAGAI POSISI DAUN DAN ANAK DAUN AREN (Arrenga pinnata)

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Olivia P. G. Lawendatu ◽  
Julius Pontoh ◽  
Vanda Kamu
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Palm Tree ◽  
Plant Canopy ◽  
Chlorophyll B ◽  
Chlorophyll A And B ◽  
Palm Leaf

ABSTRAKTelah dilakukan penelitian tentang analisis kandungan klorofil dari berbagai posisi daun dan anak daun dari tanaman aren. Daun aren diambil pada posisi atas, tengah, dan bawah dari tajuk tanaman. Sampel anak daun  dibagi 3 posisi yaitu atas, tengah, dan bawah. Ekstraksi dilakukan dengan methanol dan penetuan klorofil pada daun dilakukan dengan spektofotometer pada panjang gelombang 665 nm dan 652 nm untuk masing-masing klorofil a dan klorofil b. Kandungan klorofil a dan b  yang terdapat pada sampel daun aren yang dihitung berdasarkan 3 unit satuan µg/mL, mg/g  dan µmol/m2. Kandungan klorofil a dan b pada sampel pohon aren tertinggi terletak pada posisi kiri daun, pada posisi daun terletak pada daun atas, pada posisi anak daun terletak pada posisi ujung anak daun. Ratio kandungan klorofil pada sampel daun aren berkisar 1,30-6,72. ABSTRACTHas conducted research on the analysis of chlorophyll content from various positions of leaves and leaves of palm plants. Leaves are taken in the top, middle and bottom positions of the plant canopy. Samples of leaflets were divided into 3 positions namely top, middle and bottom. Extraction was carried out with methanol and the determination of chlorophyll in the leaves was carried out by spectrophotometer at wavelengths of 665 nm and 652 nm for chlorophyll a and chlorophyll b, respectively. The chlorophyll and b content in palm leaf samples are calculated based on 3 units of μg/mL, mg/g, and µmol/m2. The content of chlorophyll a and b in the highest palm tree sample is located in the left position of the leaf, the position of the leaf is placed on the top of the leaf, the position of the leaflets is placed in the tip of the leaflets. The ratio of chlorophyll content in sugar palm leaf samples in the setting is 1.30-6.72. 

scholarly journals Revisiting chlorophyll extraction methods in biological soil crusts – methodology for determination of chlorophyll a and chlorophyll a + b as compared to previous methods

2017 ◽  
Author(s):  
Jennifer Caesar ◽  
Alexandra Tamm ◽  
Nina Ruckteschler ◽  
Bettina Weber
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Extraction Method ◽  
Extraction Procedure ◽  
Extraction Methods ◽  
Intermediate Step ◽  
Green Algal ◽  
Chlorophyll Extraction ◽  
The Impact

Abstract. Chlorophyll concentrations of biological soil crust (biocrust) samples are commonly determined to quantify the relevance of photosynthetically active organisms within these surface soil communities. Whereas chlorophyll extraction methods for freshwater algae and leaf tissues of vascular plants are well established, there is still some uncertainty regarding the optimal extraction method for biocrusts, where organism composition is highly variable and samples comprise major amounts of soil. In this study we analyzed the efficiency of two different chlorophyll extraction solvents, the effect of grinding the soil samples prior to the extraction procedure and the impact of shaking as an intermediate step during extraction. The analyses were conducted on four different types of biocrusts. Our results show, that for all biocrust types chlorophyll contents obtained with ethanol were significantly lower than those obtained with dimethyl sulfoxide (DMSO) as solvent. Grinding of biocrust samples prior to analysis caused a highly significant decrease in chlorophyll content for green algal lichen- and cyanolichen-dominated biocrusts, and a tendency towards lower values for moss- and algae-dominated biocrusts. Shaking of the samples after each extraction step had a significant positive effect on the chlorophyll content of green algal lichen- and cyanolichen-dominated biocrusts. Based on our results we confirm a DMSO-based chlorophyll extraction method without grinding pretreatment and suggest to insert an intermediate shaking step for complete chlorophyll extraction (see supplement S6 for detailed manual). Determination of a universal chlorophyll extraction method for biocrusts is essential for the inter-comparability of studies conducted across all continents.

Is Quantitative Determination of Fibrin(ogen) Degradation Products and Thrombin-Antithrombin III Complexes Useful to Diagnose Deep Venous Thrombosis in Outpatients?

1989 ◽  
Vol 62 (04) ◽  
pp. 1043-1045 ◽  
Author(s):  
Paul F M M van Bergen ◽  
Eduard A R Knot ◽  
Jan J C Jonker ◽  
Auke C de Boer ◽  
Moniek P M de Maat
Keyword(s):  
Quantitative Determination ◽  
Venous Thrombosis ◽  
Deep Venous Thrombosis ◽  
Antithrombin Iii ◽  
Degradation Products ◽  
Family Doctor ◽  
Diagnostic Value ◽  
Impedance Plethysmography ◽  
Fibrin Degradation Products

SummaryWe studied the diagnostic value of recently introduced ELISA’s for the determination of thrombin-antithrombin III (TAT) complexes, fibrin degradation products (FbDP), fibrinogen degradation products (FgDP) and total degradation products (TDP) for deep venous thrombosis (DVT) in plasma of 239 consecutive outpatients, suspected for DVT by their family doctor. DVT was confirmed by impedance plethysmography in 60 patients. Using the 95th percentile range of 42 healthy volunteers the sensitivity for the detection of DVT was: 37% for TAT, 95% for TDP, 92% for FbDP and 90% for FgDP. Specificity was: 88% for TAT, 16% for TDP, 20% for FbDP and 25% for FgDP.We conclude that these assays are of little value in the diagnosis of DVT in outpatients.

Estimation of chlorophyll content by absorption spectra of native Spirulina platensis cells

2020 ◽  
pp. 25-33
Author(s):  
Leonid E. Paramonov
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Absorption Spectra ◽  
Spirulina Platensis ◽  
Intracellular Concentration ◽  
Absorption Coefficients

A method for retrieving the absorption coefficients of Spirulina platensis pigments using absorption spectra of native cells and excluding the use of extracts is considered. Estimates of the intracellular concentration of chlorophyll a, С-phycoerythrin, С- phycocyanin and allophycocyanin in native cells are discussed.

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