Antrachinon-triazine derivatives of polysaccharides, affinity of bovine heart lactate dehydrogenase to Cibacron Blue derivatives of water-soluble dextran fractions and hydroxyethyl-starches

1984 ◽  
Vol 49 (2) ◽  
pp. 549-554 ◽  
Author(s):  
Jana Barthová ◽  
Marie Tichá ◽  
Peter Gemeiner ◽  
Danica Mislovičová
Keyword(s):  
Lactate Dehydrogenase ◽  
Molecular Mass ◽  
Water Soluble ◽  
Relative Molecular Mass ◽  
Cibacron Blue ◽  
Strength Of Interaction ◽  
Triazine Derivatives ◽  
Starch Derivatives ◽  
Derivatives Of ◽  
Hydroxyethyl Starches

The interaction of lactate dehydrogenase with high-molecular-weight derivatives of Cibacron Blue was followed by measuring the reaction kinetics and by means of affinity electrophoresis. The dye was covalently bound to dextrans with relative molecular mass ranging from 5 000 to 2 000 000 and to hydroxyethyl-starches with relative molecular mass from 130 000 to 2 000 000. In the case of dextran derivatives of Cibacron Blue, various degrees of substitution by the dye were tested. Measurement of kinetics showed that the Cibacron Blue derivatives competed with NADH for the binding site on the enzyme and that when the dye was bound to a polysaccharide, the affinity of lactate dehydrogenase to the dye decreased. Both methods used confirmed that the strength of interaction did not depend on the relative molecular mass of the polysaccharide carrier or on the degree of substitution. The interaction of lactate dehydrogenase with hydroxyethyl-starch derivatives of Cibacron Blue was weaker than with dextran derivatives.

Inhibition of lactate dehydrogenase ex rabbit muscle by Cibacron Blue 3G-A bound to water-soluble hydroxyethylcellulose

1985 ◽  
Vol 50 (6) ◽  
pp. 1335-1340 ◽  
Author(s):  
Danica Mislovičová ◽  
Peter Gemeiner ◽  
Ladislav Šoltés
Keyword(s):  
Lactate Dehydrogenase ◽  
Molecular Mass ◽  
Covalent Binding ◽  
Polymeric Matrix ◽  
Water Soluble ◽  
Rabbit Muscle ◽  
Cibacron Blue ◽  
Molecular Masses

The interaction between lactate dehydrogenase and hydroxyethylcellulose - Cibacron Blue 3G-A conjugates was investigated kinetically. These conjugates were obtained by covalent binding of Cibacron Blue to water-soluble hydroxyethylcelluloses, of average relative molecular masses 2.5 . 104 to 11.6 . 104. The polymeric matrix (non-branched β-glucan) was, unlike the branched α-glucans, found to inhibit the action of the dye, irrespective of the molecular mass of this matrix. With increasing amount of the bound dye the interaction between the dye and the enzyme was enhanced.

Antraquinone-triazine derivatives of polysaccharides. Relation between structure and affinity to lactate dehydrogenase

1981 ◽  
Vol 46 (2) ◽  
pp. 419-427 ◽  
Author(s):  
Peter Gemeiner ◽  
Danica Mislovičová ◽  
Jiří Zemek ◽  
Ľudovít Kuniak
Keyword(s):  
Lactate Dehydrogenase ◽  
Degree Of Substitution ◽  
Brilliant Blue ◽  
Rabbit Muscle ◽  
Muscle Lactate ◽  
Cibacron Blue ◽  
Triazine Derivatives ◽  
Covalently Linked ◽  
The Relationship ◽  
Derivatives Of

The inhibition effects (I50) of a series of dyes (Cibacrom Blue 3G-A, Procion Blue MX-R, Remazo Brilliant Blue and Ostazin Brilliant Red S5-B) covalently linked to dextran T 70 were the criterion of the relationship between the structure of antraquinone-triazine compounds and their affinity to rabbit muscle lactate dehydrogenase (LDH). The I50 values indicate a substantial importance of the terminal benzenesulfonane moiety in the structure of Cibacron Blue. Also the degree of substitution of polysaccharide plays an important role in the affinity; its influence upon I50 can be expressed for Cibacron Blue-Dextrans T 70 by an equation of line. The influence of the structure of the polysaccharide constituent on the affinity of Cibacron Blue-(α-glucans) towards LDH is discussed, as well.

A case of immunoglobulin G conjugated with lactate dehydrogenase, producing both loss of enzyme activity and an abnormal isoenzyme pattern.

