Glomus indicum, a new arbuscular mycorrhizal fungus

Botany ◽  
2010 ◽  
Vol 88 (2) ◽  
pp. 132-143 ◽  
Author(s):  
Janusz Błaszkowski ◽  
Tesfaye Wubet ◽  
Variampally Sankar Harikumar ◽  
Przemysław Ryszka ◽  
François Buscot
Keyword(s):  
Plantago Lanceolata ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Single Species ◽  
Spore Wall ◽  
Fungal Species ◽  
Phylogenetic Position ◽  
Single Spore ◽  
North East ◽  
Rdna Sequences

A new arbuscular mycorrhizal fungal species of the genus Glomus , Glomus indicum (Glomeromycota), forming small, hyaline spores in hypogeous aggregates is described and illustrated. The spores are globose to subglobose, (17–)32(–52) µm in diameter, rarely egg-shaped, oblong to irregular, 17–38 µm × 19–43 µm. The single spore wall of G. indicum consists of two hyaline layers: a mucilaginous, short-lived, thin outer layer staining pinkish to pink in Melzer's reagent and a laminate, smooth, permanent, thicker inner layer. Glomus indicum was found in the rhizosphere of Euphorbia heterophylla  L. naturally growing in coastal sands of Alappuzha in Kerala State of South India and Lactuca sativa  L. cultivated in Asmara, Eritrea, North East Africa. In single-species cultures with Plantago lanceolata  L. as the host plant, G. indicum formed vesicular-arbuscular mycorrhiza. Molecular analysis of the phylogenetic position of G. indicum based on both SSU and ITS rDNA sequences showed the fungus to be a new species with its own cluster. Besides the sites where the spores were observed, sequence types belonging to the G. indicum cluster were documented from environmental samples mainly in the USA, Estonia, and Australia, suggesting the wide occurrence of the species. A key to all known species of the Glomeromycota producing hyaline to light-coloured glomoid spores is provided.

scholarly journals Glomus arenarium, a new species in Glomales (Zygomycetes)

2014 ◽  
Vol 70 (2) ◽  
pp. 97-101 ◽  
Author(s):  
Janusz Błaszkowski ◽  
Mariusz Tadych ◽  
Tadeusz Madej
Keyword(s):  
Arbuscular Mycorrhizae ◽  
Plantago Lanceolata ◽  
Sand Dunes ◽  
Arbuscular Mycorrhizal ◽  
Middle Layer ◽  
Single Species ◽  
Spore Wall ◽  
Fungal Species ◽  
Innermost Layer ◽  
Arbuscular Mycorrhizal Fungal

A new ectocarpic arbuscular mycorrhizal fungal species, <em>Glomus arenarium</em> (<em>Glomales</em>, <em>Zygomycetes</em>), was recovered from maritime sand dunes of northern Poland. <em>Glomus arenarium</em> forms spores with a narrow and hyaline subtending hypha. Spores are orange to raw umber, globose to subglobose, (55-)97(-120) µm diam or ovoid, 65-105 x 95-140 µm. Their wall consists of three layers: a hyaline outermost layer present only in very young spores, a semiflexible, hyaline middle layer rarely present in mature spores, and a permanent, laminate, orange to raw umber innermost layer. No spore wall layers of <em>G. arenarium</em> reagent. This fungus formed spores and arbuscular mycorrhizae in single-species pot cultures with Plantago lanceolata.

scholarly journals Bacteria Associated with Spores of the Arbuscular Mycorrhizal Fungi Glomus geosporum and Glomus constrictum

2005 ◽  
Vol 71 (11) ◽  
pp. 6673-6679 ◽  
Author(s):  
David Roesti ◽  
Kurt Ineichen ◽  
Olivier Braissant ◽  
Dirk Redecker ◽  
Andres Wiemken ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Plantago Lanceolata ◽  
Arbuscular Mycorrhizal ◽  
Spore Wall ◽  
Wall Layer ◽  
Single Spore ◽  
Bacterial Populations ◽  
Glomus Geosporum

ABSTRACT Spores of the arbuscular mycorrhizal fungi (AMF) Glomus geosporum and Glomus constrictum were harvested from single-spore-derived pot cultures with either Plantago lanceolata or Hieracium pilosella as host plants. PCR-denaturing gradient gel electrophoresis analysis revealed that the bacterial communities associated with the spores depended more on AMF than host plant identity. The composition of the bacterial populations linked to the spores could be predominantly influenced by a specific spore wall composition or AMF exudate rather than by specific root exudates. The majority of the bacterial sequences that were common to both G. geosporum and G. constrictum spores were affiliated with taxonomic groups known to degrade biopolymers (Cellvibrio, Chondromyces, Flexibacter, Lysobacter, and Pseudomonas). Scanning electron microscopy of G. geosporum spores revealed that these bacteria are possibly feeding on the outer hyaline spore layer. The process of maturation and eventual germination of AMF spores might then benefit from the activity of the surface microorganisms degrading the outer hyaline wall layer.

