Production of biomass from sulphite liquors in tower fermentor with forced circulation

1983 ◽  
Vol 48 (7) ◽  
pp. 1984-1995 ◽  
Author(s):  
Jindřich Zahradník ◽  
Mojmír Rychtera ◽  
Jan Kratochvíl ◽  
Ludmila Havlíčková ◽  
Jan Čermák
Keyword(s):  
Candida Utilis ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Favourable Effect ◽  
Active Volume ◽  
Forced Circulation ◽  
Energy Characteristics ◽  
Gas Distributor ◽  
Cultivation Process ◽  
Tower Fermentor

Cultivation of the yeast Candida utilis on sulphite liquors was studied in a tower fermentor with ejector-type gas distributor and additional bubble bed showering. The aim of the work was to test the fermentor applicability for the cultivation process studied and to obtain data characterizing the fermentation in the tested unit. The effect of medium composition and dilution rate on selected cultivation parameters was determined in the fermentor with active volume 0.1 m3 and the hydrodynamic and energy characteristics of the apparatus were evaluated. The experimental results proved the fermentor to be suitable for the cultivation process studied. Favourable effect of the optimized fermentation medium was observed both on the values of characteristic cultivation parameters and on the efficiency of the utilization of energy supplied to the system.

Application of tower bioreactors with forced circulation in biomass production

1981 ◽  
Vol 46 (13) ◽  
pp. 3232-3246 ◽  
Author(s):  
František Kaštánek ◽  
Jindřich Zahradník ◽  
Mojmír Rychtera ◽  
Jan Kratochvíl ◽  
Jan Čermák
Keyword(s):  
Fermentation Process ◽  
Candida Utilis ◽  
Continuous Cultivation ◽  
Process Conditions ◽  
Foam Formation ◽  
Internal Circulation ◽  
Forced Circulation ◽  
Gas Distributor ◽  
Liquid Dispersion ◽  
Reactor Types

Characteristic hydrodynamic parameters of three selected types of bubble-type column reactors with forced liquid circulation were studied experimentally. Application of these units for the fermentation process - production of fodder protein by continuous cultivation of Candida utilis yeast on the synthetic ethanol was tested. Two modifications of the column reactor with the ejector gas distributor and the bubble type tower reactor with the central tube and forced internal circulation of the gas-liquid dispersion were selected. Diameter of all three reactors used was 0.3 m. The volumetric mass transfer coefficient (kLa) and gas holdup (εG) were determined both for the standard water-air system and under actual fermentation process conditions. The effect of the inlet ethanol concentration and of the dilution rate on the cultivation characteristics was determined during the cultivation experiments. The obtained results have proved that the studied reactor types are suitable for fermentation. Equipment with the ejector gas distributor can be recommended even for processes with high requirements on the oxygen consumption, while by the proposed construction arrangement the undesired foam formation in the system was successfully eliminated.

Optimization of Medium Components for β-Glucosidase Production from Aspergillus Niger by Response Surface Methodology

2013 ◽  
Vol 419 ◽  
pp. 328-333 ◽  
Author(s):  
Chao Zhang ◽  
Rui Huang ◽  
Hui Tian ◽  
Ru Ming Zhao ◽  
Fa Shun Yu ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Influence Factors ◽  
Mutual Effect ◽  
Tween 80 ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Malt Extract ◽  
Medium Components

β-Glucosidase is the key enzyme for the utilization of lignocellulose.But the commercial β-glucosidase can’t be produced. This paper focuses on the study of the β-glucosidase fermentation process.The fermentation medium components for β-glucosidase production from Aspergil lusniger was optimized by response surface methodology (RSM). Firstly, the three of the most important influence factors yeast extract, MnSO4•H2O and MgSO4•7H2O was obtained from Plackett-Burman design screening. Then the path of steepest ascent experiment was adopted to approach the optimal region of the medium composition. Lastly, the optimal concentration and mutual effect of three factors were predicted by RSM. The results showed that the best medium composition was Malt extract 18g/L, Yeast extract 3.22g/L, KH2PO4 3g/L, MnSO4•H2O 0.58mM, Tween-80 0.5mL/L and MgSO4•7H2O 0.23g/L. Under these fermentation conditions, the activity of β-glucosidase was up to 7.33IU/mL with increasing 23.2% than before.

