Hyaluronidase and chondroitinase activity of Pasteurella multocida serotype B:2 involved in hemorrhagic septicaemia

1994 ◽  
Vol 134 (3) ◽  
pp. 67-68 ◽  
Author(s):  
R. Rimler ◽  
K. Rhoades
Keyword(s):  
Pasteurella Multocida ◽  
Serotype B

Virulence Gene Pattern of Pasteurella multocida Isolates of Buffalo in Association to Capsule Biosynthesis Genes

2020 ◽  
Author(s):  
N. Sujatha ◽  
K. Lakshmi Kavitha ◽  
K.V. Subramanyam ◽  
T. Srinivasa Rao ◽  
R.N. Ramani Pushpa
Keyword(s):  
Virulence Factors ◽  
Pasteurella Multocida ◽  
Virulence Genes ◽  
Systemic Disease ◽  
Virulence Gene ◽  
Disease Status ◽  
Host Cells ◽  
Wide Range ◽  
Serotype B ◽  
Apparently Healthy

Background: Pasteurella multocida is the causative agent of many economically important diseases in a wide range of hosts. The mechanisms by which these bacteria can invade the mucosa, evade innate immunity and cause systemic disease are slowly being elucidated. Many key virulence factors are yet to be identified, including those required for initial attachment and invasion of host cells and for persistence in a relatively nutrient poor and hostile environment. This has led to intensive research to understand host adaptation mechanisms and virulence factors in order to develop effective vaccines. Methods: The present study was carried out to know the distribution of virulence genes viz., haemoglobin binding proteins (hgbA and hgbB), outer membrane protein (ompH), fimbrial antigen (ptfA), filamentous haemagglutinin (pfhA) and transferrin binding protein (tbpA) by PCR in P. multocida CapA isolates from apparently healthy or carrier animals and CapB isolates from field Haemorrhagic septicemia (HS) cases to monitor the epidemiological associations of virulence genes in Cap A and Cap B isolates.Result: The study revealed that all the six virulence associated genes were present in Cap B isolates. None of the Cap A isolates harboured tbpA and pfhA genes. These two genes were closely related to serotype B causing Haemorrhagic septicemia and were epidemiologically associated with disease status.

scholarly journals First detection of Pasteurella multocida type B:2 in Hungary associated with systemic pasteurellosis in backyard pigs

2015 ◽  
Vol 63 (2) ◽  
pp. 141-156 ◽  
Author(s):  
Barbara Ujvári ◽  
Levente Szeredi ◽  
László Pertl ◽  
Gergely Tóth ◽  
Károly Erdélyi ◽  
...  
Keyword(s):  
Small Area ◽  
Pasteurella Multocida ◽  
Sequence Type ◽  
Type B ◽  
Chain Reaction ◽  
Haemorrhagic Septicaemia ◽  
Gene Typing ◽  
Polymerase Chain ◽  
Serotype B ◽  
Backyard Pigs

This is the first report of Pasteurella multocida type B in Hungarian pigs. This disease was observed in backyard-raised pigs in three households within a small area. Neither the source of the infection nor the epidemiological connection between any of the premises could be determined. The most consistent lesion was dark red discolouration of the skin of the ventral neck and brisket, with accompanying oedema and haemorrhages. The morbidity was low and lethality relatively high, with three dead (50%) and two euthanised (33%) out of six affected animals. A total of three isolates of P. multocida (P55, P56 and P57) were cultured from these cases and examined in detail. These were identified as P. multocida ssp. multocida biovar 3. All were toxA negative and belonged to serotype B:2. Multilocus sequence typing was used to assign these to a new sequence type (ST61) that is closely related to other haemorrhagic septicaemia causing strains of P. multocida regardless of the host. M13 polymerase chain reaction and virulence-associated gene typing also show that type B strains form a highly homogeneous, distinct phylogenic group within P. multocida.

scholarly journals Re-emergence of bovine haemorrhagic septicaemia in Hungary

2017 ◽  
Vol 65 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Tibor Magyar ◽  
Barbara Ujvári ◽  
Levente Szeredi ◽  
Norbert Virsinger ◽  
Ervin Albert ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Animal Health ◽  
Small Farm ◽  
Beef Calves ◽  
Haemorrhagic Septicaemia ◽  
Lymph Node Enlargement ◽  
Laboratory Examinations ◽  
Serotype B ◽  
Loss Of Appetite ◽  
World Organisation

