Equine whole saliva: a sample collection system and biochemical analysis

1984 ◽  
Vol 115 (17) ◽  
pp. 437-438 ◽  
Author(s):  
P. Eckersall
Keyword(s):  
Biochemical Analysis ◽  
Sample Collection ◽  
Collection System ◽  
Whole Saliva

The integrated blood-collection system as a vehicle into complete clinical laboratory automation

1991 ◽  
Vol 37 (9) ◽  
pp. 1548-1556 ◽  
Author(s):  
R L Columbus ◽  
H J Palmer
Keyword(s):  
Clinical Laboratory ◽  
Integrated System ◽  
Diagnostic Instrument ◽  
Laboratory Evaluation ◽  
Blood Collection ◽  
Sample Collection ◽  
Collection System ◽  
Patient Identification ◽  
Plasma Extraction ◽  
Derivatives Of

Abstract A rationale is offered and methodology illustrated for integrating the fundamental steps involved in the collection and processing of blood for laboratory evaluation. The approach taken in the development of these concepts and components greatly extends the possibilities of laboratory systems integration without upsetting established modalities. A prototype design of the integrated blood-collection system integrates blood collection, cellular separations, sample transfer to stable storage without chemical mediators, and sample presentation for chemical analysis (e.g., precision metering) while preserving patient identification. A sophisticated, multi-chambered blood-collection container is the site of all blood sample processing and transfer steps. This device is supported by a compact, robotic centrifuge of unique design and a transfer mechanism to facilitate sample delivery for analysis within a diagnostic instrument. The confluence of these individual components into a single integrated system provides the means to completely automate the processing of blood samples, after sample collection, eliminating all manual transfer steps and any external exposure of blood interfaces outside the diagnostic instrument. Configurational derivatives of the Integrated Blood-Collection System offer choice of skin or venipuncture procedure, rapid plasma extraction for micro- or macro-collected volumes, and sample delivery by either aspiration or direct metering of discrete 10-microL samples from the collection container. The skin-puncture configuration provides the opportunity within a single device to collect and process up to 500 microL of sample by capillarity from a skin prick.

A 24-Hour Air Sample Collection System

2010 ◽  
Author(s):  
K A Janni ◽  
N Akdeniz ◽  
D R Schmidt ◽  
L D Jacobson ◽  
B P Hetchler
Keyword(s):  
Sample Collection ◽  
Collection System ◽  
Air Sample

scholarly journals Sistema de Coleta e Documentação de Microalgas

2020 ◽  
Author(s):  
Mateus Belli ◽  
Thiago Felski Pereira ◽  
Alex Luciano Roesler Rese
Keyword(s):  
Embedded System ◽  
Agricultural Development ◽  
Bivalve Mollusc ◽  
Sample Collection ◽  
Collection System ◽  
Santa Catarina ◽  
Great Loss ◽  
Sample Water ◽  
To Come ◽  
Harmful Microalgae

The multiplication of harmful microalgae in bivalve mollusc fishingregions is a problem that has repercussions for both fishermenand for the economy of the affected region, as these microalgaeproduce toxins that affect humans, ranging from gastrointestinalproblems to, in more extreme cases, cancer that can lead to thedeath of the consumer. Currently, in Santa Catarina, the water isanalyzed to identify the presence of toxins by the Integrated Companyof Agricultural Development of Santa Catarina (CIDASC). Thepresence of toxins in the water creates a temporary embargo onthe sale of mollusks. This embargo usually produces great loss inthe bivalve mollusc culture as far as all the production needs do bediscarded. This work creates a sample collection system that canbe performed by growers rather than experts for the acquisition ofthose samples. These work aims to create an easy-to-use systemenabling the mariculturist to sample water. The purposed processallows the acceleration of the sample collection and analysys. Themanual process needs an expert to come to the field, collect samples,document the samples and take to the lab to analyze. In thisstudy we created an embedded system for sample collection thatautomated the collect field data and link to a image collected bya portable microscope and when a wifi connection is detected itsends the sample to a data server.

Salivary Raman Spectroscopy: Standardization of Sampling Protocols and Stratification of Healthy and Oral Cancer Subjects

2020 ◽  
pp. 000370282097326
Author(s):  
Arti Hole ◽  
Gunjan Tyagi ◽  
Atul Deshmukh ◽  
Raviraj Deshpande ◽  
Vikram Gota ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Oral Cancer ◽  
Minimally Invasive ◽  
Cancer Diagnostics ◽  
Spectral Features ◽  
Sample Collection ◽  
Linear Discriminant ◽  
Sampling Protocols ◽  
Whole Saliva