scholarly journals РАЗРАБОТКА ВЫСОКОАКТИВНОГО ШТАММА-ПРОДУЦЕНТА ВИРДЖИНИАМИЦИНА И ПОВЫШЕНИЕ ЕГО ПРОДУКТИВНОСТИ С ПОМОЩЬЮ СИНТЕТИЧЕСКИХ АБСОРБИРУЮЩИХ СМОЛ

2016 ◽  
Vol 6 (3) ◽  
pp. 195-208 ◽  
Author(s):  
V. A. Savushkin ◽  
V. V. Dzhavakhiya ◽  
E. V. Glagoleva ◽  
V. V. Savel'eva ◽  
E. D. Popova ◽  
...  
Keyword(s):  
Fermentation Medium ◽  
Culture Broth ◽  
Medium Composition ◽  
Basic Medium ◽  
High Productivity ◽  
Highly Active ◽  
Different Types ◽  
Total Productivity ◽  
Industrial Use ◽  
Selection Of

<p>Virginiamycin, an antibiotic produced by some <em>Streptomyces</em> species, is widely used in veterinary and bioethanol production. It represents a natural mix of two different macrocyclic components, among which M1 and S1 factors are the main acting components. M1 and S1 act synergistically when present in the optimum ratio of 60-75% of M1 and 25-40% of S1. Due to a large number of genes involved into the virginiamycin biosynthesis, the development of overproducing strains able to synthesize M1 and S1 at a synergistic ratio with the total productivity exceeding 3-4 g/L still remains a relevant problem. Using a multi-step random UV mutagenesis of the <em>Streptomyces</em> sp. strain DSM40559, a highly active strain S 15-30 was obtained, which virginiamycin titer on a basic medium significantly increased that of the parental strain (2.6 and 0.35 g/L, respectively), and the M1:S1 ratio remained synergistic (72:28). Various sources of carbon, nitrogen, and macroelements were evaluated for medium improvement, and several different types of synthetic macroporous resins were tested to provide the highest virginiamycin titer in culture broth of the developed strain. The resulting improved fermentation medium supplemented with 20 g/L of Amberlite XAD-16 resin increased strain productivity up to 5.03 ± 0.12 g/L with the simultaneous maintenance of the M1:S1 ratio within the synergistic range (72:28) and highly selective level of adsorption of the antibiotic from culture broth (92-95%). The variability of the M1:S1 ratio in the total antibiotic titer depending on various medium composition and resin type was first demonstrated. The obtained strain is promising for the industrial use due to its high productivity and the optimal M1:S1 ratio. Results of the study will be used for the further selection of overproducing strains and the scaling up of the virginiamycin production. The obtained data can be interesting for other researchers working in the field of production of macrolide antibiotics.</p>

scholarly journals Enhancement of β-Mannanase Production by Bacillus subtilis ATCC11774 through Optimization of Medium Composition

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3516 ◽  
Author(s):  
Nor Amalina Binti Mahamad Norizan ◽  
Murni Halim ◽  
Joo Shun Tan ◽  
Sahar Abbasiliasi ◽  
Miskandar Mat Sahri ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Yeast Extract ◽  
Palm Kernel ◽  
Fermentation Medium ◽  
Luria Bertani ◽  
Medium Composition ◽  
Constant Factor ◽  
Nutrient Broth ◽  
Palm Kernel Cake ◽  
Mannanase Activity

Palm kernel cake (PKC) has been largely produced in Malaysia as one of the cheap and abundant agro-waste by-products from the palm oil industry and it contains high fiber (mannan) content. The present study aimed to produce β-mannanase by Bacillus subtilis ATCC11774 via optimization of the medium composition using palm kernel cake as substrate in semi-solid fermentation. The fermentation nutrients such as PKC, peptone, yeast extract, sodium chloride, magnesium sulphate (MgSO2), initial culture pH and temperature were screened using a Plackett-Burman design. The three most significant factors identified, PKC, peptone and NaCl, were further optimized using central composite design (CCD), a response surface methodology (RSM) approach, where yeast extract and MgSO2 were fixed as a constant factor. The maximum β-mannanase activity predicted by CCD under the optimum medium composition of 16.50 g/L PKC, 19.59 g/L peptone, 3.00 g/L yeast extract, 2.72 g/L NaCl and 0.2 g/L MgSO2 was 799 U/mL. The validated β-mannanase activity was 805.12 U/mL, which was close to the predicted β-mannanas activity. As a comparison, commercial media such as nutrient broth, M9 and Luria bertani were used for the production of β-mannanase with activities achieved at 204.16 ± 9.21 U/mL, 50.32 U/mL and 88.90 U/mL, respectively. The optimized PKC fermentation medium was four times higher than nutrient broth. Hence, it could be a potential fermentation substrate for the production of β-mannanase activity by Bacillus subtilis ATCC11774.

