scholarly journals Effect of Wnt-1 antisense RNA on the outgrowth of a mammary adenocarcinoma cell line expressing that oncogene

1996 ◽  
Vol 49 (3) ◽  
pp. M166-M169 ◽  
Author(s):  
J Polanec ◽  
Z P Pavelic ◽  
W L Myers
Keyword(s):  
Cell Line ◽  
Antisense Rna ◽  
Mammary Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Mammary Adenocarcinoma Cell

High-metastatic clones selectedin vitro from a recent spontaneous BALB/c mammary adenocarcinoma cell line

1984 ◽  
Vol 2 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Pier-Luigi Lollini ◽  
Carla De Giovanni ◽  
Vincenzo Eusebi ◽  
Giordano Nicoletti ◽  
Giorgio Prodi ◽  
...  
Keyword(s):  
Cell Line ◽  
Mammary Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Mammary Adenocarcinoma Cell

Progesterone augments proliferation induced by epidermal growth factor in a feline mammary adenocarcinoma cell line

1991 ◽  
Vol 45 (2) ◽  
pp. 196-206 ◽  
Author(s):  
Jaime F. Modiano ◽  
Yasuo Kokai ◽  
David B. Weiner ◽  
Mark J. Pykett ◽  
Peter C. Nowell ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Mammary Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Mammary Adenocarcinoma Cell ◽  
Epidermal Growth

Establishment and characterization of a new mammary adenocarcinoma cell line derived from MMTV neu transgenic mice

1998 ◽  
Vol 47 (2) ◽  
pp. 171-180 ◽  
Author(s):  
Maria Grazia Sacco ◽  
Laura Gribaldo ◽  
Ottavia Barbieri ◽  
Gino Turchi ◽  
Ileana Zucchi ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cell Line ◽  
Mammary Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Mammary Adenocarcinoma Cell

Effect of permeabilization of tight junctions on morphology of 3-cell intersection

1983 ◽  
Vol 41 ◽  
pp. 716-717
Author(s):  
Sylvie Polak-Charcon ◽  
Dorothy R. Pitelka
Keyword(s):  
Tight Junctions ◽  
Cytochalasin B ◽  
Freeze Fracture ◽  
The Other ◽  
Mammary Adenocarcinoma ◽  
Mammary Cells ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Mammary Adenocarcinoma Cell ◽  
Phosphate Buffered Saline

Tight junctions (TJ) at 3-cell intersections (3Cl) appear in freeze-fracture replicas as channels limited by 2 vertical strands in one cell's membrane, each representing the line of fusion with one of the other cells. Recent observations show in mammary cells exposed to a calcium chelator, retraction of microvilli from cell borders, starting at the 3Cl. Ca2+ depletion is also known to reduce transepithelial resistance. These observations led us to inquire whether 3Cl might be the sites most vulnerable to agents that increase junction permeability. We used the rat mammary adenocarcinoma cell line LA7 (from R. Dulbecco) to ask this question.LA7 monolayers were treated with reagents known to affect transepithelial permeability: Ca2− and Mg2+-free phosphate-buffered saline (PBS-CMF), the Ca2+ chelator EGTA (0.025%), colchicine (0.1mM), cytochalasin B (10 μg/ml), procaine (1 - 10 μg/ml), kinetin (0.4 μM), phalloidin (10-50 μM). Confluent LA7 on impermeable substrates form many domes (Fig I) as fluid transported basally is trapped between the monolayer and the plastic. Their presence is proof of TJ integrity and was the criterion used in this study. Functional effects of the treatments were measured by maintenance or change in dome numbers and morphological effects were followed by freezefracturing.

scholarly journals Effect of vitex agnus castus fruits methanol extract against murine mammary adenocarcinoma cell line (amn3) and rat embryonic fibroblast normal cell line (ref)

2019 ◽  
Vol 10 (3) ◽  
Author(s):  
Zeinat. W. Ribat ◽  
Hayder B Sahib ◽  
Amjed Haseeb Khamees ◽  
Ahmed M. Al-Shammari
Keyword(s):  
Cell Line ◽  
Methanol Extract ◽  
Calf Serum ◽  
Fibroblast Cell ◽  
Mouse Mammary Gland ◽  
Mammary Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Agnus Castus ◽  
Embryonic Fibroblast

Vitex agnus castus showed antiproliferative activity in several previous studies. Angiogenesis is one of the targets in the remediation of cancer. This study aimed to demonstrate the effect of methanol fruits extract of vitex agnus castus on mouse mammary gland adenocarcinoma cell line and rat embryonic fibroblast cell line.  The cell lines used in this study were obtained from tissue culture unit/ Iraqi Center for Cancer and Medical Genetic Researches, Al-Mustansiriyah University was maintained in RPMI- 1640 tissue media after preparing from 10% fetal calf serum, antibiotics solution and other materials to make complete growth medium. Serial solutions of vitex agnus castus, methanol crude extract have been tested on 10*4 of AMN3 and REF in each well of 96 well plates. The results of the current study showed that the concentration that inhibits fifty percent of cell line after 72 hours of the experiment (IC50) was 129 ug/ml for AMN3 and 1324ug/ml for REF cell line. The antioxidant activity of Vitex agnus castus may indicate the proliferation inhibition activity of Vitex agnus castus methanol extract. The study concluded that this extract might be of benefit if used in combination with other anti-cancer drugs as adjuvant therapy.

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