scholarly journals A comprehensively revised strategy that improves the specific activity and long-term stability of clinically relevant89Zr-immuno-PET agents

2018 ◽  
Vol 47 (37) ◽  
pp. 13214-13221 ◽  
Author(s):  
Nikunj B. Bhatt ◽  
Darpan N. Pandya ◽  
Stephanie Rideout-Danner ◽  
Howard D. Gage ◽  
Frank C. Marini ◽  
...  
Keyword(s):  
Specific Activity ◽  
Production Quality ◽  
Emission Tomography ◽  
Production Quality Control ◽  
Long Term Stability ◽  
Long Term Storage ◽  
Positron Emission ◽  
Pet Radiopharmaceuticals ◽  
Term Storage

Zirconium-89 is currently being used in numerous clinical trials involving monoclonal antibodies and positron emission tomography. A comprehensively revised protocol for the production, quality control and long-term storage of89Zr-immuno-PET radiopharmaceuticals.

Purification of Glycerol Kinase by "Dye-Ligand" Chromatography and Hydrophobic Interaction Chromatography on Bead-Cellulose Derivatives

1993 ◽  
Vol 58 (2) ◽  
pp. 445-451 ◽  
Author(s):  
Vladimír Žúbor ◽  
Albert Breier ◽  
Marta Horváthová ◽  
Dagmar Hagarová ◽  
Peter Gemeiner ◽  
...  
Keyword(s):  
Hydrophobic Interaction ◽  
Specific Activity ◽  
Hydrophobic Interaction Chromatography ◽  
Glycerol Kinase ◽  
Enzymic Activity ◽  
Cellulose Derivatives ◽  
Long Term Storage ◽  
Bead Cellulose ◽  
Term Storage

The crude extract of cytosole enzymes was obtained from homogenized cells of Saccharomyces cerevisiae by partition. The enzyme was then isolated from the lower aqueous phase displaying higher glycerol kinase activity by dye-ligand chromatography on Cibacron Blue (CB) or Remazol Brilliant Blue R (RB)-derivatized bead-cellulose, ATP being the eluent. The specific activity of glycerol kinase rised more than 10 and 7-times after affinity dye-ligand chromatography and hydrophobic interaction chromatography, respectively. Glycerol kinase obtained by the latter method was purified by CB-bead cellulose. The final preparation maintained its enzymic activity without noticeable losses during a long-term storage at 4 °C in dark.

scholarly journals Long-Term Stability of the Electronic Sensor Component of a Digital Pill System in Real-World Storage Settings

2021 ◽  
pp. 875512252098521
Author(s):  
Peter R. Chai ◽  
Georgia Goodman ◽  
Majo J. Bustamante ◽  
Yassir Mohamed ◽  
Jose Castillo-Mancilla ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Gastric Fluid ◽  
Simulated Gastric Fluid ◽  
Once Daily ◽  
Long Term Stability ◽  
Long Term Storage ◽  
System Requirements ◽  
The Stability ◽  
Term Storage

Background: Digital pill systems comprise an ingestible sensor integrated into a gelatin capsule that overencapsulates medication allowing real-time measures of medication ingestion. These systems may improve the manner in which medication adherence can be assessed and supported. Objective: In this investigation, we tested the durability of the ingestible sensor as part of a clinical trial to measure the feasibility and acceptability of the system to measure adherence to once daily tenofovir disoproxil fumarate/emtricitabine (NCT03842436). Methods: Digital pills not dispensed during the study were stored in a pharmacy. Seventeen sensors were selected from digital pills stored for at least 12 months and activated in a simulated gastric environment. A radiofrequency spectrum analyzer and the reader device used in the clinical trial to capture ingestion events were used to measure activation of emitters. A passing evaluation was defined as an energized emitter within 30 minutes of immersion, ability to broadcast a signal for 10 minutes, and successful acquisition by the reader. Results: All ingestible sensors passed the stability test. Mean activation time in simulated gastric fluid was 3.33 minutes (SD = 1.47); emitters remained active for a mean of 47.72 minutes (SD = 1.78). These parameters matched guidelines defined in the ID-Cap system requirements for use in patients. Conclusions: Ingestible sensor components of the ID-Cap system were therefore stable after long-term storage.

