STUDIES ON THE FORMATION OF VITAMIN C IN SLICES OF POTATO TISSUE

1952 ◽  
Vol 30 (6) ◽  
pp. 665-673 ◽  
Author(s):  
E. A. M. Asselbergs ◽  
F. J. Francis
Keyword(s):  
Vitamin C ◽  
Direct Relationship ◽  
Indoleacetic Acid ◽  
Physiological Condition ◽  
Potassium Cyanide ◽  
Naphthaleneacetic Acid ◽  
Distilled Water ◽  
Potato Tissue ◽  
Enzyme Systems ◽  
Potato Slices

When slices of potato tissue are stored for two days at 23 °C. in moist chambers or in aerated distilled water, the vitamin C content increases 100-300% as measured by the method of Roe et al. The increase does not occur in slices from freshly harvested potatoes. The physiological condition of the potatoes at the time of the experiment is important in determining the amount of increase in slices from potatoes stored for several months. Experiments with auxins and mannitol solutions show that there is no direct relationship between the process of water uptake and vitamin C formation. Solutions of indoleacetic acid and naphthaleneacetic acid increase the amount of vitamin formed. The results of experiments with potassium cyanide, sodium azide, malachite green, 8-hydroxyquinoline, sodium diethyldithiocarbamate and 2,4-dinitrophenol suggest that the formation of vitamin C is not directly related to any of the enzyme systems inhibited by the above chemicals. Infiltration of potato slices with solutions of fumaric and malic acid results in an increased formation of vitamin C as compared with the water controls.

Root and Shoot Development on Cuttings of Basswood (Tiliaamericana L.) as Affected by Auxin Treatments and Size of Cuttings

1974 ◽  
Vol 4 (2) ◽  
pp. 246-249 ◽  
Author(s):  
W. A. G. Morsink ◽  
V. G. Smith
Keyword(s):  
Aqueous Solution ◽  
Root Growth ◽  
Aqueous Solutions ◽  
Indoleacetic Acid ◽  
Shoot Development ◽  
Naphthaleneacetic Acid ◽  
Distilled Water ◽  
Water Control ◽  
Indolebutyric Acid

A population of saplings of basswood was studied to determine the effect of size of cutting and a 2-min basal dip in an aqueous solution of either indolebutyric acid (IBA), indoleacetic acid (IAA), or naphthaleneacetic acid (NAA) on root and shoot development of softwood cuttings grown under intermittent mist. Cuttings were 12–60 cm long, and treatments were: distilled water control; and aqueous solutions of auxin at levels of 1000, 5000, 10 000, and 20 000 p.p.m. Significant increases in the growth of roots were obtained with 5000 and 10 000 p.p.m. of IBA. Significant increases in root growth were also obtained with increasing sizes of cuttings.

scholarly journals Optimization of the blanching of potato (Solanum tuberosum L) slices by response surface methodology: Effect on the vitamin C content and drying kinetics

2020 ◽  
pp. 17-32
Author(s):  
Ambe Desmond ◽  
Lobe Elias ◽  
Divine B. Nde
Keyword(s):  
Vitamin C ◽  
Solanum Tuberosum L ◽  
Negative Influence ◽  
Drying Kinetics ◽  
Slice Thickness ◽  
Drying Time ◽  
Quality Marker ◽  
Thin Layer Drying ◽  
Drying Behavior ◽  
Potato Slices

Potatoes (S. tuberosum L) is one of the most important tuber crops in the world. However, its high moisture content and inadequate storage and processing techniques have a negative influence on its quality and availability throughout the year. This work was carried out to optimize the blanching behavior and drying kinetics of potato slices. Fresh potato slices (5, 10 and 15 mm) were blanched at 70, 80 and 90°C for 1, 3 and 5 min following a 3k level full factorial design. The loss in Vitamin C was used as a quality marker for the optimization process. The influence of blanching on the drying behavior of potato slices was carried out at 50, 60 and 70°C. Results showed that blanching parameters had a significant (P < 0.05) effect on vitamin C loss. Optimum blanching conditions were blanching temperature of 80oC, blanching time of 3 min and a slice thickness of 10 mm which gave an average loss in Vitamin C of about 2.6%. Drying data were successfully fitted to three different thin layer drying models. The use of blanching as a pretreatment before the drying of potato is recommended because it reduces the drying time by 30%.

scholarly journals Evaluation of Male Fertility-Enhancing Activities of Water Seed Extract of Hunteria umbellata in Wistar Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Adejuwon Adewale Adeneye ◽  
Joseph Abayomi Olagunju ◽  
Babatunde Adekunle Murtala
Keyword(s):  
Weight Gain ◽  
Vitamin C ◽  
Male Fertility ◽  
Wistar Rats ◽  
Oral Treatment ◽  
Seed Extract ◽  
Distilled Water ◽  
Hormonal Profile ◽  
Group I ◽  
Dose Dependent

