Oomycete and chytrid infections of the marine diatom Pseudo-nitzschia pungens (Bacillariophyceae) from Prince Edward Island, Canada

Botany ◽  
2009 ◽  
Vol 87 (11) ◽  
pp. 1096-1105 ◽  
Author(s):  
Louis A. Hanic ◽  
Satoshi Sekimoto ◽  
Stephen S. Bates
Keyword(s):  
Discharge Tube ◽  
Prince Edward Island ◽  
Dense Body ◽  
Morphological Characteristics ◽  
Marine Diatom ◽  
Cell Infection ◽  
Host Cytoplasm ◽  
Single Discharge ◽  
Thickened Base ◽  
Nitzschia Pungens

Two eukaryotic parasites were found infecting the bloom-forming marine diatom Pseudo-nitzschia pungens (Grunow ex Cleve) Hasle in Prince Edward Island, Canada. The most common was an oomycete; the other, seen once, was a chytrid. The structure of the discharged sporangium of both is remarkably similar. The oomycete parasite caused the host cell to lay down several extra girdle bands as the parasite thallus grew and swelled to form a holocarpic, endobiotic, nonwalled multinucleate thallus within the host cytoplasm. At maturity the thallus formed a single discharge tube with a thickened base and a thin papillate apex. Many biflagaellate zoospores were formed that burst out from the discharge tube. Ultrastructural characteristics of the oomycete thallus include mitochondria with tubular cristae and vesicles with dense body inclusions, features common to the oomycetes. The morphological characteristics and biflagellate condition indicate a placement of this form in the genus Ectrogella . However, neither flagellar mastigonemes nor flagella flimmer vesicles were found. The absence of flimmers may indicate a closer phylogenetic relationship to Haptoglossa , an endoparasitic oomycete of nematodes, the zoospores of which lack flagellar mastigonemes. Cell infection frequencies ranged from 0.6%–15.9% during 1992–1995, at the four sampling sites.

Domoic Acid—A Neurotoxic Amino Acid Produced by the Marine Diatom Nitzschia pungens in Culture

1988 ◽  
Vol 45 (12) ◽  
pp. 2076-2079 ◽  
Author(s):  
D. V. Subba Rao ◽  
M. A. Quilliam ◽  
R. Pocklington
Keyword(s):  
Amino Acid ◽  
Chemical Analysis ◽  
River Water ◽  
Prince Edward Island ◽  
Domoic Acid ◽  
Culture Medium ◽  
Marine Diatom ◽  
Eastern Canada ◽  
Blue Mussels ◽  
Nitzschia Pungens

During late 1987, an outbreak of poisoning resulting from the ingestion of cultivated blue mussels (Mytilus edulis) from a localized area in eastern Canada (Cardigan Bay, Prince Edward Island) was associated with massive blooms of Nitzschia pungens, a widely distributed diatom not previously known to produce toxins; human fatalities resulted. Here we provide proof that the causative agent, domoic acid, is indeed produced by this diatom. Although no domoic acid could be detected (<2 ng∙mL−1) in culture medium (FE) prepared from Cardigan River water, it was found in cultures of Nitzschia pungens grown in this medium at concentrations ranging from 0.03 to 0.8 pg∙cell−1 in various separate cultures harvested for chemical analysis 7–68 d after inoculation.

scholarly journals Sexual reproduction and genetic polymorphism within the cosmopolitan marine diatom Pseudo-nitzschia pungens

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jin Ho Kim ◽  
Penelope Ajani ◽  
Shauna A. Murray ◽  
Joo-Hwan Kim ◽  
Hong Chang Lim ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Sexual Reproduction ◽  
Marine Diatom ◽  
Nitzschia Pungens

Sabbea gen. nov., a new diatom genus (Bacillariophyta) from continental Antarctica

Phytotaxa ◽  
2019 ◽  
Vol 418 (1) ◽  
pp. 42-56 ◽  
Author(s):  
JORDAN BISHOP ◽  
KATEŘINA KOPALOVÁ ◽  
JOSHUA P. DARLING ◽  
NICHOLAS O. SCHULTE ◽  
TYLER J. KOHLER ◽  
...  
Keyword(s):  
New Genus ◽  
Morphological Characteristics ◽  
Sensu Stricto ◽  
Morphological Features ◽  
Marine Diatom ◽  
Continental Antarctica ◽  
Diatom Genus ◽  
Antarctic Continent ◽  
Vestfold Hills ◽  
The Antarctic

