scholarly journals Parasitism of spores of the vesicular-arbuscular mycorrhizal fungus, Glomus dimorphicum

2005 ◽  
Vol 72 (1) ◽  
pp. 27-32 ◽  
Author(s):  
S.M. Boyetchko ◽  
J.P. Tewari
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Host Response ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Spore Wall ◽  
Vesicular Arbuscular ◽  
Scanning Electron

Spores of Glomus dimorphicum were examined for parasitism. Light and scanning electron microscopy revealed perforations, approximately 0.25 to 1.0 µm in diameter, in the spore wall. The presence of papillae, a dynamic host response, suggested that the parasitism occurred while the vesicular-arbuscular mycorrhizal fungus was still alive. No filamentous structures were detected in the spores; however, cysts of amoeba-like organisms were found in the spores and were also observed on agar plates on which surface-sterilized spores of G. dimorphicum containing such organisms were placed. It is postulated that an amoeba-like organism was the parasite, since the perforations on the spore wall were minute and no bacteria or fungi were seen inside the spores.

Occurrence of Vesicular-Arbuscular Mycorrhizal Fungi in Alberta, Canada

1993 ◽  
Vol 48 (11-12) ◽  
pp. 923-929 ◽  
Author(s):  
S. M. Boyetchko ◽  
J. P. Tewari
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Fungal Species ◽  
Glomus Aggregatum ◽  
X Ray ◽  
Vesicular Arbuscular ◽  
Spore Walls ◽  
Scanning Electron

Abstract Three V A mycorrhizal fungal species were isolated from soils in Alberta, Canada and examined by scanning electron microscopy and energy-dispersive X-ray microanalysis. Mature spores of Glomus aggregatum developed an outer hyaline wall which contained lower levels of calcium than the middle wall. Examination of G. pansihalos spores revealed a lower level of calcium in the outer evanescent wall as compared to the ornamented wall. When spores of Entrophospora infrequens were examined, the wall of the vesicle was found to contain similar levels of calcium as the ornamented wall of the spore. The significance of the results concerning the presence of calcium in mycorrhizal spore walls is discussed, as is the occurrence of the mycorrhizal species.

scholarly journals Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

2003 ◽  
Vol 69 (10) ◽  
pp. 6250-6256 ◽  
Author(s):  
Avram Levy ◽  
Barbara J. Chang ◽  
Lynette K. Abbott ◽  
John Kuo ◽  
Gerry Harnett ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fluorescence Microscopy ◽  
Dna Sequences ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Transmission Electron ◽  
Electron And Fluorescence Microscopy ◽  
Scanning Electron

ABSTRACT Burkholderia species are bacterial soil inhabitants that are capable of interacting with a variety of eukaryotes, in some cases occupying intracellular habitats. Pathogenic and nonpathogenic Burkholderia spp., including B. vietnamiensis, B. cepacia, and B. pseudomallei, were grown on germinating spores of the arbuscular mycorrhizal fungus Gigaspora decipiens. Spore lysis assays revealed that all Burkholderia spp. tested were able to colonize the interior of G. decipiens spores. Amplification of specific DNA sequences and transmission electron microscopy confirmed the intracellular presence of B. vietnamiensis. Twelve percent of all spores were invaded by B. vietnamiensis, with an average of 1.5 × 106 CFU recovered from individual infected spores. Of those spores inoculated with B. pseudomallei, 7% were invaded, with an average of 5.5 × 105 CFU recovered from individual infected spores. Scanning electron and fluorescence microscopy provided insights into the morphology of surfaces of spores and hyphae of G. decipiens and the attachment of bacteria. Burkholderia spp. colonized both hyphae and spores, attaching to surfaces in either an end-on or side-on fashion. Adherence of Burkholderia spp. to eukaryotic surfaces also involved the formation of numerous fibrillar structures.

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