Effects of altitude on tracheid differentiation and lignification of Norway spruce

2001 ◽  
Vol 79 (7) ◽  
pp. 815-821 ◽  
Author(s):  
W Gindl ◽  
M Grabner ◽  
R Wimmer
Keyword(s):  
Norway Spruce ◽  
Cell Walls ◽  
Lignin Content ◽  
Single Cells ◽  
Rank Correlation ◽  
Wood Properties ◽  
Wood Formation ◽  
High Elevation ◽  
Ultraviolet Microscopy ◽  
Tracheid Differentiation

The effect of altitude on lignification is important in developing an understanding of what drives natural variation in wood properties. Cambia of two Norway spruce trees, growing at altitudes of 580 and 1260 m a.s.l., were periodically sampled to measure tracheid dimensions and cellular lignin content. The low-elevation tree showed a higher rate of cell division with thicker cell walls and wider growth rings. The maturation phase of tracheids at high elevation was completed by the end of October while low elevation latewood tracheids were still lignifying. As revealed by ultraviolet microscopy, lignin content in single cells, as well as in complete tree-rings, was higher at high elevation. Rank correlation analysis indicated high negative correlation between lignin content of tracheid cell walls and corresponding wall thickness. It is hypothesized that trees growing at higher altitudes compensate for the thinner cell walls with an increased lignin content which helps to maintain mechanical integrity of the xylem.Key words: altitude, lignin, tracheid, wood formation, ultraviolet microscopy.

Lignification of spruce tracheid secondary cell walls related to longitudinal hardness and modulus of elasticity using nano-indentation

2002 ◽  
Vol 80 (10) ◽  
pp. 1029-1033 ◽  
Author(s):  
W Gindl ◽  
H S Gupta ◽  
C Grünwald
Keyword(s):  
Cell Wall ◽  
Modulus Of Elasticity ◽  
Cell Walls ◽  
Secondary Cell Wall ◽  
Lignin Content ◽  
Wood Formation ◽  
Nano Indentation ◽  
Secondary Cell Walls ◽  
Cell Wall Development ◽  
The One

The lignin content and the mechanical properties of lignifying and fully lignified spruce tracheid secondary cell walls were determined using UV microscopy and nano-indentation, respectively. The average lignin content of developing tracheids was 0.10 g·g–1, as compared with 0.21 g·g–1 in mature tracheids. The modulus of elasticity of developing cells was on average 22% lower than the one measured in mature, fully lignified cells. For the longitudinal hardness, a larger difference of 26% was observed. As lignifying cells in the cambial zone are undergoing cell wall development, spaces in the cellulose–hemicellulose structure are filled with lignin and the density of the cell wall is believed to increase. It is therefore suggested that the observed difference in modulus of elasticity between developing and fully lignified cell walls is due to the filling of spaces with lignin and an increase of the packing density of the cell wall during lignification. Although remarkably less stiff than the composite polysaccharide structure in the secondary cell wall, lignin may be considered equally hard. Therefore, the observed increase in lignin content may contribute directly to the measured increase of hardness.Key words: secondary cell wall, hardness, lignin, modulus of elasticity, wood formation.

Susceptibility of pretreated wood sections of Norway spruce (Picea abies) clones to enzymatic hydrolysis

2012 ◽  
Vol 42 (1) ◽  
pp. 38-46 ◽  
Author(s):  
Yohama Puentes Rodriguez ◽  
Helena Puhakka-Tarvainen ◽  
Ossi Pastinen ◽  
Matti Siika-aho ◽  
Leila Alvila ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Picea Abies ◽  
Norway Spruce ◽  
Wood Density ◽  
Lignin Content ◽  
Trichoderma Viride ◽  
Wood Decay ◽  
Wood Properties ◽  
Acid Pretreatment ◽  
The Relationship

The structure of softwoods, which confers resistance to degradation through hydrolysis and decay, currently limits their use for the production of biofuels. However, since wood is very heterogeneous, it is possible that differences in wood properties within and between trees could differentially affect its processability. In this research, heartwood (inner) and sapwood (outer) from Norway spruce ( Picea abies (L.) Karst.) clones were enzymatically hydrolyzed by Trichoderma viride cellulases after concentrated acid pretreatment. Wood sections with two particle sizes were compared based on their susceptibility to enzymatic hydrolysis, evaluated by assaying the formation of hydrolysis products and measured as reducing sugar yield (RSY). We also studied the relationship between RSY and the susceptibility to Heterobasidion parviporum wood decay and whether these traits are reflected in wood density and yield. Wood from the outer section produced more RSY with higher glucan but lower lignin content than wood from the inner section. Furthermore, susceptibility to enzymatic hydrolysis was positively correlated with H. parviporum wood decay, while both processes were negatively correlated with wood density. Our results revealed the importance of clonal trials for identifying suitable lignocellulosic biomass when considering wood properties and indicate that potential genotypes for the production of biofuels are not necessarily the most productive.

