FURTHER STUDIES ON THE NITROGEN SOURCE FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY SUBMERGED CULTURE OF ASPERGILLUS NIGER

1952 ◽  
Vol 30 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Ping Shu
Keyword(s):  
Amino Acids ◽  
Aspergillus Niger ◽  
Nitrogen Source ◽  
Submerged Culture ◽  
Ammonium Acetate ◽  
Experimental Results ◽  
Alpha Amylase ◽  
Amylolytic Enzymes ◽  
Limit Dextrinase ◽  
High Yields

The experimental results obtained in this study indicate that proteins or amino acids are not necessary for the production of high yields of the amylolytic enzymes, alpha-amylase, maltase, and limit dextrinase. However, in cultures which produce appreciable transient acids during the fermentation the use of a nitrogen source which is a potential alkali donor greatly increases the yield of alpha-amylase. Ammonium acetate fulfills this requirement, and under appropriate conditions it gives high yields of alpha-amylase, maltase, and limit dextrinase. The accumulation of maltase and limit dextrinase is inhibited when the pH of the medium rises above eight, whereas an acid pH as low as four is still suitable for their production.

STUDIES ON CARBON AND NITROGEN SOUCES FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY SUBMERGED CULTURE OF ASPERGILLUS NIGER

1951 ◽  
Vol 29 (2) ◽  
pp. 113-124 ◽  
Author(s):  
P. Shu ◽  
A. C. Blackwood
Keyword(s):  
Aspergillus Niger ◽  
Nitrogen Source ◽  
Inorganic Nitrogen ◽  
Maximum Yield ◽  
Carbohydrate Source ◽  
Amylolytic Enzymes ◽  
Fermentation Time ◽  
Hydrolyzed Protein ◽  
Quantitative Analyses ◽  
Limit Dextrinase

Aspergillus niger PRL 558 was used in the production of starch saccharifying enzymes. The culture was grown on 100 ml. of medium in 500 ml. Erlenmeyer flasks agitated and aerated on a rotary shaker at 35° C. Quantitative analyses for amylase, maltase, and limit dextrinase were determined on the culture filtrates. Variations in the specific type of carbohydrate source affected the yield of amylase markedly, and of maltase to a lesser extent. The production of limit dextrinase is the least dependent on the carbohydrate source. Maltose or compounds constituted of maltose units are essential for producing a maximum yield of amylase. The yields of the enzymes are related to the degree of the availability of the nitrogen source. Highest enzyme yields are obtained with hydrolyzed protein. However, the production of amylase and maltase is further stimulated by adding inorganic nitrogen compounds such as ammonium nitrate and sodium nitrate as a supplementary nitrogen source. The amounts of maltase and amylase obtained are controlled also by varying the fermentation time as well as the carbohudrate and protein content of the medium.

Access to 2-Arylquinazolines via Catabolism/Reconstruction of Amino Acids with Insertion of Dimethyl Sulfoxide

2021 ◽  
Author(s):  
Jin-Tian Ma ◽  
Li-Sheng Wang ◽  
Zhi Chai ◽  
Xin-Feng Chen ◽  
Bo-Cheng Tang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dimethyl Sulfoxide ◽  
Nitrogen Source ◽  
Carbon And Nitrogen ◽  
First Time

Quinazoline skeletons are synthesized by amino acids catabolism/reconstruction combined with dimethyl sulfoxide insertion/cyclization for the first time. The amino acid acts as a carbon and nitrogen source through HI-mediated catabolism...

scholarly journals Adsorption of amino acids on MFI-type zeolite: DFT calculations and experimental results

2012 ◽  
Vol 4 (S1) ◽  
Author(s):  
Kai Stueckenschneider ◽  
Achim Zielesny ◽  
Gerhard Schembecker
Keyword(s):  
Amino Acids ◽  
Dft Calculations ◽  
Experimental Results ◽  
Type Zeolite

Natural Vulcanization Accelerators in Hevea Latex

1948 ◽  
Vol 21 (4) ◽  
pp. 853-859
Author(s):  
R. F. A. Altman
Keyword(s):  
Amino Acids ◽  
Experimental Results ◽  
The Other ◽  
Active Nitrogen ◽  
Nitrogen Bases ◽  
Other Hand ◽  
Decomposition Processes ◽  
Volatile Products ◽  
Nitrogenous Substances ◽  
The One

Abstract As numerous investigators have shown, some of the nonrubber components of Hevea latex have a decided accelerating action on the process of vulcanization. A survey of the literature on this subject points to the validity of certain general facts. 1. Among the nonrubber components of latex which have been investigated, certain nitrogenous bases appear to be most important for accelerating the rate of vulcanization. 2. These nitrogen bases apparently occur partly naturally in fresh latex, and partly as the result of putrefaction, heating, and other decomposition processes. 3. The nitrogen bases naturally present in fresh latex at later stages have been identified by Altman to be trigonelline, stachhydrine, betonicine, choline, methylamine, trimethylamine, and ammonia. These bases are markedly active in vulcanization, as will be seen in the section on experimental results. 4. The nitrogenous substances formed by the decomposition processes have only partly been identified, on the one hand as tetra- and pentamethylene diamine and some amino acids, on the other hand as alkaloids, proline, diamino acids, etc. 5. It has been generally accepted that these nitrogenous substances are derived from the proteins of the latex. 6. Decomposition appears to be connected with the formation of a considerable amount of acids. 7. The production of volatile nitrogen bases as a rule accompanies the decomposition processes. These volatile products have not been identified. 8. The active nitrogen bases, either already formed or derived from complex nitrogenous substances, seem to be soluble in water but only slightly soluble in acetone.

