Expression of Epstein-Barr virus-encoded LMP1 and hTERT extends the life span and immortalizes primary cultures of nasopharyngeal epithelial cells

2010 ◽  
Vol 82 (10) ◽  
pp. 1711-1723 ◽  
Author(s):  
Yim-Ling Yip ◽  
Chi-Man Tsang ◽  
Wen Deng ◽  
Pak-Yan Cheung ◽  
Yuesheng Jin ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Life Span ◽  
Epstein Barr Virus ◽  
Primary Cultures ◽  
Barr Virus ◽  
Epstein Barr ◽  
Nasopharyngeal Epithelial

scholarly journals Epstein–Barr Virus Infection of Pseudostratified Nasopharyngeal Epithelium Disrupts Epithelial Integrity

Cancers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2722
Author(s):  
Fenggang Yu ◽  
Yanan Lu ◽  
Yingying Li ◽  
Yuji Uchio ◽  
Utomo Andi Pangnguriseng ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Epstein Barr Virus ◽  
Basal Layer ◽  
Infection Model ◽  
Hybridization Technique ◽  
Epstein Barr Virus Infection ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr ◽  
Nasopharyngeal Epithelial

Epstein–Barr virus (EBV) is a human oncogenic virus that causes several types of tumor, such as Burkitt’s lymphoma and nasopharyngeal carcinoma (NPC). NPC tumor cells are clonal expansions of latently EBV-infected epithelial cells. However, the mechanisms by which EBV transforms the nasopharyngeal epithelium is hampered, because of the lack of good in vitro model to pursue oncogenic process. Our primary nasopharyngeal epithelial cell cultures developed pseudostratified epithelium at the air-liquid interface, which was susceptible to EBV infection. Using the highly sensitive RNA in situ hybridization technique, we detected viral infection in diverse cell types, including ciliated cells, goblet cells, and basal cells. EBV-encoded small RNA-positive cells were more frequently detected in the suprabasal layer than in the basal layer. We established the most physiologically relevant EBV infection model of nasopharyngeal epithelial cells. This model will advance our understanding of EBV pathogenesis in the development of NPC.

scholarly journals Transfection of human nasopharyngeal epithelial cells with Epstein-Barr virus DNA derived from nasopharyngeal carcinoma.

1987 ◽  
Vol 90 (9) ◽  
pp. 1334-1338
Author(s):  
TORU TAKIMOTO ◽  
TAMEO MIYAZAKI ◽  
JUNICHI IWAWAKI ◽  
SHIGERU ISHIKAWA ◽  
SAICHIROH TANAKA ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cells ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Nasopharyngeal Epithelial

Transfection of Human Nasopharyngeal Epithelial Cells with Epstein-Barr Virus DNA

ORL ◽  
1987 ◽  
Vol 49 (2) ◽  
pp. 81-86
Author(s):  
T. Takimoto ◽  
H. Sato ◽  
H. Ogura ◽  
S. Ishikawa ◽  
T. Miyazaki
Keyword(s):  
Epithelial Cells ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
Nasopharyngeal Epithelial
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