scholarly journals Growth and Protein Content Establishment of Pleurotus ostreatus on Liquid and Solid Medium

2017 ◽  
Vol 9 (3) ◽  
pp. 572 ◽  
Author(s):  
Aris Mumpuni ◽  
Nuraeni Ekowati ◽  
Purnomowati Purnomowati ◽  
Endang Sri Purwati
Keyword(s):  
Liquid Medium ◽  
Protein Content ◽  
Pleurotus Ostreatus ◽  
Mycelial Growth ◽  
Corn Starch ◽  
Solid Medium ◽  
Fruit Body ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Increase Protein Content

<p class="IsiAbstrakIndo"><em><span lang="EN-GB">Pleurotus ostreatus</span></em><span lang="EN-GB"> cultivation is performed using solid medium to harvest fruit body and using liquid medium to harvest mycelia in submerged culture. Modifying nutrients in the medium to increase protein content of the fruitbody and mycelia can be done through addition of nitrogen-containing materials. This study aims to determine: the appropriate composition of the liquid medium for high mycelial growth and protein content; and the exact composition of the solid medium to obtain high fruitbody product and protein content. The method was experimental with completely randomized design (CRD). The treatments were incubation of </span><em><span lang="EN-GB">P. ostreatus</span></em><span lang="EN-GB"> on three types of liquid medium and four types of solid medium. The results showed that the optimal liquid medium composition for mycelial  growth was Liquid Fermentation Medium 1 (FC1) with 10% corn fluor, and the highest protein content was in Liquid Fermentation Medium 2 (FC2 = 29.76%). While the optimal solid medium composition for fruitbody production was the medium with 3% corn starch supplement (TJ3), and the highest protein content was obtained from the medium without corn starch supplement (TJ0=24.69%). The increase of mycelial and fruitbody weight from the medium with the addition of corn material indicated a prospective in cultivation process, however effort to increase protein content of the fruit body needs further research. Cultivating </span><em><span lang="EN-GB">P. ostreatus</span></em><span lang="EN-GB"> in mycelial phase may take shorter incubation time, may be produced in mass production with less space consuming, and higher protein content than that by producing fruitbody.</span></p>

scholarly journals Optimization of Fermentation Medium for the Production of Atrazine Degrading StrainAcinetobactersp. DNS32by Statistical Analysis System

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Ying Zhang ◽  
Yang Wang ◽  
Zhi-Gang Wang ◽  
Xi Wang ◽  
Huo-Sheng Guo ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Soybean Flour ◽  
Corn Flour ◽  
Statistical Analysis System ◽  
Shake Flasks ◽  
Burman Design ◽  
Analysis System ◽  
Optimization Of Fermentation

Statistical experimental designs provided by statistical analysis system (SAS) software were applied to optimize the fermentation medium composition for the production of atrazine-degradingAcinetobactersp. DNS32in shake-flask cultures. A “Plackett-Burman Design” was employed to evaluate the effects of different components in the medium. The concentrations of corn flour, soybean flour, and K2HPO4were found to significantly influenceAcinetobactersp. DNS32production. The steepest ascent method was employed to determine the optimal regions of these three significant factors. Then, these three factors were optimized using central composite design of “response surface methodology.” The optimized fermentation medium composition was composed as follows (g/L): corn flour 39.49, soybean flour 25.64, CaCO33, K2HPO43.27, MgSO4·7H2O 0.2, and NaCl 0.2. The predicted and verifiable values in the medium with optimized concentration of components in shake flasks experiments were7.079×108 CFU/mL and7.194×108 CFU/mL, respectively. The validated model can precisely predict the growth of atrazine-degraing bacterium,Acinetobactersp. DNS32.

scholarly journals Influence of Fermentation Medium Composition on Physicochemical Surface Properties of Lactobacillus acidophilus

