scholarly journals Composite Fasciola hepatica faecal egg sedimentation test for cattle

2019 ◽  
Vol 184 (19) ◽  
pp. 589-589 ◽  
Author(s):  
John Graham-Brown ◽  
Diana J L Williams ◽  
Philip Skuce ◽  
Ruth N Zadoks ◽  
Stuart Dawes ◽  
...  
Keyword(s):  
Fasciola Hepatica ◽  
Sampling Strategy ◽  
Statistical Modelling ◽  
Composite Sample ◽  
Sedimentation Method ◽  
Faecal Samples ◽  
Modelling Techniques ◽  
Sample Testing ◽  
Sample Method ◽  
And Control

Options for diagnosing Fasciola hepatica infection in groups of cattle are limited. Increasing the opportunities for herd-level diagnosis is important for disease monitoring, making informed treatment decisions and for flukicide efficacy testing. The sensitivity of a simple sedimentation method based on composite faecal samples for the detection of fluke eggs in cattle was assessed through a combination of experimental and statistical modelling techniques. Initially, a composite sample method previously developed for sheep was used to investigate the sensitivity of composite sample testing compared with individual counts on the same samples in cattle. Following this, an optimised, validated, qualitative (presence-absence) composite sample field test was developed for cattle. Results showed that fluke egg counts obtained from a composite sample are representative of those expected from individual counts. The optimal sampling strategy was determined to be 10 individual 10 g samples (100 g composite sample) from which a 10 g subsample is taken for sedimentation. This method yielded a diagnostic sensitivity of 0.69 (95 per cent CI 0.5 to 0.85). These results demonstrate the validity and usefulness of a composite faecal egg sedimentation method for use in the diagnosis and control of F. hepatica in groups of cattle, with the caveat that a negative test should be followed up with a second test due to limitations relating to test sensitivity.

scholarly journals Comparison of two coprological methods for the veterinary diagnosis of fasciolosis

2005 ◽  
Vol 57 (2) ◽  
pp. 181-185 ◽  
Author(s):  
F. Kleiman ◽  
S. Pietrokovsky ◽  
S. Gil ◽  
C. Wisnivesky-Colli
Keyword(s):  
Endemic Area ◽  
Diagnostic Method ◽  
Staining Method ◽  
Fasciola Hepatica ◽  
False Negative ◽  
Sample Processing ◽  
Hepatica Infection ◽  
Sedimentation Method ◽  
Faecal Samples ◽  
Reliable Diagnostic Method

The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method.

scholarly journals Gastrointestinal nematodes and Fasciola hepatica in Norwegian cattle herds: a questionnaire to investigate farmers’ perceptions and control strategies

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Tonje Opsal ◽  
Ingrid Toftaker ◽  
Ane Nødtvedt ◽  
Lucy Jane Robertson ◽  
Kristoffer Relling Tysnes ◽  
...  
Keyword(s):  
Fasciola Hepatica ◽  
Production Systems ◽  
Control Strategies ◽  
Management Strategies ◽  
Gastrointestinal Nematodes ◽  
Pasture Management ◽  
Concurrent Use ◽  
Faecal Samples ◽  
Cattle Herds ◽  
And Control

Abstract Background Pasture management influences the prevalence and impact of the pasture parasites (PP) in cattle herds, which cause production-limiting disease worldwide. Evaluating farmer management strategies is vital when considering sustainable PP control practices. The aim of this questionnaire-based study was to describe the pasture management and control strategies regarding PP in Norwegian beef cattle (BC) and dairy cattle (DC) production systems with a focus on gastrointestinal nematodes (GIN) and Fasciola hepatica. Results A total of 745 responses from BC (return rate 20.5%) and 1347 responses from DC farmers (30.7%) were included. The mean total pasture time for DC was 4.2 months for first-season grazers and 4.3 months for second-season grazers and cows, while the corresponding finding in BC was 5.4 months. Home pasture was used for most of the pasture period, particularly for first-season grazer dairy heifers (81%), which were also commonly grazed on the same pasture every year (79%). For most farmers it was necessary for grazing areas to be used for cattle for more than one season (77% of BC farmers and 89% of DC farmers). However, changing the pasture during the season was common in both DC (67%) and BC (70%) herds. The majority of DC farmers (60%) stated that they did not consider that they had a problem with PP. Of the remaining 40%, few respondents could specify whether their herds had a problem due to infection by GIN (11%) or liver flukes (12%). Treatment for GIN was performed by 52% of DC and 34% of BC farmers. Diagnostic faecal samples were collected upon suspicion of parasitic disease by 5% of DC and 16% of BC farmers. Veterinarians were stated as a central source of information about parasite management and treatment. Conclusions Potential risks for exposure to PP were identified, such as use of the same pasture every year for first-season grazers and frequent use of home pasture. The perception of problems related to PP appeared low. Regular anthelmintic treatment without concurrent use of diagnostic faecal samples seems to be common practice.

