Inheritance of aspartate aminotransferase (AAT) in Cucumis species as revealed by interspecific hybridization

2006 ◽  
Vol 84 (9) ◽  
pp. 1503-1507 ◽  
Author(s):  
Jin-Feng Chen ◽  
Gang Ren ◽  
Xiang-Dong Luo ◽  
Jack Staub ◽  
Molly M. Jahn
Keyword(s):  
Cucumis Sativus ◽  
Aspartate Aminotransferase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Staining Intensity ◽  
Recurrent Parent ◽  
Cucumis Sativus L ◽  
Dosage Effects ◽  
Cucumis Species ◽  
Single Allele ◽  
The Difference

The inheritance of aspartate aminotransferase (AAT) isozymes was investigated in Cucumis sativus L. (CC; 2n = 2x = 14), Cucumis hystrix Chakr. (HH; 2n = 2x = 24), the synthetic amphidiploid species C. hytivus Chen & Kirkbride (HHCC; 2n = 4x = 38), and the allotriploid (HCC; 2n = 3x = 26) from backcrossing C. hytivus to C. sativus. Two polymorphic loci, Aat-1 and Aat-2, and one monomorphic locus, Aat-3, were detected among these parents and their progenies by using polyacrylamide gel electrophoresis. Cucumis sativus displayed the fast-migrating anodic band for Aat-1 (2), while C. hystrix contained a slow-migrating cathodic band (1). For Aat-2, the slow-migrating cathodic band was observed with C. sativus (1), whereas C. hystrix contained the fast-migrating anodic band (2). Cucumis hytivus, a synthetic species derived from doubling the chromosome number of a F1 from a C. sativus × C. hystrix mating, exhibited the typical hybrid dimeric banding pattern resulting from the combination of two parental homomeric products with equal staining intensity and a heteromeric product with intermediate mobility and greater staining intensity than either homomeric product. The difference in band intensity between C. hytivus and its backcross progenies, when C. sativus was the recurrent parent, were due to the dosage effects of alleles at Aat-1 and Aat-2. These banding morphotypes can be used for typing of C. hytivus and BC1 progenies that are similar in morphology. Aat-3 was monomorphic in this mating, encoding a single allele Aat-3 (1).

scholarly journals The effect of ammonium ions on the activity of glutamate dehydrogenase, alanine aminotransferase and aspartate aminotransferase in Cucumis sativus L. seedlings

2014 ◽  
Vol 50 (3) ◽  
pp. 429-436
Author(s):  
Genowefa Kubiak-Dobosz
Keyword(s):  
Glutamate Dehydrogenase ◽  
Cucumis Sativus ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Ammonium Ions ◽  
Cucumis Sativus L ◽  
Factors Affecting ◽  
Cucumber Seedlings ◽  
Young Leaves ◽  
Medium Activity

Changes in the activity of glutamate dehydrogenase (GDH), alanine aminotransferase (GPT) and aspartate aminotransferase (GOT) were studied in various organs of <em>Cucumis sativus</em> L. seedlings in relation to the uptake of mineral nitrogen (in form of N0<sub>3</sub><sup>-</sup> or NH<sub>4</sub><sup>+</sup> ) from the medium. Activity of GDH, GPT, and GOT was higher in young leaves and roots of cucumber seedlings if the plants developed- in an ammonium medium. No similar changes of aminotransferases activity were noted in the cotyledons. Factors affecting varying effect of ammonium ions upon GPT and GOT activity are discussed for particular organs of cucumber seedlings.

scholarly journals Influence of light and mineral nitrogen forms on the activity of some enzymes in Cucumis sativus L. cotyledons

2015 ◽  
Vol 48 (3) ◽  
pp. 443-452 ◽  
Author(s):  
G. Kubik-Dobosz ◽  
K. Soroka
Keyword(s):  
Amino Acids ◽  
Glutamine Synthetase ◽  
Glutamate Dehydrogenase ◽  
Cucumis Sativus ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Glutamate Synthase ◽  
Mineral Nitrogen ◽  
Nitrogen Forms ◽  
Cucumis Sativus L

