A comparison of ITS and LSU nrDNA phylogenies of Fulgensia (Teloschistaceae, Lecanorales), a genus of lichenised ascomycetes

2000 ◽  
Vol 78 (12) ◽  
pp. 1580-1589 ◽  
Author(s):  
Thomas Kasalicky ◽  
Heidi Döring ◽  
Gerhard Rambold ◽  
Mats Wedin
Keyword(s):  
Maximum Parsimony ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Morphological Characters ◽  
Nuclear Ribosomal Dna ◽  
Internal Transcribed Spacer Region ◽  
Data Set ◽  
Spliceosomal Introns ◽  
Nuclear Its ◽  
Combined Data

The phylogeny of the lichen genus Fulgensia Massal. & De Not. (Teloschistaceae, Lecanorales) is analysed using maximum parsimony and neighbor joining analyses of nuclear ITS and partial large subunit nuclear ribosomal DNA (nrDNA) sequences. Three matrices were analysed with maximum parsimony; an internal transcribed spacer nrDNA matrix, a large subunit nrDNA matrix, and a combined data set. The internal transcribed spacer region contributes 70% of the informative sites to the combined data set. The topology of the trees resulting from the analysis of the internal transcribed spacer region is identical to the tree topology resulting from the combined analysis, but it shows less resolution at basal parts of the tree. Two sites for putative spliceosomal introns in the large subunit nrDNA, at position 808 and 914 (relative to Saccharomyces cerevisiae) are reported. Fulgensia, as currently understood, is polyphyletic and some species have to be excluded. The molecular analyses identified groups of species within the genus that are also supported by anatomical and morphological characters. The results of the analyses are compared with existing classification concepts based on morphological and anatomical data.Key words: LSU, ITS, nrDNA, introns, Fulgensia, phylogeny.

Phylogenetic analyses of Nematoctonus and Hohenbuehelia (Pleurotaceae)

2007 ◽  
Vol 85 (8) ◽  
pp. 762-773 ◽  
Author(s):  
Alexandra T.E. Koziak ◽  
Kei Chin Cheng ◽  
R. Greg Thorn
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Nuclear Ribosomal Dna ◽  
Monophyletic Clade ◽  
Parasitoid Species ◽  
Internal Transcribed Spacer Region ◽  
Species Limits ◽  
Additional Gene

Hohenbuehelia (Agaricales, Pleurotaceae) and Nematoctonus (Hyphomycetes) are the names for the sexual and asexual stages of a genus of nematode-destroying fungi (Basidiomycota). We obtained partial sequences of nuclear ribosomal DNA, including the internal transcribed spacer region and the 5′ end of the large subunit, of 37 isolates of Hohenbuehelia and Nematoctonus representing 13 of the 16 described species in Nematoctonus. Phylogenetic analyses support Hohenbuehelia–Nematoctonus as a monophyletic clade of the Pleurotaceae, within which the species were placed in five main subclades. Exclusively predatory species ( Nematoctonus brevisporus Thorn & G.L. Barron, Nematoctonus campylosporus Drechsler, Nematoctonus robustus F.R. Jones, and Nematoctonus sp. UAMH 5317) appear to be basal. In these species, adhesive knobs to capture prey are produced on their hyphae but not on their conidia. A single mycelial individual may feed on many nematodes. From these have arisen both exclusively parasitoid species ( Nematoctonus cylindrosporus Thorn & G.L. Barron, Nematoctonus leiosporus Drechsler, Nematoctonus leptosporus Drechsler, Nematoctonus pachysporus Drechsler, Nematoctonus tylosporus Drechsler), and species that we call intermediate predators ( Nematoctonus angustatus Thorn & G.L. Barron, Nematoctonus concurrens Drechsler, Nematoctonus geogenius Thorn & GL. Barron, Nematoctonus hamatus Thorn & G.L. Barron, and Nematoctonus subreniformis Thorn & G.L. Barron). Exclusively parasitoid species have conidia that germinate to form sticky knobs that attach to passing nematodes but lack adhesive knobs on the hyphae. Each mycelial individual feeds on only one nematode. Intermediate predators have adhesive knobs both on hyphae and on germinated conidia and can act in both predatory and parasitoid modes. Most morphospecies are resolved as monophyletic, but sequences of additional gene regions are required to clarify species limits within the N. angustatus – N. geogenius group.

