scholarly journals Comparison of image analysis and flow cytometric determination of cellular DNA content.

1991 ◽  
Vol 44 (2) ◽  
pp. 147-151 ◽  
Author(s):  
C Cope ◽  
D Rowe ◽  
L Delbridge ◽  
J Philips ◽  
M Friedlander
Keyword(s):  
Image Analysis ◽  
Dna Content ◽  
Flow Cytometric ◽  
Cellular Dna

Flow cytometric analysis in colorectal carcinoma: prognostic significance of cellular DNA content

1990 ◽  
Vol 5 (4) ◽  
pp. 223-227 ◽  
Author(s):  
A. Schillaci ◽  
D. D. Tirindelli ◽  
M. Ferri ◽  
L. Teodori ◽  
F. Mauro ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Dna Content ◽  
Prognostic Significance ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Cellular Dna

scholarly journals Measurement of cellular DNA mass by flow microfluorometry with use of a biological internal standard.

1978 ◽  
Vol 26 (2) ◽  
pp. 145-148 ◽  
Author(s):  
E Tannenbaum ◽  
M Cassidy ◽  
O Alabaster ◽  
C Herman
Keyword(s):  
Dna Content ◽  
Internal Standard ◽  
Cell Populations ◽  
Experimental Cell ◽  
Flow Microfluorometry ◽  
Chicken Erythrocytes ◽  
Cellular Dna ◽  
Biological Standard

Use of a biological standard (chicken erythrocytes) mixed with experimental cell populations allows control of all variables in flow microfluorometric determination of DNA content. These variables include both staining and instrument procedures. In addition, the use of a biological standard allows determination of cellular DNA mass in unperturbed cell populations. DNA mass measured by FMF technique correlates closely with values reported in the literature that used biochemical techniques.

Flow cytometric analysis of cellular DNA content in epithelial ovarian tumor

1992 ◽  
Vol 4 (2) ◽  
pp. 33-38
Author(s):  
Hongwu Wen ◽  
Shuwen Liu ◽  
Yongyan Mai ◽  
Renying Yan ◽  
Zhuxuan Shen
Keyword(s):  
Dna Content ◽  
Ovarian Tumor ◽  
Flow Cytometric Analysis ◽  
Epithelial Ovarian Tumor ◽  
Flow Cytometric ◽  
Cellular Dna

Flow cytometric determination of DNA content in isolated nuclei of cereals

Genome ◽  
1988 ◽  
Vol 30 (4) ◽  
pp. 565-569 ◽  
Author(s):  
E. Hülgenhof ◽  
R. A. Weidhase ◽  
R. Schlegel ◽  
A. Tewes
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Internal Standard ◽  
Aegilops Speltoides ◽  
Isolated Nuclei ◽  
Triticum Timopheevi ◽  
Flow Cytometric ◽  
Nuclear Isolation ◽  
Cereal Plants

Isolated nuclei from cereal plants were used for quantitative determination of DNA per nucleus by flow cytometry. The technique is based on enhanced fluorescence of ethidium bromide and olivomycin when they bind to DNA. Nuclei were isolated from protoplasts derived from leaves of seedlings. The diploid Hordeum vulgare cv. Trumpf was used as an internal standard. Analysis of nuclei from several cultivars of hexaploid wheat (Triticum aestivum), Triticum durum var. hordeiforme, T. durum var. alexandrinum, Triticum araraticum, Triticum timopheevi, Triticum monococcum, Aegilops speltoides, Secale cereale, and one hexaploid and one octoploid triticale revealed significant intra- and inter-specific differences in DNA values per 2C nucleus. The advantages of the procedure are discussed along with its utilization for quantitative DNA measurement in Gramineae.Key words: nuclear isolation, flow cytometry, DNA content, cereals.

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