Background. In this study, the male fertility-enhancing activity of 100, 200, and 400 mg/kg/day of Hunteria umbellata water seed extract (HU) in Wistar rats was studied for 60 days. In doing this, effect of repeated doses of HU was studied on the weight gain pattern, gonadosomatic index (GSI), serum follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (TS), prolactin (PRL), and estradiol (ES)} as well as testicular antioxidant status of the treated rats as a way of elucidating the mechanism(s) of action of HU. Method. Thirty-six (36) male Wistar rats were randomly divided into six groups (I-VI) of six rats per group. Group I rats were gavaged with 10 ml/kg/day of distilled water and served as an untreated control; Group II rats were gavaged with 0.3 mg/kg/day of clomiphene in distilled water; Groups III-V rats received 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day of HU, respectively, and Group VI rats received 20 mg/kg/day of Vitamin C all in distilled water. All treatments were for 60 days after which the treated rats were humanely sacrificed. Sera of blood samples were processed for the above stated hormonal profile. Similarly, testicular tissues obtained were processed for semen analysis and complete antioxidant profile of the HU-treated testicles by assaying for superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH), glutathione reductase (GSR), glutathione peroxidase (GSH-Px), and Thiobarbituric Reactive Species (TBARS). Results. Prolonged treatments with 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day of HU for 60 days induced dose dependent reductions in weight gain pattern with the most significant (p<0.001) effect recorded with the highest dose of HU. Conversely, significant (p<0.001) increase was recorded for GSI at the same HU dose. Clomiphene and HU also induced significant (p<0.01, p<0.001) dose dependent increases in the total sperm count, %live sperm, but reverse effects on %dead sperm and %abnormal sperm. On the hormonal profile, oral treatment with 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day of the extract induced profound (p<0.05, p<0.01, and p<0.001) dose related increases in the sera TS, LH, and FSH while it caused reverse effect on serum PRL but caused no significant alterations in the serum ES levels. Similarly, oral treatment with vitamin C and 100-400 mg/kg/day of HU induced profound (p<0.05, p<0.01, and p<0.001) increases in the antioxidant enzyme activities. Conclusion. Overall, prolonged oral treatment with 100-400 mg/kg body weight of HU for 60 days significantly improved sperm function which was mediated via enhanced spermatogenesis, steroidogenesis, and antioxidant mechanisms.

In vitro propagation of Crambe maritima

1987 ◽  
Vol 65 (1) ◽  
pp. 72-75 ◽  
Author(s):  
J. Y. Peron ◽  
E. Regnier
Keyword(s):  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Petiole Explants ◽  
Acclimatization Period

A method for rapid micropropagation of sea kale (Crambe maritima L.) was developed. Petiole explants placed in vitro on a medium containing 0.5 mg/L indoleacetic acid (IAA), 6.0 mg/L kinetin, and 1.5 mg/L benzylaminopurine developed callus within 15 days and shoots within 28 days. Nearly four adventitious shoots could be developed within 3 weeks by placing the initial shoot on media without IAA. To develop roots, the shoots were then transferred to the basal medium containing 0.1 to 1.0 mg/L indolbutyric or α-naphthaleneacetic acid. Rooted plantlets were obtained within 2 or 3 weeks. After an acclimatization period of 6 weeks in a greenhouse in unsterilized medium, the plantlets could be set outdoors.

Observations on the decrease in sloughing of potato tissue slices on soaking in distilled water

1963 ◽  
Vol 40 (10) ◽  
pp. 333-343 ◽  
Author(s):  
Mary V. Zaehringer ◽  
Helen H. Cunningham ◽  
Duane J. LeTourneau
Keyword(s):  
Distilled Water ◽  
Potato Tissue ◽  
Tissue Slices

CORRELATIONS AFFECTING REGENERATION AND REACTIVATION IN SPLACHNUM AMPULLACEUM (L.) HEDW.

1959 ◽  
Vol 37 (1) ◽  
pp. 121-134 ◽  
Author(s):  
Ian G. MacQuarrie ◽  
Kraft E. von Maltzahn
Keyword(s):  
Apical Dominance ◽  
Direct Relationship ◽  
Indoleacetic Acid ◽  
Leaf Size ◽  
Primary Factor ◽  
Reversible Inhibition ◽  
Correlative Inhibition ◽  
Stems And Leaves ◽  
Phenylbutyric Acid ◽  
Stem Segments

