Armillaria sinapina in herbaceous plant material from a peatland in Alberta, Canada

2001 ◽  
Vol 79 (5) ◽  
pp. 643-647 ◽  
Author(s):  
Markus N Thormann ◽  
Colin L Myrholm ◽  
Ken I Mallett

Armillaria sinapina Bérubé & Dessureault mycelium was isolated once from living Carex aquatilis Wahlenb. rhizomes, twice from decomposing C. aquatilis leaves, and three times from decomposing Salix planifolia Pursh leaves in a southern boreal sedge-dominated fen in Alberta, Canada. Restriction fragment length polymorphism (RFLP) analyses of the IGS-1 region with the AluI restriction enzyme were used to identify the isolates, because conspicuous, epigeous basidiomes could not be found in or near the fen. During these analyses, two previously unpublished fragment patterns for A. sinapina were found, consisting of 399–240–135 base pairs (bp) and 399–240–183–135 bp. Interspecific somatic incompatibility and interfertility tests confirmed the identifications obtained from the RFLP analyses. This is the first report of an annulate species of Armillaria being actively involved in the colonization and decomposition of herbaceous plant material in an ecosystem having only an organic soil horizon. Surveys for species of Armillaria should be expanded to include peatlands and herbaceous plant materials, because they may serve as vast potential biomass and genetic diversity reservoirs for this genus.Key words: peatland, Carex rhizomes, Salix leaves, RFLP, interspecific somatic incompatibility, interfertility.

2019 ◽  
Vol 25 (1) ◽  
Author(s):  
NARENDRA SINGH ◽  
N. S. BHADAURIA ◽  
PRADYUMN SINGH

The Bio-efficacy of eleven plant extracts namely viz.Neem Kernel; Rhizome of Ginger; Leaves of Datura, Gajarghas, Harsingar, Oak and Latjeera; Bulb of Garlic and Onion; Flowers of Chrysenthemum and Fruits of Chilli in the concentration of 5 percent and imidacloprid @ 40 g ai/ha was tested against mustard aphid, Lipaphiserysimi and their effect on D. rapae and Coccinellid beetle were tested in the Department of Entomology, College of Agriculture, Gwalior (M.P.). All the tested plant materials and imidacloprid @ 40 g ai/ha were effective significanty in reducing the aphid population over control.The aphid population in treated plots ranged from 7.2 to 40.0 as against 85.4 aphid/twig in untreated control. Among the plant material, three sprays of Neem Kernel were found most effective followed by three sprays of chilli fruits.All the plant extracts were found significantly safer to D. rapae and coccinellid bettle in comparision to insecticide (imidacloprid).


Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1406 ◽  
Author(s):  
Muhammad Modassar A. N. Ranjha ◽  
Shafeeqa Irfan ◽  
José M. Lorenzo ◽  
Bakhtawar Shafique ◽  
Rabia Kanwal ◽  
...  

Traditional extraction techniques have lost their optimum performance because of rising consumer demand and novel technologies. In this regard, several techniques were developed by humans for the extraction of plant materials from various indigenous sources, which are no longer in use. Many of the techniques are not efficient enough to extract maximum plant material. By this time, evolution in extraction has led to development of various techniques including microfiltration, pulsed electric fields, high pressure, microwave assistance, enzyme assistance, supercritical fluid, subcritical fluid and ultrasonication. These innovations in food processing/extraction are known as “Green Food Processing”. These technologies were basically developed by focusing on three universal parameters: simplicity, energy efficiency and economy. These green technologies are practical in a number of different food sectors, mostly for preservation, inhibition of microorganisms, inactivation of enzymes and extraction of plant material. Like the others, ultrasonication could also be used for the said purposes. The primary objective of this review is to confine the potential use of ultrasonication for extraction of oils, pectin and phytochemicals by reviewing the literature systematically.