Acaulospora papillosa, a new mycorrhizal fungus from NE Brazil, and Acaulospora rugosa from Norway

Phytotaxa ◽  
2016 ◽  
Vol 260 (1) ◽  
pp. 14 ◽  
Author(s):  
CAMILLA M.R. PEREIRA ◽  
LEONOR C. MAIA ◽  
IVÁN SÁNCHEZ-CASTRO ◽  
JAVIER PALENZUELA ◽  
DANIELLE K.A. SILVA ◽  
...  
Keyword(s):  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Single Species ◽  
Spore Wall ◽  
Wall Layer ◽  
Northeastern Brazil ◽  
Tropical Atlantic ◽  
Spore Surface ◽  
Phylogenetic Placement ◽  
Light Yellow

A new arbuscular mycorrhizal species, Acaulospora papillosa, was isolated from the biological reserve ‘Saltinho’ within a coastal tropical Atlantic forest of the ‘Mata Atlântica’ biome in Pernambuco State of Northeastern Brazil. It was trapped and propagated as single species cultures on Zea mays. The spores are yellow white to light yellow to creamy, globose to subglobose, 69–100(–110) × 65–93(–101) µm. The spore surface is roughened as crowded with fine papillae, which are formed on the outermost, evanescent to semi-persistent spore wall layer. These papillae may disintegrate or completely disappear as the spores age and the layer becomes completely evanescent. Phylogenetically, the fungus clusters together with several small-spored Acaulospora species having smooth spore surfaces, such as A. delicata, A. longula, A. morrowiae and A. mellea. In the Acaulospora clade, A. papillosa is the third taxon known to have a roughened spore surface, in addition to A. dilatata and A. rugosa. The phylogenetic placement of A. rugosa is provided, together with colored illustrations of the spore morphology. The isolation of A. papillosa from such protected nature reserves as ‘Saltinho’ further supports the need to protect these areas and determine the biodiversity of beneficial microorganisms.

scholarly journals Sieverdingia gen. nov., S. tortuosa comb. nov., and Diversispora peloponnesiaca sp. nov. in the Diversisporaceae (Glomeromycota)

2019 ◽  
Vol 18 (11) ◽  
pp. 1363-1382 ◽  
Author(s):  
Janusz Błaszkowski ◽  
Piotr Niezgoda ◽  
Jéssica Nunes de Paiva ◽  
Kássia Jéssica Galdino da Silva ◽  
Raquel Cordeiro Theodoro ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Spore Wall ◽  
Molecular Characteristics ◽  
Rdna Sequences ◽  
The Family ◽  
Innermost Layer ◽  
Definition Of

Abstract Phylogenetic analyses of 18S–ITS–28S nuc rDNA sequences indicated that the arbuscular mycorrhizal fungus originally described as Glomus tortuosum and later transferred to the genus Corymbiglomus represents a separate, previously unrecognized clade at the rank of genus in the family Diversisporaceae (order Diversisporales, phylum Glomeromycota). The analyses located the clade between clades representing the genera Desertispora and Redeckera. Consequently, a new genus, Sieverdingia, was erected, with S. tortuosa comb. nov. The unique morphological feature of S. tortuosa is the formation of glomoid-like spores with a single-layered spore wall covered with a hyphal mantle. Importantly, the erection of Sieverdingia clarified the definition of Corymbiglomus, which currently consists of three species producing glomoid-like spores with one, three- to four-layered spore wall. The features of the innermost layer, which is hyaline, laminate, flexible to semi-flexible, indicate that it is a synapomorphy of Corymbiglomus. The definitions of Corymbiglomus and its species were emended. Moreover, the distribution of S. tortuosa and the three species of Corymbiglomus was discussed based on own studies, literature data, and molecular sequences deposited in public databases. We concluded that the distribution of S. tortuosa and C. globiferum known in environmental studies based on their partial 28S nuc rDNA sequences only may be understated because the main molecular characteristics distinguishing these species reside outside the 28S region. Finally, we described a new species in the genus Diversispora originating from Mediterranean dunes of the Peloponnese peninsula, Greece. The same phylogenetic analyses mentioned above indicated that the closest relative of the new species, producing dark-coloured spores, is D. clara, whose spores are creamy white at most.