The non-marine diatom flora of the Antarctic Continent includes several endemic taxa recorded over the past 100 years. One of these taxa, Navicula adminensis D.Roberts & McMinn, was described from the Vestfold Hills, East Antarctica. Detailed light and scanning electron microscopy observations have shown that based on its morphological features, the species does not belong to the genus Navicula sensu stricto. To determine the most closely related genera to N. adminensis, the morphological features of Adlafia, Kobayasiella, Envekadea, Stenoneis, Berkeleya, Climaconeis, and Parlibellus were compared with those of N. adminensis. Although each of these genera shows one or more similar features, none of them accommodates the salient morphological characteristics of N. adminensis. Therefore, a new genus, Sabbea gen. nov., is herein described, and Navicula adminensis is formally transferred to the new genus as Sabbea adminensis comb. nov. The genus Sabbea is characterized by uniseriate striae composed of small, rounded areolae occluded externally by individual hymenes, a rather simple raphe structure with straight, short proximal ends and short terminal raphe fissures, open girdle bands with double perforation and a very shallow mantle.

The effect of salinity on growth and amino acid composition in the marine diatom Nitzschia pungens

1992 ◽  
Vol 70 (11) ◽  
pp. 2198-2201 ◽  
Author(s):  
Anne E. Jackson ◽  
Stephen W. Ayer ◽  
Maurice V. Laycock
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Osmotic Pressure ◽  
Marine Diatom ◽  
Salinity Range ◽  
Optimal Salinity ◽  
Maritime Canada ◽  
Total Amino Acids ◽  
Positive Compound ◽  
Nitzschia Pungens

Nitzschia pungens f. multiseries and N. pungens f. pungens, isolated from two estuaries in Maritime Canada, were grown at various salinities (6–48‰) and their growth rates and free amino acid compositions were determined. The optimal salinity range for growth of f. multiseries was 30–45‰, whereas that of f. pungens was 15–30‰. At higher salinities there was increased production of a ninhydrin-positive compound, which was identified as the amino acid taurine. When f. multiseries was grown at a salinity of 48‰ and rapidly exposed to 15‰, the concentration of taurine decreased markedly (from 61 to 7% of the total amino acids). The evidence indicates that taurine may serve to regulate the osmotic pressure of N. pungens f. multiseries, a role not previously assigned to taurine in a marine diatom. Key words: Nitzschia pungens, salinity, osmotic pressure, amino acids, taurine.

scholarly journals Coxiella burnetiiblocks intracellular IL-17 signaling in macrophages

2018 ◽  
Author(s):  
Tatiana M. Clemente ◽  
Minal Mulye ◽  
Anna V. Justis ◽  
Srinivas Nallandhighal ◽  
Tuan M. Tran ◽  
...  
Keyword(s):  
Host Cell ◽  
Q Fever ◽  
Protective Role ◽  
Effector Proteins ◽  
Antimicrobial Protein ◽  
Dependent Manner ◽  
Cell Infection ◽  
Protein Levels ◽  
Host Cytoplasm ◽  
Infected Cells

AbstractCoxiella burnetiiis an obligate intracellular bacterium and the etiological agent of Q fever. Successful host cell infection requires theCoxiellaType IVB Secretion System (T4BSS), which translocates bacterial effector proteins across the vacuole membrane into the host cytoplasm, where they manipulate a variety of cell processes. To identify host cell targets ofCoxiellaT4BSS effector proteins, we determined the transcriptome of murine alveolar macrophages infected with aCoxiellaT4BSS effector mutant. We identified a set of inflammatory genes that are significantly upregulated in T4BSS mutant-infected cells compared to mock-infected cells or cells infected with wild type (WT) bacteria, suggestingCoxiellaT4BSS effector proteins downregulate expression of these genes. In addition, the IL-17 signaling pathway was identified as one of the top pathways affected by the bacteria. While previous studies demonstrated that IL-17 plays a protective role against several pathogens, the role of IL-17 duringCoxiellainfection is unknown. We found that IL-17 kills intracellularCoxiellain a dose-dependent manner, with the T4BSS mutant exhibiting significantly more sensitivity to IL-17 than WT bacteria. In addition, quantitative PCR confirmed increased expression of IL-17 downstream signaling genes in T4BSS mutant-infected cells compared to WT or mock-infected cells, including the pro-inflammatory cytokinesI11a, Il1bandTnfa, the chemokinesCxcl2andCcl5, and the antimicrobial proteinLcn2. We further confirmed that theCoxiellaT4BSS downregulates macrophage CXCL2/MIP-2 and CCL5/RANTES protein levels following IL-17 stimulation. Together, these data suggest thatCoxielladownregulates IL-17 signaling in a T4BSS-dependent manner in order to escape the macrophage immune response.

Phallus impudicus. [Descriptions of Fungi and Bacteria].

