scholarly journals An estimate of the size and shape of the human lens fibre in vivo.

1987 ◽  
Vol 71 (12) ◽  
pp. 916-922 ◽  
Author(s):  
N A Brown ◽  
A J Bron ◽  
J M Sparrow
Keyword(s):  
Human Lens ◽  
Size And Shape ◽  
Lens Fibre

scholarly journals In vivo carbamylation and acetylation of water-soluble human lens αB-crystallin lysine 92

2001 ◽  
Vol 10 (6) ◽  
pp. 1130-1136 ◽  
Author(s):  
Veniamin N. Lapko ◽  
David L. Smith ◽  
Jean B. Smith
Keyword(s):  
Water Soluble ◽  
Human Lens ◽  
Αb Crystallin

Assigning in vivo carbamylation and acetylation in human lens proteins using tandem mass spectrometry and database searching

2007 ◽  
Vol 259 (1-3) ◽  
pp. 161-173 ◽  
Author(s):  
Zee-Yong Park ◽  
Rovshan Sadygov ◽  
Judy M. Clark ◽  
John I. Clark ◽  
John R. Yates
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Human Lens ◽  
Database Searching ◽  
Tandem Mass ◽  
Lens Proteins

In Vivo Measurements On Human Lens Using Quasielastic Light Scattering

1987 ◽  
Author(s):  
Peter C. Magnante ◽  
Leo T. Chylack ◽  
George B. Benedek ◽  
Teodosio Libondi ◽  
Stephen N. Joffe ◽  
...  
Keyword(s):  
Light Scattering ◽  
Human Lens ◽  
Quasielastic Light Scattering ◽  
In Vivo Measurements

scholarly journals In Vivo Quasi-Elastic Light Scattering Eye Scanner Detects Molecular Aging in Humans

2020 ◽  
Vol 75 (9) ◽  
pp. e53-e62
Author(s):  
Olga Minaeva ◽  
Srikant Sarangi ◽  
Danielle M Ledoux ◽  
Juliet A Moncaster ◽  
Douglas S Parsons ◽  
...  
Keyword(s):  
Light Scattering ◽  
Human Lens ◽  
Fetal Life ◽  
Healthy Human ◽  
Elastic Light Scattering ◽  
Lens Proteins ◽  
Age Dependent ◽  
Molecular Aging

Abstract The absence of clinical tools to evaluate individual variation in the pace of aging represents a major impediment to understanding aging and maximizing health throughout life. The human lens is an ideal tissue for quantitative assessment of molecular aging in vivo. Long-lived proteins in lens fiber cells are expressed during fetal life, do not undergo turnover, accumulate molecular alterations throughout life, and are optically accessible in vivo. We used quasi-elastic light scattering (QLS) to measure age-dependent signals in lenses of healthy human subjects. Age-dependent QLS signal changes detected in vivo recapitulated time-dependent changes in hydrodynamic radius, protein polydispersity, and supramolecular order of human lens proteins during long-term incubation (~1 year) and in response to sustained oxidation (~2.5 months) in vitro. Our findings demonstrate that QLS analysis of human lens proteins provides a practical technique for noninvasive assessment of molecular aging in vivo.

scholarly journals In vivo acetylation identified at lysine 70 of human lens αA-crystallin

1998 ◽  
Vol 7 (6) ◽  
pp. 1451-1457 ◽  
Author(s):  
Peiping P. Lin ◽  
Richard C. Barry ◽  
David L. Smith ◽  
Jean B. Smith
Keyword(s):  
Human Lens

Age-Related Changes of Human Lens Epithelial Cells in vivo

2001 ◽  
Vol 33 (6) ◽  
pp. 363-366 ◽  
Author(s):  
Hideaki Oharazawa ◽  
Nobuhiro Ibaraki ◽  
Hironori Matsui ◽  
Kunitoshi Ohara
Keyword(s):  
Epithelial Cells ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Age Related ◽  
Age Related Changes

scholarly journals Transcriptomic analysis and novel insights into lens fibre cell differentiation regulated by Gata3

Open Biology ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 190220
Author(s):  
Elena Martynova ◽  
Yilin Zhao ◽  
Qing Xie ◽  
Deyou Zheng ◽  
Ales Cvekl
Keyword(s):  
Cell Differentiation ◽  
Transcriptional Control ◽  
Kinase Inhibitors ◽  
Neuroendocrine Cells ◽  
Intermediate Filament Protein ◽  
Loss Of Function ◽  
Fibre Cell ◽  
Lens Fibre ◽  
Lens Fibre Cell

Gata3 is a DNA-binding transcription factor involved in cellular differentiation in a variety of tissues including inner ear, hair follicle, kidney, mammary gland and T-cells. In a previous study in 2009, Maeda et al . ( Dev. Dyn. 238 , 2280–2291; doi:10.1002/dvdy.22035 ) found that Gata3 mutants could be rescued from midgestational lethality by the expression of a Gata3 transgene in sympathoadrenal neuroendocrine cells. The rescued embryos clearly showed multiple defects in lens fibre cell differentiation. To determine whether these defects were truly due to the loss of Gata3 expression in the lens, we generated a lens-specific Gata3 loss-of-function model. Analogous to the previous findings, our Gata3 null embryos showed abnormal regulation of cell cycle exit during lens fibre cell differentiation, marked by reduction in the expression of the cyclin-dependent kinase inhibitors Cdkn1b/p27 and Cdkn1c/p57, and the retention of nuclei accompanied by downregulation of Dnase IIβ. Comparisons of transcriptomes between control and mutated lenses by RNA-Seq revealed dysregulation of lens-specific crystallin genes and intermediate filament protein Bfsp2. Both Cdkn1b/p27 and Cdkn1c/p57 loci are occupied in vivo by Gata3, as well as Prox1 and c-Jun, in lens chromatin. Collectively, our studies suggest that Gata3 regulates lens differentiation through the direct regulation of the Cdkn1b/p27and Cdkn1c/p57 expression, and the direct/or indirect transcriptional control of Bfsp2 and Dnase IIβ.

FGF suppresses apoptosis and induces differentiation of fibre cells in the mouse lens

Development ◽  
1995 ◽  
Vol 121 (12) ◽  
pp. 4383-4393 ◽  
Author(s):  
R.L. Chow ◽  
G.D. Roux ◽  
M. Roghani ◽  
M.A. Palmer ◽  
D.B. Rifkin ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transgenic Mice ◽  
Dominant Negative ◽  
Fgf Receptor ◽  
Fibre Cell ◽  
Dominant Negative Form ◽  
Lens Fibre ◽  
Mouse Lens ◽  
Negative Form

To determine whether fibroblast growth factor (FGF) has a role in lens development, we have generated transgenic mice expressing a dominant-negative form of the murine FGF receptor-1 (FGFRDN) in the lens. Using the fibre cell-specific alpha A-crystallin promoter to express the FGFRDN, we have asked whether FGF is required for fibre cell differentiation. The transgenic mice display diminished differentiation of fibre cells as indicated by their reduced elongation. In addition, transgenic lenses have an unusual refractile anomaly that morphological and biochemical data show results from the apoptosis of fibre cells in the central region of the lens. These results show that lens fibre cells are dependent on FGF for their survival and differentiation, and demonstrate that growth factor deprivation in vivo can lead to apoptosis.

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