Reduction of disulfide bridge Cys14-Cys38 of pancreatic trypsin inhibitor by β-mercaptoethanol: Characterization of arising protein derivative by optical dispersion and circular dichroism measurements

1968 ◽  
Vol 33 (7) ◽  
pp. 2299-2306 ◽  
Author(s):  
B. Meloun ◽  
I. Frič ◽  
F. Šorm
Keyword(s):  
Circular Dichroism ◽  
Trypsin Inhibitor ◽  
Disulfide Bridge ◽  
Optical Dispersion ◽  
Pancreatic Trypsin Inhibitor ◽  
Circular Dichroïsm

Characterization of Tamm-Horsfall Urinary Glycoprotein

1973 ◽  
Vol 31 ◽  
pp. 420-421
Author(s):  
John P. Robinson ◽  
J. David Puett
Keyword(s):  
Electron Microscopy ◽  
Circular Dichroism ◽  
Secondary Structure ◽  
Quaternary Structure ◽  
Normal Adult ◽  
Morphological Properties ◽  
Adult Males ◽  
Circular Dichroïsm ◽  
Urinary Glycoprotein

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.

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