Minimally invasive cancer detection using bio-fluids has been actively pursued due to practical limitations, though there are better suited noninvasive and online in vivo methods. Saliva is one such clinically informative bio-fluid that offers the advantages of easy and multiple sample collection. Despite its potential in cancer diagnostics, saliva analysis is challenging due to its heterogeneous composition. Recently, there has been an upsurge in saliva exploration using optical techniques. Forms of saliva such as precipitate and supernatant have been monitored, but this sampling method needs to be standardized due to the obvious loss of analytes in processing. In that context, present work details the comparison of four different saliva sampling methodologies, i.e., air-dried, lyophilized, pellet, and supernatant using Raman spectroscopy collected from 10 healthy samples. Composition-driven spectral features of all forms were compared and classified using principal component analysis and linear discriminant analysis. Analysis was carried out on all four groups in the first step. In the second step, groups of pellet and supernatant , and air-dried and lyophilized were analyzed. Findings suggest that pellet and supernatant exhibit discrete spectroscopic features and demonstrate high classification efficiency, which is indicative of their distinctive biochemical composition. On the other hand, air-dried and lyophilized forms showed overlapping spectral features and low classification, suggesting these forms retain majority spectroscopic features of whole saliva and are less prone to sampling losses. Thus, this study indicates air-dried and lyophilized forms may be more appropriate for saliva sampling using Raman spectroscopy providing the comprehensive information required for cancer diagnosis. Furthermore, the method was also tested for the classification of oral cancer and healthy subjects ( n = 27) which yielded 90% stratification. The findings of the study indicate the utility of minimally invasive salivary Raman-based diagnostics in oral cancers.

Carbon monoxide in blood: an improved microliter blood-sample collection system, with rapid analysis by gas chromatography.

1984 ◽  
Vol 30 (8) ◽  
pp. 1382-1386 ◽  
Author(s):  
H J Vreman ◽  
L K Kwong ◽  
D K Stevenson
Keyword(s):  
Gas Chromatography ◽  
Carbon Monoxide ◽  
Blood Sample ◽  
Premature Infants ◽  
Rapid Analysis ◽  
Analysis Time ◽  
Sensitive Assay ◽  
Sample Collection ◽  
Collection System ◽  
Significant Difference

Abstract We examined the sensitive assay for carboxyhemoglobin based on reaction with K3Fe(CN)6 and gas chromatography of the liberated CO. Our improvements included increased baseline stability, shorter analysis time, and simpler standardization. EDTA-containing Vacutainer Tubes (lavender-stoppered) increase the carboxyhemoglobin content of blood stored in them. The carboxyhemoglobin content of blood stored in capillary tubes containing solid heparin and saponin remained stable for two weeks. Using our improved procedures, we measured the carboxyhemoglobin content of blood from adults and neonates collected via venipuncture or heel or fingersticks. We observed no significant difference in carboxyhemoglobin content of blood obtained by venipuncture or heel stick for premature infants, 0.19 +/- 0.04 vs 0.18 +/- 0.03 mL of CO per 100 mL of blood, respectively (mean +/- SD). Nonsmoking adults (n = 19) had CO values (mean +/- SD) of 0.19 +/- 0.03 and 0.17 +/- 0.04 mL per 100 mL of blood, and smoking adults (n = 7) gave CO values of 0.96 +/- 0.49 and 0.91 +/- 0.49 mL/dL, for venipuncture and fingerstick, respectively.

Specific Sialochemical and Sialometric Changes and Cariogenic Risk in Patients with Primary Sjogren`s Syndrome

2017 ◽  
Vol 68 (9) ◽  
pp. 2135-2138
Author(s):  
Codrina Ancuta ◽  
Cristina Angela Ghiorghe ◽  
Rodica Chirieac ◽  
Alice Arina Ciocan Pendefunda ◽  
Cristina Iordache
Keyword(s):  
Biochemical Analysis ◽  
Salivary Flow ◽  
Flow Rates ◽  
Increased Risk ◽  
Whole Saliva ◽  
Tooth Surfaces ◽  
Potential Impact ◽  
Bacterial Plaque ◽  
Saliva Flow ◽  
Saliva Composition

The main aim of our study was to perform a biochemical analysis of the saliva and to assess potential impact on oral health, particularly cariogenic issues, in patients with salivary secretory dysfunction such as primary Sjogren�s syndrome (pSSj). Consecutive pSSj and healthy age-matched controls were prospectively assessed for (i) whole saliva flow rates (unstimulated, RFR, and stimulated, RFS), pH and composition; (ii) cariogenic scores - decayed, missing and filled tooth surfaces (DMFS index), Silnes and Loe bacterial plaque index (PI). Salivary flow rates were significantly lower in pSSj for both unstimulated and stimulated tests (p[0.05), with a drop in pH as compared to healthy controls (p[005). Abnormal inorganic and organic saliva composition was reported in all cases comprising higher sodium, lower potassium and chloride concentrations, higher amylase activity and lower total protein (p[0.05). Statistically significant higher cariogenic scores particularly DMFS, were demonstrated in all pSSj (p[0.05). In conclusion, patients with pSSj are at increased risk to develop cariogenic issues due to both quantitative and qualitative salivary changes.

Assessment of an Improved Stack Sample Collection System for 3H and 14C

2013 ◽  
Vol 105 ◽  
pp. S119-S124
Author(s):  
John Jelinski ◽  
Linnea Wahl ◽  
Thomas Donovan
Keyword(s):  
Sample Collection ◽  
Collection System
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