Silicification of wood-cell walls

1994 ◽  
Vol 52 ◽  
pp. 428-429
Author(s):  
S. E. Keckler ◽  
D. M. Dabbs ◽  
N. Yao ◽  
I. A. Aksay
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Cell Walls ◽  
Lignin Content ◽  
Resin Composite ◽  
Middle Lamella ◽  
Cellulose Fibers ◽  
Complex Structures ◽  
Rich Region ◽  
Inorganic Precursors

Cellular organic structures such as wood can be used as scaffolds for the synthesis of complex structures of organic/ceramic nanocomposites. The wood cell is a fiber-reinforced resin composite of cellulose fibers in a lignin matrix. A single cell wall, containing several layers of different fiber orientations and lignin content, is separated from its neighboring wall by the middle lamella, a lignin-rich region. In order to achieve total mineralization, deposition on and in the cell wall must be achieved. Geological fossilization of wood occurs as permineralization (filling the void spaces with mineral) and petrifaction (mineralizing the cell wall as the organic component decays) through infiltration of wood with inorganics after growth. Conversely, living plants can incorporate inorganics into their cells and in some cases into the cell walls during growth. In a recent study, we mimicked geological fossilization by infiltrating inorganic precursors into wood cells in order to enhance the properties of wood. In the current work, we use electron microscopy to examine the structure of silica formed in the cell walls after infiltration of tetraethoxysilane (TEOS).

scholarly journals Involvement of CesA4, CesA7-A/B and CesA8-A/B in secondary wall formation in Populus trichocarpa wood

2019 ◽  
Vol 40 (1) ◽  
pp. 73-89 ◽  
Author(s):  
Manzar Abbas ◽  
Ilona Peszlen ◽  
Rui Shi ◽  
Hoon Kim ◽  
Rui Katahira ◽  
...  
Keyword(s):  
Solid State ◽  
Mechanical Strength ◽  
Cell Walls ◽  
Secondary Wall ◽  
Populus Trichocarpa ◽  
Wood Formation ◽  
Fiber Cell ◽  
Modulus Of Rupture ◽  
Cellulose Content ◽  
Wall Formation

Abstract Cellulose synthase A genes (CesAs) are responsible for cellulose biosynthesis in plant cell walls. In this study, functions of secondary wall cellulose synthases PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B were characterized during wood formation in Populus trichocarpa (Torr. & Gray). CesA RNAi knockdown transgenic plants exhibited stunted growth, narrow leaves, early necrosis, reduced stature, collapsed vessels, thinner fiber cell walls and extended fiber lumen diameters. In the RNAi knockdown transgenics, stems exhibited reduced mechanical strength, with reduced modulus of rupture (MOR) and modulus of elasticity (MOE). The reduced mechanical strength may be due to thinner fiber cell walls. Vessels in the xylem of the transgenics were collapsed, indicating that water transport in xylem may be affected and thus causing early necrosis in leaves. A dramatic decrease in cellulose content was observed in the RNAi knockdown transgenics. Compared with wildtype, the cellulose content was significantly decreased in the PtrCesA4, PtrCesA7 and PtrCesA8 RNAi knockdown transgenics. As a result, lignin and xylem contents were proportionally increased. The wood composition changes were confirmed by solid-state NMR, two-dimensional solution-state NMR and sum-frequency-generation vibration (SFG) analyses. Both solid-state nuclear magnetic resonance (NMR) and SFG analyses demonstrated that knockdown of PtrCesAs did not affect cellulose crystallinity index. Our results provided the evidence for the involvement of PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B in secondary cell wall formation in wood and demonstrated the pleiotropic effects of their perturbations on wood formation.

Grade yields and wood properties of Norway spruce [Picea abies (L.) Karst.] from the Maritimes

1995 ◽  
Vol 71 (4) ◽  
pp. 473-478
Author(s):  
Y. H. Chui
Keyword(s):  
Mechanical Properties ◽  
Picea Abies ◽  
Norway Spruce ◽  
Wood Quality ◽  
Wood Properties ◽  
Bending Properties ◽  
Clear Wood ◽  
Comparative Information ◽  
Grade Yield

Norway spruce [Picea abies (L.) Karst.] is one of the major non-native softwood species in the Maritimes. A project was undertaken to evaluate the grade yields and mechanical properties of Norway spruce. The project also provided comparative information on the wood quality of two Norway spruce provenances from Germany and Poland. Four plantations were selected for the study with two of these plantations containing trees of known provenances. One plantation was mature and the other three were juvenile. In total, 530 pieces of lumber and the same number of matched small clear specimens were tested for bending properties. Prior to testing, the lumber was visually graded according to both British and Canadian specifications. Quality of lumber varied significantly between sites. Lumber from the Polish provenance had slightly better mechanical properties than that from trees of the German provenance. Compared with published information, the plantation-grown Norway spruce had lower clear wood bending properties and specific gravity than primary eastern Canadian spruce species and balsam fir, and natural Norway spruce grown in Europe. Key words: Norway spruce [Picea abies (L.) Karst.], wood quality, bending properties, grade yield

