scholarly journals Amniotic membrane transplantation for reconstruction after excision of large ocular surface neoplasias

2002 ◽  
Vol 86 (6) ◽  
pp. 640-645 ◽  
Author(s):  
E M Espana
Keyword(s):  
Amniotic Membrane ◽  
Ocular Surface ◽  
Amniotic Membrane Transplantation

scholarly journals Nano-Scale Modifications of Amniotic Membrane Induced by UV and Antibiotic Treatment: Histological, AFM and FTIR Spectroscopy Evidence

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 863
Author(s):  
Simona Cavalu ◽  
George Roiu ◽  
Ovidiu Pop ◽  
Denisa A. Petricas Heredea ◽  
Traian Octavian Costea ◽  
...  
Keyword(s):  
Ftir Spectroscopy ◽  
Antibiotic Treatment ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Enzymatic Digestion ◽  
Uv Exposure ◽  
Amniotic Membrane Transplantation ◽  
Corneal Tissue ◽  
Nano Scale ◽  
Gentamicin Treatment

The efficiency of amniotic membrane (AM) transplantation in different types of ocular surface disorders is due to its outstanding properties such as antifibrotic, antibacterial, anti-inflammatory and antiangiogenic, working as a versatile scaffold to promote corneal tissue epithelialization. A proper preparation, preservation and clinical application are crucial for the best outcomes in the treatment of different severe ocular disorders, taking into account its fragility. In this context, by combining high-sensitivity tools such as atomic force microscopy (AFM) and Fourier transform infrared (FTIR) spectroscopy with histological and immunohistochemical examination, we aimed to investigate the ultrastructural modifications of the amniotic membrane (AM) upon UV exposure and/or antibiotic treatment, with relevance for clinical applications in ocular surface surgery. From the morphological point of view, we noticed a loss of cuboidal cells in the basal membrane, accompanied by the splitting of collagen fibers upon UV and/or gentamicin treatment, while structural alteration of proteins was evidenced by the FTIR quantitative analysis of the secondary structure. A decrease in α-helix and β-sheet content, accompanied by increased content in less ordered structures (turns, random and side chains), was noticed after all the treatments. At the nano-scale, AFM details showed modifications of collagen fibrils in terms of their thickness and network compaction upon gentamicin and/or UV treatment. The enzymatic digestion assay demonstrated that UV exposure significantly reduces the degradation rate of the AM, while gentamicin treatment promotes an accelerated enzymatic digestion upon UV exposure. In order to highlight the clinical impact of the research, a clinical case is presented showing the relevance of amniotic membrane transplantation in pterygium surgery.

scholarly journals Amniotic membrane transplantation in surgical management of ocular surface squamous neoplasias: long-term results

Eye ◽  
2014 ◽  
Vol 28 (9) ◽  
pp. 1131-1135 ◽  
Author(s):  
M Palamar ◽  
E Kaya ◽  
S Egrilmez ◽  
T Akalin ◽  
A Yagci
Keyword(s):  
Surgical Management ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Long Term Results ◽  
Amniotic Membrane Transplantation

Amniotic Membrane Transplantation for Ocular Surface Reconstruction

2001 ◽  
Vol 21 (4) ◽  
pp. 481-489 ◽  
Author(s):  
S.C.G. Tseng
Keyword(s):  
Surface Reconstruction ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Transforming Growth Factor ◽  
Ease Of Use ◽  
Myoblast Differentiation ◽  
Amniotic Membrane Transplantation ◽  
Clinical Effects ◽  
Epithelial Stem Cells ◽  
Ocular Surface Reconstruction

The use of amniotic membrane (or amnion) for transplantation as graft in ocular surface reconstruction is reviewed. This technique has become widespread because of the availability of the amnion, convenience and ease of use, and high and reproducible success rates. The mechanisms of action of the transplantation are varied and include the prolongation and clonogenic maintenance of epithelial progenitor cells, promotion of goblet and non-goblet cell differentiation, exclusion of inflammatory cells with anti-protease activities, suppression of Transforming Growth Factor β signaling and myoblast differentiation of normal fibroblasts. The observed clinical effects include facilitation of epithelialization, maintenance of normal phenotypes, and reduction of inflammation, vascularization and scarring. Amniotic membrane transplantation is being increasingly used as graft for various conjunctival and corneal diseases and as a patch in cases of chemical and thermal burns, refractory and recalcitrant keratitis, and most recently as an excellent substrate for expanding epithelial stem cells ex vivo.

Amniotic membrane transplantation for ocular surface reconstruction

1998 ◽  
Vol 95 (2) ◽  
pp. 114-119 ◽  
Author(s):  
Friedrich E. Kruse ◽  
Klaus Rohrschneider ◽  
Hans E. Völcker
Keyword(s):  
Surface Reconstruction ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Amniotic Membrane Transplantation ◽  
Ocular Surface Reconstruction

scholarly journals Tenonplasty Combined With Amniotic Membrane Transplantation for Patients With Severe Ocular Burns Induced Anterior Segment Necrosis

2020 ◽  
Vol 41 (3) ◽  
pp. 668-673
Author(s):  
Wen-yan Peng ◽  
Li-wen He ◽  
Peng Zeng ◽  
Dong-cui Chen ◽  
Shi-you Zhou
Keyword(s):  
Amniotic Membrane ◽  
Ocular Surface ◽  
Anterior Segment ◽  
Case Series ◽  
Corneal Opacity ◽  
Vascular Supply ◽  
Amniotic Membrane Transplantation ◽  
Final Visit ◽  
Prospective Case Series ◽  
Ocular Burns

Abstract This article describes a novel surgical technique for successful repair of intractable corneoscleral necrosis caused by severe ocular burns. In this prospective case series, 19 eyes of 15 consecutive patients with sectional scleral necrosis and persistent corneal epithelial defects were treated with tenonplasty and amniotic membrane transplantation. The main outcome measure was the stability of the ocular surface after reepithelialization and repair of defects. All patients underwent successful combined surgery involving tenonplasty and amniotic membrane transplantation, in which the conjunctival and corneal surfaces were reconstructed. The interval from injury to surgery was 37.4 ± 24.5 days (3–91 days), and the ocular surfaces became stabilized in 82.2 ± 35.4 days (26–156 days, median 87 days). At the final visit, all cases presented with corneal opacity and neovascularization to various degrees. The best-corrected visual acuity decreased from 2.83 ± 1.02 LogMAR preoperatively to 2.87 ± 1.31 LogMAR postoperatively. The results imply that tenonplasty combined with amniotic membrane transplantation could provide vascular supply to the ischemic sclera, repair defects in the conjunctiva, and promote corneal reepithelialization, thus facilitating ocular surface stabilization after burns.

Sutureless amniotic membrane transplantation for ocular surface reconstruction with a chemically defined bioadhesive

Biomaterials ◽  
2008 ◽  
Vol 29 (19) ◽  
pp. 2923-2931 ◽  
Author(s):  
Maho Takaoka ◽  
Takahiro Nakamura ◽  
Hajime Sugai ◽  
Adam J. Bentley ◽  
Naoki Nakajima ◽  
...  
Keyword(s):  
Surface Reconstruction ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Amniotic Membrane Transplantation ◽  
Ocular Surface Reconstruction
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