scholarly journals Comparison of an enzyme immuno assay (Antigenz Chlamydia) with cell culture for the detection of genital chlamydial infection in high and low risk populations.

1993 ◽  
Vol 69 (2) ◽  
pp. 119-122
Author(s):  
G Mumtaz ◽  
S Clark ◽  
G L Ridgway ◽  
C J Miller ◽  
B Johal ◽  
...  
Keyword(s):  
Cell Culture ◽  
Chlamydial Infection ◽  
Low Risk ◽  
Immuno Assay ◽  
Enzyme Immuno Assay

Chlamydia Trachomatis Antigen Detection by Chlamydiazyme Combined with Chlamydia Blocking Reagent Verification

1992 ◽  
Vol 3 (5) ◽  
pp. 355-359 ◽  
Author(s):  
Barbro Zeeberg ◽  
Ingrid Thelin ◽  
Claes Schalén
Keyword(s):  
Chlamydial Infection ◽  
Direct Immunofluorescence ◽  
Gram Negative Bacteria ◽  
Routine Laboratory ◽  
Laboratory Setting ◽  
Gram Negative ◽  
Increased Sensitivity ◽  
Immuno Assay ◽  
Enzyme Immuno Assay ◽  
Chlamydial Antigen

Several options exist for the detection of chlamydial infection in a routine laboratory setting. Enzyme immuno assay (EIA) technology offers rapid turn around of results and is less technically demanding than chlamydial cell culture. In addition, recently introduced EIA confirmatory reagents have the potential to improve the accuracy of EIA detection. We have evaluated one such confirmatory reagent (Chlamydia Blocking Reagent®, Abbott Laboratories) to determine the accuracy of the Chlamydiazyme® EIA with special regard to interpretation of low absorbance values. An initial series of 192 male urethral specimens showed that use of a lowered cut off level (absorbance value 0.05) compared with that recommended by the manufacturer increased sensitivity of the EIA from 0.73 to 0.83, thus motivating studies on this interpretative modification. Of 1101 EIA reactive specimens, 65% were determined to be chlamydia positive by the Chlamydia Blocking Reagent. The proportion of female cervical specimens that did not confirm positive was elevated compared with male urethral specimens, 43% vs. 5.7% respectively. In samples yielding absorbance from the recommended cut off level to 0.05 (approximately 50% below), the corresponding figures were 78% and 14% respectively. In 85 selected EIA reactive samples, examination by a direct immunofluorescence staining assay (DFA) (MicroTrak®, Syva Inc.) revealed elementary bodies in 85% of 67 blocking test positive and in 24% of 18 blocking test negative samples. The possibility that Gram-negative bacteria were responsible for unconfirmed EIA reactive specimens was investigated using bacterial suspensions. While EIA reactivity was noted with several strains for Gram-negative bacteria, both the blocking reagent and DFA correctly verified the absence of chlamydial antigen. We conclude that confirming Chlamydiazyme initial reactive samples with the Chlamydia Blocking Reagent improved the accuracy of chlamydial detection by identifying false positive specimens. Furthermore, by including confirmatory testing, a lower cut off level than recommended by the manufacturer seems applicable, at least for male urethral samples, thus increasing sensitivity of the Chlamydiazyme EIA.

Application of EM to differentiate herpes virus from pox virus in genital specimens

1994 ◽  
Vol 52 ◽  
pp. 262-263
Author(s):  
K. Rekrut ◽  
K. Schleuter
Keyword(s):  
Tissue Culture ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Fresh Tissue ◽  
Culture Cells ◽  
Viral Transport ◽  
Carbon Coated ◽  
Immuno Assay ◽  
Simplex Virus ◽  
Enzyme Immuno Assay

Confirmation of herpes simplex virus (HSV) from genital lesions of obstetrical (OB) patients may affect both the management of the delivery and of the neonate.(l,2) During 1992 and 1993, 4,450 genital specimens from OB patients were submitted in viral transport media for herpes culture. The specimens were inoculated into MRC-5, Vero, and A-549 tissue culture tubes, incubated, and examined daily for 7 days for cytopathic effect (CPE). The original specimens were frozen at −70° C until final reports were issued. Culture tubes with CPE were tested by the Dupont Herpchek enzyme immuno assay (EIA) to confirm the presence of herpes simplex virus (HSV). (3,4) 170 OB patient specimens were positive by culture and confirmed by EIA.There were also 63 cultures exhibiting CPE ressembling HSV which were negative by EIA testing, which failed to pass in fresh tissue culture cells or progress to more enhanced CPE in culture. These original specimens were screened by electron microscopy after direct ultracentrifugation employing the Beckman airfuge with the EM 90 rotor on to formvar carbon-coated 300 mesh copper grids and negatively stained with 2% PTA.

11-dehydro-thromboxane B2 enzyme immuno assay, a suitable screening assay for aspirin use?

2001 ◽  
Vol 120 (5) ◽  
pp. A596-A596
Author(s):  
M LEERDAM ◽  
F HUDIG ◽  
W ROOIJEN ◽  
E SLAATS ◽  
A GERAEDTS ◽  
...  
Keyword(s):  
Thromboxane B2 ◽  
Screening Assay ◽  
Immuno Assay ◽  
Enzyme Immuno Assay

scholarly journals Measurement of toxoplasma IgM by a microparticle capture enzyme immuno-assay

1993 ◽  
Vol 38 (1) ◽  
pp. 34-37 ◽  
Author(s):  
A. V. Hall ◽  
J. D. Johnson ◽  
P. J. Stevens ◽  
N. E. Renton ◽  
R. E. Holliman
Keyword(s):  
Immuno Assay ◽  
Enzyme Immuno Assay

Increased Serum Level of 8-Hydroxy-2’-Deoxyguanosine may be an Indication of Pesticides Induced DNA Damage among Indian Farmers

2021 ◽  
Vol 11 (7) ◽  
pp. 89-98
Author(s):  
Arun Pandiyan ◽  
Summaiya Lari ◽  
Sudip Ghosh
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Serum Levels ◽  
Study Data ◽  
Exposed Group ◽  
Healthy Controls ◽  
Data Assessment ◽  
Pesticides Exposure ◽  
Immuno Assay ◽  
Enzyme Immuno Assay

The aim of the present hospital based study is to assess the serum levels of 8-hydroxy-2’-deoxyguanosine (8-OHdG) among 152 subjects. A total of 152 subjects, categorized into 3 groups: (i) pesticides exposed group (N=60), (ii) pesticide un-exposed group (N=42) and (iii) healthy controls group (N=50) were recruited for the study following the inclusion and exclusion criteria. The blood was drawn from the eligible subjects and Enzyme Immuno Assay (EIA) was performed to assess the 8-OHdG levels in serum. Appropriate statistical methods were used to analyse the study data. Assessment showed that pesticides exposed group has higher levels of serum 8-OHdG as compared to un-exposed group and healthy controls. Age and duration of exposure had an impact on the levels of serum 8-OHdG.The higher serum levels of 8-OHdG may be a marker for pesticides-induced oxidative DNA damage. Key words: pesticides; exposure; oxidative stress; 8-hydroxy-2’-deoxyguanosine (8-OHdG).

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