1986 ◽  
Vol 32 (7) ◽  
pp. 1420-1422 ◽  
Author(s):  
K Sudo ◽  
M Maekawa ◽  
H Watanabe ◽  
K Matsumoto ◽  
Y Mori ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Molecular Mass ◽  
Immunoglobulin G ◽  
Myocardial Hypertrophy ◽  
Isoenzyme Pattern ◽  
Relative Molecular Mass

Abstract We present a patient with an inhibitor of lactate dehydrogenase (LD; EC 1.1.1.27) in serum, who had pericarditis and myocardial hypertrophy. The patient's LD activity in serum was low and the LD isoenzyme pattern was abnormal. The relative molecular mass of this abnormal LD-IgG complex was approximately 490 000. The patient's IgG reacted with all five LD isoenzymes.

Differentiation of 2-Alkylthioorotic Acids, Methyl and Ethyl 2-Alkylthioorotates and Hydrazides of 2-Alkylthioorotic Acids by Using Electron Ionization Mass Spectra

2008 ◽  
Vol 14 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Grazyna Bartkowiak
Keyword(s):  
Molecular Mass ◽  
Mass Spectra ◽  
Chemical Species ◽  
Molecular Ions ◽  
Electron Ionization ◽  
Relative Molecular Mass ◽  
Ionization Mass ◽  
Acid Methyl ◽  
Electron Ionization Mass Spectra ◽  
Derivatives Of

Electron ionization mass spectra of 12 derivatives of 2-thioorotic acid have been discussed and general fragmentation routes of their molecular ions have been proposed. The compounds under discussion were three groups of four chemical species (2-alkylthioorotic acid, methyl 2-alkylthioorotate, ethyl 2-alkylthioorotate and 2-alkylthioorotic hydrazide) each with the same relative molecular mass. The comparison of selected ions relative abundances and their correlation with the abundance of molecular ions enable differentiation between isomeric or isobaric species in this class of compounds.

The preparation and some reactions of symmetrical Dichloro-1,3,5-triazine derivatives of polyethylene glycols

1971 ◽  
Vol 24 (12) ◽  
pp. 2649 ◽  
Author(s):  
FW Ayscough ◽  
IN Simpson
Keyword(s):  
Polyethylene Glycol ◽  
Diethylene Glycol ◽  
Triethylene Glycol ◽  
Water Soluble ◽  
Aminobutyric Acid ◽  
Polyethylene Glycols ◽  
Triazine Derivatives ◽  
Derivatives Of

The preparation is described of the bis(dichlorotriazinyl) derivatives of diethylene and triethylene glycol and of the bis(chlorobutylaminotriazinyl), bis(chlorobutylthiotriazinyl), and bis(chlorophenoxytriazinyl) derivatives of diethylene glycol. In addition, there is described the preparation of a water-soluble bis(dichlorotriazinyl) derivative of a polyethylene glycol and evidence is produced for its reactions with glycine, γ-aminobutyric acid, N- acetylcysteine, glutathione, and tyrosine.

[Mo154(NO)14O420(OH)28(H2O)70](25± 5)−: A Water-Soluble Big Wheel with More than 700 Atoms and a Relative Molecular Mass of About 24000

1995 ◽  
Vol 34 (19) ◽  
pp. 2122-2124 ◽  
Author(s):  
Achim Müller ◽  
Erich Krickemeyer ◽  
Jochen Meyer ◽  
Hartmut Bögge ◽  
Frank Peters ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Water Soluble ◽  
Relative Molecular Mass

ChemInform Abstract: (Mo154(NO)14O420(OH)28(H2O)70)(25.+-.5)-: A Water-Soluble Giant Wheel with More than 700 Atoms and a Relative Molecular Mass of 24000.

ChemInform ◽  
2010 ◽  
Vol 27 (1) ◽  
pp. no-no
Author(s):  
A. MUELLER ◽  
E. KRICKEMEYER ◽  
J. MEYER ◽  
H. BOEGGE ◽  
F. PETERS ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Water Soluble ◽  
Relative Molecular Mass

Potential anticonvulsants: Substituted N-benzylidene derivatives of 10-(4-aminopiperazino)-10,11-dihydrodibenzo[b,f]thiepins

1983 ◽  
Vol 48 (4) ◽  
pp. 1173-1186 ◽  
Author(s):  
Václav Bártl ◽  
Jiří Holubek ◽  
Emil Svátek ◽  
Marie Bartošová ◽  
Miroslav Protiva
Keyword(s):  
Acute Toxicity ◽  
Water Soluble ◽  
Hypotensive Activity ◽  
Anticonvulsant Effect ◽  
Maximal Electroshock ◽  
Soluble Salts ◽  
Sleeping Time ◽  
Central Depression ◽  
Derivatives Of ◽  
Electroshock Seizures

