Dansylated Aminopropyl Controlled Pore Glass:  A Model for Silica−Liquid Solvation+

Langmuir ◽  
2004 ◽  
Vol 20 (24) ◽  
pp. 10507-10516 ◽  
Author(s):  
Phillip M. Page ◽  
Chase A. Munson ◽  
Frank V. Bright
Keyword(s):  
Controlled Pore Glass ◽  
Pore Glass

Calorimetric Sensor for Ethanol Using Ni2+-nitrilotriacetic Acid (NTA) Resin Immobilized Alcohol Dehydrogenase (ADH)

2020 ◽  
Vol 16 (6) ◽  
pp. 795-799
Author(s):  
YongJin Li
Keyword(s):  
Cost Effective ◽  
Nitrilotriacetic Acid ◽  
Repeated Measurements ◽  
Great Promise ◽  
Calorimetric Sensor ◽  
Controlled Pore Glass ◽  
Pore Glass ◽  
One Step ◽  
Immobilization Matrix ◽  
Enzyme Thermistor

Background: A simple, fast and economic analytical method for the determination of ethanol is important for clinical, biological, forensic and physico-legal purposes. Methods: Ni2+-NTA resin was used as an immobilization matrix for the simple one-step purification/ immobilization of his6-tagged ADH. Different alcohols with a concentration range of 0.5-50% V/V, namely methanol, ethanol and propanol were measured using prepared ADH enzyme thermistor. The ethanol content of Tsingtao beer was tested as a real sample containing alcohol. Reproducibility and stability of prepared ADH enzyme thermistor were also investigated by repeated measurements. Results: In comparison to the controlled pore glass (a common used support for the immobilization of enzyme) used in thermal biosensor, the use of Ni2+-NTA resin not only led to simple one-step purification/ immobilization by his6-tagged ADH binding to Ni2+-NTA resin, but also made the immobilizing supports reusable. The prepared biosensor can be used to determine ethanol and methanol by the calorimetric measurement. A linear range of 1 -32% (V/V) and 2-20% (V/V) was observed for ethanol and methanol, respectively. The detection limits were 0.3% (V/V) and 1% (V/V) for ethanol and methanol, respectively. The tested ethanol concentration of Tsingtao beer was 4.5% V/V, which is comparable with the labeled alcohol by volume (ABV) 4.80%. Conclusion: Ni2+-NTA resin, as an immobilization matrix in ET sensor, provides a simple one-step purification/immobilization for His6-tagged recombinase and a reusable immobilization matrix. The prepared biosensor exhibits good repeatability and stability. Such a new biosensor shows great promise for rapid, simple, and cost-effective analysis of ethanol and methanol, both in qualitative and in quantitative tests.

Improved Conditions for Solid Phase Synthesis of Oligonucleotides on PS-PEG Copolymers

1995 ◽  
Vol 50 (7) ◽  
pp. 1096-1100 ◽  
Author(s):  
Ernst Bayer ◽  
Konrad Bleicher ◽  
Martin Maier
Keyword(s):  
Electrospray Mass Spectrometry ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Chemical Properties ◽  
Oligonucleotide Synthesis ◽  
Phase Synthesis ◽  
Controlled Pore Glass ◽  
Product Identification ◽  
Pore Glass ◽  
And Chemical Properties

Polystyrene-polyethylene glycol (PS-PEG) tentacle polymers with loadings of up to 60/<μmol/g were used for standard oligonucleotide synthesis. As these resins are easy to handle and stable under reaction and cleavage conditions they may be used alternatively to controlled pore glass (CPG) as the most commonly used solid support for oligonucleotide synthesis. However, structural and chemical properties of the PS-PEG resins require modified conditions to guarantee syntheses with high coupling efficiencies. Oligonucleotides (ODN ) of various sequences and lengths have successfully been synthesized using HPLC and capillary electrophoresis (CE) for purity control. Additionally, electrospray mass spectrometry (ES-MS) was used for product identification.