This paper reports an outbreak of haemorrhagic septicaemia caused by Pasteurella multocida B:2 in beef calves, a disease that has not been described in the Hungarian literature since 1943, and has not been reported to the World Organisation For Animal Health (OIE) since 1970. Acute haemorrhagic septicaemia was confirmed in beef calves on one small farm, and was suspected on two further nearby holdings with concomitant unexplained losses. The source of the infection could not be determined. Apart from a short duration of depression and loss of appetite, the affected calves developed characteristic distal limb oedema. Gross findings in two calves submitted for laboratory examinations included subcutaneous oedema and haemorrhages on serous membranes, and in one case severe pharyngeal lymph node enlargement was observed. Histological examinations revealed lesions characteristic of septicaemia. Moderate to large amounts of Pasteurella antigens were detected in all organs tested by immunohistochemistry. Two isolates of P. multocida (Pm240, Pm241) were cultured from these cases and examined in detail. These were identified as P. multocida ssp. multocida biovar 3. Both were toxA negative and belonged to serotype B:2. Multilocus sequence typing was used to assign these to a new sequence type (ST64) that is closely related to other haemorrhagic septicaemia causing strains of P. multocida regardless of the host.

scholarly journals Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B

2011 ◽  
Vol 5 (18) ◽  
pp. 2596-2599 ◽  
Author(s):  
Hussaini Jamal ◽  
M H M Nazmul ◽  
A Abdullah Mahmood ◽  
Ismail Salmah
Keyword(s):  
Pasteurella Multocida ◽  
Laboratory Animals ◽  
Recombinant Clone ◽  
Serotype B

scholarly journals Molecular typing of Indian isolates of Pasteurella multocida serotype B:2 by ERIC- PCR

2016 ◽  
Vol 3 (9) ◽  
pp. 232-237
Author(s):  
M.K. Saxena ◽  
◽  
A. Kumar ◽  
V.P. Singh ◽  
P. Chaudhari ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Pasteurella Multocida ◽  
Indian Isolates ◽  
Serotype B

scholarly journals Septicémie hémorragique (Pasteurella multocida sérotype E:2) expérimentale chez des veaux : étude clinique, pathologique et microbiologique

2005 ◽  
Vol 58 (3) ◽  
pp. 133 ◽  
Author(s):  
M. O. Odugbo ◽  
U. A. Turaki ◽  
A. E. Itodo ◽  
A. E.J. Okwori ◽  
R. A. Yakubu
Keyword(s):  
Pasteurella Multocida ◽  
Étude Clinique ◽  
Serotype B ◽  
Signes Cliniques

Une septicémie hémorragique (SH) a été induite expérimentalement chez des veaux de sept mois (n = 2) par inoculation intratrachéale de 1010 unités formant colonies d’une culture de Pasteurella multocida sérotype E:2 de 6 h en phase de croissance exponentielle pour étudier l’aspect clinicopathologique de l’infection et faire l’analyse microbiologique. La période d’incubation a duré quatre heures après l’infection. La série de signes cliniques généraux, classés par ordre de leur manifestation, ont été les suivants : fièvre, anorexie, dyspnée, gonflement de la région gorge-membres avants, tympanisme, jetage, salivation abondante, léthargie, décubitus et mort. A la nécropsie, les lésions prédominantes observées ont été : congestion pulmonaire avec symphyse pleurale des lobes craniaux, pleurésie, gonflement oedémateux du pharynx et du fanon avec exsudation d’un liquide jaunâtre semblable au sérum, et hémorragies dermiques et ecchymotiques. L’analyse histologique a révélé que les lésions pulmonaires étaient typiques de celles de la broncho-pneumonie fibrineuse avec épaississement des parois alvéolaires, congestion, oedèmes et réponse cellulaire des poumons. A la nécropsie, P. multocida sérotype E:2 a été à nouveau isolé bactériologiquement des poumons, des noeuds lymphatiques, du foie, des reins, de la rate, du fluide oedémateux et du sang cardiaque. Cet agent pathogène n’a pas été détecté dans le sang veineux jusqu’à quelques heures avant la mort. Les aspects cliniques et pathologiques observés sur les animaux ont montré des ressemblances frappantes avec P. multocida sérotype B:2 HS. Ces données devraient permettre aux vétérinaires de reconnaître les cas suspects de SH sur le terrain.

scholarly journals Cloning and sequence analysis of hsf, an outer membrane protein gene of Pasteurella multocida serotype B:2

2014 ◽  
Vol 7 (12) ◽  
pp. 1090-1093 ◽  
Author(s):  
A. Priyadarshini ◽  
S. Kumar ◽  
S. K. Gupta ◽  
K. N. Viswas ◽  
R. K. Agarwal ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Pasteurella Multocida ◽  
Protein Gene ◽  
Serotype B ◽  
Membrane Protein Gene
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