Long-term stability of water samples— reanalysis of 20-year-old unpreserved samples

2019 ◽  
Vol 193 (2) ◽  
pp. 167-171
Author(s):  
Espen Enge
Keyword(s):  
Ambient Temperature ◽  
Water Samples ◽  
Water Bodies ◽  
Total Hardness ◽  
Term Stability ◽  
Long Term Stability ◽  
Long Term Storage ◽  
Stability Of Water ◽  
Term Storage

The current study compiles data from the reanalysis of 25 water samples stored unpreserved at ambient temperature for 20 years. Small changes in pH, conductivity and chloride and no significant effects on Al and total hardness were detected. The small effects measured, even during long-term storage under unsuitable conditions, suggest that samples taken from pristine water bodies remain chemically stable over many years.

Derivatives of cyclosporin compatible with antibody-based assays: I. The generation of [125I]-labeled cyclosporin.

1985 ◽  
Vol 31 (3) ◽  
pp. 459-462 ◽  
Author(s):  
W C Mahoney ◽  
J W Orf
Keyword(s):  
Cyclosporin A ◽  
Specific Activity ◽  
Immunosuppressive Drug ◽  
Clinical Samples ◽  
Long Term Storage ◽  
Derivatives Of ◽  
Term Storage

Abstract The immunosuppressive drug cyclosporin A, has been successfully iodinated to a specific activity of 300 Ci per gram. 125I-labeled cyclosporin and [3H]cyclosporin are nearly equivalent as tracers in a radioimmunoassay in producing standard lines (suppression by unlabeled cyclosporin) and in assigning values to clinical samples. In addition, the [125I]-labeled cyclosporin has greater than twice the sensitivity, and it is stable to long-term storage. Use of a [125I]-labeled cyclosporin tracer is more convenient, more reproducible, more precise, and easier than the tritiated-cyclosporin alternative in radioimmunoassay of this compound.

scholarly journals Instability of corticotropin during long-term storage – myth or reality?

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Jacquelien J. Hillebrand ◽  
Li Zhou ◽  
Marilee A. Marcinkus ◽  
Maria Datwyler ◽  
Susan H. Gawel ◽  
...  
Keyword(s):  
Storage Time ◽  
Storage Temperature ◽  
Ethylenediaminetetraacetic Acid ◽  
Venous Blood ◽  
Sampling Studies ◽  
Term Stability ◽  
Long Term Stability ◽  
Long Term Storage ◽  
Term Storage

Abstract Objectives Corticotropin is notorious for its instability. Whereas several studies have investigated its short-term stability in plasma following venous blood sampling, studies on long-term stability are lacking. Here we investigated the long-term storage stability of corticotropin in ethylenediaminetetraacetic acid containing plasma. Methods Specimens from healthy volunteers (neat, spiked) were stored in polypropylene microcentrifuge tubes with socket screw-caps at −20 °C and −70 °C for up to one and a half years. Corticotropin in plasma was measured using an Abbott research only immunoassay. Separately, specimens from patients were collected during diagnostic routine testing and stored in polystyrene tubes with push-caps at −20 °C for up to 6 years. In these samples corticotropin hormone was measured using the Diasorin corticotropin immunoassay. Results Storage of specimens at −20 °C or −70 °C for up to one and a half years showed minimal changes (<11%) in corticotropin levels, while storage of patient samples at −20 °C for up to 6 years showed a significant (54%) reduction in corticotropin levels. Conclusions Corticotropin levels are stable in plasma when stored at −20 °C for one and a half years using the Abbott research only assay, but with longer storage time a significant reduction in corticotropin levels can be expected. Once specimens are stored for future corticotropin measurements, one should consider storage time, storage temperature and assay differences.

Suitability of Flash Media for the Long-Term Storage of Information

2019 ◽  
Vol 40 (2) ◽  
pp. 97-122
Author(s):  
Joe Iraci
Keyword(s):  
High Capacity ◽  
Accelerated Ageing ◽  
Flash Storage ◽  
Long Term Stability ◽  
Long Term Storage ◽  
Storage Media ◽  
The Stability ◽  
To Receive ◽  
Term Storage

Abstract Flash storage media such as memory cards and USB flash drives are now commonly used to transfer and store information. However, little is known about the long-term stability of this type of media and this is a concern for archives and other institutions as they begin to receive content stored on these devices. In this study, the stabilities of a variety of different flash media were examined. The evaluation was performed by using accelerated ageing at 85 °C and 85 % relative humidity (RH) and 125 °C for ageing intervals up to 2000 hours. Measurements were also performed on samples previously subjected to accelerated ageing and then naturally aged for five years to verify the results from the accelerated ageing experiments. Overall, the stability of flash media was very good. For many of the samples, no read errors were encountered after accelerated or natural ageing. However, for several of the high capacity flash card samples and USB flash drives, significant decreases in read speed were noted. This can be problematic because it will eventually lead to read errors. It was established that for the USB samples this instability was likely attributed to the use of the less stable TLC (triple-level cell) memory chip.