Correlations affecting restitutional behavior in the gametophore are examined. In short stem segments a direct relationship is found between the length of the segment and the number of regenerates; in longer segments this relationship disappears. No such correlation exists between leaf size and number of regenerates per leaf. The presence of leaves increases regeneration from the stem; the stem inhibits regeneration from the leaf.Isolation and not wounding is shown to be the most important factor in leaf regeneration.The apex of the gametophore inhibits both regeneration from the base of the gametophore and bud reactivation. These apical dominance effects can be replaced by the application of indoleacetic acid (IAA) to the tips of decapitated plants. Similar concentrations of IAA and γ-phenylbutyric acid (PBA) inhibit regeneration from both isolated stems and leaves; at lower concentrations some promotion is found with IAA, none with PBA. Shorter exposure of leaves to these compounds results in reversible inhibition, or even an increase in regeneration. IAA and PBA do not counteract each other in their effects.Leaves isolated from intact and decapitated plants show differences in regenerative behavior when treated with IAA. This indicates that IAA could be involved in the inhibition or regeneration from attached leaves.It is concluded that IAA is not the primary factor in the control of correlative inhibition of restitution in the gametophore.

scholarly journals Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Fitmawati Fitmawati ◽  
Nery Sofiyanti ◽  
Rodesia Mustika Roza ◽  
Isnaini Isnaini ◽  
Yulisa Resti Irawan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Natural Resources ◽  
The Other ◽  
Distilled Water ◽  
Water Solvent ◽  
Activity Test ◽  
Dpph Method ◽  
Riau Archipelago

<p><em>Obat Pahit</em> is a potion that has been long commonly consumed by Lingga Malay society for generations as stamina keeper. The most dominant plants found in the packaging of the Obat Pahit were namely <em>Bauhunia semibifida, Cnestis palala</em> and Penawa Root (3 species). This research aimed to investigate and determine activity of antioxidant contents in <em>Obat Pahit</em> from five Traditional Medicine Practitioners (TMPs) in the district of Lingga. The tested samples were mashed then being soaked into 2 types of solvent: distilled water and methanol, containing HCl 1%. DPPH method was also used in this research. Quantitatively antioxidant activity test of <em>Obat Pahit</em> from the five TMPs by using methanol solvent had extremely highest activity compared to the distilled water solvent. The test, using TLC plate by spraying the extract from three dominant plants with 0.1 mM of DPPH solution, produced a pale-yellow spots at a wavelength of 366 nm. On the other hand, the test using HPLC at wavelengths of 230 nm and 280 nm showed the presence of two dominant secondary metabolites contents: flavonoid and phenolic. IC50 (ppm) of <em>Bauhinia semibifida</em> (6.6247), Penawa Root (5.0124) and <em>Cnestis palala</em> (5.9968) were much lower than IC50 of mangosteen’s rind (41.7675), vitamin C (6.6612) and Stimuno drug (8.333). This antioxidant analysis has not been reported previously. This proof contributed greatly to uncovering potentially native natural resources as an indigenous Indonesian drug which is expected to decrease dependence on imported drugs especially imunomodulator, antihypertensive, antidiabet etc. This research would be beneficial and excellent manifestation for the development of natural antioxidant-based medicines from traditional knowledge of Indonesia’s local ethnicities.</p>

scholarly journals Interactions of Irradiance Level and Iron Chelate Source During Shoot Tip Culture of Carica papaya L.

HortScience ◽  
1997 ◽  
Vol 32 (6) ◽  
pp. 1120-1123 ◽  
Author(s):  
B. Castillo ◽  
M.A.L. Smith ◽  
D.L. Madhavi ◽  
U.L. Yadava
Keyword(s):  
Carica Papaya ◽  
Indoleacetic Acid ◽  
Shoot Proliferation ◽  
Leaf Length ◽  
Shoot Tip ◽  
Naphthaleneacetic Acid ◽  
Iron Chelate ◽  
Leaf Chlorophyll ◽  
Proliferation Medium ◽  
Culture Growth

Interactions between irradiance levels (5–40 μmol·m-2·s-1) and iron chelate sources (FeEDTA and FeEDDHA) were observed for Carica papaya shoot tip cultures during both the establishment and proliferation stages of microculture. Reduced levels of irradiance (5 μmol·m-2·s-1) favored shoot tip establishment regardless of the source or level of iron. However, the highest percentage of successful explant establishment (100%), and significantly greater leaf length (1.16 cm; over double the size attained in any other treatment), resulted when a low concentration of FeEDTA alone was used at low irradiance. During the subsequent shoot proliferation stage, however, higher irradiance levels (30 and 40 μmol·m-2·s-1) were required, and FeEDTA failed to support culture growth when used as the sole iron source. The highest multiplication rates (3.6 shoots per explant) and leaf chlorophyll concentrations (0.22 mg/g fresh mass), and significantly improved shoot quality were achieved at 30 μmol·m-2·s-1 irradiance when both iron chelate formulations were combined (each at a 100 μM concentration) in the proliferation medium. Chemical names used: benzylamino purine (BA); ferric disodium ethylenediamine tetraacetate or FeNa2EDTA (FeEDTA); ferric monosodium ethylenediamine di(o-hydroxyphenylacetate), (FeNaEDDHA) or Sequestrene 138Fe (FeEDDHA); indoleacetic acid (IAA); 1-naphthaleneacetic acid (NAA).

