Sucrose synthase isozyme SUS1 in the maize root cap is preferentially localized in the endopolyploid outer cells

2004 ◽  
Vol 82 (1) ◽  
pp. 96-103 ◽  
Author(s):  
Aleš Kladnik ◽  
Barbara Vilhar ◽  
Prem S Chourey ◽  
Marina Dermastia
Keyword(s):  
Zea Mays ◽  
Sucrose Synthase ◽  
Nuclear Dna ◽  
Zea Mays L ◽  
Nuclear Dna Content ◽  
Image Cytometry ◽  
Root Cap ◽  
Dna Amount ◽  
The Relationship ◽  
Endopolyploid Cells

The structure of the maize (Zea mays L.) root cap was studied to quantitatively evaluate the relationship among the size of the cells, their endopolyploidy level, and the abundance of the sucrose synthase isozyme SUS1. Median longitudinal root cap sections were analysed using immunolocalization, quantitative DNA staining, and image cytometry. Both the immunolocalization signal for the SUS1 protein and the endopolyploidy level increased from calyptrogen towards the root cap periphery and were thus the highest in the outer cells. These cells had a nuclear DNA content of mostly 8C or higher and the largest volumes of all root cap cells. The high amount of SUS1 protein in the outer, endopolyploid cells suggests an association between endoreduplication and the abundance of this enzyme. The outer cells are involved in mucilage production; hence, there is a possibility that sucrose synthase provides monosaccharide precursors for mucilage synthesis.Key words: nuclear DNA amount, endoreduplication, immunolocalization, image cytometry, Zea mays L.

scholarly journals Mitotic activity and changes in DNA content during Zea mays L. endosperm development

2014 ◽  
Vol 71 (3) ◽  
pp. 195-200
Author(s):  
Hanna Kuran ◽  
Kazimierz Marciniak
Keyword(s):  
Zea Mays ◽  
Mitotic Activity ◽  
Dna Content ◽  
Nuclear Dna ◽  
Zea Mays L ◽  
Nuclear Dna Content ◽  
Staining Intensity ◽  
Endosperm Development ◽  
Dna Contents ◽  
Mitotic Figures

Mitotic activity and nuclear DNA content in endosperm of <em>Zea mays</em> cv. Złota Karłowa were examined. DNA content was cytophotometrically measured on squashed preparations after Feulgen procedure. Mitotic activity in endosperm was determined till the stage when embryo sack reached 4.5 mm in length. Some of mitotic figures show multiplied DNA content. Endosperm nuclei have various DNA contents which increase throughout endosperm development. DNA content enhancement indicates endoreduplication in progress. Some nuclei with high DNA content display changes in chromatin structure, which are expressed by the presence of strands and aggregates of chromatin characterised by high staining intensity. A conclusion has been drawn that mitotic divisions and the endoreduplication phase of nuclear DNA may occur simultaneously and dominate one over another at different phases of endosperm development.

Inheritance of nuclear DNA content in leaf epidermal cells of Zea mays L.

1997 ◽  
Vol 94 (6-7) ◽  
pp. 782-787 ◽  
Author(s):  
A Cavallini ◽  
L. Natali ◽  
G. Cionini ◽  
C. Balconi ◽  
F. D’Amato
Keyword(s):  
Zea Mays ◽  
Dna Content ◽  
Nuclear Dna ◽  
Zea Mays L ◽  
Nuclear Dna Content ◽  
Epidermal Cells

Modelling of the root cap of Zea mays L in relation to temperature

1988 ◽  
Vol 108 (3) ◽  
pp. 259-262 ◽  
Author(s):  
F. A. L. CLOWES ◽  
R. WADEKAR
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Root Cap

Preparation of rat epididymal α-l-fucosidase free from other glycosidases: its action on root-cap slime from Zea mays L

1976 ◽  
Vol 47 (1) ◽  
pp. 141-150 ◽  
Author(s):  
Kenneth Wright ◽  
D.H. Northcote ◽  
Robin M. Davey
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Root Cap

The cell cycle and its relationship to development in Acanthamoeba castellanii

1987 ◽  
Vol 88 (5) ◽  
pp. 579-590
Author(s):  
MICHAEL STÖHR ◽  
KURT BOMMERT ◽  
INGRID SCHULZE ◽  
HELGA JANTZEN
Keyword(s):  
Cell Cycle ◽  
Stationary Phase ◽  
Nuclear Dna ◽  
Acanthamoeba Castellanii ◽  
Nuclear Dna Content ◽  
Defined Medium ◽  
G2 Phase ◽  
D Cells ◽  
High Degree ◽  
The Relationship