scholarly journals A novel one-step purification of human α-thrombin after direct activation of crude prothrombin enriched from plasma

1991 ◽  
Vol 280 (3) ◽  
pp. 805-808 ◽  
Author(s):  
P K Ngai ◽  
J Y Chang
Keyword(s):  
Specific Activity ◽  
Single Step ◽  
National Institutes Of Health ◽  
Long Term Storage ◽  
Freeze Dried ◽  
Specific Affinity ◽  
One Step ◽  
Term Storage ◽  
Laser Densitometry

Crude prothrombin enriched from human plasma was directly activated to generate alpha-thrombin without prior exhaustive purification of the proenzyme using a combination of several different types of chromatographic techniques, as in all previously described methodologies. Activated thrombin was separated from other components in a single step by taking advantage of its highly specific affinity to heparin immobilized on a matrix support of Sepharose CL-6B. On the basis of the data presented herein, we have demonstrated the ease with which at least 25 mg of a highly purified enzyme (greater than 97% homogeneous by laser densitometry) can be obtained per litre of plasma. Our product exhibits a specific activity of at least 4000 National Institutes of Health units/mg and is stable after being freeze-dried for the purpose of long-term storage.

Highly efficient long-term storage of carrier-bound anammox biomass

2016 ◽  
Vol 74 (8) ◽  
pp. 1911-1918
Author(s):  
Steffen Engelbrecht ◽  
Mbengamina Terence Fondengcap ◽  
Kristina Rathsack ◽  
Marion Martienssen
Keyword(s):  
Specific Activity ◽  
Anammox Bacteria ◽  
Simple Method ◽  
Long Term Storage ◽  
Generation Times ◽  
Anammox Activity ◽  
Potential Alternative ◽  
Redox Buffer ◽  
Term Storage

The anammox process is a potential alternative to the conventional nitrogen removal from wastewater. However, due to large generation times of anammox bacteria, the start-up of treatment reactors may be impeded. An efficient storage technique can handle this drawback and may be also suitable for seasonally operated treatment plants like in touristic areas. In the current study, several storage techniques were investigated with respect to its suitability for the preservation of the specific anammox activity after long-term storage. Storing conditions differed in terms of temperature, redox buffer and nutrient supplementation. The specific activity of immobilized anammox bacteria (Candidatus Kuenenia stuttgartiensis) was determined three times during a long-term preservation of 78 days and 106 days, respectively. The highest activity was ensured at a storing temperature of 4 °C, providing nitrate as redox buffer and a nutrient supplement every 23 days. Thus, 91.4% of the initial anammox activity could be preserved after a storage of 106 days. Superiority of the presented treatment condition was confirmed by a calculated nitrate-ammonium consumption rate close to the optimal ratio of 1.32. This technique provided an economical and simple method suitable for long-term storage of immobilized anammox biomass.

scholarly journals Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Pavlina Holcapkova ◽  
Zuzana Kolarova Raskova ◽  
Martina Hrabalikova ◽  
Alexandra Salakova ◽  
Jan Drbohlav ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Polyethylene Glycol ◽  
High Performance ◽  
Thermal Stabilization ◽  
Nisin Production ◽  
Long Term Stability ◽  
Long Term Storage ◽  
The Stability ◽  
Term Storage

This work describes novel alternative for extraction of bacteriocin nisin from a whey fermentation media and its stabilization by using polyethylene glycol as matrix with high practical applicability. This product was compared with commercially available nisin product stabilized by sodium chloride and nisin extracted and stabilized by using ammonium sulfate and polysorbate 80. The stability of samples was tested by means of long-term storage at −18, 4, 25, and 55°C up to 165 days. The nisin content in the samples was determined by high-performance liquid chromatography and electrophoresis. In addition, effect of whey fortification with lactose on nisin production and antibacterial activity studied against Staphylococcus aureus was tested. Results show that stabilization by polyethylene glycol provides enhanced nisin activity at 55°C after 14 days and long-term stability at 25°C with keeping antibacterial activity.

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