scholarly journals Mechanisms of staurosporine induced apoptosis in a human corneal endothelial cell line

2003 ◽  
Vol 87 (3) ◽  
pp. 346-352 ◽  
Author(s):  
G Thuret
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Corneal Endothelial Cell ◽  
Endothelial Cell Line ◽  
Induced Apoptosis

Effects of Phthalates on the Human Corneal Endothelial Cell Line B4G12

2012 ◽  
Vol 31 (4) ◽  
pp. 364-371 ◽  
Author(s):  
Tanja Krüger ◽  
Yi Cao ◽  
Søren K. Kjærgaard ◽  
Lisbeth E. Knudsen ◽  
Eva C. Bonefeld-Jørgensen
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Endothelial Cell ◽  
Cell Line ◽  
Corneal Endothelial Cell ◽  
Industrial Chemicals ◽  
Cell Secretion ◽  
Endothelial Cell Line ◽  
Butyl Phthalate

Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di- n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di- n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.

Development and Characterization of a Rabbit Corneal Endothelial Cell Line

2004 ◽  
Vol 48 (5) ◽  
pp. 454-459 ◽  
Author(s):  
Jun Seop Shin ◽  
In Keun Jang ◽  
Chan Wha Kim ◽  
Jae Chan Kim
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Corneal Endothelial Cell ◽  
Endothelial Cell Line

scholarly journals Statin-induced apoptosis of vascular endothelial cells is blocked by dexamethasone

2002 ◽  
Vol 174 (1) ◽  
pp. 7-16 ◽  
Author(s):  
CJ Newton ◽  
G Ran ◽  
YX Xie ◽  
D Bilko ◽  
CH Burgoyne ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Survival ◽  
Cell Line ◽  
De Novo ◽  
Vascular Endothelial Cells ◽  
Pcr Analysis ◽  
Vascular Endothelial ◽  
Endothelial Cell Line ◽  
Induced Apoptosis

Statins block de novo synthesis of cholesterol by inhibiting the enzyme, HMG CoA reductase. The product of this reaction, mevalonic acid, is also a precursor of isoprenoids, molecules required for the activation of signalling G-proteins, such as Ras. Signal transduction pathways involving Ras are important for cell survival and this may be why statins induce apoptotic death of several cell types. Given that statins are used to treat vascular disease, it is surprising that no studies have been conducted on vascular endothelial cells. For this reason, we have tested the effect of fluvastatin (FS) on the endothelial cell line EA.hy 926. Here we show that FS, at concentrations from 1 to 2 microM, blocks growth and induces apoptosis of the endothelial cell line, EA.hy 926. As considerable redundancy exists in cell signalling pathways for cell survival, toxicity of FS under more physiological conditions might be prevented by pathways that do not require Ras, such as those activated by adrenal or sex steroids. To test this hypothesis, first RT-PCR analysis was performed for nuclear receptor mRNA expression. This revealed the presence of mRNA for the androgen receptor (AR) and glucocorticoid receptor (GR). The effect of the AR agonist, dihydrotestosterone (DHT), and the GR agonist, dexamethasone (Dex), was then tested. Whilst DHT (100 nM) had no effect on FS-induced cell death, Dex (1 microM) blocked FS-induced apoptosis. Cell cycle analysis revealed that 24 h exposure to FS prevented cells from leaving G(1) and 24-48 h later a marked sub-G(1) peak was observed. Dex was able to reduce the sub-G(1) peak, but it failed to reduce accumulation of cells in G(1). Further studies revealed that, in addition to blocking FS-induced apoptosis, Dex was able to block apoptosis of EA.hy 926 cells induced by serum deprivation, tumour necrosis factor-alpha, oxidants, DNA damage and mitochondrial disruption. This study strongly suggests that glucocorticoids have a role to play in preventing vascular injury and they may provide a reason why statins are apparently not toxic to vascular endothelial cells in vivo.

scholarly journals Depletion ofSLC4A11Causes Cell Death by Apoptosis in an Immortalized Human Corneal Endothelial Cell Line

2012 ◽  
Vol 53 (7) ◽  
pp. 3270 ◽  
Author(s):  
Jun Liu ◽  
Li-Fong Seet ◽  
Li Wei Koh ◽  
Anandalakshmi Venkatraman ◽  
Divya Venkataraman ◽  
...  
Keyword(s):  
Cell Death ◽  
Endothelial Cell ◽  
Cell Line ◽  
Corneal Endothelial Cell ◽  
Endothelial Cell Line

Establishment of a novel corneal endothelial cell line from domestic rabbit, Oryctolagus curiculus

2007 ◽  
Vol 50 (2) ◽  
pp. 161-169 ◽  
Author(s):  
TingJun Fan ◽  
Jun Zhao ◽  
YongFeng Fu ◽  
RiShan Cong ◽  
RuiChao Guo ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Corneal Endothelial Cell ◽  
Endothelial Cell Line ◽  
Domestic Rabbit

scholarly journals Establishment of Functioning Human Corneal Endothelial Cell Line with High Growth Potential

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e29677 ◽  
Author(s):  
Tadashi Yokoi ◽  
Yuko Seko ◽  
Tae Yokoi ◽  
Hatsune Makino ◽  
Shin Hatou ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
High Growth ◽  
Corneal Endothelial Cell ◽  
Growth Potential ◽  
Endothelial Cell Line ◽  
High Growth Potential
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