Effect of Solvent Composition on Solubility and Activity of Bovine Leukemia Virus Proteinase

1996 ◽  
Vol 61 (8) ◽  
pp. 1254-1260 ◽  
Author(s):  
Olga Hrušková-Heidingsfeldová ◽  
Martin Piskáček ◽  
Romana Cubínková ◽  
Aleš Zábranský ◽  
Iva Pichová
Keyword(s):  
Ethylene Glycol ◽  
Bovine Leukemia Virus ◽  
Isopropyl Alcohol ◽  
Leukemia Virus ◽  
Solvent Composition ◽  
Buffer System ◽  
Sodium Ions ◽  
Magnesium Ions ◽  
Effect Of Solvent ◽  
Low Solubility

The solubility and activity of bovine leukemia virus proteinase (BLV PR) has been examined as a function of solvent composition in order to find a buffer system which would enable to prepare samples concentrated sufficiently for crystallization attempts and other studies. Low solubility and frequent precipitation was one of the major difficulties in the course of experiments carried out with BLV PR in several laboratories. We found that buffers composed of polyvalent ions, and containing glycerol, ethylene glycol or dimethyl sulfoxide, enabled to concentrate the enzyme up to 2 mg/ml. Presence of 4-20% of DMSO, isopropyl alcohol or ethylene glycol results in activation of BLV PR, while glycerol does not significantly affect the BLV PR activity. Zinc or magnesium ions substituting for sodium ions in commonly used buffers caused decrease both in solubility and activity of BLV PR.

scholarly journals STUDY OF WETTING ON THE NON-IMAGE AREA OF OFFSET PRINTING PLATES BY AN ALTERNATIVE ISO-PROPYL ALCOHOL-FREE FOUNTAIN SOLUTION

2021 ◽  
Vol 10 (2) ◽  
Author(s):  
Anh Tuan Phung ◽  
Quang Hung Nguyen ◽  
Hong Quyen Duong ◽  
Hong Ha Cao
Keyword(s):  
Ethylene Glycol ◽  
Isopropyl Alcohol ◽  
Contact Angles ◽  
Magnesium Nitrate ◽  
Buffer System ◽  
Nitrate Hexahydrate ◽  
Offset Printing ◽  
Butyl Ether ◽  
Printing Plate ◽  
Image Area

In this study, an alternative aqueous isopropyl alcohol-free fountain solution for sheet-fed offset printing is prepared comprising 5 w/w% McIlvaine's buffer system for pH control, 0.3 w/w% Polysorbate 80 as a surfactant, 1 w/w% magnesium nitrate hexahydrate as a conductivity agent and 10 w/w% ethylene glycol mono-butyl ether as an additional wetting agent. The percentages of each composition are w/w% of active and then adjusted to 100 w/w% by adding distilled water. The contact angles of the obtained fountain solution on non-image areas of three common offset printing plates: CTP plate of Kodak, CTP plate of Huaguang Graphic Co. Ltd (China), and CTP plate of Mylan Printing Media Co. Ltd (Vietnam), range from 10° to 20°.The surface tension, conductivity, pH and viscosity were found to be 30.5 mN/m, 1.45 mS/cm, 4.8, and 1.05 x 10-2 g/cm.s, respectively. In comparison to the commercial fountain solution (KF – Trade Mark (China) containing isopropyl alcohol (IPA), the obtained fountain solution shows similar wettability on the non-image area of the offset printing plate whilst having similar physico-chemical parameters. The results indicate that ethylene glycol mono-butyl ether could be a potential substitute for IPA in fountain solution in offset printing, and the obtained fountain solution displays suitable properties for the offset printing process.

A STUDY OF HETEROGENEITY OF BOVINE LEUKEMIA VIRUS GENOTYPES IN CATTLE

2012 ◽  
pp. 69-72
Author(s):  
N.V. Bateneva ◽  
◽  
P.N. Smirnov ◽  
I.V. Mikhnovich ◽  
◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Genotypes

Bovine Leukemia Virus in Bovine Aborted Fetuses

2019 ◽  
Vol 07 (01) ◽  
Author(s):  
Kelly Cristina Santos Montanari ◽  
Marcia Mayumi Fusuma ◽  
Alessandra Maria Dias Lacerda ◽  
Líria Hiromi Okuda ◽  
Edviges Maristela Pituco ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus

A Serological Study on Bovine Leukemia Virus Infection in Ten Provinces of Iran between 2010 and 2012

2012 ◽  
Vol 6 (3) ◽  
pp. 1-7 ◽  
Author(s):  
M Kazemimanesh ◽  
O Madadgar ◽  
MR Mahzoonieh ◽  
T Zahraei-Salehi ◽  
F Steinbach ◽  
...  
Keyword(s):  
Virus Infection ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Leukemia Virus Infection ◽  
Serological Study

Fusion of bovine leukemia virus with target cells monitored by R18 fluorescence and PCR assays.

1997 ◽  
Vol 71 (1) ◽  
pp. 738-740 ◽  
Author(s):  
S Zarkik ◽  
F Defrise-Quertain ◽  
D Portetelle ◽  
A Burny ◽  
J M Ruysschaert
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Target Cells ◽  
Pcr Assays
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