scholarly journals A Rapid and Simple Method for the Determination of Esterified Fatty Acids and for Total Fatty Acids in Blood

1953 ◽  
Vol 6 (2) ◽  
pp. 158-160 ◽  
Author(s):  
I. Stern ◽  
B. Shapiro
Keyword(s):  
Fatty Acids ◽  
Simple Method ◽  
Esterified Fatty Acids ◽  
Total Fatty Acids

Estimation of Lipid Fractions of Blood Plasma

1955 ◽  
Vol 1 (5) ◽  
pp. 317-323 ◽  
Author(s):  
Andre C Kibrick ◽  
S J Skupp
Keyword(s):  
Fatty Acids ◽  
Oil Recovery ◽  
Volumetric Method ◽  
Total Lipids ◽  
Average Value ◽  
Esterified Fatty Acids ◽  
Normal Individuals ◽  
Lipid Fractions ◽  
Total Fatty Acids ◽  
Entire Procedure

Abstract The Allen volumetric method for plasma fat has been modified by calibration to include the percentage of olive-oil recovery. The values obtained from calibration with mercury are corrected for the 125 per cent recovery of the oil. The validity of the entire procedure has been discussed. A simple system of estimating the lipid fractions of plasma has been described. Total lipids are the total of Allen fat + (25 x lipid P). Neutral fat is Allen fat-cholesterol-cholesterol fatty acids. Total fatty acids are X neutral fat + cholesterol fatty acids + (0.64 x phospholipids). The values of total fatty acids have been compared with those obtained by oxidation with dichromate and by determining the esterified fatty acids with iron perchlorate. The values of neutral fat found in 13 apparently normal individuals are shown to range from 0 to 88 and to give an average value of 28 mg./100 ml.

A Simple Method for the Determination of Lipoprotein Lipase

1968 ◽  
Vol 14 (10) ◽  
pp. 1023-1025
Author(s):  
Leonard J Stutman ◽  
Marilyn Dolliver
Keyword(s):  
Fatty Acids ◽  
Lipoprotein Lipase ◽  
Fatty Acid Esters ◽  
Simple Method ◽  
Intravenous Heparin ◽  
Dense Band ◽  
Layer Chromatography ◽  
Acid Esters

Abstract A rapid method for determining the in-vivo effect of small amounts of intravenous heparin utilizing thin-layer chromatography is presented. A dense band of stainable fatty acids appears and represents nonesterified acids after the fatty acid esters have been hydrolyzed by lipoprotein lipase and determines, therefore, whether the enzyme is activated by heparin.

Effect of storage of plasma and serum on enzymatic determination of non-esterified fatty acids

2001 ◽  
Vol 38 (3) ◽  
pp. 252-255 ◽  
Author(s):  
Luis García Menéndez ◽  
Ana L Fernández ◽  
Alfredo Enguix ◽  
Constanza Ciriza ◽  
Juan Amador
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Storage Conditions ◽  
The Other ◽  
Specific Reference ◽  
Enzymatic Method ◽  
Esterified Fatty Acids ◽  
Enzymatic Determination ◽  
The Stability

Many contradictory results have been published on the stability of total non-esterified fatty acids in blood, plasma and serum under different storage conditions. The present study was undertaken to investigate the stability of non-esterified fatty acids, measured with an enzymatic method, in samples of EDTA-treated plasma and serum under different temperature conditions. We conclude that EDTA-treated plasma and serum can both be used for analysis. Specific reference values should be established depending on the type of sample chosen. Samples that cannot be analysed immediately can be stored at -20°C for at least 14 days without significant changes in the concentration of total non-esterified fatty acids. None of the other storage conditions and periods studied are suitable for the measurement of non-esterified fatty acid concentration.

A Comparison of Gas-Liquid Chromatography and a Titration Method for the Determination of Plasma Non-Esterified Fatty Acid Levels during Pregnancy

1975 ◽  
Vol 12 (1-6) ◽  
pp. 249-251 ◽  
Author(s):  
Maureen Young ◽  
Elizabeth A. Gilmore ◽  
R. G. McDonald-Gibson ◽  
Jennifer A. Elliott
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Post Partum ◽  
Gas Liquid Chromatography ◽  
Plasma Nefa ◽  
Titration Method ◽  
Esterified Fatty Acids ◽  
Nefa Level

The level of plasma non-esterified fatty acids (NEFA) was measured by gas-liquid chromatography (GLC) and a titration method in 194 samples collected during pregnancy and from four days to 24 weeks post partum. Both techniques indicated a similar pattern of changes in plasma NEFA associated with pregnancy. The titration estimates of NEFA level were usually greater than those measured by GLC, and there was some suggestion that the disparity between the methods was increased at the end of pregnancy and was reduced at six weeks after delivery.

scholarly journals The absorption of long-chain fatty acids from the small intestine of the sheep

1968 ◽  
Vol 22 (2) ◽  
pp. 247-254 ◽  
Author(s):  
Aileen M. Lennox ◽  
G. A. Garton
Keyword(s):  
Fatty Acids ◽  
Small Intestine ◽  
Neutral Lipids ◽  
Long Chain Fatty Acids ◽  
Linseed Meal ◽  
Total Lipids ◽  
Esterified Fatty Acids ◽  
Total Fatty Acids ◽  
Long Chain ◽  
Different Parts

1. Three sheep, each of which was fitted with a rumen cannula and with two pairs of reentrant cannulas in different parts of the small intestine, were used in this study. They were fed on dried grass cubes or hay plus linseed meal and oats: an aqueous solution of polyethylene glycol (PEG) was infused continuously into the rumen.2. Total lipids were extracted from samples of the chyme entering and leaving the different lengths of the small intestine embraced by the respective cannulas. The lipids were fractionated into unesterified fatty acids, neutral lipids and phospholipids and the contribution of each fraction to the total fatty acids was determined. The samples were also analysed for their PEG content, thus affording an index of the extent to which water had been absorbed from each particular length of intestine.3. From the above findings and a knowledge of the flow-rate of the digesta, the uptake of unesterified fatty acids and the degree of dissimilation or uptake, or of both, of esterified fatty acids was calculated.4. The results indicated that, by the time the digesta reached the ileum (i.e. the distal half of the small intestine), the uptake of fatty acids was almost complete, as was also the hydrolytic release of esterified fatty acids.5. Though there were no gross differences in the overall composition of the unesterified and esterified fatty acids in different parts of the small intestine, it appeared that C18 mono-unsaturated acid, the principal unsaturated unesterified acid, was absorbed somewhat more efficiently than were the major saturated acids (palmitic acid and stearic acid).

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