scholarly journals Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients

2016 ◽  
Vol 54 (6) ◽  
pp. 1641-1643 ◽  
Author(s):  
Sylvie Zida ◽  
Edouard Tuaillon ◽  
Makoura Barro ◽  
Arnaud Kwimatouo Lekpa Franchard ◽  
Thérèse Kagoné ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Dried Blood Spots ◽  
Peripheral Blood Mononuclear ◽  
Rna Detection ◽  
Blood Spots ◽  
Hiv Rna ◽  
Rna Quantification ◽  
Blood Mononuclear Cells ◽  
The Impact ◽  
Hiv 1

The impact of HIV-1 DNA coamplification during HIV-1 RNA quantification on dried blood spots (DBS) was explored. False-positive HIV RNA detection (22/62, 35%) was associated with high HIV-1 DNA levels. Specificity of HIV-1 RNA assays on DBS should be evaluated following manufacturer protocols on samples with HIV-1 DNA levels of ≥1,000 copies/106peripheral blood mononuclear cells.

scholarly journals Evaluation of the Aptima HIV-1 Quant Dx Assay for HIV-1 RNA Quantitation in Different Biological Specimen Types

2017 ◽  
Vol 55 (8) ◽  
pp. 2544-2553 ◽  
Author(s):  
Christina Yek ◽  
Marta Massanella ◽  
Tashi Peling ◽  
Kristen Lednovich ◽  
Sangeetha V. Nair ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Mononuclear Cells ◽  
Dried Blood Spots ◽  
Proteinase K ◽  
Peripheral Blood Mononuclear ◽  
Detection Rates ◽  
Hiv Rna ◽  
Resource Limited ◽  
Hiv 1 ◽  
Rna Levels

ABSTRACTThe search for a cure for HIV infection has highlighted the need for increasingly sensitive and precise assays to measure viral burden in various tissues and body fluids. We describe the application of a standardized assay for HIV-1 RNA in multiple specimen types. The fully automated Aptima HIV-1 Quant Dx assay (Aptima assay) is FDA cleared for blood plasma HIV-1 RNA quantitation. In this study, the Aptima assay was applied for the quantitation of HIV RNA in peripheral blood mononuclear cells (PBMCs;n= 72), seminal plasma (n= 20), cerebrospinal fluid (CSF;n= 36), dried blood spots (DBS;n= 104), and dried plasma spots (DPS;n= 104). The Aptima assay was equivalent to or better than commercial assays or validated in-house assays for the quantitation of HIV RNA in CSF and seminal plasma. For PBMC specimens, the sensitivity of the Aptima assay in the detection of HIV RNA decayed as background uninfected PBMC counts increased; proteinase K treatment demonstrated some benefit in restoring signal at higher levels of background PBMCs. Finally, the Aptima assay yielded 100% detection rates of DBS in participants with plasma HIV RNA levels of ≥35 copies/ml and 100% detection rates of DPS in participants with plasma HIV RNA levels of ≥394 copies/ml. The Aptima assay can be applied to a variety of specimens from HIV-infected subjects to measure HIV RNA for studies of viral persistence and cure strategies. It can also detect HIV in dried blood and plasma specimens, which may be of benefit in resource-limited settings.

Evidence for late stage compartmentalization of HIV-1 resistance mutations between lymph node and peripheral blood mononuclear cells

AIDS ◽  
2000 ◽  
Vol 14 (15) ◽  
pp. 2273-2281 ◽  
Author(s):  
Da' N. Haddad ◽  
Christopher Birch ◽  
Tracy Middleton ◽  
Dominic E. Dwyer ◽  
Anthony L. Cunningham ◽  
...  
Keyword(s):  
Lymph Node ◽  
Peripheral Blood Mononuclear Cells ◽  
Late Stage ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Resistance Mutations ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document