2022 ◽  
Author(s):  
D. W. Minter
Keyword(s):  
New York ◽  
North Carolina ◽  
South Carolina ◽  
Prince Edward Island ◽  
Conservation Status ◽  
District Of Columbia ◽  
Morphological Characteristics ◽  
Uttar Pradesh ◽  
Rio Grande ◽  
Jammu And Kashmir

Abstract A description is provided for Phallus impudicus. Some information on its morphological characteristics, associated organisms and substrata, dispersal and transmission, economic impacts, habitats and conservation status is given, along with details of its geographical distribution (Africa (Algeria, Liberia, Morocco, South Africa, Tanzania, Zimbabwe), Asi (Armenia, China, Anhui, Guangdong, Hainan, Hebei, Shanxi, Georgia, India, Assam, Jammu and Kashmir, Maharashtra, Uttar Pradesh, Iran, Israel, Japan, Kazakhstan, Kyrgyzstan, Malaysia, Myanmar, Nepal, Papua New Guinea, Russia, South Korea, Syria, Taiwan, Turkey, Vietnam), Atlantic Ocean (Spain, Islas Canarias), Australasia (Australia, Northern Territory, Queensland, New Zealand), Caribbean (Cuba, Jamaica, Puerto Rico), Central America (Costa Rica, Panama), Europe (Åland Islands, Andorra, Austria, Belarus, Belgium, Bulgaria, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Isle of Man, Italy, Jersey, Latvia, Lithuania, Luxembourg, Netherlands, Norway, Poland, Portugal, Romania, Russia, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Ukraine, UK), North America (Canada, Alberta, British Columbia, New Brunswick, Nova Scotia, Ontario, Prince Edward Island, Quebec, Saskatchewan, Mexico, USA, Alabama, Arizona, Arkansas, California, Colorado, Connecticut, Delaware, District of Columbia, Florida, Idaho, Illinois, Indiana, Iowa, Kansas, Kentucky, Maryland, Massachusetts, Michigan, Minnesota, Mississippi, Missouri, Nebraska, Nevada, New Hampshire, New Jersey, New Mexico, New York, North Carolina, North Dakota, Ohio, Oregon, Pennsylvania, South Carolina, South Dakota, Tennessee, Texas, Utah, Vermont, Virginia, Washington, Wisconsin), Pacific Ocean (Samoa), South America (Brazil, Amazonas, Paraíba, Rio Grande do Norte, Rio Grande do Sul, São Paulo, Chile, Colombia, Guyana, Uruguay)).

Pennate Diatom Nitzschia pungens as the Primary Source of Domoic Acid, a Toxin in Shellfish from Eastern Prince Edward Island, Canada

1989 ◽  
Vol 46 (7) ◽  
pp. 1203-1215 ◽  
Author(s):  
S. S. Bates ◽  
C J. Bird ◽  
A. S. W. de Freitas ◽  
R. Foxall ◽  
M. Gilgan ◽  
...  
Keyword(s):  
Prince Edward Island ◽  
Domoic Acid ◽  
Food Poisoning ◽  
Primary Source ◽  
Pennate Diatom ◽  
Closely Related Species ◽  
Plankton Bloom ◽  
Amphora Coffeaeformis ◽  
Human Poisoning ◽  
Nitzschia Pungens

An outbreak of food poisoning in Canada during autumn 1987 was traced to cultured blue mussels (Mytilus edulis) from the Cardigan Bay region of eastern Prince Edward Island (P.E.I.). The toxin, identified as domoic acid, had not previously been found in any shellfish and this outbreak represents the first known occurrence of human poisoning by this neurotoxin. A plankton bloom at the time of the outbreak consisted almost entirely of the pennate diatom, Nitzschia pungens f. multiseries, and a positive correlation was found between the number of N. pungens cells and the concentration of domoic acid in the plankton. Nitzschia pungens f. multiseries isolated from Cardigan Bay produced domoic acid in culture at levels (1 to 20 pg∙cell−1) comparable with values estimated for N. pungens in the plankton samples. Isolates of several Cardigan Bay phytoplankton, including the closely related species Nitzschia seriata, failed to produce domoic acid. Other Nitzschia spp. and two Amphora coffeaeformis isolates also failed to produce domoic acid. We conclude that N. pungens was the major source of the domoic acid in toxic mussels in eastern P.E.I. The recurrence, in November 1988, of a monospecific bloom of N. pungens and the presence of domoic acid in plankton and mussels reinforced this conclusion.

Trametes versicolor. [Descriptions of Fungi and Bacteria].