Negative gravitropism of Ginkgo biloba: growth stress and reaction wood formation

Holzforschung ◽  
2016 ◽  
Vol 70 (3) ◽  
pp. 267-274 ◽  
Author(s):  
Tatsuya Shirai ◽  
Hiroyuki Yamamoto ◽  
Miyuki Matsuo ◽  
Mikuri Inatsugu ◽  
Masato Yoshida ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
Lignin Content ◽  
Linear Regression Analysis ◽  
Multiple Linear Regression Analysis ◽  
Wood Formation ◽  
Growth Stress ◽  
Reaction Wood ◽  
Cellulose Content ◽  
S2 Layer ◽  
Negative Gravitropism

Abstract Ginkgo (Ginkgo biloba L.) forms thick, lignified secondary xylem in the cylindrical stem as in Pinales (commonly called conifers), although it has more phylogenetic affinity to Cycadales than to conifers. Ginkgo forms compression wood-like (CW-like) reaction wood (RW) in its inclined stem as it is the case in conifers. However, the distribution of growth stress is not yet investigated in the RW of ginkgo, and thus this tissue resulting from negative gravitropism is still waiting for closer consideration. The present study intended to fill this gap. It has been demonstrated that, indeed, ginkgo forms RW tissue on the lower side of the inclined stem, where the compressive growth stress (CGS) was generated. In the RW, the micorofibril angle in the S2 layer, the air-dried density, and the lignin content increased, whereas the cellulose content decreased. These data are quite similar to those of conifer CWs. The multiple linear regression analysis revealed that the CGS is significantly correlated by the changes in the aforementioned parameters. It can be safely concluded that the negative gravitropism of ginkgo is very similar to that of conifers.

scholarly journals Patatin-Related Phospholipase AtpPLAIIIα Affects Lignification of Xylem in Arabidopsis and Hybrid Poplars

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 451 ◽  
Author(s):  
Jin Hoon Jang ◽  
Ok Ran Lee
Keyword(s):  
Cell Walls ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Biofuel Production ◽  
Amide Bonds ◽  
Hybrid Poplars ◽  
Plant Lipids ◽  
Lipid Acyl Hydrolase ◽  
Specific Regulation

Lipid acyl hydrolase are a diverse group of enzymes that hydrolyze the ester or amide bonds of fatty acid in plant lipids. Patatin-related phospholipase AIIIs (pPLAIIIs) are one of major lipid acyl hydrolases that are less closely related to potato tuber patatins and are plant-specific. Recently, overexpression of ginseng-derived PgpPLAIIIβ was reported to be involved in the reduced level of lignin content in Arabidopsis and the mature xylem layer of poplar. The presence of lignin-polysaccharides renders cell walls recalcitrant for pulping and biofuel production. The tissue-specific regulation of lignin biosynthesis, without altering all xylem in plants, can be utilized usefully by keeping mechanical strength and resistance to various environmental stimuli. To identify another pPLAIII homolog from Arabidopsis, constitutively overexpressed AtpPLAIIIα was characterized for xylem lignification in two well-studied model plants, Arabidopsis and poplar. The characterization of gene function in annual and perennial plants with respect to lignin biosynthesis revealed the functional redundancy of less lignification via downregulation of lignin biosynthesis-related genes.

The effects of thermal treatment on the nanomechanical behavior of bamboo (Phyllostachys pubescens Mazel ex H. de Lehaie) cell walls observed by nanoindentation, XRD, and wet chemistry

Holzforschung ◽  
2017 ◽  
Vol 71 (2) ◽  
pp. 129-135 ◽  
Author(s):  
Yanjun Li ◽  
Chengjian Huang ◽  
Li Wang ◽  
Siqun Wang ◽  
Xinzhou Wang
Keyword(s):  
Cell Wall ◽  
Thermal Treatment ◽  
Cell Walls ◽  
Treatment Time ◽  
Lignin Content ◽  
Phyllostachys Pubescens ◽  
X Ray Diffraction ◽  
Wet Chemistry ◽  
X Ray ◽  
Wet Chemical

Abstract The effects of thermal treatment of bamboo at 130, 150, 170, and 190°C for 2, 4, and 6 h were investigated in terms of changes in chemical composition, cellulose crystallinity, and mechanical behavior of the cell-wall level by means of wet chemical analysis, X-ray diffraction (XRD), and nanoindentation (NI). Particularly, the reduced elastic modulus (Er), hardness (H), and creep behavior were in focus. Both the temperature and treatment time showed significant effects. Expectedly, the hemicelluloses were degraded and the relative lignin content was elevated, while the crystallinity of the cellulose moiety was increased upon thermal treatment. The Er and H data of the cell wall were increased after 6 h treatment at 190°C, from 18.4 to 22.0 GPa and from 0.45 to 0.65 GPa, respectively. The thermal treatment led to a decrease of the creep ratio (CIT) under the same conditions by ca. 28%. The indentation strain state (εi) also decreased significantly after thermal treatment during the load-holding stage.

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