Preparation, spectral properties and biological activities of 5-bromo-6-methyl-2'-deoxyuridine and 5-iodo-6-methyl-2'-deoxyuridine

1980 ◽  
Vol 45 (8) ◽  
pp. 2364-2370 ◽  
Author(s):  
Antonín Holý ◽  
Erik De Clercq
Keyword(s):  
Antiviral Activity ◽  
Spectral Properties ◽  
De Novo ◽  
Biological Activities ◽  
Rabbit Kidney ◽  
Kidney Cells ◽  
Cd Spectra ◽  
Methyl Derivatives ◽  
Primary Rabbit

Reaction of 3',5'-di-O-benzoyl-6-methyl-2'-deoxyuridine (IIa) with elementary bromine or iodine afforded 5-halogeno derivatives IIc and IId which on methanolysis gave 5-bromo-6-methyl-2'-deoxyurine (Ic) and 5-iodo-6-methyl-2'-deoxyurine (Id), respectively. The CD spectra of Ic, Id and 6-methyl-2'-deoxyuridine (Ia) are compared and discussed with regard to determination of the nucleoside conformation. Unlike 5-bromo- and 5-iodo-2'-deoxyuridine, the 6-methyl derivatives Ic and Id exhibit neither antibacterial nor antiviral activity. Nor do they exert any antimetabolic effect on the de novo DNA synthesis in primary rabbit kidney cells.

A New Clerodane Diterpenoid Isolated from Propolis

1995 ◽  
Vol 50 (1-2) ◽  
pp. 93-97 ◽  
Author(s):  
Tetsuya Matsuno
Keyword(s):  
Human Lung ◽  
S Phase ◽  
Rabbit Kidney ◽  
Kidney Cells ◽  
Cell Cytotoxicity ◽  
Cytotoxicity Assays ◽  
Human Lung Carcinoma ◽  
Clerodane Diterpenoid ◽  
Brazilian Propolis ◽  
Primary Rabbit

Abstract A physiologically active substance has been isolated from Brazilian propolis and charac­terized as a new clerodane diterpenoid, as indicated by human hepatocellular carcinoma HuH 13 cell cytotoxicity assays. This compound inhibited growth of the hepatoma cells at a concentration around 10 μg/ml and arrested the tumor cells at S phase as revealed by flow cytometry. At higher concentrations it exerted lethal damage. The compound showed cyto­toxicity on human lung carcinoma HLC-2, HeLa, KB and rat W3Y cells, whereas human diploid foreskin and primary rabbit kidney cells were less affected.

Effects of Low Molecular Weight Fibrinogen Degradation Products on Lymphocytes, Macrophages and Kidney Cells in Cultures

1979 ◽  
Author(s):  
M. Kopeć ◽  
W. Rosazkowski ◽  
S. Szmigielski ◽  
B. Gerdin ◽  
T. Saldeen
Keyword(s):  
Degradation Products ◽  
Biological Activities ◽  
Peritoneal Macrophages ◽  
Rabbit Kidney ◽  
Low Molecular Weight ◽  
Kidney Cells ◽  
Dependent Manner ◽  
Dose Dependent ◽  
Mouse Peritoneal Macrophages ◽  
Molecular Sieving

Dialysable peptides of mw 2200 to 3500 D(LMW-FDP) were isolated from products of exhaustive proteolysis of fibrinogen by plasmin. LMW-FDP inhibited in a dose dependent manner incorporation of 3H-thymidme into cultures of human blood lymphocytes simulated with PHA, concanavalin A or bacterial lipopolisaccharide. These effects were inversely related to stimulation indices. Phagocytosis of 3H-thymidine labelled Staphylococcus aureus by mouse peritoneal macrophages was also inhibited by LMW-FDP. These peptides induced a cytotoxic effect on continuous line of rabbit kidney cells in cultures as manifested by inhibition of 86Rb and 3H-incorporation as well as by releese of 86Rb and 61Cr from prelabelled cells. Eight fractions obtained by molecular sieving of LMW - FDP on Bio-Gel P-6 columns were found to differ pronouncedly in biological activities.

Deoxyribonucleases in Herpes simplex Virus Type 1 and 2 Infected Primary Rabbit Kidney Cells

1980 ◽  
Vol 35 (9-10) ◽  
pp. 747-749
Author(s):  
E. Jürgen Zöllner ◽  
Rudolf K. Zahn ◽  
Dietrich Falke
Keyword(s):  
Enzyme Activity ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Time Course ◽  
Rabbit Kidney ◽  
Kidney Cells ◽  
Infected Cells ◽  
Primary Rabbit ◽  
Simplex Virus

Abstract In primary rabbit kidney cells infected with herpes simplex virus four different neutral deoxyribonuclease activities can be detected by means of the deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by discelectrophoresis. The method is suitable to follow independently the change in each activity of the different enzymes using only about 5 × 105 cells for each assay during the time-course of infection. Under these conditions one enzyme activity is constant, two disappear while the activity of a fourth one present only in infected cells, increases.

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