scholarly journals Potassium Cyanide (KCN) Content in Coral Reefs and Its Effect on The Abundance of Indicator-Fishes in The Anambas Islands

2021 ◽  
Vol 5 (1) ◽  
pp. 214
Author(s):  
Rizki Dimas Permana ◽  
Sunarto Sunarto ◽  
Syawalludin Alisyahbana Harahap ◽  
Indah Riyantini ◽  
Yuwanda Ilham
Keyword(s):  
Coral Reef ◽  
Negative Correlation ◽  
Protected Area ◽  
Marine Protected Area ◽  
Potassium Cyanide ◽  
Distilled Water ◽  
Coral Reef Ecosystem ◽  
Visual Census ◽  
Underwater Visual Census ◽  
Reef Ecosystem

The coral reef ecosystem has been continuously degraded in various parts of Indonesia, including the Anambas Islands. This research aimed to discover the content of Potassium Cyanide (KCN) accumulated on corals and the effect on indicator fishes abundance in the Anambas Islands. This research was conducted at 25 observation stations in the Anambas Islands National Marine Protected Area. The potassium cyanide (KCN) content was tested with the principle of titration and distillation. The coral samples used ranged from 10-20 g diluted in 100-200 ml distilled water. The method used to determine the abundance of indicator fish was underwater visual census or UVC, which recorded fish in every station. This research recorded 307 fish individuals from 14 species of the Chaetodontidae family. Potassium Cyanide's content on corals was high ranging from 0,009-0,032 mg/L with an average 0,0205 mg/L. We concluded that there was a negative correlation between the content of Potassium Cyanide  (KCN) on corals and indicator fishes abundance, which means the higher the Potassium Cyanide content (KCN) is on corals, the lower the indicator fishes abundance will point out.

Osteoprotective effects of kefir fortified with omega-3 and vitamin C in ovariectomized rats

2021 ◽  
pp. 1-10
Author(s):  
Mahsa Moazen ◽  
Zohreh Mazloom ◽  
Nader Tanideh ◽  
Mohammad Hossein Dabbaghmanesh ◽  
Samane Rahmdel ◽  
...  
Keyword(s):  
Vitamin C ◽  
Bone Mineral ◽  
Ovariectomized Rats ◽  
Distilled Water ◽  
Omega 3 ◽  
Mineral Density ◽  
Amino Terminal ◽  
Tnf Α ◽  
Vit C

Abstract. Nutritional interventions can be valuable for the prevention of postmenopausal osteoporosis. This study aimed to investigate the effects of kefir fortified with omega-3 and vitamin C on the bone and uterus parameters of ovariectomized rats. Seventy-seven female Sprague–Dawley rats were ovariectomized or sham-operated. The ovariectomized rats were assigned to six groups and received 1 ml/day of distilled water (OVX group), milk, kefir, kefir fortified with omega-3 (kefir+ω3), kefir fortified with vitamin C (kefir+vit-C) or kefir fortified with omega-3 and vitamin C (kefir+ω3+vit-C) for 12 weeks. The sham group also received 1ml/day of distilled water. Subsequently, bone mineral content (BMC) and bone mineral density (BMD) of various bones were assessed. Femurs and uteri were harvested for bone ash analysis and histopathological examinations, respectively. Sera were analyzed for carboxy-terminal cross-linked telopeptide of type 1 collagen, procollagen type 1 amino-terminal propeptide, calcium, phosphorous, tumor necrosis factor-α (TNF-α) and total antioxidant capacity levels. Ovariectomy resulted in significant reduction in bone density (P<0.05). Kefir+ω3+vit-C significantly improved BMC of lumbar spine (0.699±0.027 g compared with 0.580±0.018 in the OVX group), and kefir, kefir+vit-C and kefir+ω3+vit-C significantly increased BMD of tibia (0.118±0.003 g/cm2, 0.119±0.001 and 0.120±0.004 compared with 0.102±0.005 in the OVX group). Moreover, ovariectomy markedly elevated TNF-α level, which was significantly reversed by kefir+ω3+vit-C. Significant atrophy of the uterus was observed following ovariectomy, although the uterus parameters did not change by any of the interventions. In conclusion, kefir fortified with omega-3 and vitamin C may have protective effects against bone loss through suppressing inflammation.

Sign in / Sign up

Export Citation Format

Share Document