2022 ◽  
Author(s):  
D. W. Minter
Keyword(s):  
South Carolina ◽  
Rhode Island ◽  
Prince Edward Island ◽  
Trametes Versicolor ◽  
Tamil Nadu ◽  
Conservation Status ◽  
Morphological Characteristics ◽  
South Australia ◽  
Jammu And Kashmir ◽  
Mato Grosso

Abstract A description is provided for Trametes versicolor. Some information on morphological characteristics, its associated organisms and substrata, habitats, economic impacts, dispersal and transmission, conservation status is given, along with details of its geographical distribution (Africa (Benin, Burundi, Cameroon, Democratic Republic of the Congo, Ethiopia, Kenya, Libya, Malawi, Morocco, Nigeria, Rwanda, Sierra Leone, South Africa, Tanzania, Uganda, Zimbabwe.), Antarctica, Asia (Armenia, Azerbaijan, China, Anhui, Gansu, Guangdong, Guanxi, Guizhou, Hainan, Hebei, Heilongjang, Henan, Hong Kong, Hubei, Hunan, Jiangsu, Jiangxi, Jilin, Liaoning, Nei Mongol Autonomous Region, Qinghai, Shandong, Shanxi, Sichuan, Xinjiang, Yunnan, Zhejiang, Cyprus, Georgia, India, Assam, Goa, Himachal Pradesh, Jammu and Kashmir, Karnataka, Kerala, Madhya Pradesh, Maharashtra, Meghalaya, Punjab, Rajasthan, Sikkim, Tamil Nadu, Uttarakhand, West Bengal, Indonesia, Iran, Iraq, Japan, Kazakhstan (Almaty), Kyrgyzstan, Laos, Malaysia, Mongolia, Myanmar, Nepal, Pakistan, Philippines, Russia, Singapore, South Korea, Sri Lanka, Taiwan, Tajikistan, Thailand, Turkey, Turkmenistan, Uzbekistan, Vietnam, Atlantic Ocean (Bermuda), Australasia (Australia, Australian Capital Territory, New South Wales, Queensland, South Australia, Tasmania, Victoria, Western Australia, New Zealand), Caribbean (Cuba, Dominican Republic, Haiti, Jamaica, Puerto Rico), Central America (Belize, Costa Rica, El Salvador, Guatemala, Nicaragua, Panama), Europe (Andorra, Austria, Belarus, Belgium, Bosnia and Herzegovina, Bulgaria, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Isle of Man, Italy, Kosovo, Latvia, Lithuania, Luxembourg, Moldova, Netherlands, Norway, Poland, Portugal, Romania, Russia, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Ukraine, UK), Indian Ocean (Réunion), North America (Canada, Alberta, British Columbia, Manitoba, New Brunswick, Newfoundland and Labrador, Northwest Territories, Nova Scotia, Ontario, Prince Edward Island, Quebec, Saskatchewan, Yukon, Mexico, USA, Alabama, Alaska, Arizona, Arkansas, California, Colorado, Connecticut, Delaware, District of Columbia, Florida, Georgia, Idaho, Illinois, Indiana, Iowa, Kansas, Kentucky, Louisiana, Maine, Maryland, Massachusetts, Michigan, Minnesota, Mississippi, Missouri, Montana, Nebraska, Nevada, New Hampshire, New Jersey, New Mexico, New York, North Carolina, North Dakota, Ohio, Oklahoma, Oregon, Pennsylvania, Rhode Island, South Carolina, South Dakota, Tennessee, Texas, Utah, Vermont, Virginia, Washington, West Virginia, Wisconsin, Wyoming), Pacific Ocean (USA, Hawaii), South America (Argentina, Bolivia, Brazil, Acre, Amazonas, Bahia, Distrito Federal, Espírito Santo, Mato Grosso do Sul, Minas Gerais, Pará, Paraná, Pernambuco, Rio Grande do Sul, Rio de Janeiro, Rondônia, Roraima, Santa Catarina São Paulo, Chile, Colombia, Ecuador, Paraguay, Peru, Suriname, Uruguay, Venezuela))).

Capronia pilosella. [Descriptions of Fungi and Bacteria].

2021 ◽  
Author(s):  
P. F. Cannon
Keyword(s):  
New York ◽  
New Hampshire ◽  
Prince Edward Island ◽  
New South ◽  
Morphological Characteristics ◽  
South Australia ◽  
Australian Capital Territory ◽  
Rotten Wood ◽  
South Wales ◽  
Xinjiang Autonomous Region

Abstract A description is provided for Capronia pilosella, found on unidentified, very rotten wood. Some information on its morphological characteristics, associated organisms and substrata, dispersal and transmission, habitats and economic impacts is given, along with details of its geographical distribution (Asia (China, Xinjiang Autonomous Region, Taiwan), Australasia (Australia, Australian Capital Territory, New South Wales, Northern Territory, South Australia, Tasmania, Victoria, New Zealand), Europe (Austria, Belgium, Croatia, Czech Republic, Denmark, Finland, France, Germany, Ireland, Netherlands, Norway, Spain, Sweden, Switzerland, Ukraine, UK), North America (Canada, Ontario, Prince Edward Island, USA, Idaho, Indiana, Iowa, Missouri, New Hampshire, New Jersey, New York), Pacific Ocean (Fiji), South America (Argentina, Brazil, Amazonas)).

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