Inheritance of nuclear DNA content in leaf epidermal cells of Zea mays L.

1997 ◽  
Vol 94 (6-7) ◽  
pp. 782-787 ◽  
Author(s):  
A Cavallini ◽  
L. Natali ◽  
G. Cionini ◽  
C. Balconi ◽  
F. D’Amato
2014 ◽  
Vol 71 (3) ◽  
pp. 195-200
Author(s):  
Hanna Kuran ◽  
Kazimierz Marciniak

Mitotic activity and nuclear DNA content in endosperm of <em>Zea mays</em> cv. Złota Karłowa were examined. DNA content was cytophotometrically measured on squashed preparations after Feulgen procedure. Mitotic activity in endosperm was determined till the stage when embryo sack reached 4.5 mm in length. Some of mitotic figures show multiplied DNA content. Endosperm nuclei have various DNA contents which increase throughout endosperm development. DNA content enhancement indicates endoreduplication in progress. Some nuclei with high DNA content display changes in chromatin structure, which are expressed by the presence of strands and aggregates of chromatin characterised by high staining intensity. A conclusion has been drawn that mitotic divisions and the endoreduplication phase of nuclear DNA may occur simultaneously and dominate one over another at different phases of endosperm development.


Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 723-729 ◽  
Author(s):  
Lidia Poggio ◽  
Marcela Rosato ◽  
Carlos A. Naranjo

The cytoplasm of Zea mays ssp. mexicana (teosinte) affects several inherited traits when combined with genotypes of maize (Zea mays ssp. mays). The meiotic behavior and the total DNA content of four lines of maize with teosinte cytoplasm were compared with those of the parental lines. The results obtained suggest that the cytoplasm of teosinte promotes an increase in total nuclear DNA content, perhaps through an increase of highly repetitive DNA in the knob zones. The analysis of meiotic behavior indicates that the cytoplasm of teosinte can alter the spatial distribution of the genomes, since two groups of five bivalents each were observed at a high frequency. During prophase I – anaphase I, each group of five bivalents behaves in a slightly asynchronous way with respect to the other group and, moreover, two nucleoli were observed in 10% of the cells. These results suggest that the cytoplasm of teosinte could induce changes affecting genomic structure and function in some maize genotypes. These changes are of potential importance for breeding programs and evolutionary studies.Key words: maize, teosinte, Zea mays ssp. mexicana, alloplasmy, nuclear-cytoplasmic interaction, meiotic behavior, DNA content.


2004 ◽  
Vol 82 (1) ◽  
pp. 96-103 ◽  
Author(s):  
Aleš Kladnik ◽  
Barbara Vilhar ◽  
Prem S Chourey ◽  
Marina Dermastia

The structure of the maize (Zea mays L.) root cap was studied to quantitatively evaluate the relationship among the size of the cells, their endopolyploidy level, and the abundance of the sucrose synthase isozyme SUS1. Median longitudinal root cap sections were analysed using immunolocalization, quantitative DNA staining, and image cytometry. Both the immunolocalization signal for the SUS1 protein and the endopolyploidy level increased from calyptrogen towards the root cap periphery and were thus the highest in the outer cells. These cells had a nuclear DNA content of mostly 8C or higher and the largest volumes of all root cap cells. The high amount of SUS1 protein in the outer, endopolyploid cells suggests an association between endoreduplication and the abundance of this enzyme. The outer cells are involved in mucilage production; hence, there is a possibility that sucrose synthase provides monosaccharide precursors for mucilage synthesis.Key words: nuclear DNA amount, endoreduplication, immunolocalization, image cytometry, Zea mays L.


1988 ◽  
Vol 12 (4) ◽  
pp. 503-507 ◽  
Author(s):  
Jaap F. Hamming ◽  
Lodewijk J. D. M. Schelfhout ◽  
Cees J. Cornelisse ◽  
Cornelis J. H. van de Velde ◽  
Bernard M. Goslings ◽  
...  

Author(s):  
Terrence R. Tiersch ◽  
Robert W. Chandler ◽  
Klaus D. Kallman ◽  
Stephen S. Wachtel

2006 ◽  
Vol 107 (2) ◽  
pp. 211-221 ◽  
Author(s):  
Mieczysław Kuraś ◽  
Julita Nowakowska ◽  
Elwira Śliwińska ◽  
Radosław Pilarski ◽  
Renata Ilasz ◽  
...  

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