scholarly journals Molecular systematics of Keratinophyton: the inclusion of species formerly referred to Chrysosporium and description of four new species

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Roman Labuda ◽  
Andreas Bernreiter ◽  
Doris Hochenauer ◽  
Alena Kubátová ◽  
Hazal Kandemir ◽  
...  
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Its Region ◽  
Soil Samples ◽  
Lsu Rdna ◽  
Internal Transcribed Spacer Region ◽  
Its Phylogeny ◽  
Combined Data ◽  
Terminal Cluster

AbstractFour new Keratinophyton species (Ascomycota, Pezizomycotina, Onygenales), K. gollerae, K. lemmensii, K. straussii, and K. wagneri, isolated from soil samples originating from Europe (Austria, Italy, and Slovakia) are described and illustrated. The new taxa are well supported by phylogenetic analysis of the internal transcribed spacer region (ITS) region, the combined data analysis of ITS and the nuclear large subunit (LSU) rDNA, and their phenotype. Based on ITS phylogeny, within the Keratinophyton clade, K. lemmensii is clustered with K. durum, K. hubeiense, K. submersum, and K. siglerae, while K. gollerae, K. straussii and K. wagneri are resolved in a separate terminal cluster. All four new species can be well distinguished from other species in the genus based on phenotype characteristics alone. Ten new combinations are proposed for Chrysosporium species which are resolved in the monophyletic Keratinophyton clade. A new key to the recognized species is provided herein.

Molecular phylogeny of two coelomycetous fungal genera with stellate conidia,ProsthemiumandAsterosporium, on Fagales trees

Botany ◽  
2010 ◽  
Vol 88 (12) ◽  
pp. 1057-1071 ◽  
Author(s):  
Kazuaki Tanaka ◽  
Vadim A. Mel’nik ◽  
Maasa Kamiyama ◽  
Kazuyuki Hirayama ◽  
Takashi Shirouzu
Keyword(s):  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Diagnostic Value ◽  
Small Subunit ◽  
Nuclear Ribosomal Dna ◽  
Internal Transcribed Spacer Region ◽  
Phylogenetic Relatedness ◽  
Close Relationship ◽  
Phylogenetic Affinities ◽  
A New Species

Prosthemium (teleomorph Pleomassaria ) and Asterosporium (teleomorph unknown) are coelomycetous genera with stellate conidia on Fagales trees. Their morphological resemblance suggests their close relationship, but phylogenetic relatedness remains unknown. They have been distinguished on the basis of either conidiomatal morphology (pycnidia in Prosthemium and acervuli in Asterosporium) or their differing conidial septation (euseptate in Prosthemium and distoseptate in Asterosporium). To reveal their phylogenetic affinities and clarify reliable distinguishing phenotypical characters, five species of Prosthemium and two species of Asterosporium were investigated using sequences of the small subunit, large subunit, and internal transcribed spacer region of nuclear ribosomal DNA and β-tubulin gene from 43 isolates of these species. The analyses revealed the following: (i) Asterosporium typified by Asterosporium asterospermum on Fagus is a member of the Sordariomycetes and is distinct from Prosthemium belonging to the Dothideomycetes; (ii) Asterosporium betulinum on Betula species should be excluded from Asterosporium and transferred to Prosthemium as Prosthemium neobetulinum nom. nov.; (iii) conidial septation does not seem to have a diagnostic value, whereas conidiomatal morphology is useful in distinguishing both genera; (iv) the number and length of conidial arms are useful criteria in distinguishing Prosthemium species. A new species, Prosthemium intermedium sp. nov., found on both Betula ermanii and Betula maximowicziana , is described and illustrated.

scholarly journals Four new Keratinophyton species (Onygenaceae) from Europe

2021 ◽  
Author(s):  
Roman Labuda ◽  
Andreas Bernreiter ◽  
Doris Hochenauer ◽  
Alena Kubátová ◽  
Hazal Kandemir ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Its Region ◽  
Soil Samples ◽  
Lsu Rdna ◽  
Internal Transcribed Spacer Region ◽  
Its Phylogeny ◽  
Asexual Morphs ◽  
Combined Data ◽  
Terminal Cluster

