STUDIES ON CARBON AND NITROGEN SOUCES FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY SUBMERGED CULTURE OF ASPERGILLUS NIGER

1951 ◽  
Vol 29 (2) ◽  
pp. 113-124 ◽  
Author(s):  
P. Shu ◽  
A. C. Blackwood
Keyword(s):  
Aspergillus Niger ◽  
Nitrogen Source ◽  
Inorganic Nitrogen ◽  
Maximum Yield ◽  
Carbohydrate Source ◽  
Amylolytic Enzymes ◽  
Fermentation Time ◽  
Hydrolyzed Protein ◽  
Quantitative Analyses ◽  
Limit Dextrinase

Aspergillus niger PRL 558 was used in the production of starch saccharifying enzymes. The culture was grown on 100 ml. of medium in 500 ml. Erlenmeyer flasks agitated and aerated on a rotary shaker at 35° C. Quantitative analyses for amylase, maltase, and limit dextrinase were determined on the culture filtrates. Variations in the specific type of carbohydrate source affected the yield of amylase markedly, and of maltase to a lesser extent. The production of limit dextrinase is the least dependent on the carbohydrate source. Maltose or compounds constituted of maltose units are essential for producing a maximum yield of amylase. The yields of the enzymes are related to the degree of the availability of the nitrogen source. Highest enzyme yields are obtained with hydrolyzed protein. However, the production of amylase and maltase is further stimulated by adding inorganic nitrogen compounds such as ammonium nitrate and sodium nitrate as a supplementary nitrogen source. The amounts of maltase and amylase obtained are controlled also by varying the fermentation time as well as the carbohudrate and protein content of the medium.

FURTHER STUDIES ON THE NITROGEN SOURCE FOR THE PRODUCTION OF AMYLOLYTIC ENZYMES BY SUBMERGED CULTURE OF ASPERGILLUS NIGER

1952 ◽  
Vol 30 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Ping Shu
Keyword(s):  
Amino Acids ◽  
Aspergillus Niger ◽  
Nitrogen Source ◽  
Submerged Culture ◽  
Ammonium Acetate ◽  
Experimental Results ◽  
Alpha Amylase ◽  
Amylolytic Enzymes ◽  
Limit Dextrinase ◽  
High Yields

The experimental results obtained in this study indicate that proteins or amino acids are not necessary for the production of high yields of the amylolytic enzymes, alpha-amylase, maltase, and limit dextrinase. However, in cultures which produce appreciable transient acids during the fermentation the use of a nitrogen source which is a potential alkali donor greatly increases the yield of alpha-amylase. Ammonium acetate fulfills this requirement, and under appropriate conditions it gives high yields of alpha-amylase, maltase, and limit dextrinase. The accumulation of maltase and limit dextrinase is inhibited when the pH of the medium rises above eight, whereas an acid pH as low as four is still suitable for their production.

Production of L-Asparaginase from Aspergillus Niger using Agro Wastes By-Products in Submerged Fermentation Process

2013 ◽  
Vol 62 (2) ◽  
Author(s):  
Muhammad Anjum Zia Anjum Zia ◽  
Rabia Bashir ◽  
Ishtiaq Ahmed ◽  
Tehreema Iftikhar
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Fermentation Process ◽  
Corn Steep Liquor ◽  
Maximum Yield ◽  
Growth Media ◽  
Organic Salts ◽  
Waste Products ◽  
Fermentation Time ◽  
By Products

The project was carried out to obtain maximum yield of L-asparaginase from Aspergillus niger using by-products of agro wastes incorporated with organic salts in submerged fermentation process. The main objective of the project was to study the kinetic parameters of L-asparaginase productivity. After optimization maximum enzyme activity (2.83U/mL±0.065) was achieved using corn steep liquor as a substrate and with 4% inoculum, pH 6.5, 1% substrate concentration, 96 hrs fermentation time period and 1% glucose was used as additional supplement to the growth media to obtain better yield of L-asparaginase. This study showed that glucose concentration beyond 1% suppressed the enzymatic activity. From the results it can be concluded that L-Asparaginase production was optimized when cheap agro-waste products were used as a substrate at low concentrations and under acidic conditions. Its relative stability in acidic pH conditions make it ideal for applications in health care systems and pharmaceutical industry.

scholarly journals Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

2017 ◽  
Vol 7 (5) ◽  
pp. 17
Author(s):  
Mirza M.V. Baig ◽  
Aniruddha Ratnakar Apastambh
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Solid Substrate ◽  
Inorganic Nitrogen Source ◽  
Pectic Enzymes

The production of Pectic enzymes by Aspergillus niger was studied under solid state fermentation (SSF). The effect of fermentation condition such as substrate concentration, inoculum volume, incubation time, moistening agent, inducers and organic and inorganic nitrogen sources was studied for enzyme production. Culture conditions were optimized for maximal yield of enzyme. The solid substrate wheat bran was most suitable for pectic enzyme production under SSF. Enzyme production was found maximum after 10 days of incubation. Lactose was found to be most effective as inducer. Gelatin as organic nitrogen source and ammonium nitrate as inorganic nitrogen source yielded high enzyme titres.

