Purification of Glycerol Kinase by "Dye-Ligand" Chromatography and Hydrophobic Interaction Chromatography on Bead-Cellulose Derivatives

1993 ◽  
Vol 58 (2) ◽  
pp. 445-451 ◽  
Author(s):  
Vladimír Žúbor ◽  
Albert Breier ◽  
Marta Horváthová ◽  
Dagmar Hagarová ◽  
Peter Gemeiner ◽  
...  
Keyword(s):  
Hydrophobic Interaction ◽  
Specific Activity ◽  
Hydrophobic Interaction Chromatography ◽  
Glycerol Kinase ◽  
Enzymic Activity ◽  
Cellulose Derivatives ◽  
Long Term Storage ◽  
Bead Cellulose ◽  
Term Storage

The crude extract of cytosole enzymes was obtained from homogenized cells of Saccharomyces cerevisiae by partition. The enzyme was then isolated from the lower aqueous phase displaying higher glycerol kinase activity by dye-ligand chromatography on Cibacron Blue (CB) or Remazol Brilliant Blue R (RB)-derivatized bead-cellulose, ATP being the eluent. The specific activity of glycerol kinase rised more than 10 and 7-times after affinity dye-ligand chromatography and hydrophobic interaction chromatography, respectively. Glycerol kinase obtained by the latter method was purified by CB-bead cellulose. The final preparation maintained its enzymic activity without noticeable losses during a long-term storage at 4 °C in dark.

Derivatives of cyclosporin compatible with antibody-based assays: I. The generation of [125I]-labeled cyclosporin.

1985 ◽  
Vol 31 (3) ◽  
pp. 459-462 ◽  
Author(s):  
W C Mahoney ◽  
J W Orf
Keyword(s):  
Cyclosporin A ◽  
Specific Activity ◽  
Immunosuppressive Drug ◽  
Clinical Samples ◽  
Long Term Storage ◽  
Derivatives Of ◽  
Term Storage

Abstract The immunosuppressive drug cyclosporin A, has been successfully iodinated to a specific activity of 300 Ci per gram. 125I-labeled cyclosporin and [3H]cyclosporin are nearly equivalent as tracers in a radioimmunoassay in producing standard lines (suppression by unlabeled cyclosporin) and in assigning values to clinical samples. In addition, the [125I]-labeled cyclosporin has greater than twice the sensitivity, and it is stable to long-term storage. Use of a [125I]-labeled cyclosporin tracer is more convenient, more reproducible, more precise, and easier than the tritiated-cyclosporin alternative in radioimmunoassay of this compound.

scholarly journals A novel one-step purification of human α-thrombin after direct activation of crude prothrombin enriched from plasma

1991 ◽  
Vol 280 (3) ◽  
pp. 805-808 ◽  
Author(s):  
P K Ngai ◽  
J Y Chang
Keyword(s):  
Specific Activity ◽  
Single Step ◽  
National Institutes Of Health ◽  
Long Term Storage ◽  
Freeze Dried ◽  
Specific Affinity ◽  
One Step ◽  
Term Storage ◽  
Laser Densitometry

Crude prothrombin enriched from human plasma was directly activated to generate alpha-thrombin without prior exhaustive purification of the proenzyme using a combination of several different types of chromatographic techniques, as in all previously described methodologies. Activated thrombin was separated from other components in a single step by taking advantage of its highly specific affinity to heparin immobilized on a matrix support of Sepharose CL-6B. On the basis of the data presented herein, we have demonstrated the ease with which at least 25 mg of a highly purified enzyme (greater than 97% homogeneous by laser densitometry) can be obtained per litre of plasma. Our product exhibits a specific activity of at least 4000 National Institutes of Health units/mg and is stable after being freeze-dried for the purpose of long-term storage.

scholarly journals A comprehensively revised strategy that improves the specific activity and long-term stability of clinically relevant89Zr-immuno-PET agents

2018 ◽  
Vol 47 (37) ◽  
pp. 13214-13221 ◽  
Author(s):  
Nikunj B. Bhatt ◽  
Darpan N. Pandya ◽  
Stephanie Rideout-Danner ◽  
Howard D. Gage ◽  
Frank C. Marini ◽  
...  
Keyword(s):  
Specific Activity ◽  
Production Quality ◽  
Emission Tomography ◽  
Production Quality Control ◽  
Long Term Stability ◽  
Long Term Storage ◽  
Positron Emission ◽  
Pet Radiopharmaceuticals ◽  
Term Storage

Zirconium-89 is currently being used in numerous clinical trials involving monoclonal antibodies and positron emission tomography. A comprehensively revised protocol for the production, quality control and long-term storage of89Zr-immuno-PET radiopharmaceuticals.

Highly efficient long-term storage of carrier-bound anammox biomass

2016 ◽  
Vol 74 (8) ◽  
pp. 1911-1918
Author(s):  
Steffen Engelbrecht ◽  
Mbengamina Terence Fondengcap ◽  
Kristina Rathsack ◽  
Marion Martienssen
Keyword(s):  
Specific Activity ◽  
Anammox Bacteria ◽  
Simple Method ◽  
Long Term Storage ◽  
Generation Times ◽  
Anammox Activity ◽  
Potential Alternative ◽  
Redox Buffer ◽  
Term Storage

The anammox process is a potential alternative to the conventional nitrogen removal from wastewater. However, due to large generation times of anammox bacteria, the start-up of treatment reactors may be impeded. An efficient storage technique can handle this drawback and may be also suitable for seasonally operated treatment plants like in touristic areas. In the current study, several storage techniques were investigated with respect to its suitability for the preservation of the specific anammox activity after long-term storage. Storing conditions differed in terms of temperature, redox buffer and nutrient supplementation. The specific activity of immobilized anammox bacteria (Candidatus Kuenenia stuttgartiensis) was determined three times during a long-term preservation of 78 days and 106 days, respectively. The highest activity was ensured at a storing temperature of 4 °C, providing nitrate as redox buffer and a nutrient supplement every 23 days. Thus, 91.4% of the initial anammox activity could be preserved after a storage of 106 days. Superiority of the presented treatment condition was confirmed by a calculated nitrate-ammonium consumption rate close to the optimal ratio of 1.32. This technique provided an economical and simple method suitable for long-term storage of immobilized anammox biomass.

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Long Term Storage ◽  
Term Storage
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