Microkamienskia gen. nov. and Microkamienskia peruviana, a new arbuscular mycorrhizal fungus from Western Amazonia

Nova Hedwigia ◽  
2019 ◽  
Vol 109 (3) ◽  
pp. 355-368 ◽  
Author(s):  
Mike Anderson Corazon-Guivin ◽  
Agustin Cerna-Mendoza ◽  
Juan Carlos Guerrero-Abad ◽  
Adela Vallejos-Tapullima ◽  
Santos Carballar-Hernández ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Single Species ◽  
Spore Wall ◽  
Wall Layer ◽  
Am Fungus ◽  
Small Spore ◽  
Plukenetia Volubilis

A new arbuscular mycorrhizal (AM) fungus, Microkamienskia peruviana, was detected in bait cultures for arbuscular mycorrhizal fungi established with rhizospheric soil substrates of the inka nut (Plukenetia volubilis). The field soil derived from three agricultural plantations in the Amazonia lowlands of the province Lamas, San Martin State, in Peru. The fungus was subsequently propagated in single species cultures on Sorghum sp., Brachiaria sp.,Medicago sativa and P. volubilis as host plants. The new species differentiates hyaline spores regularly in spore clusters, up to 500–800×400–600 μm. The spores are 16–31(–36)×13–29(–35) μm in diam, formed on cylindrical or slightly funnel-shaped hyphae, without a septum at or close to the spore base. Phylogenetically, the new fungus belongs to a new genus, named Microkamienskia, which has as type species M. perpusilla comb. nov. and to which also M. divaricata comb. nov. belongs. Both are transferred from Kamienskia to Microkamienskia in the present study. The new fungus can be identified by the ballooning semi-persistent to evanescent outer spore wall layer in PVLG-based mountants that is not known for the other species of these two genera, nor for any other glomeromycotan species of similar small spore sizes. Kamienskia and Microkamienskia species can be distinguished by their position in the phylogenetic tree and by hyaline spores, open pores at the spore bases and in the subtending hyphae, and by their spore sizes that are for Microkamienskia among the smallest spore sizes so far detected for AM fungi (15–35 μm).

scholarly journals Parasitism of spores of the vesicular-arbuscular mycorrhizal fungus, Glomus dimorphicum

2005 ◽  
Vol 72 (1) ◽  
pp. 27-32 ◽  
Author(s):  
S.M. Boyetchko ◽  
J.P. Tewari
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Host Response ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Spore Wall ◽  
Vesicular Arbuscular ◽  
Scanning Electron

Spores of Glomus dimorphicum were examined for parasitism. Light and scanning electron microscopy revealed perforations, approximately 0.25 to 1.0 µm in diameter, in the spore wall. The presence of papillae, a dynamic host response, suggested that the parasitism occurred while the vesicular-arbuscular mycorrhizal fungus was still alive. No filamentous structures were detected in the spores; however, cysts of amoeba-like organisms were found in the spores and were also observed on agar plates on which surface-sterilized spores of G. dimorphicum containing such organisms were placed. It is postulated that an amoeba-like organism was the parasite, since the perforations on the spore wall were minute and no bacteria or fungi were seen inside the spores.

scholarly journals Fungal Lipid Accumulation and Development of Mycelial Structures by Two Arbuscular Mycorrhizal Fungi

2003 ◽  
Vol 69 (11) ◽  
pp. 6762-6767 ◽  
Author(s):  
Ingrid M. van Aarle ◽  
Pål Axel Olsson
Keyword(s):  
Fatty Acid ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Neutral Lipids ◽  
Phospholipid Fatty Acid ◽  
Plantago Lanceolata ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Fungal Species ◽  
Opposite Pattern

ABSTRACT We monitored the development of intraradical and extraradical mycelia of the arbuscular mycorrhizal (AM) fungi Scutellospora calospora and Glomus intraradices when colonizing Plantago lanceolata. The occurrence of arbuscules (branched hyphal structures) and vesicles (lipid storage organs) was compared with the amounts of signature fatty acids. The fatty acid 16:1ω5 was used as a signature for both AM fungal phospholipids (membrane constituents) and neutral lipids (energy storage) in roots (intraradical mycelium) and in soil (extraradical mycelium). The formation of arbuscules and the accumulation of AM fungal phospholipids in intraradical mycelium followed each other closely in both fungal species. In contrast, the neutral lipids of G. intraradices increased continuously in the intraradical mycelium, while vesicle occurrence decreased after initial rapid root colonization by the fungus. S. calospora does not form vesicles and accumulated more neutral lipids in extraradical than in intraradical mycelium, while the opposite pattern was found for G. intraradices. G. intraradices allocated more of its lipids to storage than did S. calospora. Thus, within a species, the fatty acid 16:1ω5 is a good indicator for AM fungal development. The phospholipid fatty acid 16:1ω5 is especially suitable for indicating the frequency of arbuscules in the symbiosis. We propose that the ratio of neutral lipids to phospholipids is more important than is the presence of vesicles in determining the storage status of AM fungi.

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