Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study

2010 ◽  
Vol 88 (4) ◽  
pp. 497-500 ◽  
Author(s):  
Vladimir Ajdžanović ◽  
Ivan Spasojević ◽  
Branko Filipović ◽  
Branka Šošić-Jurjević ◽  
Milka Sekulić ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Electron Paramagnetic Resonance Study ◽  
Erythrocyte Membranes ◽  
Paramagnetic Resonance ◽  
Physiological Level ◽  
Luminal Diameter ◽  
Erythrocyte Membrane Fluidity ◽  
Electron Paramagnetic

The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p < 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p < 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.

The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study

2010 ◽  
Vol 239 (3) ◽  
pp. 131-135 ◽  
Author(s):  
Vladimir Ajdžanović ◽  
Ivan Spasojević ◽  
Branka Šošić-Jurjević ◽  
Branko Filipović ◽  
Svetlana Trifunović ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Electron Paramagnetic Resonance Study ◽  
Paramagnetic Resonance ◽  
Negative Effect ◽  
Erythrocyte Membrane Fluidity ◽  
Electron Paramagnetic

Changes of the peripheral blood mononuclear cells membrane fluidity from type 1 Gaucher disease patients: an electron paramagnetic resonance study

2018 ◽  
Vol 399 (5) ◽  
pp. 447-452 ◽  
Author(s):  
Aleksandra Pavićević ◽  
Milan Lakočević ◽  
Milan Popović ◽  
Ana Popović-Bijelić ◽  
Marko Daković ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Fluidity ◽  
Peripheral Blood ◽  
Gaucher Disease ◽  
Electron Paramagnetic Resonance Study ◽  
Peripheral Blood Mononuclear ◽  
Paramagnetic Resonance ◽  
Simple Method ◽  
Significant Difference ◽  
Electron Paramagnetic

Abstract Gaucher disease (GD) is a lysosomal storage disorder, caused by an impaired function of β-glucocerebrosidase, which results in accumulation of glucocerebroside in cells, and altered membrane ordering. Using electron paramagnetic resonance spin labeling, a statistically significant difference in the order parameter between the peripheral blood mononuclear cell membranes of GD patients and healthy controls was observed. Moreover, the results show that the introduction of the enzyme replacement therapy leads to the restoration of the physiological membrane fluidity. Accordingly, this simple method could serve as a preliminary test for GD diagnosis and therapy efficiency.

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