Botany ◽  
2012 ◽  
Vol 90 (6) ◽  
pp. 457-464 ◽  
Author(s):  
Therese A. Thompson ◽  
R. Greg Thorn ◽  
Kevin T. Smith

Fungi in the Agaricomycetes (Basidiomycota) are the primary decomposers in temperate forests of dead wood on and in the forest soil. Through the use of isolation techniques selective for saprotrophic Agaricomycetes, a variety of wood decay fungi were isolated from a northern hardwood stand in the Bartlett Experimental Forest, New Hampshire, USA. In particular, Hypholoma lateritium (Schaeff.: Fr.) P. Kumm. was isolated from basidiocarps, decaying Acer rubrum L. logs, the Oe organic soil horizon, and the E and BC mineral soil horizons. Identification was confirmed by sequence analysis of the internal transcribed spacer region of nuclear ribosomal DNA. All isolates had identical sequences in this region to previously published sequences for the species; some were monokaryotic and simple-septate and others were dikaryotic, with clamp connections. Isolates were further characterized by banding patterns (DNA fingerprints) produced with PCR primers based in simple repetitive sequences and the minisatellite M13. Nine dikaryotic isolates from basidiocarps and from soil horizons Oe, E, and BC had identical fingerprint patterns with all primers tested. The confirmed presence of H. lateritium suggests that this fungus could form a mycelial translocation network that bridges mineral and organic soil horizons and decaying logs.


Ecology ◽  
2014 ◽  
Author(s):  
Danny J. Gustafson ◽  
Alexis Gibson

Ecological restoration is most commonly described as the process of aiding in the recovery of a damaged or destroyed system. In many cases, restoration may not be possible when self-sustaining populations, functions, and trajectories cannot be maintained due to the type of disturbance sustained by a site; in these cases, revegetation or remediation are more achievable goals. The definition of ecological restoration has been expanded to incorporate scientific inquiry into the process of the recovery of a natural range of ecosystem composition, structure, and dynamics. Ecological restoration research spans different levels of organization from genes to ecosystems. Genetic considerations are fundamental to the success of ecological restoration, and considerations of this issue will impact choices from seed source selection to genetic control of ecosystem services. A major decision for restorationists is the use of local versus nonlocal plant material, as well as the mixing of source populations; ideally, these choices can be based on sound population genetic, ecological, and evolutionary theory research. Ultimately, selection of plant material to be used in ecological restoration is driven by the specific project goals, availability and quality of plant materials, site conditions, and scale of the project. Beyond the local versus nonlocal selection issue, genetic issues related to small population dynamics, gene flow in the modern landscape, and gene expression affecting community structure and ecosystem functions can affect the success of ecological restoration activities. This article focuses primarily on plants; however, issues related to genetics of small populations (inbreeding and outbreeding depression, founder effects, and fitness consequences of reduced genetic variation) are important considerations for animal species too. The readings contained within this bibliography include: Ecotypic Variation, Seed Provenance for Restoration, Seed Transfer Zones for Restoration, Seed Provenance for Revegetation, Life History Traits, Moving beyond Neutral Markers, Inbreeding Depression, Outbreeding Depression, Founder Effects, Fitness Consequences of Reduced Genetic Variation, Community and Landscape Genetics, Testing Genotypic Effects on Community and Ecosystem Processes, Evaluating Success, and Genetic Composition and Diversity in Restored Populations.


1980 ◽  
Vol 63 (4) ◽  
pp. 770-778 ◽  
Author(s):  
Darrell W Nelson ◽  
Lee E Sommers

Abstract Progress of the Dumas and Kjeldahl procedures over the past century is reviewed. Many recent papers claim that various modifications of the standard Kjeldahl method enhance accuracy, precision, or speed, and reduce cost of analysis. Furthermore, several authors advocate use of Pyrex tubes heated in an aluminum block for digestion instead of traditional Kjeldahl flasks. A review of current semimicro-Kjeldahl methodology suggests that proceeding under the following conditions gives satisfactory results: sample size, 50–200 mg for plant material, 100–500 mg for soil; digestion acid, concentrated H2SO4; sample size ratio (mL/g) of 16:1 for soils and 22:1 for plant materials; digestion salt, minimum 0.33 g K2SO4 added/mL H2SO4; catalysts, add CuSO4 5H2O at rate of 10% (w/w) of K2SO4 added, HgO at 5% (w/w) of K2SO4, or add Se to K2SO4–CuSO4 5H2O mixtures at rate of 1% (w/w) K2SO4; digestion time, 1 and 3 h past clearing for plant materials and soils, respectively; pretreatments, use salicylic acid or reduced iron to recover nitrate from sample. Use of Pyrex tubes heated in an aluminum block appears suitable for digestions. Our results indicate that the 2 commercial tube digestion systems tested produce satisfactory recovery of total N from soils and plant materials. Ammonium in Kjeldahl digests may be quantitatively determined by distillationtitration, ammonia electrode, or colorimetric techniques.


