scholarly journals Diagnosis of genital herpes by real time PCR in routine clinical practice

2004 ◽  
Vol 80 (5) ◽  
pp. 406-410 ◽  
Author(s):  
M Ramaswamy
Keyword(s):  
Clinical Practice ◽  
Real Time ◽  
Genital Herpes ◽  
Real Time Pcr ◽  
Routine Clinical Practice

scholarly journals Detection of HLA-B*57:01 by real-time PCR: implementation into routine clinical practice and additional validation data

2014 ◽  
Vol 15 (3) ◽  
pp. 319-327 ◽  
Author(s):  
Cinzia Dello Russo ◽  
Lucia Lisi ◽  
Massimiliano Fabbiani ◽  
Dimitri Gagliardi ◽  
Iuri Fanti ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Real Time ◽  
Real Time Pcr ◽  
Routine Clinical Practice ◽  
Validation Data

scholarly journals Utility of Real-Time PCR for Diagnosis of Legionnaires' Disease in Routine Clinical Practice

2007 ◽  
Vol 46 (2) ◽  
pp. 671-677 ◽  
Author(s):  
B. M. W. Diederen ◽  
J. A. J. W. Kluytmans ◽  
C. M. Vandenbroucke-Grauls ◽  
M. F. Peeters
Keyword(s):  
Clinical Practice ◽  
Real Time ◽  
Real Time Pcr ◽  
Routine Clinical Practice ◽  
Legionnaires Disease

Real-time PCR in clinical practice: a powerful tool for evaluatingLeishmania chagasiloads in naturally infected dogs

2010 ◽  
Vol 104 (2) ◽  
pp. 137-143 ◽  
Author(s):  
R. N. da Silva ◽  
A. C. Amorim ◽  
R. M. S. S. Brandão ◽  
H. M. de Andrade ◽  
M. Yokoo ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Real Time ◽  
Real Time Pcr

scholarly journals Epidemiological situation of Q fever in sheep, goats and cattle in the Wielkopolska Voivodeship between the years 2011 and 2015, based on monitoring studies and cases from clinical practice

2017 ◽  
Vol 73 (1) ◽  
pp. 56-61
Author(s):  
Piotr Kneblewski ◽  
Jolanta Budzyk ◽  
Leslaw Szabłoński ◽  
Jan Olechnowicz ◽  
Michał Majewski ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Positive Result ◽  
Real Time ◽  
Dna Sequence ◽  
Real Time Pcr ◽  
Q Fever ◽  
Serological Tests ◽  
Veterinary Research Institute ◽  
Veterinary Research ◽  
National Veterinary Research Institute

The publication presents the results of monitoring Q fever in the Wielkopolska Voivodeship and three outbreaks disclosed as part of a clinical field practice. In five years (2011 – 2015) of examination in the Wielkopolska Voivodeship, 2,431 serological tests were carried out (1,851 in sheep, 343 in goats and 237 in cattle). Antibodies against Coxiella burneti were found three times. The first positive result in 2011 affected herds of goats and cattle and was confirmed in the reference laboratory of the National Veterinary Research Institute in Pulawy. A specific DNA sequence for Coxiella burneti by real-time PCR method was found. The farm consisted of 1,494 goats and 397 cattle. Serological tests were carried out to give positive results in 15.3% of the cattle and 5.77% of the goats from the whole herd. Breeding selection and the elimination of seropositive animals and double oxytetracycline treatment reduced the proportion of animals with a positive result to 5.53% in cattle and 0.96% in goats. After more than a year the elimination of seropositive animals and probable natural decline in antibody levels has led to the recognition of an outbreak of Q fever to be eliminated. The second positive result of the monitoring of Q fever was found in 2014 in one cow out of seven respondents, but the serological test was not confirmed in the reference, as a specific DNA sequence for Coxiella burneti was not found. The research conducted in sheep in 2015 showed the presence of antibodies against Coxiella burneti in two samples. The results were confirmed by the detection of genetic material of the pathogen by real-time PCR examination in the National Veterinary Research Institute in Pulawy. Three outbreaks of Q fever revealed in clinical practice related to bovine herds where clinical disturbances were observed in: reproduction, milk production decrease or increase in internal body temperature and symptoms of the respiratory system. The positive ELISA test results were the reason for the elimination of seropositive animals. Moreover, after the disclosure of infection two herds were vaccinated using an inactivated vaccine Coxevac (CEVA), which caused the improvement of production results and relief of clinical symptoms. It is worth mentioning that in two farms along with cattle there were fallow deer supported by staff cowman. Official monitoring tests of Q fever revealed an outbreak of the disease in a herd of goats and cattle, which lead to taking effective action to protect public health because of the zoonotic nature of this infection and epidemiological risk. In the disclosure of these clinical signs in cattle it is advisable to carry out laboratory tests for Q fever.

scholarly journals Real-Time Optical Diagnosis of Colorectal Polyps in the Routine Clinical Practice Using the NICE and WASP Classifications in a Nonacademic Setting

2019 ◽  
Vol 26 (5) ◽  
pp. 314-323 ◽  
Author(s):  
Joana Castela ◽  
Susana Mão de Ferro ◽  
Isadora Rosa ◽  
Pedro Lage ◽  
Sara Ferreira ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Real Time ◽  
Colorectal Polyps ◽  
Routine Clinical Practice ◽  
Optical Diagnosis

scholarly journals Real-Time PCR Technology for Cancer Diagnostics

2002 ◽  
Vol 48 (8) ◽  
pp. 1178-1185 ◽  
Author(s):  
Philip S Bernard ◽  
Carl T Wittwer
Keyword(s):  
Clinical Practice ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Laboratory ◽  
Melting Curve ◽  
Cancer Diagnostics ◽  
Molecular Techniques ◽  
Melting Curve Analysis ◽  
Oncology Research ◽  
Tumor Profiling

Abstract Background: Advances in the biological sciences and technology are providing molecular targets for diagnosing and treating cancer. Current classifications in surgical pathology for staging malignancies are based primarily on anatomic features (e.g., tumor-node-metastasis) and histopathology (e.g., grade). Microarrays together with clustering algorithms are revealing a molecular diversity among cancers that promises to form a new taxonomy with prognostic and, more importantly, therapeutic significance. The challenge for pathology will be the development and implementation of these molecular classifications for routine clinical practice. Approach: This article discusses the benefits, challenges, and possibilities for solid-tumor profiling in the clinical laboratory with an emphasis on DNA-based PCR techniques. Content: Molecular markers can be used to provide accurate prognosis and to predict response, resistance, or toxicity to therapy. The diversity of genomic alterations involved in malignancy necessitates a variety of assays for complete tumor profiling. Some new molecular classifications of tumors are based on gene expression, requiring a paradigm shift in specimen processing to preserve the integrity of RNA for analysis. More stable markers (i.e., DNA and protein) are readily handled in the clinical laboratory. Quantitative real-time PCR can determine gene duplications or deletions. Furthermore, melting curve analysis immediately after PCR can identify small mutations, down to single base changes. These techniques are becoming easier and faster and can be multiplexed. Real-time PCR methods are a favorable option for the analysis of cancer markers. Summary: There is a need to translate recent discoveries in oncology research into clinical practice. This requires objective, robust, and cost-effective molecular techniques for clinical trials and, eventually, routine use. Real-time PCR has attractive features for tumor profiling in the clinical laboratory.

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