scholarly journals Prevalence of gastrointestinal parasites of cattle in Greece

2018 ◽  
Vol 53 (4) ◽  
pp. 304 ◽  
Author(s):  
A. DIAKOU (Α. ΔΙΑΚΟΥ) ◽  
E. PAPADOPOULOS (Η. ΠΑΠΑΔΟΠΟΥΛΟΣ)
Keyword(s):  
Prevention And Control ◽  
Fasciola Hepatica ◽  
Gastrointestinal Parasites ◽  
Parasitic Species ◽  
Faecal Samples ◽  
Eimeria Spp ◽  
Buxtonella Sulcata ◽  
And Control ◽  
Paramphistomum Cervi ◽  
Of Greece

In this study, 600 faecal samples, collected from cattle of various parts of Greece, were examined with two different parasitological methods (sedimentation and flotation). The prevalence of the infection with one or more parasitic species was 53.5%. The animals were found to be infected with the following parasitic species: Entamoeba bovis (0.5%), Eimeria spp. (11.83%), Buxtonella sulcata (7.5%), Dicrocoelium dendriticum (4.5%), Fasciola hepatica (0.16%), Paramphistomum cervi (4.66%), Moniezia benedeni (1.83%), Strongyloidea (41.66%), Neoascaris vitulorum (3.33%) and Trichuris spp. (0.83%). The results are compared to those from similar studies in Greece or abroad. Suggestions for the prevention and control of cattle parasites are discussed. 

scholarly journals Prevalence of diarrhoea, and associated risk factors, in children aged 0-5 years, at two hospitals in Umuahia, Abia, Nigeria

2017 ◽  
Vol 9 (1) ◽  
pp. 7-14
Author(s):  
Adanma Florence Nwaoha ◽  
Camelita Chima Ohaeri ◽  
Ebube Charles Amaechi
Keyword(s):  
Risk Factors ◽  
Health Workers ◽  
Age Groups ◽  
Medical Centers ◽  
Faecal Samples ◽  
Potential Risk Factors ◽  
Stool Samples ◽  
The Government ◽  
Associated Risk Factors ◽  
And Control

Diarrhoea is the second leading cause of infectious mor­bidity and mortality in children under five years of age. This study aimed at identifying the most common parasites and potential risk factors for diarrhoea among children 0-5 years attending Abia State Specialist hospital and Federal Medical Centre, Umuahia, in south east­ern Nigeria. We used 400 faecal samples from children with diarrhoea –and 200 without– in combination with hospital-based case control and a questionnaire Stool samples were processed with direct normal saline and formal-ether sedimentation method for parasitological stud­ies. More males than females were infected in nearly all age groups in both diarrhoeal and control groups (X2=23.04, df=1, P<0.05: X2=11.52, df=1, P<0.05 respectively). Amachara had more infections (X2=0.15, df=1, P< 0.05). January had the highest rate of infection (22.5%). Main clinical features were watery depositions over 3 times a day, diarrhoea lasting for days, fever, vomiting, and dehydration. Mothers learned about the problem through health workers, television and in medical centers. Risk correlated with mother’s education, occupation, latrine type, waste water disposal, hand washing, kitchen cleaning; sources and storage of water; and bottle milk (P< 0.05).Ignorance greatly con­tributed to the spread of parasitic disease in the area: the government should improve education and other strategies to alleviate the spread of the disease..

