scholarly journals Erratum for Caves et al., “Air-Liquid Interface Method To Study Epstein-Barr Virus Pathogenesis in Nasopharyngeal Epithelial Cells”

mSphere ◽  
2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Elizabeth A. Caves ◽  
Sarah A. Cook ◽  
Nara Lee ◽  
Donna Stoltz ◽  
Simon Watkins ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Epstein Barr Virus ◽  
Liquid Interface ◽  
Barr Virus ◽  
Epstein Barr ◽  
Air Liquid Interface ◽  
Nasopharyngeal Epithelial

scholarly journals Air-Liquid Interface Method To Study Epstein-Barr Virus Pathogenesis in Nasopharyngeal Epithelial Cells

mSphere ◽  
2018 ◽  
Vol 3 (4) ◽  
Author(s):  
Elizabeth A. Caves ◽  
Sarah A. Cook ◽  
Nara Lee ◽  
Donna Stoltz ◽  
Simon Watkins ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Infected Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
Culture Method ◽  
Liquid Interface ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr ◽  
Air Liquid Interface

ABSTRACT Epstein-Barr virus (EBV) is a ubiquitous gammaherpesvirus that establishes a latent reservoir in peripheral B-lymphocytes with sporadic reactivation. EBV also infects epithelial cells, predominantly resulting in a lytic infection, which may contribute to EBV transmission from saliva. In the nasopharynx, EBV infection can lead to the clonal expansion of a latently infected cell and the development of nasopharyngeal carcinoma (NPC). The mechanisms governing EBV pathogenesis in nasopharyngeal epithelium are largely unknown. An advanced understanding would depend on a physiologically relevant culture model of polarized airway epithelium. The recent application of the organotypic raft culture in keratinocytes has demonstrated great promise for the use of polarized cultures in the study of EBV permissive replication. In this study, the adaptation of an air-liquid interface (ALI) culture method using transwell membranes was explored in an EBV-infected NPC cell line. In the EBV-infected NPC HK1 cell line, ALI culture resulted in the completion of EBV reactivation, with global induction of the lytic cascade, replication of EBV genomes, and production of infectious progeny virus. We propose that the ALI culture method can be widely adopted as a physiologically relevant model to study EBV pathogenesis in polarized nasal epithelial cells. IMPORTANCE Lifting adherent cells to the air-liquid interface (ALI) is a method conventionally used to culture airway epithelial cells into polarized apical and basolateral surfaces. Reactivation of Epstein-Barr virus (EBV) from monolayer epithelial cultures is sometimes abortive, which may be attributed to the lack of authentic reactivation triggers that occur in stratified epithelium in vivo. In the present work, the ALI culture method was applied to study EBV reactivation in nasopharyngeal epithelial cells. The ALI culture of an EBV-infected cell line yielded high titers and can be dissected by a variety of molecular virology assays that measure induction of the EBV lytic cascade and EBV genome replication and assembly. EBV infection of polarized cultures of primary epithelial cells can be challenging and can have variable efficiencies. However, the use of the ALI method with established EBV-infected cell lines offers a readily available and reproducible approach for the study of EBV permissive replication in polarized epithelia.

scholarly journals Epstein–Barr Virus Infection of Pseudostratified Nasopharyngeal Epithelium Disrupts Epithelial Integrity

Cancers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2722
Author(s):  
Fenggang Yu ◽  
Yanan Lu ◽  
Yingying Li ◽  
Yuji Uchio ◽  
Utomo Andi Pangnguriseng ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Epstein Barr Virus ◽  
Basal Layer ◽  
Infection Model ◽  
Hybridization Technique ◽  
Epstein Barr Virus Infection ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr ◽  
Nasopharyngeal Epithelial

Epstein–Barr virus (EBV) is a human oncogenic virus that causes several types of tumor, such as Burkitt’s lymphoma and nasopharyngeal carcinoma (NPC). NPC tumor cells are clonal expansions of latently EBV-infected epithelial cells. However, the mechanisms by which EBV transforms the nasopharyngeal epithelium is hampered, because of the lack of good in vitro model to pursue oncogenic process. Our primary nasopharyngeal epithelial cell cultures developed pseudostratified epithelium at the air-liquid interface, which was susceptible to EBV infection. Using the highly sensitive RNA in situ hybridization technique, we detected viral infection in diverse cell types, including ciliated cells, goblet cells, and basal cells. EBV-encoded small RNA-positive cells were more frequently detected in the suprabasal layer than in the basal layer. We established the most physiologically relevant EBV infection model of nasopharyngeal epithelial cells. This model will advance our understanding of EBV pathogenesis in the development of NPC.

Expression of Epstein-Barr virus-encoded LMP1 and hTERT extends the life span and immortalizes primary cultures of nasopharyngeal epithelial cells

2010 ◽  
Vol 82 (10) ◽  
pp. 1711-1723 ◽  
Author(s):  
Yim-Ling Yip ◽  
Chi-Man Tsang ◽  
Wen Deng ◽  
Pak-Yan Cheung ◽  
Yuesheng Jin ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Life Span ◽  
Epstein Barr Virus ◽  
Primary Cultures ◽  
Barr Virus ◽  
Epstein Barr ◽  
Nasopharyngeal Epithelial

scholarly journals Transfection of human nasopharyngeal epithelial cells with Epstein-Barr virus DNA derived from nasopharyngeal carcinoma.

1987 ◽  
Vol 90 (9) ◽  
pp. 1334-1338
Author(s):  
TORU TAKIMOTO ◽  
TAMEO MIYAZAKI ◽  
JUNICHI IWAWAKI ◽  
SHIGERU ISHIKAWA ◽  
SAICHIROH TANAKA ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cells ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Nasopharyngeal Epithelial

Transfection of Human Nasopharyngeal Epithelial Cells with Epstein-Barr Virus DNA

ORL ◽  
1987 ◽  
Vol 49 (2) ◽  
pp. 81-86
Author(s):  
T. Takimoto ◽  
H. Sato ◽  
H. Ogura ◽  
S. Ishikawa ◽  
T. Miyazaki
Keyword(s):  
Epithelial Cells ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Nasopharyngeal Epithelial
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