THE PROTEOLYTIC ENZYMES OF MICROORGANISMS: II. FACTORS AFFECTING THE PRODUCTION OF PROTEASES IN SUBMERGED CULTURE

1950 ◽  
Vol 28c (6) ◽  
pp. 586-599 ◽  
Author(s):  
W. M. Dion
Keyword(s):  
Culture Medium ◽  
Proteolytic Enzymes ◽  
Inorganic Nitrogen ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Protease Production ◽  
Carbohydrate Source ◽  
Factors Affecting ◽  
Main Factors ◽  
High Yields

The main factors that influence the production of proteolytic enzymes by a few selected cultures have been studied. The time taken to reach the maximum yield of proteases is dependent upon the growth rate of each organism, and varies from two to five days. The fungi tested require the presence of an easily available carbohydrate source in addition to a protein substrate in order to produce high yields of proteolytic enzymes. The Streptomyces cultures will produce proteases in the absence of a carbohydrate source, but yields are generally low. The fungi studied will not produce significant amounts of proteases when grown on predominately inorganic nitrogen sources in contrast with the Streptomyces cultures, one of which produced almost as high yields of proteolytic enzymes when grown with sodium nitrate as when grown with Klim. Of a number of protein sources Klim and malt sprouts provided the best media for protease production. The temperature of incubation and pH of the culture medium are also important factors affecting the yield of proteolytic enzymes.

scholarly journals Lovastatin Production byAspergillus terreusUsing Agro-Biomass as Substrate in Solid State Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Mohammad Faseleh Jahromi ◽  
Juan Boo Liang ◽  
Yin Wan Ho ◽  
Rosfarizan Mohamad ◽  
Yong Meng Goh ◽  
...  
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Initial Moisture Content ◽  
Fermentation Time ◽  
Maximum Production ◽  
Additional Nitrogen

Ability of two strains ofAspergillus terreus(ATCC 74135 and ATCC 20542) for production of lovastatin in solid state fermentation (SSF) using rice straw (RS) and oil palm frond (OPF) was investigated. Results showed that RS is a better substrate for production of lovastatin in SSF. Maximum production of lovastatin has been obtained usingA. terreusATCC 74135 and RS as substrate without additional nitrogen source (157.07 mg/kg dry matter (DM)). Although additional nitrogen source has no benefit effect on enhancing the lovastatin production using RS substrate, it improved the lovastatin production using OPF with maximum production of 70.17 and 63.76 mg/kg DM forA. terreusATCC 20542 andA. terreusATCC 74135, respectively (soybean meal as nitrogen source). Incubation temperature, moisture content, and particle size had shown significant effect on lovastatin production (P<0.01) and inoculums size and pH had no significant effect on lovastatin production (P>0.05). Results also have shown that pH 6, 25°C incubation temperature, 1.4 to 2 mm particle size, 50% initial moisture content, and 8 days fermentation time are the best conditions for lovastatin production in SSF. Maximum production of lovastatin using optimized condition was 175.85 and 260.85 mg/kg DM forA. terreusATCC 20542 and ATCC 74135, respectively, using RS as substrate.

scholarly journals PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY ASPERGILLUS NIGER AND RHIZOPUS SP. BY SOLID STATE FERMENTATION OF PRICKLY PEAR

2016 ◽  
Vol 29 (1) ◽  
pp. 222-233 ◽  
Author(s):  
TAMIRES CARVALHO DOS SANTOS ◽  
GEORGE ABREU FILHO ◽  
AILA RIANY DE BRITO ◽  
AURELIANO JOSÉ VIEIRA PIRES ◽  
RENATA CRISTINA FERREIRA BONOMO ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Cellulase Production ◽  
Cellulolytic Enzymes ◽  
Optimum Conditions ◽  
Thermostable Enzymes ◽  
Fermentation Time ◽  
Rhizopus Sp

ABSTRACT: Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.

Sign in / Sign up

Export Citation Format

Share Document