Diauxic production of glucose oxidase by Aspergillus niger in submerged culture

2002 ◽  
Vol 31 (5) ◽  
pp. 615-620 ◽  
Author(s):  
J. Mirón ◽  
M.P. González ◽  
L. Pastrana ◽  
M.A. Murado
Keyword(s):  
Aspergillus Niger ◽  
Glucose Oxidase ◽  
Submerged Culture

Canonical interrelationships in morphological characters, yield and nutritional components of corn

2021 ◽  
Vol 8 ◽  
pp. 1-17
Author(s):  
Ivan Carvalho ◽  
José Antonio Gonzalez da Silva ◽  
Murilo Vieira Loro ◽  
Marlon Vinícius Rosa Sarturi ◽  
Danieli Jacoboski Hutra ◽  
...  
Keyword(s):  
Amino Acids ◽  
Bioactive Compounds ◽  
Yield Components ◽  
Zinc Content ◽  
Essential Amino Acids ◽  
Morphological Characters ◽  
Soluble Solids ◽  
World Population ◽  
Canonical Correlations ◽  
High Yields

The increase in the world population, the need to increase food production, both in quantity and quality, becomes increasingly prominent. The objective of this work was to identify the canonical correlations between yield components, morphological characters, micronutrients, bioactive compounds and amino acids in corn. The experimental design used was a randomized block containing 11 treatments arranged in three replications. The treatments consisted of 11 Top Crosses hybrid genotypes, these being made through crosses directed between a narrow genetic base tester hybrid for specific combining ability with 11 S5 inbred lines. It is inferred that groups considered yield components, secondary traits, bioactive compounds, micronutrients and amino acids are dependent. Promising characters are identified for the corn breeding for high yields, nutritional and energetic quality of corn grains. The indirect selection of grains with additions in essential amino acids can be directed to plants with superiority in height, mass and width of grains, phenols, flavonoids, soluble solids and zinc content.

scholarly journals Synthesis of Imidazol-2-yl Amino Acids by Using Cells from Alkane-Oxidizing Bacteria

2003 ◽  
Vol 69 (3) ◽  
pp. 1670-1679 ◽  
Author(s):  
Annett Mikolasch ◽  
Elke Hammer ◽  
Frieder Schauer
Keyword(s):  
Amino Acids ◽  
Chemical Structure ◽  
Rhodococcus Erythropolis ◽  
Nocardia Asteroides ◽  
Side Chain ◽  
Butanoic Acid ◽  
Transformation Pathway ◽  
Nuclear Magnetic Resonance Spectrometry ◽  
Magnetic Resonance Spectrometry ◽  
High Yields

ABSTRACT Sixty-one strains of alkane-oxidizing bacteria were tested for their ability to oxidize N-(2-hexylamino-4-phenylimidazol-1-yl)-acetamide to imidazol-2-yl amino acids applicable for pharmaceutical purposes. After growth with n-alkane, 15 strains formed different imidazol-2-yl amino acids identified by chemical structure analysis (mass and nuclear magnetic resonance spectrometry). High yields of imidazol-2-yl amino acids were produced by the strains Gordonia rubropertincta SBUG 105, Gordonia terrae SBUG 253, Nocardia asteroides SBUG 175, Rhodococcus erythropolis SBUG 251, and Rhodococcus erythropolis SBUG 254. Biotransformation occurred via oxidation of the alkyl side chain and produced 1-acetylamino-4-phenylimidazol-2-yl-6-aminohexanoic acid and the butanoic acid derivative. In addition, the acetylamino group of these products and of the substrate was transformed to an amino group. The product pattern as well as the transformation pathway of N-(2-hexylamino-4-phenylimidazol-1-yl)-acetamide differed in the various strains used.

scholarly journals Cyclopeptide Derivatives from the Sponge-Derived Fungus Acremonium persicinum F10

Marine Drugs ◽  
2021 ◽  
Vol 19 (10) ◽  
pp. 537
Author(s):  
Yingxin Li ◽  
Zhiyong Li
Keyword(s):  
Amino Acids ◽  
Aspergillus Niger ◽  
Diffraction Analysis ◽  
2D Nmr ◽  
Ferric Ions ◽  
Single Crystal Diffraction ◽  
X Ray ◽  
Planar Structures ◽  
Anti Fungal

Cyclopeptides usually play a pivotal role, either in the viability or virulence of fungi. Two types of cyclopeptides, six new hydroxamate siderophore cyclohexapeptides (1–6), including acremonpeptides E and F, and their complexes with aluminum and ferric ions; one new cyclic pentapeptolide, aselacin D (9); together with a known compound, aselacin C (10), were isolated and characterized from the sponge-derived fungus Acremonium persicinum F10. In addition, two new siderophore analogues chelating gallium ions (Ga3+), Ga (Ⅲ)-acremonpeptide E (7) and Ga (Ⅲ)-acremonpeptide F (8), using isolated acremonpeptides E and F, were prepared. The planar structures of 1–10 were elucidated by HRESIMS and (1D and 2D) NMR. The absolute configurations of amino acids were determined by means of the advanced Marfey’s method and X-ray single-crystal diffraction analysis. X-ray fluorescence (XRF) spectrometer was performed to disclose the elements of compound 1, indicating the existence of aluminum (Al). Al (Ⅲ)-acremonpeptides E (1), Ga (Ⅲ)-acremonpeptides E (5), Al (Ⅲ)-acremonpeptide F (7), and Ga (Ⅲ)-acremonpeptide F (8) displayed high in vitro anti-fungal activities, which are comparable to amphotericin B, against Aspergillus fumigatus and Aspergillus niger.

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