scholarly journals Amniotic membrane transplantation in surgical management of ocular surface squamous neoplasias: long-term results

Eye ◽  
2014 ◽  
Vol 28 (9) ◽  
pp. 1131-1135 ◽  
Author(s):  
M Palamar ◽  
E Kaya ◽  
S Egrilmez ◽  
T Akalin ◽  
A Yagci
Keyword(s):  
Surgical Management ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Long Term Results ◽  
Amniotic Membrane Transplantation

Amniotic membrane transplantation for ocular surface pathology: long-term results

2003 ◽  
Vol 35 (5) ◽  
pp. 2031-2035 ◽  
Author(s):  
O Gris ◽  
A López-Navidad ◽  
F Caballero ◽  
Z del Campo ◽  
A Adán
Keyword(s):  
Amniotic Membrane ◽  
Ocular Surface ◽  
Long Term Results ◽  
Amniotic Membrane Transplantation

Long-Term Results of Amniotic Membrane Transplantation for Partial Limbal Deficiency

Cornea ◽  
2013 ◽  
Vol 32 (8) ◽  
pp. 1110-1115 ◽  
Author(s):  
Kenji Konomi ◽  
Yoshiyuki Satake ◽  
Shigeto Shimmura ◽  
Kazuo Tsubota ◽  
Jun Shimazaki
Keyword(s):  
Amniotic Membrane ◽  
Long Term Results ◽  
Amniotic Membrane Transplantation ◽  
Limbal Deficiency

Short- and Long-Term Results of the Surgical Management of Frontobasal Fractures

Skull Base ◽  
2007 ◽  
Vol 17 (S 1) ◽  
Author(s):  
Boštjan Lanišnik ◽  
Vojko Didanovič ◽  
Bogdan Čizmarevič
Keyword(s):  
Surgical Management ◽  
Long Term Results ◽  
Short And Long Term

scholarly journals Nano-Scale Modifications of Amniotic Membrane Induced by UV and Antibiotic Treatment: Histological, AFM and FTIR Spectroscopy Evidence

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 863
Author(s):  
Simona Cavalu ◽  
George Roiu ◽  
Ovidiu Pop ◽  
Denisa A. Petricas Heredea ◽  
Traian Octavian Costea ◽  
...  
Keyword(s):  
Ftir Spectroscopy ◽  
Antibiotic Treatment ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Enzymatic Digestion ◽  
Uv Exposure ◽  
Amniotic Membrane Transplantation ◽  
Corneal Tissue ◽  
Nano Scale ◽  
Gentamicin Treatment

The efficiency of amniotic membrane (AM) transplantation in different types of ocular surface disorders is due to its outstanding properties such as antifibrotic, antibacterial, anti-inflammatory and antiangiogenic, working as a versatile scaffold to promote corneal tissue epithelialization. A proper preparation, preservation and clinical application are crucial for the best outcomes in the treatment of different severe ocular disorders, taking into account its fragility. In this context, by combining high-sensitivity tools such as atomic force microscopy (AFM) and Fourier transform infrared (FTIR) spectroscopy with histological and immunohistochemical examination, we aimed to investigate the ultrastructural modifications of the amniotic membrane (AM) upon UV exposure and/or antibiotic treatment, with relevance for clinical applications in ocular surface surgery. From the morphological point of view, we noticed a loss of cuboidal cells in the basal membrane, accompanied by the splitting of collagen fibers upon UV and/or gentamicin treatment, while structural alteration of proteins was evidenced by the FTIR quantitative analysis of the secondary structure. A decrease in α-helix and β-sheet content, accompanied by increased content in less ordered structures (turns, random and side chains), was noticed after all the treatments. At the nano-scale, AFM details showed modifications of collagen fibrils in terms of their thickness and network compaction upon gentamicin and/or UV treatment. The enzymatic digestion assay demonstrated that UV exposure significantly reduces the degradation rate of the AM, while gentamicin treatment promotes an accelerated enzymatic digestion upon UV exposure. In order to highlight the clinical impact of the research, a clinical case is presented showing the relevance of amniotic membrane transplantation in pterygium surgery.

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