It was demonstrated that when nitrogen was deficient in the medium, the activity of glutamine synthetase (GS), glutamate dehydrogenase (GDH), alanine aminotransferase (GPT) and aspartate aminotransferase (GOT) in etiolated cucumber cotyledons was higher than in those of seedlings growing under light. When the plants grew on nitrate or ammonium medium, light stimulated GS activity and depressed that of GDH and GOT, without changing the activity of GPT. It was found that the influence of the form of mineral nitrogen on the activity of the studied enzymes was dependent on light. On the basis of the results. obtained, the contribution of the GS glutamate synthase system and GDH to the incorporation of the taken up nitrogen into the amino acids in light and in darkness is discussed.

scholarly journals Effect of Chilling on the Gravitropic Response of `Poinsett 76' Cucumber (Cucumis sativus L.) Radicles

1996 ◽  
Vol 121 (5) ◽  
pp. 901-907 ◽  
Author(s):  
Abdur Rab ◽  
Mikal E. Saltveit
Keyword(s):  
Heat Shock ◽  
Cucumis Sativus ◽  
Horizontal Position ◽  
Cucumis Sativus L ◽  
Gravitropic Response ◽  
Radicle Elongation ◽  
The Difference ◽  
Germinated Seeds

Germinating `Poinsett 76' cucumber (Cucumis sativus) seeds are chilling sensitive, and subsequent radicle elongation is inhibited by exposure to nonfreezing temperatures below 10 °C. Reorienting germinated seeds with 5-mm-long radicles from a vertical to a horizontal position induced gravitropic curvature within 2 hours at 10 to 25 °C without significantly affecting the rate of radicle elongation. However, neither elongation nor curvature occurred in similar seeds held at 2.5 or 5 °C. Chilling seeds with 5-mm-long radicles at 2.5 °C for 18 hours significantly reduced the subsequent rate of radicle elongation at 25 °C by 47% compared with nonchilled control, while gravitropic curvature was reduced by only 34%. After 36 hours of recovery at 25 °C, the difference was even more pronounced; elongation was reduced by 26% while curvature was reduced by only 6%. Clearly, gravitropic curvature was less chilling sensitive than radicle elongation, despite the fact that differential elongation of cells in the radicle are needed to produce curvature. Exposing seeds with 5-mm-long radicles to a heat shock of 45 °C for 4 to 10 minutes significantly diminished the chilling-induced reduction in radicle elongation and gravitropic curvature.

scholarly journals Electrophoretic Banding Pattern Of Esterase Isozyme In Different Tissues Of Puntius Sophore (Cyprinidae : Cypriniformes)

2016 ◽  
Vol 42 (2) ◽  
pp. 201-208
Author(s):  
Hawa Jahan ◽  
Partha Sarathi Gope ◽  
Mohammad Shamimul Alam ◽  
Reza Md Shahjahan
Keyword(s):  
Skeletal Muscles ◽  
Polyacrylamide Gel Electrophoresis ◽  
Staining Intensity ◽  
Reproductive Organ ◽  
Local Market ◽  
Esterase Isozyme ◽  
Single Allele ◽  
Hind Gut ◽  
Different Tissues ◽  
Puntius Sophore

Esterase isozyme of different tissues of Puntius sophore was analyzed using 7.5 % polyacrylamide gel electrophoresis (PAGE). Fish specimens were collected from local market. The tissues used were taken from different muscles, stomach, fore-, mid- and hind-gut, liver, eyes, gill, heart, kidney, fore-, mid- and hind- brain, eggs and ovary. Six different esterase bands were detected, named Est-1, Est-2, Est-3, Est-4, Est-5 and Est-6 and their relative mobility were 1.0, 0.84, 0.62, 0.33, 0.26 and 0.13 respectively, each of them representing a single allele. The highest esterase activity was found in liver, followed by gill, kidney, heart, brain, intestine, stomach, eye, reproductive organ and skeletal muscles as detected in the staining intensity. Staining intensity of Est-4 and Est-5 was higher and Est-6 was the least stained in all the tissues. Asiat. Soc. Bangladesh, Sci. 42(2): 201-208, December 2016

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