Abstract Four new Keratinophyton species (Ascomycota, Pezizomycotina, Onygenales), K. gollerae, K. lemmensii, K. straussii and K. wagneri, isolated from soil samples originating from Europe (Austria, Italy and Slovakia) are described and illustrated. The new taxa are well supported by phylogenetic analysis of the internal transcribed spacer region (ITS) region, the combined data analysis of ITS and the nuclear large subunit (LSU) rDNA, and their phenotype. Based on ITS phylogeny, within the Keratinophyton clade, K. lemmensii is clustered with K. durum, K. hubeiense, K. submersum and K. siglerae, while K. gollerae, K. straussii and K. wagneri are resolved in a separate terminal cluster. All four new species can be well distinguished from other asexual morphs in the genus Keratinophyton based on phenotypical characteristics alone. Ten new combinations are proposed for Chrysosporium asexual morphs which are resolved in the monophyletic Keratinophyton clade.

Mitochondrial and nuclear ribosomal DNA data do not support the separation of the Antarctic lichens Umbilicaria kappenii and Umbilicaria antarctica as distinct species

2004 ◽  
Vol 36 (3-4) ◽  
pp. 227-234 ◽  
Author(s):  
Sieglinde OTT ◽  
Marcus BRINKMANN ◽  
Nora WIRTZ ◽  
H. Thorsten LUMBSCH
Keyword(s):  
Distinct Species ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Lsu Rdna ◽  
Data Set ◽  
Rdna Sequences ◽  
Group A ◽  
Nuclear Its ◽  
The Antarctic ◽  
Combined Data

The Antarctic endemics Umbilicariakappenii and U. antarctica are morphologically close, but mainly distinguished by their reproductive strategies. Umbilicaria antarctica propagates by means of thalloconidia. Umbilicaria kappenii lacks thalloconidia, but exhibits a variety of asexual propagules: soredia, adventive lobes and thallyles. We have now employed molecular data from three gene regions to examine the phylogenetic relationships of these two morphotypes. The phylogeny of ten samples and four outgroup taxa (Umbilicaria decussata, U. krascheninnikovii, U. nylanderiana, U. umbilicarioides) was reconstructed using Bayesian and maximum parsimony analyses of a combined data set of nuclear ITS, nuclear LSU rDNA and mitochondrial LSU rDNA sequences. Forty two new partial sequences of 14 specimens were generated. Our results indicate that all samples morphologically referred to U. antarctica and U. kappenii form a monophyletic group. A topology separating the two morphotypes as phylogenetic species is significantly rejected with the data set. It is proposed to place U. kappenii into synonymy with U. antarctica.

A new species of Chlorociboria (Helotiales, Ascomycota) on herbaceous stems from China

Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 111
Author(s):  
HUAN-DI ZHENG ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Central China ◽  
Nuclear Ribosomal Dna ◽  
Light Blue ◽  
Ectal Excipulum ◽  
A New Species

A new species, namely Chlorociboria herbicola, is discovered on herbaceous stems in central China. Morphologically, the new fungus is distinctive by the combination of light blue-green apothecia, rectangular cells in ectal excipulum, and elongate-ellipsoidal ascospores with rounded ends. Phylogenetic analyses of the internal transcribed spacer and large subunit of nuclear ribosomal DNA sequences confirm its ascription in Chlorociboria and distinction from the known species of the genus.

First report of Hericium cirrhatum from Pakistan

Mycotaxon ◽  
2020 ◽  
Vol 135 (4) ◽  
pp. 845-852
Author(s):  
Junaid Khan ◽  
Hassan Sher ◽  
Shah Hussain ◽  
Abdul Nasir Khalid
Keyword(s):  
Dna Sequence ◽  
Internal Transcribed Spacer ◽  
Morphological Characters ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
First Report ◽  
First Time

Hericium cirrhatum, a widespread but locally rare tooth fungus is reported for the first time from Pakistan. This species is characterized by white to cream semicircular basidiomata (usually arranged in tiers) with a granular to slightly spiny sterile upper surface, a fertile under surface with 10–15 mm long pointed spines, and ellipsoid to subglobose basidiospores. Morphological characters and DNA sequence analyses inferred from the nuclear ribosomal internal transcribed spacer region (nrITS) confirm the identity of the taxon.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 409 (4) ◽  
pp. 189-201 ◽  
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

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