Use of Statistical Modelling Techniques for Simulation

1993 ◽  
Author(s):  
Ebrahim Mazharsolook ◽  
David C. Robinson ◽  
Jonathan D. Casey
Keyword(s):  
Quality Control ◽  
Statistical Modelling ◽  
Test Results ◽  
Final Test ◽  
Product Model ◽  
Design Changes ◽  
Discrete Manufacturing ◽  
Similar Product ◽  
Modelling Techniques

Abstract Statistical methods are explored for the use in modelling of discrete manufacturing. The developed methodologies based on Design of Experiments (DOE) and stepwise regression to obtain the product model are described. This model is then embedded within a software system which is used for simulation of design changes, process changes and disturbances. The software is used to predict final test results in respect of up-stream parameter changes. A case study is presented o show the implementation of this method of modelling in Quality Control of manufacture. This case study has successfully been implemented. The system is currently assisting the company in design of similar product. Feasibility of applying Artificial Intelligen (AI) techniques to Model-Based Quality Control (MBQC) is investigated. An outline of the future development of Hybrid MBQC is then presented.

Reserving for catastrophe reinsurance

1994 ◽  
Vol 121 (1) ◽  
pp. 135-160 ◽  
Author(s):  
D. H. Craighead
Keyword(s):  
Normal Development ◽  
Statistical Modelling ◽  
Development Pattern ◽  
Catastrophe Losses ◽  
Modelling Techniques ◽  
Oil Rig ◽  
Catastrophe Reinsurance ◽  
A Company

AbstractThe paper sets out the method required to be followed when estimating reserves for a Company or a Lloyd's Syndicate which has accepted reinsurance treaties that have given rise to catastrophe losses, sufficiently large to upset the normal development pattern and to affect the gross account quite differently from the net account. The losses may be caused by single factors such as aircraft crashes or oil rig disasters, or by the aggregation of claims resulting from a windstorm or an earthquake. The paper discusses two possible approaches to estimation of the gross losses; via exposure totals or via statistical modelling techniques.

Gastrointestinal parasites as a possible threat to an endangered autochthonous Portuguese sheep breed

2019 ◽  
Vol 94 ◽  
Author(s):  
Z.M. Ruano ◽  
A. Cortinhas ◽  
N. Carolino ◽  
J. Gomes ◽  
M. Costa ◽  
...  
Keyword(s):  
Fasciola Hepatica ◽  
Gastrointestinal Parasites ◽  
Sheep Breed ◽  
Parasitic Infections ◽  
Factors Affecting ◽  
Feed Management ◽  
Faecal Samples ◽  
High Prevalence ◽  
Protozoan Infections ◽  
Mcmaster Method

Abstract Helminth and protozoan infections are responsible for important diseases in grazing sheep, which can be especially threatening in an autochthonous breed at risk of extinction like the Churra Galega Mirandesa Portuguese sheep breed. The aim of the present study was to determine the diversity, prevalence and burden of gastrointestinal parasites in these sheep and to assess the effects of deworming practices, cohabiting animals on the farm and feed management. Coprological qualitative and quantitative analysis (flotation, natural sedimentation and McMaster method) were used to identify and quantify gastrointestinal parasites and a questionnaire was designed and applied. A total of 512 faecal samples were collected from 49 flocks, and 49 replies to the questionnaire were received. Parasites were identified in 100% of the flocks, and in 97% of the samples. The genera or species that have been morphologically identified were: strongyle-type, Nematodirus spp., Skrjabinema spp., Moniezia expansa, Moniezia benedeni, Trichuris spp., Capillaria spp., Eimeria spp., Dicrocoelium spp. and Fasciola hepatica. This is the first report in Portugal of Skrjabinema spp. The burden of parasites’ oocysts and eggs per gram in faecal samples ranged, respectively, from 50 to 17,550 for Eimeria spp., and from 50 to 6250 for strongyle-type eggs. Factors affecting parasitic infections were evaluated using a multivariate logistic regression. Grazing time and a lack of anthelmintic treatment were positively associated with Nematodirus spp. infection. This study showed that there is a high prevalence and diversity of gastrointestinal parasites in the Churra Galega Mirandesa sheep breed.

Statistical Modelling Techniques

Biometrics ◽  
1982 ◽  
Vol 38 (3) ◽  
pp. 871
Author(s):  
Paul Davies ◽  
S. S. Shapiro ◽  
A. J. Gross
Keyword(s):  
Statistical Modelling ◽  
Modelling Techniques
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