The cell cycle and the relationship between particular cell cycle phases and the differentiation of trophozoites into cysts were reinvestigated in Acanthamoeba castellanii using flow fluorometric measurements of nuclear DNA content and synthesis and synchronization of cells by release from the stationary phase. The investigation was performed with cultures growing in non-defined medium (ND cells) showing a high degree of encystation in response to starvation and with subcultures growing in chemically defined nutrient medium (D cells) exhibiting a very low encystation competence. In both cultures the cell cycle starts with a short S phase taking place simultaneously with cytokinesis followed by a long G2 phase. A G1 phase seems to be either absent or very short. Synchronization experiments reveal that in ND cells encystation is initiated from a particular position of late G2. The high encystation competence of stationary phase ND cells seems to be due to arrest of cells at this particular cell cycle position. The lack of encystation competence of stationary phase D cells correlates with the loss of accumulation of cells at this particular stage of the cell cycle. This change of the property of cells is related to the growth condition and not to an irreversible loss of encystation competence of D cells.

scholarly journals Structure of the sucrose synthase gene on chromosome 9 of Zea mays L

1985 ◽  
Vol 4 (6) ◽  
pp. 1373-1380 ◽  
Author(s):  
W. Werr ◽  
W. -B. Frommer ◽  
C. Maas ◽  
P. Starlinger
Keyword(s):  
Zea Mays ◽  
Sucrose Synthase ◽  
Zea Mays L ◽  
Chromosome 9

DNA content in Tradescantia

1985 ◽  
Vol 27 (6) ◽  
pp. 766-775 ◽  
Author(s):  
Arturo Martínez ◽  
Héctor D. Ginzo
Keyword(s):  
North America ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Life Forms ◽  
Chromosome Size ◽  
Polyploid Complex ◽  
Ploidy Levels ◽  
Vegetative Adaptation ◽  
The Relationship

There is a wide variation in the nuclear DNA content and chromosome size between the species belonging to the T. crassifolia and T. virginiana alliances (all the species but one are native to Central and North America). Also the DNA content per genome decreases when the ploidy level increases within the same specific polyploid complex with three ploidy levels (2x, 4x, and 6x). In contrast, no variation was found in the DNA content per genome between different ploidy levels in the T. fluminensis alliance (all the species are native to South America) where they range from 6x to 22x. Since all the species described here are perennials with various life forms, it was possible to analyze the relationship between the DNA content and their vegetative adaptation to the environment. The more specialized species (geophytes and hemicryptophytes) have a higher amount of DNA than the chamaephytes adapted to live in relatively more mesic regions. In the species living in Central and North America there is a positive correlation between the increase in DNA content and the latitude of their native regions.Key words: Tradescantia, DNA content, geographical distribution, life forms, polyploidy.

Nuclear DNA content of three Eucalyptus species estimated by flow and image cytometry

2009 ◽  
Vol 57 (6) ◽  
pp. 524 ◽  
Author(s):  
Milene Miranda Praça ◽  
Carlos Roberto Carvalho ◽  
Carolina Ribeiro Diniz Boaventura Novaes
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Methodological Approach ◽  
Nuclear Dna Content ◽  
Image Cytometry ◽  
Eucalyptus Species ◽  
International Consensus ◽  
Mean Values ◽  
Dna Contents ◽  
Nuclear Dna Contents

Previous flow cytometry (FCM) analyses delivered nearly equal mean values of nuclear 2C DNA content for Eucalyptus grandis Hill ex Maiden and E. urophylla S. T. Blake (1.33 pg and 1.34 pg, respectively), whereas E. globulus Labill. presented distinct mean values (1.09, 1.13 and 1.40). These differences have been attributed to the different methodological approach, utilised plant cultivar and presence of intrinsic metabolic compounds that affect fluorochrome fluorescence. In the present study, a FCM and image cytometry (ICM) design, following international consensus criteria, were adopted to reassess the nuclear DNA contents of the above-mentioned Eucalyptus species. Statistical analyses revealed either similar or discrepant nuclear DNA contents, depending on the standard species used and whether FCM or ICM was employed. Our results demonstrated that 2C DNA values obtained by FCM and ICM were most uniform when Solanum lycopersicum was used as a standard. Moreover, the values obtained for E. grandis and E. urophylla were close, but differed as much as 24.63% in relation to previous data, and E. globulus proportionally varied up to 25%. New DNA content values are suggested for these eucalypt species.

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