Bioactive peptides with mitogenic activity in goat and sheep amniotic fluid

2001 ◽  
Author(s):  
Ján Blahovec ◽  
Zuzana Kostecká ◽  
Francoise Cavaille ◽  
Marie Lacroix ◽  
Ján Mester
Keyword(s):  
Amniotic Fluid ◽  
Bioactive Peptides ◽  
Mitogenic Activity

scholarly journals Insulin-like growth factor binding proteins and mitogenic activity of partially fractionated sheep amniotic fluid

2001 ◽  
Vol 49 (1) ◽  
pp. 65-70 ◽  
Author(s):  
J. Blahovec ◽  
Zuzana Kostecká ◽  
Françoise Cavaille ◽  
M. G. Lacroix ◽  
J. Mester
Keyword(s):  
Growth Factor ◽  
Amniotic Fluid ◽  
Molecular Mass ◽  
Binding Proteins ◽  
Mitogenic Activity ◽  
Insulin Like Growth Factor ◽  
Igf Binding Proteins ◽  
Concentration Peak ◽  
Igf Binding ◽  
Two Peaks

Amniotic fluid collected from ewes on various days of gestation was examined for the presence of insulin-like growth factor (IGF) binding proteins. IGF-binding proteins with a molecular mass of 40–45 kDa appeared at day 41 of gestation. The level of these major IGF-binding proteins increased during pregnancy and reached a maximum at day 106. Smaller IGF-binding molecules with an approximate molecular mass of 35 kDa and 25 kDa appeared at day 90, also reaching a concentration peak at day 106. The mitogenic activity of sheep amniotic fluid after chromatography on Sephadex G-50 was separated into two peaks. The peak having lower molecular mass corresponded to an elution profile of 125I-IGF-I. The first peak, having higher molecular mass, was eluted immediately after the void volume of column. Electrophoresis and ligand blotting showed that proteins in the first peak had similar properties as IGF-binding proteins.

scholarly journals Peptidomic Analysis of Amniotic Fluid for Identification of Putative Bioactive Peptides in Ventricular Septal Defect

2016 ◽  
Vol 38 (5) ◽  
pp. 1999-2014 ◽  
Author(s):  
Xing Li ◽  
Li-Jie Wu ◽  
Meng Gu ◽  
Yu-Mei Chen ◽  
Qi-Jun Zhang ◽  
...  
Keyword(s):  
Ventricular Septal Defect ◽  
Amniotic Fluid ◽  
Congenital Heart ◽  
Bioactive Peptides ◽  
Septal Defect ◽  
Heart Diseases ◽  
Tandem Mass ◽  
Cardiovascular Development ◽  
Human Amniotic Fluid ◽  
Liquid Chromatography Tandem Mass

Background: Ventricular septal defect (VSD) is one of the most common congenital heart diseases and to date the role of peptides in human amniotic fluid in the pathogenesis of VSD have been rarely investigated. Methods: To gain insight into the mechanisms of protein and peptides in cardiovascular development, we constructed a comparative peptidomic profiling of human amniotic fluid between normal and VSD fetuses using a stable isobaric labeling strategy involving tandem mass tag reagents, followed by nano liquid chromatography tandem mass spectrometry. Results: We identified and quantified 692 non-redundant peptides, 183 of which were differentially expressed in the amniotic fluid of healthy and VSD fetuses; 69 peptides were up regulated and 114 peptides were down regulated. These peptides were imported into the Ingenuity Pathway Analysis (IPA) and identified putative roles in cardiovascular system morphogenesis and cardiogenesis. Conclusion: We concluded that 35 peptides located within the functional domains of their precursor proteins could be candidate bioactive peptides for VSD. The identified peptide changes in amniotic fluid of VSD fetuses may advance our current understanding of congenital heart disease and these peptides may be involved in the etiology of VSD.

scholarly journals Mitogenic activity of high molecular weight forms of insulin-like growth factor-II in amniotic fluid

2001 ◽  
Vol 169 (3) ◽  
pp. 563-572 ◽  
Author(s):  
J Blahovec ◽  
Z Kostecka ◽  
MC Lacroix ◽  
L Cabanie ◽  
F Godeau ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Mitogenic Activity ◽  
Size Exclusion ◽  
Igf Binding Proteins ◽  
Foetal Development ◽  
Factor Ii ◽  
Exclusion Chromatography ◽  
Igf Binding ◽  
Two Peaks

Amniotic fluid (AF) collected from ewes and goats at mid gestation displayed mitogenic activity in mouse fibroblasts. Upon fractionation of this material by size exclusion chromatography, the mitogenic activity was resolved into two peaks, whose activity was inhibited by an anti-IGF type 1 receptor blocking antibody. One of the peaks contained IGF-I and IGF-II (mature form), whereas the other contained high M(r) precursor forms of IGF-II. The presence in this latter fraction of IGF-binding proteins (IGFBP) suggests that the AF IGFBPs do not efficiently inhibit the mitogenic activity of the high M(r) forms of IGF-II. In agreement with this conclusion, exogenous IGFBP-1 failed to affect this activity. Analysis of IGF-II in sheep AF showed that the AF concentrations of both forms of IGF-II increased dramatically from mid pregnancy until 106-120 days of gestation, and fell thereafter. The amniotic IGFBPs followed a similar evolution. High M(r) forms of IGF-II were also found in human AF, with a pattern of electrophoretic migration different from that of sheep. We suggest that the precursor forms of IGF-II may play an important role in foetal development.

scholarly journals Influence of Ruminant Amniotic Fluid Fractions on Fibroblast and Lymphocyte Proliferation

2006 ◽  
Vol 75 (2) ◽  
pp. 169-174 ◽  
Author(s):  
Z. Kostecká ◽  
A. Sobeková ◽  
J. Blahovec
Keyword(s):  
Amniotic Fluid ◽  
Thymidine Incorporation ◽  
Colorimetric Assay ◽  
Cell Types ◽  
Peripheral Blood Lymphocytes ◽  
Mitogenic Activity ◽  
Mtt Colorimetric Assay ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Selection Of

Different substances of amniotic fluid influence the cell proliferation and differentiation of developing animal fetus. The aim of this study was to determine the mitogenic effect of some peptide components of bovine amniotic fluid on bovine peripheral blood lymphocytes using methyl tetrazolium (MTT) colorimetric assay. The next aim of our work was to determine the mitogenic activity of ovine amniotic fluid fractions of peptide nature on benzo-á-pyrene transformed BALB/c 3T3 mouse fibroblasts (BPA31 cells) by use of 3H-thymidine incorporation into nucleus DNA and in conclusion, to compare the mitogenic activity of ruminant amniotic fluid fractions on different indicator cells. According to our study, inhibiting effect was found only in the case of separated bovine amniotic fluid (Peak I) and ovine amniotic fluid (B fraction). On the other hand, we have observed activation of lymphocytes by other fraction of bovine amniotic fluid (Peak II) and also of BP- A31 cells by fraction A in case of ovine amniotic fluid. The proliferation of peripheral lymphocytes was not significantly changed after the addition of natural bovine amniotic fluid likewise when the delipidated ovine amniotic fluid was added to BP-A31 cells, there was no effect on 3H-thymidine incorporation. Our results suggest that with testing the proliferation effect, the selection of indicator cells is of great importance since various cell types respond in different ways to the same substances.

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