Safety ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 56 ◽  
Author(s):  
Nikolay Baranovskiy ◽  
Alena Demikhova

The last few decades have been characterized by an increase in the frequency and burned area of forest fires in many countries of the world. Needles, foliage, branches, and herbaceous plants are involved in burning during forest fires. Most forest fires are surface ones. The purpose of this study was to develop a mathematical model of heat transfer in an element of combustible plant material, namely, in the stem of a herbaceous plant, when exposed to radiation from a surface forest fire. Mathematically, the process of heat transfer in an element of combustible plant material was described by a system of non-stationary partial differential equations with corresponding initial and boundary conditions. The finite difference method was used to solve this system of equations in combination with a locally one-dimensional method for solving multidimensional tasks of mathematical physics. Temperature distributions were obtained as a result of modeling in a structurally inhomogeneous stem of a herbaceous plant for various scenarios of the impact of a forest fire. The results can be used to develop new systems for forest fire forecasting and their environmental impact prediction.


1965 ◽  
Vol 43 (8) ◽  
pp. 1277-1280 ◽  
Author(s):  
Hirokazu Morita

Hot alkaline hydrolysis of an organic soil followed by multiple unidimensional paper chromatographic analyses have afforded p-coumaric, ferulic, sinapic, and 3,5-dihydroxybenzoic acids. It is suggested that part of the phenolic acids may be derived from non-lignin plant materials incorporated into the humus.


Radiocarbon ◽  
2014 ◽  
Vol 56 (02) ◽  
pp. 679-690
Author(s):  
V Caracuta ◽  
G Fiorentino ◽  
M Turchiano ◽  
G Volpe

Charred plant materials found in archaeological contexts are usually considered the most reliable remains for radiocarbon dating. Usually, seeds and fruits are preferred to wood fragments because their short lifecycle reduces the range of uncertainty of the14C measurement. A selection of short-lived samples, mainly from barley and wheat, from the Late Roman site of Faragola (SE Italy) were14C dated; however, the14C dates obtained were not always consistent with the chronology provided by other archaeological evidence. A careful analysis of all the macrobotanical remains found in each of the dated contexts provides insight into the origin of the plant material, helping to distinguish betweenin situand non-in situmaterial. The14C dates are reconsidered in the context of findings and the kind of material selected for dating. Using the archaeological context, a Bayesian model was employed to reduce the range of the calibrated date and thereby refine the chronology of the site on an absolute basis.


Tribologia ◽  
2016 ◽  
Vol 270 (6) ◽  
pp. 191-204
Author(s):  
Artur WÓJCIK ◽  
Jarosław FRĄCZEK

The results of studies on external friction concerning granular plant materials that include, among others, grains, seeds of various plants, and powdered plant material, have been applied to the design of machines and devices for transport, sorting, cleaning, and blending. A separate and equally important matter is the storage of these materials in silos. The article presents the influence the surface topography of selected granular materials has upon the force of external friction. The research was performed with a specially designed adapter attached to the testing machine MTS, which is used to determine the external friction force of granular materials rubbing against various surfaces in a function of displacement. To define the topography of the external surface, a 3D scanner and specialized software were used. The authors have attempted to develop a model of friction taking into account the topography of the surface.


Genome ◽  
1992 ◽  
Vol 35 (3) ◽  
pp. 516-527 ◽  
Author(s):  
Shahryar F. Kianian ◽  
Carlos F. Quiros

Sequences homologous to rRNA, napin, cruciferin, self-incompatibility, isocitrate lyase, and malate synthase were mapped to the Brassica oleracea genome. Four segregating populations were used to disclose possible distortions in segregation and linkage ratios while maximizing detectable polymorphism. rRNA mapped to three unlinked loci, which reside on different chromosomes. Certain restriction fragment length polymorphism variations detected by the rDNA probe reflect changes in the number of intergenic spacer subrepeats, the size of which was estimated to be about 300 base pairs. All five napin, three self-incompatibility, and two isocitrate lyase loci mapped in linkage clusters, while those of cruciferin and malate synthase (two loci each) were independent.Key words: Brassica oleracea, RFLP analysis, linkage analysis, multigene family.


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