Reactions of 10-(4-aminopiperazino)-10,11-dihydrodibenzo[b,f]thiepins XIVa-XIVd with benzaldehyde, 3,4-dimethoxybenzaldehyde, 4-dimethylaminobenzaldehyde, salicylaldehyde, 3-ethoxy-4-hydroxybenzaldehyde, 2-(2-dimethylaminoethoxy)benzaldehyde, 3-(2-dimethylaminoethoxy)benzaldehyde and 3-ethoxy-4-(2-dimethylaminoethoxy)benzaldehyde afforded a series of 19 hydrazones IIIa-Xc. Some of them showed the expected anticonvulsant effect but only towards pentetrazole; antagonism of maximal electroshock seizures was not observed. In general, the products have a character of tranquillizers: in higher does they produce central depression, potentiate the thiopental sleeping time, have hypothermic action; in single cases antiamphetamine, antireserpine, antihistamine and cataleptic effects were observed. The water-soluble salts of the basic hydrazones VIIIa, VIIIc, IXc and Xc, administered parenterally, showed a rather high acute toxicity and revealed also adrenolytic and hypotensive activity.

Extraction of polyethylene glycols with dicarbolide solutions in nitrobenzene

1990 ◽  
Vol 55 (8) ◽  
pp. 1959-1967 ◽  
Author(s):  
Petr Vaňura ◽  
Pavel Selucký
Keyword(s):  
Polyethylene Glycol ◽  
Molecular Mass ◽  
Extraction Constant ◽  
Metal Extraction ◽  
Polyethylene Glycols ◽  
Relative Molecular Mass ◽  
Published Data ◽  
Average Molecular Mass ◽  
Peg 400 ◽  
Extraction Constants

The extraction of polyethylene glycol of average molecular mass 400 (PEG 400) with dicarbolide solution in nitrobenzene and of longer-chain polyethylene glycol, of average molecular mass 1 500 (PEG 1 500), with chlorinated dicarbolide solution in nitrobenzene was studied. During the extraction of PEG 400, the polyethylene glycol solvates the Horg+ ion in the organic phase giving rise to the HLorg+ species (L is polyethylene glycol). The obtained value of the extraction constant Kex(HLorg+) = 933 is consistent with published data of metal extraction. Extraction of PEG 1 500 was treated applying the simplified assumption that the thermodynamic behaviour of PEG 1 500 is the same as that of n molecules of polyethylene glycol with relative molecular mass 1 500/n, each solvating one cation. For this model, the value of n = 3.2 ± 1.1 and the values of the extraction constants of the HL1/n,org+ and HL2/n,org+ species were obtained by using the adapted program LETAGROP. This value of n is consistent with published extraction data in the presence of polyethylene glycol with a relative molecular mass from 200 to 1 000.

scholarly journals Antioxidant Network Based on Sulfonated Polyhydroxyalkanoate and Tannic Acid Derivative

2021 ◽  
Vol 8 (1) ◽  
pp. 9
Author(s):  
Laura Brelle ◽  
Estelle Renard ◽  
Valerie Langlois
Keyword(s):  
Gallic Acid ◽  
Tannic Acid ◽  
Water Soluble ◽  
Gel Formation ◽  
Ene Reaction ◽  
Inorganic Cations ◽  
Medium Chain Length ◽  
Physically Crosslinked ◽  
The Stability ◽  
Derivatives Of

A novel generation of gels based on medium chain length poly(3-hydroxyalkanoate)s, mcl-PHAs, were developed by using ionic interactions. First, water soluble mcl-PHAs containing sulfonate groups were obtained by thiol-ene reaction in the presence of sodium-3-mercapto-1-ethanesulfonate. Anionic PHAs were physically crosslinked by divalent inorganic cations Ca2+, Ba2+, Mg2+ or by ammonium derivatives of gallic acid GA-N(CH3)3+ or tannic acid TA-N(CH3)3+. The ammonium derivatives were designed through the chemical modification of gallic acid GA or tannic acid TA with glycidyl trimethyl ammonium chloride (GTMA). The results clearly demonstrated that the formation of the networks depends on the nature of the cations. A low viscoelastic network having an elastic around 40 Pa is formed in the presence of Ca2+. Although the gel formation is not possible in the presence of GA-N(CH3)3+, the mechanical properties increased in the presence of TA-N(CH3)3+ with an elastic modulus G’ around 4200 Pa. The PHOSO3−/TA-N(CH3)3+ gels having antioxidant activity, due to the presence of tannic acid, remained stable for at least 5 months. Thus, the stability of these novel networks based on PHA encourage their use in the development of active biomaterials.

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