2005 ◽  
Vol 71 (12) ◽  
pp. 8165-8173 ◽  
Author(s):  
Prisca Schär-Zammaretti ◽  
Marie-Lise Dillmann ◽  
Nicola D'Amico ◽  
Michael Affolter ◽  
Job Ubbink
Keyword(s):  
Cell Wall ◽  
Physicochemical Properties ◽  
Surface Properties ◽  
Yeast Extract ◽  
Lactobacillus Acidophilus ◽  
Bacterial Cell ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Surface Proteins ◽  
Complete Medium

ABSTRACT The effect of the simple and complex basic components of a fermentation medium on the surface properties of Lactobacillus acidophilus NCC2628 is studied by physicochemical methods, such as electrophoresis, interfacial adhesion, and X-ray photonelectron spectroscopy, and by transmission electron microscopy. Starting from an optimized complete medium, the effect of carbohydrates, peptones, and yeast extracts on the physicochemical properties of the cell wall is systematically investigated by consecutively omitting one of the principal components from the fermentation medium at the time. The physicochemical properties and structure of the bacterial cell wall remain largely unchanged if the carbohydrate content of the fermentation medium is strongly reduced, although the concentration of surface proteins increases slightly. Both peptone and yeast extract have a considerable influence on the bacterial cell wall, as witnessed by changes in surface charge, hydrophobicity, and the nitrogen-to-carbon ratio. Both zeta potential and the cell wall hydrophobicity show a positive correlation with the nitrogen-to-carbon ratio of the bacterial surfaces, indicative of the important role of surface proteins in the overall surface physical chemistry. The hydrophobicity of the cell wall, which is low for the cultures grown in the complete medium and in the absence of carbohydrates, becomes fairly high for the cultures grown in the medium without peptones and the medium without yeast extract. UV spectrophotometry and sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with liquid chromatography-tandem mass spectrometry are used to analyze the effect of medium composition on LiCl-extractable cell wall proteins, confirming the major change in protein composition of the cell wall for the culture fermented in the medium without peptones. In particular, it is found that expression of the S-layer protein is dependent on the protein source of the fermentation medium.

Enhanced Biosynthesis of D-arabitol by Metschnikowia Reukaufii Through Optimizing Medium Composition and Fermentation Conditions

2021 ◽  
Author(s):  
Jiaqi Huang ◽  
Yingfeng An ◽  
Hossain M. Zabed ◽  
Yuvaraj Ravikumar ◽  
Mei Zhao ◽  
...  
Keyword(s):  
Fumaric Acid ◽  
Large Scale ◽  
Low Cost ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Optimal Conditions ◽  
Optimum Conditions ◽  
Fermentation Time ◽  
Box Behnken Design ◽  
Fermentation Conditions

Abstract D-arabitol is an important functional sugar alcohol, which can be used in the preparation of foods, chemicals, and medicines. Despite biological production of D-arabitol from low-cost substrates has recently been the focus of research, low yield of this technology has limited its large-scale exploitation. Optimization of this bioprocess could be a promising option to improve the yield of D-arabitol. In this study, one-factor-at-a-time (OFAT) strategy and Box-Behnken design (BBD) were used to increase D-arabitol production by Metschnikowia reukaufii CICC 31858 through optimizing the fermentation conditions and medium composition. The OFAT optimization provided the optimal conditions for temperature, agitation speed, and fermentation time of 30℃, 220 rpm, and 144 h, respectively. Likewise, the optimum concentrations of peptone, ammonium sulfate, KH2PO4, MgSO4·7H2O, and fumaric acid in the fermentation medium were (g/L) 7.5, 1, 2 , 0.5 and 7.5, respectively. Under these optimum conditions, 80.43 g/L of D-arabitol was produced from 200 g/L of glucose, with a productivity of 0.56 g/L/h. The BBD optimization with three important components of fermentation medium (KH2PO4, MgSO4·7H2O and fumaric acid) showed that the predicted titer of D-arabitol varied from 47.21 g/L to 89.27 g/L, and the actual titer of D-arabitol ranged from 47.36 to 89.83 g/L. The optimum concentrations (g/L) of KH2PO4, MgSO4·7H2O, and fumaric acid in the fermentation medium were found to be 1.0, 0.5, and 4.7g/L, respectively. Under the optimum conditions, 92.45 g/L of D-arabitol was finally produced with the yield and productivity of 0.46 g/g and 0.64 g/L/h, respectively.

Sign in / Sign up

Export Citation Format

Share Document