Erythrocyte Membrane Fluidity in Diabetics: Fluorescence Study

1999 ◽  
Vol 64 (3) ◽  
pp. 548-552 ◽  
Author(s):  
Vladimír Jakuš ◽  
Uwe Fuhr ◽  
Wolfgang Wörner ◽  
Norbert Rietbrock
Keyword(s):  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Fluorescence Anisotropy ◽  
Erythrocyte Membranes ◽  
Fluorescence Study ◽  
Healthy Donors ◽  
Erythrocyte Membrane Fluidity ◽  
The Mean ◽  
Fluidity Of Membranes

Erythrocyte membrane fluidity is changed in diabetic subjects with long-term complications. As membrane fluidity indicator, the mean steady-state fluorescence anisotropy was measured in 1,6-diphenylhexa-1,3,5-triene labelled erythrocyte membranes prepared from six control healthy donors and six poorly controlled diabetic subjects. Fluorescence anisotropy values of membranes prepared from erythrocytes of diabetic subjects were significantly higher than in control subjects. This indicates a decreased fluidity of membranes prepared from diabetic subjects. The decreased fluidity of diabetic membranes was raised by glycation inhibitors - penicillamine, captopril, and lipoic acid.

Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study

2010 ◽  
Vol 88 (4) ◽  
pp. 497-500 ◽  
Author(s):  
Vladimir Ajdžanović ◽  
Ivan Spasojević ◽  
Branko Filipović ◽  
Branka Šošić-Jurjević ◽  
Milka Sekulić ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Electron Paramagnetic Resonance Study ◽  
Erythrocyte Membranes ◽  
Paramagnetic Resonance ◽  
Physiological Level ◽  
Luminal Diameter ◽  
Erythrocyte Membrane Fluidity ◽  
Electron Paramagnetic

The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p < 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p < 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.

Erythrocyte membrane fluidity and haemoglobin haemoporphyrin conformation: features revealed in patients with heart failure

2005 ◽  
Vol 11 (4) ◽  
pp. 209-213 ◽  
Author(s):  
O.V. Rodnenkov ◽  
O.G. Luneva ◽  
N.A. Ulyanova ◽  
G.V. Maksimov ◽  
A.B. Rubin ◽  
...  
Keyword(s):  
Heart Failure ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Patients With Heart Failure ◽  
Erythrocyte Membrane Fluidity

Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans

2010 ◽  
Vol 111 (6) ◽  
pp. 1127-1133 ◽  
Author(s):  
C. Berzosa ◽  
E. M. Gómez–Trullén ◽  
E. Piedrafita ◽  
I. Cebrián ◽  
E. Martínez–Ballarín ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Oxidative Damage ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Erythrocyte Membrane Fluidity ◽  
Acute Physical Exercise ◽  
Exercise In Humans

Mechanism of increased erythrocyte membrane fluidity during magnesium deficiency in weanling rats

1989 ◽  
Vol 257 (2) ◽  
pp. C270-C276 ◽  
Author(s):  
S. Tongyai ◽  
Y. Rayssiguier ◽  
C. Motta ◽  
E. Gueux ◽  
P. Maurois ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Magnesium Deficiency ◽  
Osmotic Fragility ◽  
Magnesium Content ◽  
Male Rats ◽  
Surface Irregularities ◽  
Erythrocyte Membrane Fluidity ◽  
And Control

The erythrocyte membrane was investigated in weanling male rats pair fed with magnesium-deficient and control diets for 8 days. Fluorescence polarization studies revealed a 15% increase in the fluidity of membranes from deficient rats. A similar increase in the fluidity of liposomes indicated that protein was not involved. The change was associated with decreased osmotic fragility of intact erythrocytes; the cells lost their biconcavity and had a flattened appearance with surface irregularities. Analysis of the membranes showed decreased amounts of magnesium, cholesterol, and sphingomyelin in the deficient group. The reduced ratios of cholesterol to phospholipid and sphingomyelin to phosphatidylcholine were consistent with the increased fluidity. Addition of physiological amounts of magnesium to the medium rigidified membranes incubated in tris(hydroxymethyl)-aminomethane buffer, and this was prevented by the presence of EDTA. Cross-incubation experiments with erythrocyte ghosts and plasma from the two groups of rats showed that magnesium-deficient plasma increased the fluidity of control ghosts and control plasma rigidified ghosts from magnesium-deficient rats. Addition of sufficient magnesium chloride to raise the magnesium content of deficient plasma to normal had no significant effect. These results show that the increased fluidity of the erythrocyte membrane in magnesium deficiency is due to physicochemical exchange with the plasma. Although magnesium can directly influence membrane fluidity, the change during its deficiency in vivo is mainly mediated indirectly via disturbances in lipid metabolism.

scholarly journals Supplementation of iron alone and combined with vitamins improves haematological status, erythrocyte membrane fluidity and oxidative stress in anaemic pregnant women

2010 ◽  
Vol 104 (11) ◽  
pp. 1655-1661 ◽  
Author(s):  
Ai Guo Ma ◽  
Evert G. Schouten ◽  
Yong Ye Sun ◽  
Fang Yang ◽  
Xiu Xia Han ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pregnant Women ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Ferrous Sulphate ◽  
Randomised Trial ◽  
Control Group ◽  
Placebo Control ◽  
Group I ◽  
Erythrocyte Membrane Fluidity

Pregnancy is a condition exhibiting increased susceptibility to oxidative stress, and Fe plays a central role in generating harmful oxygen species. The objective of the present study is to investigate the changes in haematological status, oxidative stress and erythrocyte membrane fluidity in anaemic pregnant women after Fe supplementation with and without combined vitamins. The study was a 2 months double-blind, randomised trial. Pregnant women (n 164) were allocated to four groups: group C was the placebo control group; group I was supplemented daily with 60 mg Fe (ferrous sulphate) daily; group IF was supplemented daily with Fe plus 400 μg folic acid; group IM was supplemented daily with Fe plus 2 mg retinol and 1 mg riboflavin, respectively. After the 2-month trial, Hb significantly increased by 15·8, 17·3 and 21·8 g/l, and ferritin by 2·8, 3·6 and 11·0 μg/l, in the I, IF and IM groups compared with placebo. Polarisation (ρ) and microviscosity (η) decreased significantly in other groups compared with placebo, indicating an increase in membrane fluidity. Significant decreases of ρ and η values compared with group C were 0·033 and 0·959 for group I, 0·037 and 1·074 for group IF and 0·064 and 1·865 for group IM, respectively. In addition, significant increases of glutathione peroxidase activities and decreases of malondialdehyde were shown in all treated groups, as well as increases of plasma retinol and urine riboflavin in group IM. The findings show that supplementation with Fe and particularly in combination with vitamins could improve the haematological status as well as oxidative stress and erythrocyte membrane fluidity.

Erythrocyte membrane fluidity during mesoglycan treatment

1992 ◽  
Vol 12 (3) ◽  
pp. 449-453
Author(s):  
Gregorio Caimi ◽  
Stefano Romandini ◽  
Rosalia Lo Presti ◽  
Antonio Serra ◽  
Maria Montana ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Erythrocyte Membrane Fluidity
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