Large scale syntheses of oligonucleotides on long-chain methylaminoalkyl controlled pore glass (LCMAA-CPG) supports by the NPE/NPEOC-strategy

1993 ◽  
Vol 58 (s1) ◽  
pp. 199-203
Author(s):  
Jan Weiler ◽  
Wolfgang Pfleiderer
Keyword(s):  
Large Scale ◽  
Controlled Pore Glass ◽  
Pore Glass ◽  
Long Chain

scholarly journals Rapid and Efficient Method of Immobilization of Invertase on Long Chain Akylamine Controlled Pore Glass

2017 ◽  
Vol 14 (4) ◽  
pp. 1465-1468
Author(s):  
Azmi Naqvi ◽  
Dinesh C. Sharma
Keyword(s):  
Microwave Radiation ◽  
Efficient Method ◽  
Photochemical Reaction ◽  
Functional Group ◽  
Covalent Bond ◽  
Quick Method ◽  
Controlled Pore Glass ◽  
Pore Glass ◽  
Long Chain

ABSTRACT: Herein, we describe a quick method of immobilization of invertase onto photoreactive long chain alkylamine controlled pore glass (LCAA-CPG). Photorective LCAA-CPG which is prepared using microwave radiation holds at least one photoactivable functional group capable of forming a covalent bond with the biomolecule in a photochemical reaction. Invertase is subsequently immobilized onto Photorective LCAA-CPG using photochemical reaction.

Large-Scale Synthesis of Oligodeoxyribonucleotide Phosphorothioate Using Controlled-Pore Glass as Support

1995 ◽  
Vol 14 (6) ◽  
pp. 1219-1226 ◽  
Author(s):  
Vasulinga T. Ravikumar ◽  
Mark Andrade ◽  
Tadeusz K. Wyrzykiewicz ◽  
Anthony Scozzari ◽  
Douglas L. Cole
Keyword(s):  
Large Scale ◽  
Controlled Pore Glass ◽  
Pore Glass ◽  
Large Scale Synthesis

Calorimetric Sensor for Ethanol Using Ni2+-nitrilotriacetic Acid (NTA) Resin Immobilized Alcohol Dehydrogenase (ADH)

2020 ◽  
Vol 16 (6) ◽  
pp. 795-799
Author(s):  
YongJin Li
Keyword(s):  
Cost Effective ◽  
Nitrilotriacetic Acid ◽  
Repeated Measurements ◽  
Great Promise ◽  
Calorimetric Sensor ◽  
Controlled Pore Glass ◽  
Pore Glass ◽  
One Step ◽  
Immobilization Matrix ◽  
Enzyme Thermistor

Background: A simple, fast and economic analytical method for the determination of ethanol is important for clinical, biological, forensic and physico-legal purposes. Methods: Ni2+-NTA resin was used as an immobilization matrix for the simple one-step purification/ immobilization of his6-tagged ADH. Different alcohols with a concentration range of 0.5-50% V/V, namely methanol, ethanol and propanol were measured using prepared ADH enzyme thermistor. The ethanol content of Tsingtao beer was tested as a real sample containing alcohol. Reproducibility and stability of prepared ADH enzyme thermistor were also investigated by repeated measurements. Results: In comparison to the controlled pore glass (a common used support for the immobilization of enzyme) used in thermal biosensor, the use of Ni2+-NTA resin not only led to simple one-step purification/ immobilization by his6-tagged ADH binding to Ni2+-NTA resin, but also made the immobilizing supports reusable. The prepared biosensor can be used to determine ethanol and methanol by the calorimetric measurement. A linear range of 1 -32% (V/V) and 2-20% (V/V) was observed for ethanol and methanol, respectively. The detection limits were 0.3% (V/V) and 1% (V/V) for ethanol and methanol, respectively. The tested ethanol concentration of Tsingtao beer was 4.5% V/V, which is comparable with the labeled alcohol by volume (ABV) 4.80%. Conclusion: Ni2+-NTA resin, as an immobilization matrix in ET sensor, provides a simple one-step purification/immobilization for His6-tagged recombinase and a reusable immobilization matrix. The prepared biosensor exhibits good repeatability and stability. Such a new biosensor shows great promise for rapid, simple, and cost-effective analysis of ethanol and methanol, both in qualitative and in quantitative tests.

Improved Conditions for Solid Phase Synthesis of Oligonucleotides on PS-PEG Copolymers

1995 ◽  
Vol 50 (7) ◽  
pp. 1096-1100 ◽  
Author(s):  
Ernst Bayer ◽  
Konrad Bleicher ◽  
Martin Maier
Keyword(s):  
Electrospray Mass Spectrometry ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Chemical Properties ◽  
Oligonucleotide Synthesis ◽  
Phase Synthesis ◽  
Controlled Pore Glass ◽  
Product Identification ◽  
Pore Glass ◽  
And Chemical Properties

Polystyrene-polyethylene glycol (PS-PEG) tentacle polymers with loadings of up to 60/<μmol/g were used for standard oligonucleotide synthesis. As these resins are easy to handle and stable under reaction and cleavage conditions they may be used alternatively to controlled pore glass (CPG) as the most commonly used solid support for oligonucleotide synthesis. However, structural and chemical properties of the PS-PEG resins require modified conditions to guarantee syntheses with high coupling efficiencies. Oligonucleotides (ODN ) of various sequences and lengths have successfully been synthesized using HPLC and capillary electrophoresis (CE) for purity control. Additionally, electrospray mass spectrometry (ES-MS) was used for product identification.

The occurrence of long chain polyprenols in leaves of plants of Rosaceae family and their isolation by time-extended liquid chromatography

1992 ◽  
Vol 70 (6) ◽  
pp. 448-454 ◽  
Author(s):  
Ewa Świeżewska ◽  
T. Chojnacki ◽  
W. J. Jankowski ◽  
K. Singh ◽  
J. Olsson
Keyword(s):  
Liquid Chromatography ◽  
Fatty Acid ◽  
Large Scale ◽  
Fatty Acid Esters ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Wet Weight ◽  
Rosaceae Family ◽  
Acid Esters ◽  
Long Chain

The long chain polyprenols composed of 30 and more isoprene units from leaves of plants belonging to the genera Potentilla and Rosa have been described. They occur in the form of fatty acid esters. The composition of polyprenol mixture was species dependent and its content reached ca. 0.5% wet weight. Large scale preparation of individual polyprenols from a natural polyprenol mixture was performed using time-extended liquid chromatography on the hydrophobic gel Lipidex-5000.Key words: long chain polyprenols, Rosaceae.

Dansylated Aminopropyl Controlled Pore Glass:  A Model for Silica−Liquid Solvation+

Langmuir ◽  
2004 ◽  
Vol 20 (24) ◽  
pp. 10507-10516 ◽  
Author(s):  
Phillip M. Page ◽  
Chase A. Munson ◽  
Frank V. Bright
Keyword(s):  
Controlled Pore Glass ◽  
Pore Glass

Use of controlled pore glass in solid phase oligosaccharide synthesis. Application to the semiautomated synthesis of a glyconucleotide conjugate

1998 ◽  
Vol 39 (14) ◽  
pp. 1953-1956 ◽  
Author(s):  
Matteo Adinolfi ◽  
Gaspare Barone ◽  
Lorenzo De Napoli ◽  
Alfonso Iadonisi ◽  
Gennaro Piccialli
Keyword(s):  
Solid Phase ◽  
Oligosaccharide Synthesis ◽  
Controlled Pore Glass ◽  
Pore Glass
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