Use of controlled pore glass in solid phase oligosaccharide synthesis. Application to the semiautomated synthesis of a glyconucleotide conjugate

1998 ◽  
Vol 39 (14) ◽  
pp. 1953-1956 ◽  
Author(s):  
Matteo Adinolfi ◽  
Gaspare Barone ◽  
Lorenzo De Napoli ◽  
Alfonso Iadonisi ◽  
Gennaro Piccialli
Keyword(s):  
Solid Phase ◽  
Oligosaccharide Synthesis ◽  
Controlled Pore Glass ◽  
Pore Glass

Immobilization of Bovine Trypsin onto Controlled Pore Glass

2013 ◽  
Vol 38 (2) ◽  
pp. 184-195 ◽  
Author(s):  
Dan Li ◽  
Nana Akyaa Ackaah-Gyasi ◽  
Benjamin K. Simpson
Keyword(s):  
Controlled Pore Glass ◽  
Bovine Trypsin ◽  
Pore Glass

scholarly journals Hydrogen Bonding of Water Confined in Controlled-Pore Glass 10-75 Studied by1H-Solid State NMR

2007 ◽  
Vol 221 (1) ◽  
pp. 155-168 ◽  
Author(s):  
A. Vyalikh ◽  
Th. Emmler ◽  
B. Grünberg ◽  
Y. Xu ◽  
I. Shenderovich ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Solid State ◽  
Solid State Nmr ◽  
Controlled Pore Glass ◽  
Pore Glass

New noncompetitive immunoassays of small analytes

1995 ◽  
Vol 41 (7) ◽  
pp. 986-990 ◽  
Author(s):  
U Piran ◽  
W J Riordan ◽  
L A Livshin
Keyword(s):  
Detection Limit ◽  
Liquid Phase ◽  
Binding Sites ◽  
Dynamic Range ◽  
Lower Detection Limit ◽  
Controlled Pore Glass ◽  
Lower Detection ◽  
Pore Glass ◽  
Clinically Significant ◽  
Acridinium Ester

Abstract We developed a novel noncompetitive immunoassay format for monoepitopic analytes and describe here a model assay for triiodothyronine (T3), performed on Ciba Corning's ACS:180 analyzer. Acridinium ester (AE)-labeled bivalent anti-T3 was incubated with the sample, producing AE-anti-T3/T3 complexes and unreacted AE-anti-T3. Controlled-pore glass particles (CPG) with immobilized diiodothyronine (T2) were then added in excess, to bind AE-anti-T3 possessing two unoccupied binding sites but not AE-anti-T3 bound to one or two T3 molecules. Paramagnetic particles (PMP) with immobilized anti-AE were then added to the same cuvette to capture AE-anti-T3/T3 complexes; AE-anti-T3 bound to the surface of CPG, however, was not captured, because of steric hindrance. After the incubation, the PMP was magnetically separated to remove the liquid phase and the suspended CPG from the cuvette. The chemiluminescence associate with the PMP remaining in the cuvette was then measured. This noncompetitive T3 assay exhibited a 10-fold lower detection limit than the equivalent competitive T3 assay, i.e., 0.3 vs pg/test. Imprecision (CV) in the clinically significant range was 6% or less. The assay also displayed two- to sevenfold lower cross-reactivities and a wider dynamic range.

Thermal Behavior of Methyl p-hydroxybenzoate in Controlled-Pore Glass Solid Dispersion

1995 ◽  
Vol 173 (1) ◽  
pp. 186-191 ◽  
Author(s):  
Etsuo Yonemochi ◽  
Masazumi Kojima ◽  
Akiko Nakatsuji ◽  
Siriporn Okonogi ◽  
Toshio Oguchi ◽  
...  
Keyword(s):  
Thermal Behavior ◽  
Solid Dispersion ◽  
Controlled Pore Glass ◽  
Pore Glass
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