scholarly journals Physical linkage of mouse lambda genes by pulsed-field gel electrophoresis suggests that the rearrangement process favors proximate target sequences.

1989 ◽  
Vol 9 (2) ◽  
pp. 711-718 ◽  
Author(s):  
U Storb ◽  
D Haasch ◽  
B Arp ◽  
P Sanchez ◽  
P A Cazenave ◽  
...  
Keyword(s):  
Gene Order ◽  
Gel Electrophoresis ◽  
Gene Locus ◽  
Pulsed Field Gel Electrophoresis ◽  
Immunoglobulin Gene ◽  
Related Gene ◽  
Pulsed Field ◽  
Physical Linkage ◽  
Rearrangement Process ◽  
Target Sequences

The first complete map of a mammalian immunoglobulin gene locus is presented. Mouse lambda genes were mapped by pulsed-field gel electrophoresis. The gene order is V2-Vx-C2-C4-V1-C3-C1. The distance between V2 or Vx and the C2-C4 cluster is 74 or 55 kilobases (kb), respectively, whereas that between V1 and C3-C1 is only 19 kb; V2 and C3-C1 are at least 190 kb apart. Thus, the distances between the lambda subloci are inversely proportional to their frequencies of rearrangement. The related gene lambda 5 is not within the 500 kb of the lambda locus mapped here.

Physical linkage of mouse lambda genes by pulsed-field gel electrophoresis suggests that the rearrangement process favors proximate target sequences

1989 ◽  
Vol 9 (2) ◽  
pp. 711-718
Author(s):  
U Storb ◽  
D Haasch ◽  
B Arp ◽  
P Sanchez ◽  
P A Cazenave ◽  
...  
Keyword(s):  
Gene Order ◽  
Gel Electrophoresis ◽  
Gene Locus ◽  
Pulsed Field Gel Electrophoresis ◽  
Immunoglobulin Gene ◽  
Related Gene ◽  
Pulsed Field ◽  
Physical Linkage ◽  
Rearrangement Process ◽  
Target Sequences

The first complete map of a mammalian immunoglobulin gene locus is presented. Mouse lambda genes were mapped by pulsed-field gel electrophoresis. The gene order is V2-Vx-C2-C4-V1-C3-C1. The distance between V2 or Vx and the C2-C4 cluster is 74 or 55 kilobases (kb), respectively, whereas that between V1 and C3-C1 is only 19 kb; V2 and C3-C1 are at least 190 kb apart. Thus, the distances between the lambda subloci are inversely proportional to their frequencies of rearrangement. The related gene lambda 5 is not within the 500 kb of the lambda locus mapped here.

Partial deletions of a sequence family (“DXS278”) and its physical linkage to steroid sulfatase as detected by pulsed-field gel electrophoresis

Genomics ◽  
1990 ◽  
Vol 8 (2) ◽  
pp. 255-262 ◽  
Author(s):  
Rhonda E. Schnur ◽  
Robert G. Knowlton ◽  
Maria A. Musarella ◽  
Maximilian Muenke ◽  
Robert L. Nussbaum
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Steroid Sulfatase ◽  
Sequence Family ◽  
Pulsed Field ◽  
Physical Linkage

Analysis of YAC clones by pulsed-field gel electrophoresis: Physical mapping of Cu/Zn superoxide dismutase gene locus

Methods ◽  
1990 ◽  
Vol 1 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Takashi Imai ◽  
Aritoshi Iida ◽  
Tokiko Miwa ◽  
Hiroyuki Tashiro ◽  
Jae-Chan Song ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Physical Mapping ◽  
Gel Electrophoresis ◽  
Gene Locus ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Superoxide Dismutase Gene

scholarly journals Use of Pulsed-Field Gel Electrophoresis to Determine the Source of Methicillin-Resistant Staphylococcus aureus Bacteremia

2021 ◽  
Vol 13 (3) ◽  
pp. 602-610
Author(s):  
Eugene Y. H. Yeung ◽  
Ivan Gorn
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Epidemiological Studies ◽  
Pelvic Trauma ◽  
Pulsed Field Gel Electrophoresis ◽  
Bacterial Strains ◽  
Methicillin Resistant ◽  
Pulsed Field ◽  
Clinical Cases

Pulsed-field gel electrophoresis (PFGE) has historically been considered the gold standard in fingerprinting bacterial strains in epidemiological studies and outbreak investigations; little is known regarding its use in individual clinical cases. The current study detailed two clinical cases in which PFGE helped to determine the source of their methicillin-resistant Staphylococcus aureus (MRSA) bacteremia. Patient A was found to have MRSA bacteremia after trauma in her pelvic area. MRSA was also found in her groin but not in her nostril and rectum. PFGE was performed that showed variable bands of her MRSA isolates from blood and groin, suggestive of different strains of MRSA. Her MRSA bacteremia was determined to be unrelated to her pelvic trauma. Patient B was found to have MRSA bacteremia after colonoscopy. MRSA was also found in his nostril and rectum. PFGE was performed that showed variable bands of his MRSA isolates from blood and rectum but identical bands of MRSA isolates from his blood and nostril. His MRSA bacteremia was determined to be unrelated to his colonoscopy procedure. The current study demonstrates the use of PFGE to rule out the source of bacteremia in individual clinical cases.

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